Assessment of Fallopian Tube Epithelium Features Derived from Induced Pluripotent Stem Cells of Both Fallopian Tube and Skin Origins DOI Creative Commons

Yu‐Hsun Chang,

Kun‐Chi Wu, Kai‐Hung Wang

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(22), P. 2635 - 2635

Published: Nov. 16, 2023

Fallopian tube epithelial cells (FTECs) play a significant role in the development of high-grade serous ovarian cancer (HGSOC), but their utilization vitro experiments presents challenges. To address these limitations, induced pluripotent stem (iPSCs) have been employed as potential solution, driven by hypothesis that orthologous iPSCs may offer superior differentiation capabilities compared with non-orthologous counterparts. Our objective was to generate from FTECs, referred FTEC-iPSCs, and compare derived skin keratinocytes (NHEK). By introducing four-factor Sendai virus transduction system, we successfully FTECs. assess capacity iPSCs, utilized embryoid body formation, revealing positive immunohistochemical staining for markers representing three germ layers. In vivo tumorigenesis evaluation further validated pluripotency evidenced formation tumors immunodeficient mice, histological analysis confirming presence tissues all Quantitative polymerase chain reaction (qPCR) illuminated sequential shift gene expression, encompassing pluripotent, mesodermal, intermediate mesoderm-related genes, during iPSC process into Notably, introduction WNT3A following mesoderm steered toward FTEC phenotype, supported expression FTEC-related tubule-like structures. specific culture conditions, genes comparable FTECs FTEC-iPSCs those NHEK-iPSCs. conclude, our study generated demonstrating differentiation. Furthermore, originating cell sources exhibited capabilities. These findings hold promise using modeling investigating diseases associated types.

Language: Английский

Landscape of human organoids: Ideal model in clinics and research DOI Creative Commons
Xinxin Han, Chunhui Cai, Wei Deng

et al.

The Innovation, Journal Year: 2024, Volume and Issue: 5(3), P. 100620 - 100620

Published: April 1, 2024

In the last decade, organoid research has entered a golden era, signifying pivotal shift in biomedical landscape. The year 2023 marked milestone with publication of thousands papers this arena, reflecting exponential growth. However, amid burgeoning expansion, comprehensive and accurate overview field been conspicuously absent. Our review is intended to bridge gap, providing panoramic view rapidly evolving We meticulously analyze from eight distinctive vantage points, harnessing our rich experience academic research, industrial application, clinical practice. present deep exploration advances technology, underpinned by long-standing involvement arena. narrative traverses historical genesis organoids their transformative impact across various sectors, including oncology, toxicology, drug development. delve into synergy between avant-garde technologies such as synthetic biology single-cell omics discuss role tailoring personalized medicine, enhancing high-throughput screening, constructing physiologically pertinent disease models. analysis reflective discourse provide dive existing landscape emerging trends technology. spotlight technological innovations, methodological evolution, broadening spectrum applications, emphasizing revolutionary influence discovery, other fields. Looking ahead, we cautiously anticipate future developments especially its potential implications for patient care, new avenues research. trust that will be an asset researchers, clinicians, patients keen interest medical strategies. offer broad prospective capabilities encompassing wide range current applications. summary, attempt field. reflections, summaries, projections might useful researchers hope contribute shaping trajectory dynamic advancing

Language: Английский

Citations

34
Patient-derived organoids in human cancer: a platform for fundamental research and precision medicine DOI Creative Commons
Shanqiang Qu, Rongyang Xu,

Guozhong Yi

et al.

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Feb. 12, 2024

Abstract Cancer is associated with a high degree of heterogeneity, encompassing both inter- and intra-tumor along considerable variability in clinical response to common treatments across patients. Conventional models for tumor research, such as vitro cell cultures vivo animal models, demonstrate significant limitations that fall short satisfying the research requisites. Patient-derived organoids, which recapitulate structures, specific functions, molecular characteristics, genomics alterations expression profiles primary tumors. They have been efficaciously implemented illness portrayal, mechanism exploration, high-throughput drug screening assessment, discovery innovative therapeutic targets potential compounds, customized treatment regimen cancer In contrast conventional organoids offer an intuitive, dependable, efficient model by conserving phenotypic, genetic diversity, mutational attributes originating tumor. Nevertheless, organoid technology also confronts bottlenecks challenges, how comprehensively reflect microenvironment, angiogenesis, reduce costs, establish standardized construction processes while retaining reliability. This review extensively examines use techniques fundamental precision medicine. It emphasizes importance patient-derived biobanks development, screening, safety evaluation, personalized Additionally, it evaluates application experimental better understand mechanisms The intent this explicate significance present new avenues future research.

Language: Английский

Citations

18
Radiopharmaceuticals and their applications in medicine DOI Creative Commons
Siqi Zhang, Xingkai Wang, Xin Gao

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: Jan. 2, 2025

Abstract Radiopharmaceuticals involve the local delivery of radionuclides to targeted lesions for diagnosis and treatment multiple diseases. Radiopharmaceutical therapy, which directly causes systematic irreparable damage cells, has attracted increasing attention in refractory diseases that are not sensitive current therapies. As Food Drug Administration (FDA) approvals [ 177 Lu]Lu-DOTA-TATE, Lu]Lu-PSMA-617 their complementary diagnostic agents, namely, 68 Ga]Ga-DOTA-TATE Ga]Ga-PSMA-11, radiopharmaceutical-based theranostics (radiotheranostics) being increasingly implemented clinical practice oncology, lead a new era radiopharmaceuticals. The generation radiopharmaceuticals utilizes targeting vector achieve accurate avoid off-target deposition, making it possible improve efficiency biosafety tumour therapy. Numerous studies have focused on developing novel broader range disease targets, demonstrating remarkable vivo performance. These include high tumor uptake, prolonged retention time, favorable pharmacokinetic properties align with standards. While radiotheranostics been widely applied applications now expanding neurodegenerative diseases, cardiovascular inflammation. Furthermore, radiotheranostic-empowered precision medicine is revolutionizing cancer paradigm. Diagnostic play pivotal role patient stratification planning, leading improved therapeutic outcomes radionuclide This review offers comprehensive overview evolution radiopharmaceuticals, including both FDA-approved clinically investigated explores mechanisms cell death induced by It emphasizes significance future prospects theranostic-based advancing medicine.

Language: Английский

Citations

11
Progress in biomaterials inspired by the extracellular matrix DOI Creative Commons

Zhuolin Chen,

Chengcheng Du, Senrui Liu

et al.

Giant, Journal Year: 2024, Volume and Issue: 19, P. 100323 - 100323

Published: July 10, 2024

Inspired by the extracellular matrix (ECM), biomaterials have emerged as promising strategies in biomedical research and engineering domain, offering unique characteristics for tissue regeneration, drug delivery, therapeutic interventions, cellular investigations. The ECM, a dynamic network structure secreted various cells, primarily comprises diverse proteins capable of facilitating tissue-ECM signaling regulatory functions through its rich array bioactive substances multi-level structural properties. Drawing inspiration from intricate biochemical composition natural researchers developed to encapsulate these features create biomimetic microenvironments, such electrospinning, hydrogels/hydrogel microspheres, decellularized ECM(dECM), ECM-mimicking peptides. Furthermore, mimicking ECM components, ECM-inspired exhibit varying degrees functionalization, including providing support, cell adhesion, signal transduction, mitigating immune responses, remodeling. In summary, advancements offer significant promise addressing key challenges fields engineering, regenerative medicine, delivery.

Language: Английский

Citations

14
From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications DOI

Yisha Huang,

Tong Liu,

Qi Huang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(7), P. 3466 - 3488

Published: July 11, 2024

Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology also facing notable challenges, such as lack vascularization, development multiorgan-on-a-chip systems, replication body single chip. The progress has played crucial role in steering OOC toward mimicking microenvironment, including microenvironment replication, multiorgan microphysiological systems. Additionally, advancements detection, analysis, organoid imaging technologies have enhanced efficiency Organs-on-Chips (OOCs). In particular, integration artificial intelligence revolutionized imaging, significantly enhancing high-throughput drug screening. Consequently, review covers research Human-on-a-chip, sensors OOCs, latest applications biomedical field.

Language: Английский

Citations

12
Stimuli-responsive polymer-based nanosystems for cardiovascular disease theranostics DOI

Yuying Liu,

Congcong Li, Xiao Yang

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(15), P. 3805 - 3825

Published: Jan. 1, 2024

Stimuli-responsive polymer-based nanosystems have gained significant attention for cardiovascular disease theranostics. This review summarizes various stimuli-responsive used diagnosing and treating diseases.

Language: Английский

Citations

6
AI-driven development of high-performance solid-state hydrogen storage DOI Creative Commons
Guoqing Wang,

Zongmin Luo,

Halefom G. Desta

et al.

Energy Reviews, Journal Year: 2024, Volume and Issue: 4(1), P. 100106 - 100106

Published: Aug. 10, 2024

Energy drives the development of human civilization, and hydrogen energy is an inevitable choice under goal "global transition". As technology continues to advance, solid-state storage materials have attracted significant attention as efficient solution for storage. However, existing research methods, such experimental preparation theoretical calculations, are inefficient costly. Here, we summarize latest advancements high-throughput screening (HTS) machine learning (ML) materials. It elaborates on advantages HTS ML in rapid material screening, performance assessment prediction, so on. We place particular emphasis exploration analysis progress involving application various types Additionally, discuss integrating ML, emphasizing this comprehensive strategy field In realm storage, artificial intelligence plays a dual role. not only enhances efficiency but also offers novel tools future design development. This will aid discovery new-type high-performance materials, facilitate their commercialization practical application.

Language: Английский

Citations

6
Bone and Joint‐on‐Chip Platforms: Construction Strategies and Applications DOI
Chengcheng Du, Jiacheng Liu, Senrui Liu

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: May 19, 2024

Organ-on-a-chip, also known as "tissue chip," is an advanced platform based on microfluidic systems for constructing miniature organ models in vitro. They can replicate the complex physiological and pathological responses of human organs. In recent years, development bone joint-on-chip platforms aims to simulate processes occurring bones joints, including cell-cell interactions, interplay various biochemical factors, effects mechanical stimuli, intricate connections between multiple future, will integrate advantages disciplines, bringing more possibilities exploring disease mechanisms, drug screening, personalized medicine. This review explores construction application Organ-on-a-chip technology joint research, proposes a modular concept, discusses new opportunities future challenges platforms.

Language: Английский

Citations

6
Mitochondrial biogenesis microcarriers break membrane barriers for endogenous nucleus pulposus repair DOI
Xiaohu Li, Hongze Chang, Libin Pang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160668 - 160668

Published: Feb. 1, 2025

Citations

0
Tumor Microenvironment‐Responsive Polymer‐Based RNA Delivery Systems for Cancer Treatment DOI

Yahan Zhang,

Ming Zhang,

Haiqin Song

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract Ribonucleic acid (RNA) therapeutics offer a broad prospect in cancer treatment. However, their successful application requires overcoming various physiological barriers to effectively deliver RNAs the target sites. Currently, number of RNA delivery systems based on polymeric nanoparticles are developed overcome these delivery. This work provides an overview existing for gene therapy, and particularly summarizes those that entering clinical phase. then discusses core features latest research developments tumor microenvironment‐responsive polymer‐based carriers which designed pathological characteristics microenvironment. Finally, this also proposes opportunities transformation therapies into immunotherapy methods applications.

Language: Английский

Citations

3