Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(1), P. 228 - 228
Published: Jan. 19, 2024
Maintaining
proper
mechanical
strength
and
tissue
volume
is
important
for
bone
growth
at
the
site
of
a
defect.
In
this
study,
potassium
magnesium
phosphate
hexahydrate
(KMgPO4·6H2O,
MPC)
was
applied
to
gelma-methacrylate
hydrogel
(GelMA)
prepare
GelMA/MPC
composites
(GMPCs).
Among
these,
5
GMPC
showed
best
performance
in
vivo
vitro.
These
combinations
significantly
enhanced
GelMA
regulated
degradation
absorption
rate
MPC.
Considerably
better
properties
were
noted
compared
with
other
concentrations.
Better
bioactivity
osteogenic
ability
also
found
GMPC.
Magnesium
ions
(Mg2+)
are
bioactive
proven
promote
regeneration,
which
enhancement
efficiency
closely
related
Mg2+
findings
indicated
that
GMPCs
can
release
effective
treatment
defects
hold
promise
future
applications.
Bioactive Materials,
Journal Year:
2024,
Volume and Issue:
37, P. 348 - 377
Published: April 23, 2024
Setting
time
as
the
fourth
dimension,
4D
printing
allows
us
to
construct
dynamic
structures
that
can
change
their
shape,
property,
or
functionality
over
under
stimuli,
leading
a
wave
of
innovations
in
various
fields.
Recently,
smart
biomaterials,
biological
components,
and
living
cells
into
3D
constructs
with
effects
has
led
an
exciting
field
bioprinting.
bioprinting
gained
increasing
attention
is
being
applied
create
programmed
cell-laden
such
bone,
cartilage,
vasculature.
This
review
presents
overview
on
for
engineering
tissues
organs,
followed
by
discussion
approaches,
technologies,
biomaterials
design,
bioink
requirements,
applications.
While
much
progress
been
achieved,
complex
process
facing
challenges
need
be
addressed
transdisciplinary
strategies
unleash
full
potential
this
advanced
biofabrication
technology.
Finally,
we
present
future
perspectives
rapidly
evolving
bioprinting,
view
its
potential,
increasingly
important
roles
development
basic
research,
pharmaceutics,
regenerative
medicine.
Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 5, 2025
Engineered
living
systems
(ELSs)
represent
purpose-driven
assemblies
of
components,
encompassing
cells,
biomaterials,
and
active
agents,
intricately
designed
to
fulfill
diverse
biomedical
applications.
Gelatin
its
derivatives
have
been
used
extensively
in
ELSs
owing
their
mature
translational
pathways,
favorable
biological
properties,
adjustable
physicochemical
characteristics.
This
review
explores
the
intersection
gelatin
with
fabrication
techniques,
offering
a
comprehensive
examination
synergistic
potential
creating
for
various
applications
biomedicine.
It
offers
deep
dive
into
gelatin,
including
structures
production,
sources,
processing,
properties.
Additionally,
techniques
employing
derivatives,
generic
microfluidics,
3D
printing
methods.
Furthermore,
it
discusses
based
on
regenerative
engineering
as
well
cell
therapies,
bioadhesives,
biorobots,
biosensors.
Future
directions
challenges
are
also
examined,
highlighting
emerging
trends
areas
improvements
innovations.
In
summary,
this
underscores
significance
gelatin-based
advancing
lays
groundwork
guiding
future
research
developments
within
field.
Abstract
With
the
aging
and
obesity
era,
increasing
incidence
of
diabetes
diabetic
complications,
especially
non‐healing
wounds,
imposes
a
serious
economic
burden
on
both
patients
society.
The
complex
microenvironments,
including
hyperglycemia,
bacterial
infection,
ischemia,
nerve
damage,
lead
to
prolonged
inflammation
proliferation
phase
wounds.
Mesenchymal
stem
cell‐derived
small
extracellular
vesicles
(MSC‐sEVs),
which
contain
rich
variety
therapeutic
molecules,
have
been
chased
for
decades
because
their
potential
roles
in
cellular
communication,
tissue
regeneration,
drug
delivery.
As
powerful
tools
controlled‐sustained
release
sEVs,
biocompatible
hydrogels
applied
wide
range
biomedical
applications.
Herein,
we
first
summarize
pathological
features
such
as
angiopathy,
neuropathy,
immune
cell
dysfunction.
Then,
discuss
biological
properties,
performance,
stability
pure
MSC‐sEVs.
After
that,
components,
application
patterns,
responsiveness
hydrogels.
Next,
loading
avenues
MSC‐sEVs
into
hydrogel,
behaviors
sEVs
from
hydrogels,
influence
crosslinking
method
hydrogel‐sEV
composites.
Finally,
provide
an
overview
current
applications
loaded
with
novel
cell‐free
engineering
system
managing
wounds
propose
critical
unsolved
issues.
This
review
is
expected
meaningful
guidance
developing
wound
management.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(6), P. 6772 - 6788
Published: Jan. 31, 2024
Owing
to
dysfunction
of
the
uterus,
millions
couples
around
world
suffer
from
infertility.
Different
conventional
treatments,
tissue
engineering
provides
a
new
and
promising
approach
deal
with
difficult
problems
such
as
human
or
organ
failure.
Adopting
scaffold-based
engineering,
three-dimensional
(3D)
porous
scaffolds
in
combination
stem
cells
appropriate
biomolecules
may
be
constructed
for
uterine
regeneration.
In
this
study,
hierarchical
scaffold,
which
mimicked
structure
functions,
was
designed,
biomimicking
were
then
successfully
fabricated
using
solvent
casting,
layer-by-layer
assembly,
3D
bioprinting
techniques.
For
multilayered,
structured
scaffolds,
poly(l-lactide-co-trimethylene
carbonate)
(PLLA-co-TMC,
"PLATMC"
short)
poly(lactic
acid-co-glycolic
acid)
(PLGA)
blends
first
used
fabricate
shape-morphing
layer
mimic
function
myometrium
tissue.
The
PLATMC/PLGA
polymer
blend
highly
stretchable.
Subsequently,
after
etching
surface
employing
estradiol
(E2),
polydopamine
(PDA),
hyaluronic
acid
(HA),
PDA@E2/HA
multilayer
films
formed
on
build
an
intelligent
delivery
platform
enable
controlled
sustained
release
E2.
also
improved
biological
performance
scaffold.
Finally,
bone
marrow-derived
mesenchymal
cell
(BMSC)-laden
hydrogel
[which
gelatin
methacryloyl
(GelMA)
(Gel)]
printed
thereby
completing
construction
BMSCs
GelMA/Gel
exhibited
excellent
viability
could
spread
released
eventually
upon
biodegradation
hydrogel.
It
shown
that
hierarchically
evolve
initial
flat
shape
into
tubular
completely
aqueous
environment
at
37
°C,
fulfilling
requirement
curved
engineering.
shape,
high
stretchability,
E2
appear
very
Exploration,
Journal Year:
2024,
Volume and Issue:
4(5)
Published: April 17, 2024
Abstract
Scaffold‐based
tissue
engineering
provides
an
efficient
approach
for
repairing
uterine
defects
and
restoring
fertility.
In
the
current
study,
a
novel
trilayer
scaffold
with
high
similarity
to
in
structure
was
designed
fabricated
via
4D
printing,
electrospinning
3D
bioprinting
regeneration.
Highly
stretchable
poly(
l
‐lactide‐
co
‐trimethylene
carbonate)
(PLLA‐
‐TMC,
“PTMC”
short)/thermoplastic
polyurethane
(TPU)
polymer
blend
scaffolds
were
firstly
made
printing.
To
improve
biocompatibility,
porous
poly(lactic
acid‐
‐glycolic
acid)
(PLGA)/gelatin
methacryloyl
(GelMA)
fibers
incorporated
polydopamine
(PDA)
particles
produced
on
PTMC/TPU
electrospinning.
Importantly,
estradiol
(E2)
encapsulated
PDA
particles.
The
bilayer
thus
could
provide
controlled
sustained
release
of
E2.
Subsequently,
bone
marrow
derived
mesenchymal
stem
cells
(BMSCs)
mixed
gelatin
(GelMA)‐based
inks
formulated
bioinks
used
fabricate
cell‐laden
hydrogel
layer
bioprinting,
forming
ultimately
biomimicking
formed
exhibited
shape
morphing
ability
by
transforming
from
planar
tubular
structures
when
immersed
culture
medium
at
37°C.
under
development
would
new
insights
Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
19(4), P. 042008 - 042008
Published: May 20, 2024
Gelatin
methacryloyl
(GelMA)
hydrogels
have
gained
significant
recognition
as
versatile
biomaterials
in
the
biomedical
domain.
GelMA
emulate
vital
characteristics
of
innate
extracellular
matrix
by
integrating
cell-adhering
and
metalloproteinase-responsive
peptide
motifs.
These
features
enable
cellular
proliferation
spreading
within
GelMA-based
hydrogel
scaffolds.
Moreover,
displays
flexibility
processing,
it
experiences
crosslinking
when
exposed
to
light
irradiation,
supporting
development
with
adjustable
mechanical
characteristics.
The
drug
delivery
landscape
has
been
reshaped
hydrogels,
offering
a
favorable
platform
for
controlled
sustained
release
therapeutic
actives.
tunable
physicochemical
precise
modulation
kinetics
release,
ensuring
optimal
effectiveness.
In
tissue
engineering,
perform
an
essential
role
design
scaffold,
providing
biomimetic
environment
conducive
cell
adhesion,
proliferation,
differentiation.
Incorporating
three-dimensional
printing
further
improves
its
applicability
developing
complicated
constructs
spatial
precision.
Wound
healing
applications
showcase
bioactive
dressings,
fostering
microenvironment
regeneration.
inherent
biocompatibility
provide
efficiency
closure
wounds
repair.
stand
at
forefront
innovation,
addressing
diverse
challenges
delivery,
wound
healing.
This
review
provides
comprehensive
overview,
in-depth
understanding
hydrogel's
potential
impact
on
progressing
sciences.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(33)
Published: June 17, 2024
Excessive
cell-free
DNA
(cfDNA)
can
induce
chronic
inflammation
by
activating
intracellular
nucleic
acid
sensors.
Intervention
in
cfDNA-mediated
"pro-inflammatory
signaling
transduction"
could
be
a
potential
alleviating
strategy
for
inflammation,
such
as
diabetic
wounds.
However,
effectively
and
specifically
downgrading
cfDNA
concentration
the
pathological
microenvironment
remains
challenge.
Therefore,
this
work
prepares
free-standing
polydopamine
nanosheets
through
DNA-guided
assembly
loaded
them
into
microfluidic
hydrogel
microspheres.
The
π─π
stacking/hydrogen
bonding
interactions
between
π-rich
bases
of
cfDNA,
along
with
cage-like
spatial
confinement
created
polymer
network,
achieved
capture
storage,
respectively.
Catechol
also
assist
reducing
reactive
oxygen
species
(ROS)
levels.
Efficient
binding
independent
serum
proteins,
specific
interdiction
abnormal
activation
cfDNA-associated
toll-like
receptor
9,
well
down-regulation
inflammatory
cytokines
ROS
levels
are
shown
system.
pro-healing
effects
on
mice
model
wounds
investigated.
This
presents
new
capturing
storing
to
intervene
cell
transduction.
It
offers
insights
regulatory
mechanisms
mediators
biomaterials
inflammation-related
diseases.
Biofabrication,
Journal Year:
2024,
Volume and Issue:
16(2), P. 025022 - 025022
Published: Feb. 19, 2024
Versatile
and
efficient
regulation
of
the
mechanical
properties
extracellular
matrix
is
crucial
not
only
for
understanding
dynamic
changes
in
biological
systems,
but
also
obtaining
precise
effective
cellular
responses
drug
testing.
In
this
study,
we
developed
a
well
plate-based
hydrogel
photo-crosslinking
system
to
effectively
control
hydrogels
perform
high-throughput
assays.
We
improved
cell
biocompatibility
by
using
gelatin
methacryloyl
(GelMA)
with
visible
light
method.
Multiple
cell-laden
GelMA
were
simultaneously
uniformly
created
multi-arrayed
520
nm
light-emitting
diodes
plate
format.
The
elastic
modulus
can
be
widely
adjusted
(0.5-30
kPa)
capable
independently
controlling
intensity
or
exposure
time
multiple
samples.
demonstrate
feasibility
our
observing
enhanced
bone
differentiation
human
mesenchymal
stem
cells
(hMSCs)
cultured
on
stiffer
hydrogels.
Additionally,
observed
that
osteogenic
fate
hMSCs,
affected
different
gel,
was
regulated
parathyroid
hormone
(PTH).
Notably,
response
PTH,
hMSCs
high-stiffness
microenvironment
upregulate
while
exhibiting
increased
proliferation
low-stiffness
microenvironment.
Overall,
enables
generation
three-dimensional
culture
models
diverse
holds
significant
potential
expansion
into
