Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

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Engineered extracellular vesicles with sequential cell recruitment and osteogenic functions to effectively promote senescent bone repair

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 12, 2025

Senescent mandibular bone repair poses a formidable challenge without completely satisfactory strategy. Endogenous cell recruitment and osteogenic differentiation are two sequential stages in regeneration, disruptions these processes present significant obstacles to senescent repair. To address issues, engineered extracellular vesicles (EV) with stem functions were developed. This study demonstrated that Apt19s-engineered (Apt19s-EV) recognize recruit marrow mesenchymal cells derived from old rats (O-BMSCs) specifically effectively. MiR-376b-5p, identified by RNA sequencing transfection, was significantly decreased O-BMSCs, it selected construct miR-376b-5p-engineered (376b-EV). 376b-EV could promote osteogenesis alleviate senescence of O-BMSCs targeting Camsap1. combine the advantages Apt19s miR-376b-5p, dual (Apt-376b-EV) comprising both miR-376b-5p modifications constructed. further validate its function, Gelatin methacryloyl (GelMA) hydrogel used as carrier Apt-376b-EV@GelMA delivery system. The vitro results have sequentially. Notably, vivo also showed sequentially endogenous enhance new formation fracture critical-sized defect models. In summary, vesicles, Apt-376b-EV, offer an appealing solution for recruiting promoting microenvironment, which may broaden clinical applications EV provide valuable strategies treating bone-related diseases future work.

Language: Английский

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Continuous‐Gradient Mineralized Hydrogel Synthesized via Gravitational Osmosis for Osteochondral Defect Repair

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Abstract The repair of hierarchical osteochondral defects requires complex gradient reconstruction at different levels, with continuous‐gradient mineralization being crucial. Strategies for achieving have rarely been reported. Here, a mineralized hydrogel is prepared using simple gravitational osmosis ≈2 nm amorphous calcium phosphate nanocluster (ACPC) suspension into composite organic frameworks regeneration. During infiltration, ACPC underwent gradual mineralization, resulting in the spontaneous formation hydroxyapatite (HAP). aligned closely normal structure, thereby effectively promoting cartilage and subchondral bone. In layer, compounds improved oxidative stress environment induced by injury complemented extracellular matrix cartilage. bone HAP from continuously intracellular accumulation mesenchymal stem cells (MSCs), activating calcium/calmodulin‐dependent protein kinase 2 (CaMK2) osteogenic differentiation MSCs through signaling pathway. Ultimately, compared nongradient hydrogel, this innovative method exhibited significantly enhanced regeneration capabilities defects, as evidenced metrics, such volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), number (Tb.N). This holistic strategy provides hope field repair.

Language: Английский

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Novel injectable adhesive hydrogel loaded with exosomes for holistic repair of hemophilic articular cartilage defect

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 85 - 111

Published: Aug. 29, 2024

Hemophilic articular cartilage damage presents a significant challenge for surgeons, characterized by recurrent intraarticular bleeding, severe inflammatory microenvironment, and limited self-repair capability of tissue. Currently, there is lack tissue engineering-based integrated therapies that address both early hemostasis, anti-inflammation, long-lasting chondrogenesis hemophilic defects. Herein, we developed an adhesive hydrogel using oxidized chondroitin sulfate gelatin, loaded with exosomes derived from bone marrow stem cells (BMSCs) (Hydrogel-Exos). This demonstrated favorable injectability, self-healing, biocompatibility, biodegradability, swelling, frictional mechanical properties, providing comprehensive approach to treating The hydrogel, featuring dynamic Schiff base bonds hydrogen bonds, exhibited excellent wet adhesiveness hemostatic properties. In pig model, the could be smoothly injected into knee joint defect site gelled

Language: Английский

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A deformable SIS/HA composite hydrogel coaxial scaffold promotes alveolar bone regeneration after tooth extraction

Bioactive Materials, Journal Year: 2024, Volume and Issue: 46, P. 97 - 117

Published: Dec. 10, 2024

Language: Английский

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Exosomal non-coding RNAs in the regulation of bone metabolism homeostasis: molecular mechanism and therapeutic potential

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e41632 - e41632

Published: Jan. 1, 2025

Bone metabolism is a dynamic balance between bone formation and absorption regulated by osteoblasts/osteoclasts. metabolic disorders can lead to disease. Osteoporosis (OP), osteoarthritis (OA) femoral head necrosis (ONFH) are common diseases. At present, the treatment of disease still mainly relieve pain improve joint function. However, surgical does not apply vast majority high-risk groups, including postmenopausal women, patients with diabetes, cirrhosis, etc. Exosomes (Exos) nanoscale membrane vesicles that released almost all cells. Exos rich in variety bioactive substances, such as non-coding RNAs, nucleic acids, proteins lipids. In view structure Exos, it protect biologically active molecules be smoothly delivered target cells involved regulation cell this review, we focus on mechanism function homeostasis mediated exosomal ncRNAs (Exos-ncRNAs), macrophage polarization, autophagy, angiogenesis, signal transduction competing endogenous RNA (ceRNA). We summarized therapeutic strategies potential drugs Exos-ncRNAs Moreover, discussed shortcomings research directions carrier deliver play role.

Language: Английский

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Functionalized GelMA/CMCS Composite Hydrogel Incorporating Magnesium Phosphate Cement for Bone Regeneration

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 257 - 257

Published: Jan. 21, 2025

Background: Bone regeneration remains a challenging issue in tissue engineering. The use of hydrogels as scaffolds for bone repair has gained attention due to their biocompatibility and ability mimic the extracellular matrix. This study aims develop functionalized GelMA/CMCS composite hydrogel incorporating magnesium phosphate cement (MPC) enhanced regeneration. Methods: These composites were developed by potassium hexahydrate (KMgPO4·6H2O, MPC) powders into methacrylated gelatin/carboxymethyl chitosan (GelMA-C) hydrogels. material’s mechanical properties, antibacterial performance, cytocompatibility evaluated. In vitro experiments involved cell viability osteogenic differentiation assays using rBMSCs well angiogenic potential HUVECs. was also assessed its promoting rat (Sprague-Dawley) model defect. Results: GelMA-CM demonstrated improved biocompatibility, compared individual GelMA or CMCS Incorporation MPC facilitated sustained release ions which promoted pre-osteoblasts. vivo results indicated accelerated healing defect model. Conclusions: could be viable candidate clinical applications therapies.

Language: Английский

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Haversian bone mimicking scaffold with built-in vasculature for bone tissue engineering

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160023 - 160023

Published: Feb. 1, 2025

Language: Английский

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Exosomes in the Chemoresistance of Glioma: Key Point in Chemoresistance

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)

Published: Feb. 1, 2025

ABSTRACT Gliomas are the most ordinary primary virulent brain tumours and commonly used clinical treatments include tumour resection, radiation therapy chemotherapy. Although significant progress has been made in recent years progression‐free survival (PFS) overall (OS) for patients with high‐grade gliomas, prognosis remains poor. Chemoresistance refers to phenomenon of decreased sensitivity cells drugs, resulting reduced or ineffective drug efficacy, is an important cause failure Exosomes, a type extracellular vesicle, secreted by cancer various stromal microenvironment (TME) transfer their inclusions cells, increasing chemoresistance. Furthermore, depletion exosomes reverses certain detrimental effects on metabolism restores chemotherapeutic agents. Here, we summarised correlation between resistance agents glioma patients, mechanisms action involved value. We aimed afford new thoughts research, diagnosis intervention chemoresistance patients.

Language: Английский

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Injectable Hydrogels Based on Hyperbranched Polymers for Biomedical Applications

Chem & Bio Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Injectable hydrogels (IHs) have garnered significant attention in biomedical applications due to their minimally invasive nature, adaptability, and high degree of customization. However, traditional design methods IHs limitations addressing complex clinical needs, such as precise regulation the gelation time mechanical strength within a wide window. Hyperbranched polymers (HBPs), unique highly branched structures abundant functional sites, can be easily prepared functionalized enable decoupled modulation properties address challenges IHs. Our research group developed library HBPs via dynamically controllable polymerization method built series adjustable, controllable, responsive based on resulting HBPs. The fed by demonstrate an adjustable process, wide-range tuning properties, responsiveness demand, which show capabilities various applications. In this review, we summarize role self-healing ability, achieving through extending them broad range are still its infancy. This review provides overview fabricated variety multifunctional HBPs, diverse fields also presented. Meanwhile, point out future development potential challenges.

Language: Английский

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