Co‐expression of transcription factors ZmC1 and ZmR2 establishes an efficient and accurate haploid embryo identification system in maize DOI Creative Commons
Chen Chen, Xiaoqing Liu, Suzhen Li

et al.

The Plant Journal, Journal Year: 2022, Volume and Issue: 111(5), P. 1296 - 1307

Published: July 6, 2022

Because of their high efficiency during chromosome doubling, immature haploid maize (Zea mays L.) embryos are useful for doubled production. The R1-nj marker is commonly used in breeding and has improved the identification. However, its effectiveness limited by genetic background environmental factors. We addressed this technical challenge developing an efficient accurate embryo identification through co-expression two transcription factor genes (ZmC1 ZmR2) driven embryo-aleurone-specific bidirectional promoter PZmBD1 ; these factors can activate anthocyanin biosynthesis aleurone layer early seed development. developed a new inducer, Maize Anthocyanin Gene InduCer 1 (MAGIC1), introducing transgenes into inducer line CAU6. MAGIC1 could identify haploids at 12 days after pollination, which nine earlier than Importantly, increased accuracy to 99.1%, compared with 88.3% In addition, effectively overcome inhibition synthesis some germplasms. Furthermore, upgraded was from ZmC1 ZmR2 generate MAGIC2, diploids due differential accumulation embryos, coleoptiles, sheaths, roots, leaves, dry seeds. This system more conventional R1-nj-based method, it simplifies process. Therefore, provides support large-scale

Language: Английский

Seed Science and Technology DOI Creative Commons

Malavika Dadlani,

Devendra Kumar Yadava

Published: Jan. 1, 2023

This open access edited book is a collection of 21 chapters synthesized from nearly 100 lectures by >70 eminent international and Indian experts.

Language: Английский

Citations

831
Beat the stress: breeding for climate resilience in maize for the tropical rainfed environments DOI Creative Commons
B. M. Prasanna, Jill E. Cairns, P.H. Zaidi

et al.

Theoretical and Applied Genetics, Journal Year: 2021, Volume and Issue: 134(6), P. 1729 - 1752

Published: Feb. 16, 2021

Abstract Key message Intensive public sector breeding efforts and public-private partnerships have led to the increase in genetic gains, deployment of elite climate-resilient maize cultivars for stress-prone environments tropics. Maize ( Zea mays L.) plays a critical role ensuring food nutritional security, livelihoods millions resource-constrained smallholders. However, yields tropical rainfed are now increasingly vulnerable various climate-induced stresses, especially drought, heat, waterlogging, salinity, cold, diseases, insect pests, which often come combinations severely impact crops. The International Wheat Improvement Center (CIMMYT), partnership with several private institutions, has been intensively engaged over last four decades germplasm tolerance key abiotic biotic using an extensive managed stress screening network on-farm testing system. This successful development array stress-tolerant across sub-Saharan Africa, Asia, Latin America. Further increasing gains programs demands judicious integration doubled haploidy, high-throughput precise phenotyping, genomics-assisted breeding, data management, more effective decision support tools. Multi-institutional efforts, public–private alliances, ensure that improved varieties effectively reach climate-vulnerable farming communities tropics, including accelerated replacement old/obsolete varieties.

Language: Английский

Citations

162
Plant regeneration in the new era: from molecular mechanisms to biotechnology applications DOI
Chunli Chen, Yuxin Hu, Momoko Ikeuchi

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(7), P. 1338 - 1367

Published: May 31, 2024

Language: Английский

Citations

21
Artificial polyploidy induction for improvement of ornamental and medicinal plants DOI
Mohsen Niazian,

Ayoub Molaahmad Nalousi

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2020, Volume and Issue: 142(3), P. 447 - 469

Published: July 16, 2020

Language: Английский

Citations

86
Genome editing in cereal crops: an overview DOI Creative Commons

Jerlie Mhay Matres,

Julia Hilscher, Akash Datta

et al.

Transgenic Research, Journal Year: 2021, Volume and Issue: 30(4), P. 461 - 498

Published: July 14, 2021

Abstract Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state genome editing in major cereal crops- rice, maize, wheat barley. Genome has been used to achieve important agronomic quality traits cereals. These include adaptive mitigate effects climate change, tolerance biotic stresses, higher yields, more optimal plant architecture, improved grain nutritional content, safer products. Not all can be achieved through editing, several technical regulatory challenges need overcome technology realize its full potential. however, already revolutionized is poised shape future agricultural practices conjunction other breeding innovations.

Language: Английский

Citations

62
BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize DOI Open Access
Christian Damian Lorenzo, Kévin Debray, Denia Herwegh

et al.

The Plant Cell, Journal Year: 2022, Volume and Issue: 35(1), P. 218 - 238

Published: Sept. 6, 2022

Abstract Ensuring food security for an ever-growing global population while adapting to climate change is the main challenge agriculture in 21st century. Although new technologies are being applied tackle this problem, we approaching a plateau crop improvement using conventional breeding. Recent advances CRISPR/Cas9-mediated gene engineering have paved way accelerate plant breeding meet increasing demand. However, many traits governed by multiple small-effect genes operating complex interactive networks. Here, present discovery pipeline BREEDIT, which combines multiplex genome editing of whole families with crossing schemes improve such as yield and drought tolerance. We induced knockouts 48 growth-related into maize (Zea mays) CRISPR/Cas9 generated collection over 1,000 gene-edited plants. The edited populations displayed (on average) 5%–10% increases leaf length up 20% width compared controls. For each family, edits subsets could be associated enhanced traits, allowing us reduce space considered trait improvement. BREEDIT rapidly generate diverse mutants identify promising modifications later use programs.

Language: Английский

Citations

60
Drought stress in sorghum: Mitigation strategies, breeding methods and technologies—A review DOI
Muhammad Ahmad Yahaya, Hussein Shimelis

Journal of Agronomy and Crop Science, Journal Year: 2021, Volume and Issue: 208(2), P. 127 - 142

Published: Nov. 15, 2021

Abstract Agriculture accounts for 70% of the global use available freshwater. Projections show that demand water will increase significantly due to climate change, population growth and development agricultural enterprises globally. There is a need develop water‐use efficient crop cultivars sustainable production. Sorghum [ bicolor (L.) Moench.] powerhouse in drier regions supporting more than 500 million people. It relatively drought‐tolerant adapted grow yield marginal environments where other dominant crops such as maize wheat fail survive. However, mean sorghum semi‐arid has stagnated around 1.0 ton/ha compared with average 2.5 ton/ha, mainly recurrent droughts heat stress. Breeding an economic mitigation strategy against current projected drought Therefore, objectives this review were document impact stress key strategies under drought‐prone production systems. The first section highlighted its emphasizing on best strategy. This followed by perspectives aspects drought‐response mechanisms, breeding methods complementary technologies tolerance. Integration conventional molecular rapid generation advancement could reduce cycle efficiency deploying new varieties. Information presented guide agronomists breeders deploy are changing regions.

Language: Английский

Citations

58
Combining speed breeding with traditional and genomics‐assisted breeding for crop improvement DOI
Saurabh Pandey, Ashutosh Singh, Swarup K. Parida

et al.

Plant Breeding, Journal Year: 2022, Volume and Issue: 141(3), P. 301 - 313

Published: March 30, 2022

Abstract Accelerated crop growth strategy innovations are required as we reach saturation peaks regarding the productivity of major food crops. Speed breeding (SB) is one most promising technologies adopted for this purpose. SB hastens production by reducing plant and development, time swift generation advancement. Prolonged daily light exposure shortens life cycle in some long‐day or day‐neutral plants leading to early seed harvest. This approach best suited controlled environment prebreeding/breeding activities analysed several species. can be integrated with different traditional advanced genomics‐assisted like marker‐assisted selection (MAS), genomic (GS), pollen‐based (PBS), overexpression/knock‐down transgenics genome editing achieve more precise faster results on translational genetic enhancement. review will discuss approaches strategies its potential integrate existing improvement attain efficient outcomes crops' varietal improvement.

Language: Английский

Citations

46
The RUBY reporter enables efficient haploid identification in maize and tomato DOI Creative Commons
Dong Wang, Yu Zhong, Bin Feng

et al.

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21(8), P. 1707 - 1715

Published: May 17, 2023

Summary In vivo haploid induction has been extended from maize to monocotyledonous plants like rice, wheat, millet and dicotyledonous such as tomato, rapeseed, tobacco cabbage. Accurate identification of haploids is a crucial step doubled technology, where useful marker very pivotal. R1‐nj an extensively used visual for in maize. RFP eGFP have shown be feasible identifying haploid. However, these methods are either limited specific species, or require equipment. It still lacks efficient that practical across different crop species. this study, we introduced the RUBY reporter, betalain biosynthesis system, into tomato inducers new identification. Results showed expression could result deep pigmentation embryos early 10 days after pollination, enabled 100% accuracy immature embryo Further investigation revealed led red radicles can identified easily accurately. The results demonstrated reporter background‐independent would promising breeding

Language: Английский

Citations

37
Embryo Rescue in Plant Breeding DOI Creative Commons
Ugo Rogo, Marco Fambrini, Claudio Pugliesi

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(17), P. 3106 - 3106

Published: Aug. 29, 2023

Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote development an immature or lethal embryo into viable plant. Intraspecific, interspecific, intergeneric crosses allow introgression important alleles agricultural interest from wild species, such as resistance tolerance abiotic biotic stresses morphological traits crops. However, pre-zygotic post-zygotic reproductive barriers often present challenges achieving hybridization. Pre-zygotic manifest incompatibility reactions hinder pollen germination, tube growth, penetration ovule occurring various tissues, stigma, style, ovary. To overcome these barriers, several strategies employed, including cut-style graft-on-style techniques, utilization mixed distinct placenta pollination, pollination. On other hand, act at different tissues stages ranging early subsequent growth reproduction offspring. Many genera result abortion due failure endosperm development. In cases, needed hybrids. holds great promise for not only facilitating but also obtaining haploids, doubled manipulating ploidy levels chromosome engineering by monosomic disomic addition well substitution lines. Furthermore, can be shorten cycle propagation rare plants. Additionally, it has been repeatedly study embryonic development, especially embryo-lethal mutants. The widely procedure is embryos taken placed directly on media. certain ovule, ovaries placentas enables young zygote stage maturity.

Language: Английский

Citations

24