Published: July 4, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)
Published: June 18, 2024
Since two-dimensionalal (2D) materials have distinct chemical and physical properties, they are widely used in various sectors of modern technologies. In the domain diagnostic biodevices, particularly for point-of-care (PoC) biomedical diagnostics, 2D-based field-effect transistor biosensors (bio-FETs) demonstrate substantial potential. Here, this review article, operational mechanisms detection capabilities biosensing devices utilizing graphene, transition metal dichalcogenides (TMDCs), black phosphorus, other 2D addressed detail. The incorporation these into FET-based offers significant advantages, including low limits (LOD), real-time monitoring, label-free diagnosis, exceptional selectivity. also highlights diverse applications biosensors, ranging from conventional to wearable devices, underscoring versatility material-based FET devices. Additionally, provides a comprehensive assessment limitations challenges faced by along with insights future prospects advancements. Notably, detailed comparison is tabulated platforms their working mechanisms. Ultimately, aims stimulate further research innovation field while educating scientific community about latest advancements materials-based biosensors.
Language: Английский
Citations
17
ACS Sensors, Journal Year: 2024, Volume and Issue: 9(6), P. 2705 - 2727
Published: June 6, 2024
The ultrasensitive recognition of biomarkers plays a crucial role in the precise diagnosis diseases. Graphene-based field-effect transistors (GFET) are considered most promising devices among next generation biosensors. GFET biosensors possess distinct advantages, including label-free, ease integration and operation, ability to directly detect liquid environments. This review summarized recent advances for biomarker detection, with focus on interface functionalization. Various sensitivity-enhancing strategies have been overviewed biosensors, from perspective optimizing graphene synthesis transfer methods, refinement surface functionalization channel layer gate electrode, design biorecognition elements reduction nonspecific adsorption. Further, this extensively explores functionalized antibodies, aptamers, enzymes. It delves into employed detection various diseases (such as cancer, cardiovascular diseases, neurodegenerative disorders, infectious viruses, etc.) along their application integrated microfluidic systems. Finally, issues challenges modulation biosensing interfaces faced by detecting biomarkers.
Language: Английский
Citations
12
Discover Nano, Journal Year: 2024, Volume and Issue: 19(1)
Published: June 17, 2024
Abstract Graphene-based nanomaterials (graphene, graphene oxide, reduced quantum dots, graphene-based nanocomposites, etc.) are emerging as an extremely important class of primarily because their unique and advantageous physical, chemical, biological, optoelectronic aspects. These features have resulted in uses across diverse areas scientific research. Among all other applications, they found to be particularly useful designing highly sensitive biosensors. Numerous studies established efficacy sensing pathogens biomolecules allowing for the rapid diagnosis various diseases. Considering growing importance popularity materials biosensing this review aims provide readers with a summary recent progress concerned domain highlights challenges associated synthesis application these multifunctional materials.
Language: Английский
Citations
8
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117092 - 117092
Published: Dec. 1, 2024
Language: Английский
Citations
7
Talanta, Journal Year: 2024, Volume and Issue: 279, P. 126604 - 126604
Published: July 22, 2024
Language: Английский
Citations
4
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 5, 2025
We designed, developed, and clinically tested two rapid antigen-based immunosensors for SARS-CoV-2 detection, enabling diagnosis viral load quantification under USD $2. In a first clinical study, screen-printed disposable carbon-based (SPC) sensor was assessed on prospectively recruited adult participants classified into three study groups: healthy donors (n = 46); SARS-CoV-2-infected symptomatic patients 58); co-habitants of without prior testing 38). Nasopharyngeal aspirates (NA), oropharyngeal swabs (OS), saliva (SA) samples were obtained from all participants. Performance measured in terms sensitivity specificity against reference diagnostic RT-qPCR kit analytical (limit LoD) using recombinant material lab tests. A second performed the same design, albeit with laser-induced graphene (LIG) electrodes, nasopharyngeal (NS) 224 patient at different stages pandemic, which 110 negative 114 positive via RT-qPCR. find OS most informative sample, when compared to NA SA. The SPC-based sensors had 93.8% 61.5% samples, while LIG-based NS lower 68.93%, significantly higher 86.17%. believe values SPC driven by results affected low (75.5%) high LoD (> 20,000 copies/mL) used, due reduced set effective antigen-binding sites caused non-covalent LIG-mAb ligands used. immunosensor's spike protein phosphate-buffered saline (PBS) both types near 1 fg/mL showed no cross-reactivity structural proteins Epstein-Barr Influenza. metrics time-to-result (5 < 12 min) provide proof-of-principle applicability as low-cost, technology determining infections. Changing working electrode LIG, instead SPC, improved even presence pathogen variants. Discordant between our immunosensor versions tests are attributed not only limited antibody effectiveness former but also quality probes used height pandemic.
Language: Английский
Citations
0
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Abstract Biological Field Effect Transistors (Bio‐FETs) are redefining the standard of biosensing by enabling label‐free, real‐time, and extremely sensitive detection biomolecules. At center this innovation is fundamental empowering role advanced materials, such as graphene, molybdenum disulfide, carbon nanotubes, silicon. These when harnessed with downstream biomolecular probes like aptamers, antibodies, enzymes, allow Bio‐FETs to offer unrivaled sensitivity precision. This review an exposition how advancements in materials science have permitted detect biomarkers low concentrations, from femtomolar attomolar levels, ensuring device stability reliability. Specifically, examines incorporation cutting‐edge architectures, flexible / stretchable multiplexed designs, expanding frontiers contributing development more adaptable user‐friendly Bio‐FET platforms. A key focus placed on synergy artificial intelligence (AI), Internet Things (IoT), sustainable approaches fast‐tracking toward transition research into practical healthcare applications. The also explores current challenges material reproducibility, operational durability, cost‐effectiveness. It outlines targeted strategies address these hurdles facilitate scalable manufacturing. By emphasizing transformative played their cementing position Bio‐FETs, positions a cornerstone technology for future solution precision would lead era where herald massive strides biomedical diagnostics subsume.
Language: Английский
Citations
0
Langmuir, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 5, 2025
The detection of carbohydrate antigen 19–9 (CA199), a critical biomarker for pancreatic, colorectal, and gastric cancers, is essential early diagnosis disease monitoring. Traditional antibody-based assays CA199 are limited by high costs, time-consuming procedures, susceptibility to nonspecific interference. This study presents an electrochemical biosensor based on multifunctional poly(EDOT-PyBA-co-EDOT-PEG) copolymer designed overcome these limitations. integrates pyridylboronic acid (PyBA) moieties specific recognition sialic residues poly(ethylene glycol) (PEG) chains antifouling properties within conductive PEDOT framework. By eliminating antibody dependency, the reduces enhances stability, simplifies diagnostic process. Surface characterization confirmed successful incorporation PyBA PEG units, while impedance spectroscopy enabled sensitive with limit 0.05 U·mL–1 linear response range from 40 U·mL–1. Recovery tests in spiked human serum samples demonstrated excellent accuracy (97–109% recovery), validating biosensor's reliability complex biological matrices. rationally provides both sensitivity specificity maintaining resistance biofouling samples. work establishes scalable, cost-effective platform glycoprotein detection, advancing field clinical diagnostics cancer
Language: Английский
Citations
0
Small, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
Electrochemical transistor (ECT) biosensors can achieve ultrahigh sensitivity, while keeping challenges for large biomolecules due to their sizes over the thickness of electric double layer (EDL) causing a failure sensing reaction. Here, wafer-level Ni/Fe-metal-organic framework (MOF) films and corresponding ECT are fabricated detection coronavirus nucleocapsid protein, which realize an ultralow limit 1 fg mL-1 wide range 100 pg mL-1. A 3D Debye space, originating from unique stacking structure nanosheets nanoparticles in Ni/Fe-MOF film, is proposed explain such ensures immune-binding process protein molecules located effective EDL space. Moreover, appropriate Ni/Fe molar ratio optimized enhance transmission carriers, thus improving signal-to-noise response. The Ni/Fe-MOF-ECT will find applications highly sensitive biomolecules, space may be design model constructing other biosensors.
Language: Английский
Citations
0
Advanced Sensor Research, Journal Year: 2025, Volume and Issue: unknown
Published: May 8, 2025
Abstract The COVID‐19 crisis has defined the need of novel approaches for a straightforward management pandemic and epidemic conditions. Here, an approach based on electronic sensing viral RNA is proposed both virus detection variant tracking. strategy Organic Charge‐Modulated Field‐Effect Transistor (OCMFET) employed development multi‐sensing platform, capable parallel evaluation control references investigated samples. PCR‐free genomic demonstrated at different target concentrations, with optimal discrimination respect to negative control. Moreover, same feasible tracking, as screening mutation in PCR‐amplified samples: can be used rationalization sequencing procedures, which are difficult perform acute phases pandemics, but fundamental effective management. results pave way strategies enhance cost‐effectiveness, easiness‐of‐use combination selectivity sensitivity during severe conditions, more tracking by optimizing access resources even pandemic.
Language: Английский
Citations
0