Advancements in Aptamer‐Driven DNA Nanostructures for Precision Drug Delivery

Advanced Science, Journal Year: 2024, Volume and Issue: 11(26)

Published: May 7, 2024

Abstract DNA nanostructures exhibit versatile geometries and possess sophisticated capabilities not found in other nanomaterials. They serve as customizable nanoplatforms for orchestrating the spatial arrangement of molecular components, such biomolecules, antibodies, or synthetic This is achieved by incorporating oligonucleotides into design nanostructure. In realm drug delivery to cancer cells, there a growing interest active targeting assays enhance efficacy selectivity. The approach involves “key‐lock” mechanism where carrier, through its ligand, recognizes specific receptors on tumor facilitating release drugs. Various nanostructures, including origami, Tetrahedral, nanoflower, cruciform, nanostar, nanocentipede, nanococklebur, can traverse lipid layer cell membrane, allowing cargo nucleus. Aptamers, easily formed vitro, are recognized their targeted due high selectivity targets low immunogenicity. review provides comprehensive overview recent advancements formation modification aptamer‐modified within systems.

Language: Английский

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Programmable Tetrahedral DNA‐RNA Nanocages Woven with Stimuli‐Responsive siRNA for Enhancing Therapeutic Efficacy of Multidrug‐Resistant Tumors

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 25, 2024

Abstract Multidrug resistance (MDR) is a major obstacle limiting the effectiveness of chemotherapy against cancer. The combination strategy chemotherapeutic agents and siRNA targeting drug efflux has emerged as an effective cancer treatment to overcome MDR. Herein, stimuli‐responsive programmable tetrahedral DNA‐RNA nanocages (TDRN) have been rationally designed developed for dynamic co‐delivery doxorubicin P‐glycoprotein (P‐gp) siRNA. Specifically, sense antisense strand sequences P‐gp siRNA, which are bricks with terminal disulfide bond conjugation, precisely embedded in one edge DNA tetrahedron. TDRN provides release element control functional cargo that significantly more stable than “tail‐like” TDN nanostructures. highly rigid 3D nanostructure siRNA‐organized demonstrated notable improvement stability RNase A mouse serum, well long‐term storage up 4 weeks, evidenced by this study. These biocompatible multifunctional nanocarriers gold nanocluster‐assisted delivery (TDRN@Dox@AuNC p ) successfully used achieve synergistic RNAi/Chemo‐therapy vitro vivo. This system, integrates RNAi therapy chemotherapy, offers promising approach treating multidrug‐resistant tumors.

Language: Английский

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Ultrasensitive terahertz microfluidic biosensor integrated with tetrahedral DNA nanostructure for specific detection of live cancer cells

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 428, P. 137252 - 137252

Published: Jan. 11, 2025

Language: Английский

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Biotechnological Tools Boost the Functional Diversity of DNA-based Data Storage Systems

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: 27, P. 624 - 630

Published: Jan. 1, 2025

DNA-based data storage has emerged as a groundbreaking solution to the growing demand for efficient, high-density, and long-term storage. It is attracting many researchers' attention, who are implementing functions such random access, searching, operations apart from existing capabilities, including reading writing. We summarize recent progress of how biotechnological tools, based on sequence specificity, encapsulation, high-dimensional structures DNA molecules, facilitate implementation various functions. The limitations using biochemical reactions that hinder development more precise efficient information systems also discussed. Future advancements in molecular biology nanotechnology expected improve architecture, scalability, efficiency storage, positioning it sustainable dynamic alternative conventional systems.

Language: Английский

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Targeting cGAS-STING pathway for reprogramming tumor-associated macrophages to enhance anti-tumor immunotherapy

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: March 12, 2025

Abstract The cyclic GMP-AMP synthase (cGAS)-stimulator interferon genes (STING) signaling pathway plays a crucial role in activating innate and specific immunity anti-tumor immunotherapy. As the major infiltrating cells tumor microenvironment (TME), tumor-associated macrophages (TAMs) could be polarized into either M1 or pro-tumor M2 types based on various stimuli. Accordingly, targeted reprogramming TAMs to restore immune balance shows promise as an effective strategy. In this review, we aim target cGAS-STING for enhance We investigated double-edged sword effects of regulating TME. regulative roles its impact TME were further revealed. More importantly, several strategies targeting designed enhancing Taken together, might promising strategy

Language: Английский

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“Cicada Out of the Shell” Deep Penetration and Blockage of the HSP90 Pathway by ROS‐Responsive Supramolecular Gels to Augment Trimodal Synergistic Therapy

Advanced Science, Journal Year: 2024, Volume and Issue: 11(25)

Published: April 22, 2024

Abstract Deep penetration and downregulation of heat shock protein (HSP) expression in multimodal synergistic therapy are promising approaches for curing cancer clinical trials. However, free small‐molecule drugs most drug vehicles have a low delivery efficiency deep into the tumor owing to poor hypoxic conditions at site. In this study, objective is use reactive oxygen species (ROS)‐responsive supramolecular gels co‐loaded with photosensitizer Zn(II) phthalocyanine tetrasulfonic acid (ZnPCS 4 ) functionalized tetrahedral DNA (TGSAs) (G@P/TGSAs) enhance tissue cell block HSP90 pathway chemo‐ photodynamic (PDT) ‐ photothermal (PTT) trimodal therapy. The injected situ release ZnPCS TGSAs under high ROS concentrations originating from both PDT. penetrate deeply tissues augment by inhibiting pathway. Proteomics show that HSP‐related proteins molecular chaperones inhibited/activated, Simultaneously, TGSA‐regulated apoptotic activated. vivo study demonstrates efficient excellent (45% growth inhibition).

Language: Английский

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Nano revolution of DNA nanostructures redefining cancer therapeutics—A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 274, P. 133244 - 133244

Published: June 18, 2024

Language: Английский

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CpG and Rutin Co‐Loaded DNA Tetrahedra for Targeted Therapy of Intracerebral Hemorrhage: Synergistic Hematoma Clearance and Neuroinflammation Inhibition

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 26, 2024

Abstract Intracerebral hemorrhage (ICH) presents a formidable challenge due to its high mortality and disability rates, primarily attributed cerebral hematoma formation ensuing neuroinflammation. Swift removal is paramount for prognosis, yet existing interventions carry risks limitations. Notably, elevated CD47 expression on hematoma‐associated RBC triggers “don't eat me” signal, impeding clearance, while microglial/macrophage erythrophagocytosis exacerbates oxidative stress the lysate evokes To address this conundrum, multifunctional nanomedicine (TD‐CFR), employing DNA tetrahedra (TD) as carrier ICH treatment introduced. The investigations reveal that CpG enhances phagocytosis of CD47‐expressing by microglia/macrophages via lipid metabolism modulation. Integration into TD preserves pro‐phagocytic efficacy, TD's double‐stranded region enables efficient encapsulation Rutin, potent anti‐inflammatory antioxidant flavonoid. Capitalizing disrupted blood‐brain barrier integrity at site, TD‐CFR achieves robust enrichment within post‐intravenous administration, augmented folate receptor‐mediated targeting microglia/macrophages. Efficacy assessments in mouse rabbit models confirm TD‐CFR's therapeutic benefits, including neuroinflammation suppression, brain function restoration. Leveraging biosafety profile dual active ingredient loading capacity, study unveils promising drug paradigm ICH.

Language: Английский

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Nanosized DNA Hydrogel Functionalized with a DNAzyme Tetrahedron for Highly Efficient Gene Silencing

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(8), P. 4913 - 4924

Published: July 4, 2024

DNAzymes are DNA oligonucleotides that have catalytic activity without the assistance of protein enzymes. In particular, RNA-cleaving were considered as ideal candidates for gene therapy due to their unique characteristics. Nevertheless, efforts use DNAzyme a therapeutic agent limited by issues such low physiological stability in serum and intracellular delivery efficiency. this study, we developed nanosized synthetic hydrogel functionalized with tetrahedron (TDz Dgel) overcome these limitations. We observed remarkable improvement gene-silencing effect well uptake support transfection reagents using TDz Dgel. The improved resulted from combination cell-penetrating structure high hydrogel. envision approach will become convenient efficient strategy future.

Language: Английский

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A tetrahedral DNA nanostructure-mediated miRNA inhibitor delivery system: Type H vessel-related bone healing during distraction osteogenesis

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 153863 - 153863

Published: July 20, 2024

Language: Английский

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