Low dose dietary contamination with deoxynivalenol mycotoxin exacerbates enteritis and colorectal cancer in mice DOI Creative Commons
Madjid Djouina, Christophe Waxin, Ségolène Caboche

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 900, P. 165722 - 165722

Published: July 22, 2023

The mycotoxin deoxynivalenol (DON) is a frequent contaminant of grain and cereal products worldwide. Exposure to DON can cause gastrointestinal inflammation, disturb gut barrier function, induce dysbiosis in vivo under basal conditions, but little known about the effects ingestion individuals with pre-existing disease.Mice were orally exposed 10 100 μg/kg bw/day DON, corresponding 100-fold human tolerable daily intake concentrations, translation mice current intake. exposure explored steady-state murine models enteritis colorectal cancer (CRC).After 8 days exposure, an increase histomorphological molecular parameters epithelial proliferation observed normal mice, from duodenum colon. same model indomethacin-induced led exacerbation lesion development induction ileal cytokines. also worsened colitis-associated CRC as shown by increases endoscopic histological colitis scores, tumor grades, hyperplasia. In colon DON-exposed upstream downstream ERK signaling genes upregulated including Mapk1, Mapk3, Map 2k1, Map2k2 core pathway effectors, Bcl2 Bcl2l1 antiapoptotic genes. associated alterations cecal microbiota taxonomic composition metabolism bacterial fucose rhamnose. Strong Spearman's correlations revealed between relative abundance changed genera CRC-related variables.Ingestion at concentrations representative real-world mice. Our results suggest that even low doses, which are currently tolerated diet, could promote intestinal inflammatory diseases CRC.

Language: Английский

An update on immunotoxicity and mechanisms of action of six environmental mycotoxins DOI
Yuhang Sun, Kehe Huang, Miao Long

et al.

Food and Chemical Toxicology, Journal Year: 2022, Volume and Issue: 163, P. 112895 - 112895

Published: Feb. 25, 2022

Language: Английский

Citations

76
Role of hypoxia in cellular senescence DOI Creative Commons
Haoyu Gao, Eugenie Nepovimová, Zbyněk Heger

et al.

Pharmacological Research, Journal Year: 2023, Volume and Issue: 194, P. 106841 - 106841

Published: June 28, 2023

Senescent cells persist and continuously secrete proinflammatory tissue-remodeling molecules that poison surrounding cells, leading to various age-related diseases, including diabetes, atherosclerosis, Alzheimer's disease. The underlying mechanism of cellular senescence has not yet been fully explored. Emerging evidence indicates hypoxia is involved in the regulation senescence. Hypoxia-inducible factor (HIF)- 1α accumulates under hypoxic conditions regulates by modulating levels markers p16, p53, lamin B1, cyclin D1. Hypoxia a critical condition for maintaining tumor immune evasion, which promoted driving expression genetic factors (such as p53 CD47) while triggering immunosenescence. Under conditions, autophagy activated targeting BCL-2/adenovirus E1B 19-kDa interacting protein 3, subsequently induces p21WAF1/CIP1 well p16Ink4a increases β-galactosidase (β-gal) activity, thereby inducing Deletion p21 gene activity response regulator poly (ADP-ribose) polymerase-1 (PARP-1) level nonhomologous end joining (NHEJ) proteins, repairs DNA double-strand breaks, alleviates Moreover, associated with intestinal dysbiosis an accumulation D-galactose derived from gut microbiota. Chronic leads striking reduction amount Lactobacillus D-galactose-degrading enzymes gut, producing excess reactive oxygen species (ROS) bone marrow mesenchymal stem cells. Exosomal microRNAs (miRNAs) long noncoding RNAs (lncRNAs) play important roles miR-424-5p are decreased hypoxia, whereas lncRNA-MALAT1 increased, both induce present review focuses on recent advances understanding role effects HIFs, PARP-1, microbiota, exosomal mRNA hypoxia-mediated cell specifically discussed. This our provides new clues anti-aging processes treatment aging-related diseases.

Language: Английский

Citations

56
Review of neurotoxicity of T-2 toxin DOI

Youshuang Wang,

Bo Wang, Peilin Wang

et al.

Mycotoxin Research, Journal Year: 2024, Volume and Issue: 40(1), P. 85 - 95

Published: Jan. 13, 2024

Language: Английский

Citations

11
Toxic mechanisms of the trichothecenes T-2 toxin and deoxynivalenol on protein synthesis DOI

Jiefeng Li,

Yating Wang, Ying Deng

et al.

Food and Chemical Toxicology, Journal Year: 2022, Volume and Issue: 164, P. 113044 - 113044

Published: April 19, 2022

Language: Английский

Citations

37
Double-Enzyme Active Vanadium Nanospheres-Mediated Ratiometric Multicolor Immunosensors for Sensitive Detection of the T-2 Toxin DOI

Haiyu Wu,

Tong Bu, Yuanyuan Cao

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(12), P. 5275 - 5284

Published: March 10, 2023

Owing to its high throughput, simplicity, and rapidity, enzyme-linked immunosorbent assay (ELISA) has attracted much attention in the field of immunoassays. However, traditional ELISA usually affords a single signal readout labeling ability enzyme used is poor, resulting low accuracy limited detection range. Herein, vanadium nanospheres (VNSs)-mediated competitive ratio nanozymes-linked (VNSs-RNLISA) was created for sensitive T-2 toxin (T-2). As key biosensor, VNSs with superoxide dismutase-like peroxidase-like dual-enzyme mimetic activities were synthesized by one-step hydrothermal method, which oxidized 1,1-diphenyl-2-picryl-hydrazyl fading catalyzed 3,3',5,5'-tetramethylbenzidine (TMB) color development. Therefore, could not only be qualitatively measured naked eye but also quantitatively evaluated monitoring absorbance at 450 517 nm wavelengths. Moreover, characterization VNSs-labeled antibody probe showed strong dual-enzymatic activity, excellent stability, affinity [the constant (ka) approximately 1.36 × 108 M-1], can significantly improve sensitivity. The limit VNSs-RNLISA 0.021 ng/mL, 27-fold more than (0.561 ng/mL). Besides, change (Δ450/Δ517) decreased linearly range 0.22-13.17 outperforming single-mode nano-enzyme-linked using TMB factor 1.6 times. Furthermore, successfully identify maize oat samples, recoveries ranging from 84.216 125.371%. Overall, this tactic offered promising platform quick food might broaden application assay.

Language: Английский

Citations

20
Trimethyltin induces apoptosis and necroptosis of mouse liver by oxidative stress through YAP phosphorylation DOI Creative Commons
Yuqi Wang, Xiaojing Liu, Hongyuan Jing

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2022, Volume and Issue: 248, P. 114327 - 114327

Published: Nov. 24, 2022

Trimethyltin (TMT) is widely used as a major component of plastic stabilizers in agriculture and industry, can accumulate large quantities the liver. To investigate relationship between liver tissue damage induced by TMT exposure YAP phosphorylation mice, we gave mice drinking water containing 0.01 mg/mL for 14 days to establish an vivo experimental model, continuously treated AML12 cells with 20 μM 24 h vitro model. Transcriptomics revealed that altered 62,466 apparently diversely expressed genes, including 1197 upregulated 899 downregulated enrichment Hippo pathway occurred. Moreover, western blotting (WB) quantitative real-time PCR (qRTPCR) results showed triggered increase expression P-YAP, apoptosis necroptosis-interrelated decrease Bcl-2 mouse livers tissues cells. The P-YAP was significantly suppressed TRULI-treated TMT-exposed cells, while oxidative stress levels were also attenuated. In conclusion, triggers induce inducing necroptosis tissues. Our confirm toxic effect specific mechanism TMT.

Language: Английский

Citations

27
Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens DOI Creative Commons
Weiwei Wang,

Jingqiang Zhu,

Qingyun Cao

et al.

Toxins, Journal Year: 2022, Volume and Issue: 14(12), P. 830 - 830

Published: Nov. 28, 2022

Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental on antioxidant property and gut microbiota in DON-exposed broilers. A total 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, + (DONC) group. The control group received a diet without with contamination, respectively, while DONC DON-contaminated 200 U/kg added. Parameter analysis was performed d 21. results showed that DON-induced liver enlargement (p < 0.05) blocked by addition, which also normalized increases hepatic metabolites contents caspase-9 expression. Additionally, addition increased jejunal GSH-Px activities coupled T-AOC broilers, as well reductions villus height (VH) its ratio for crypt depth. There difference among groups. enriched some harmful bacteria (e.g., Proteobacteria, Gammaproteobacteria, Enterobacteriales, Enterobacteriaceae, Escherichia/Shigella) elicited negative correlations activity, VH. differentially certain beneficial Acidobacteriota, Anaerofustis, Anaerotruncus) could benefit intestinal antioxidation morphology. In conclusion, alleviates stress damage be associated ability improve microbiota, aside from direct radical-scavenging activity.

Language: Английский

Citations

25
Fusarium mycotoxins: The major food contaminants DOI Creative Commons
Zheng Qu,

Xianfeng Ren,

Zhaolin Du

et al.

mLife, Journal Year: 2024, Volume and Issue: 3(2), P. 176 - 206

Published: May 13, 2024

Mycotoxins, which are secondary metabolites produced by toxicogenic fungi, natural food toxins that cause acute and chronic adverse reactions in humans animals. The genus

Language: Английский

Citations

6
Uridine diphosphate glucuronosyltransferase 1A1 prevents the progression of liver injury DOI Creative Commons

Jin-Lian Jiang,

Yi‐Yang Zhou,

Weiwei Zhong

et al.

World Journal of Gastroenterology, Journal Year: 2024, Volume and Issue: 30(9), P. 1189 - 1212

Published: March 5, 2024

BACKGROUND Uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) plays a crucial role in metabolizing and detoxifying endogenous exogenous substances. However, its contribution to the progression of liver damage remains unclear. AIM To determine mechanism UGT1A1 progression. METHODS We investigated relationship between expression injury through clinical research. Additionally, impact on was analyzed mouse model study. RESULTS Patients with gene mutations showed varying degrees damage, while patients acute-on-chronic failure (ACLF) exhibited relatively reduced levels protein as compared chronic hepatitis. This suggests that low may be associated damage. In models induced by carbon tetrachloride (CCl4) concanavalin A (ConA), hepatic were found increased. mice lipopolysaccharide or steatosis-mediated liver-injury progression, decreased, which is consistent observations ACLF. knockout exacerbated CCl4- ConA-induced injury, hepatocyte apoptosis necroptosis mice, intensified endoplasmic reticulum (ER) stress oxidative stress, disrupted lipid metabolism. CONCLUSION upregulated compensatory response during interference this upregulation process worsen injury. reduces ER metabolism disorder, thereby mitigating necroptosis.

Language: Английский

Citations

5
Mycotoxins and cellular senescence: the impact of oxidative stress, hypoxia, and immunosuppression DOI
You Li, Eugenie Nepovimová, Marián Valko

et al.

Archives of Toxicology, Journal Year: 2022, Volume and Issue: 97(2), P. 393 - 404

Published: Nov. 25, 2022

Language: Английский

Citations

20