Advances in Nanotechnology for Biofilm Inhibition

ACS Omega, Journal Year: 2023, Volume and Issue: 8(24), P. 21391 - 21409

Published: June 7, 2023

Biofilm-associated infections have emerged as a significant public health challenge due to their persistent nature and increased resistance conventional treatment methods. The indiscriminate usage of antibiotics has made us susceptible range multidrug-resistant pathogens. These pathogens show reduced susceptibility intracellular survival. However, current methods for treating biofilms, such smart materials targeted drug delivery systems, not been found effective in preventing biofilm formation. To address this challenge, nanotechnology provided innovative solutions formation by clinically relevant Recent advances nanotechnological strategies, including metallic nanoparticles, functionalized dendrimers, polymeric cyclodextrin-based delivery, solid lipid polymer conjugates, liposomes, may provide valuable technological against infectious diseases. Therefore, it is imperative conduct comprehensive review summarize the recent advancements limitations advanced nanotechnologies. present Review encompasses summary agents, mechanisms that lead formation, impact on human health. In nutshell, offers survey managing infections. A detailed presentation how these strategies improve control prevent key objective mechanisms, applications, prospects nanotechnologies better understanding

Language: Английский

---

Discovery of psoralen as a quorum sensing inhibitor suppresses Pseudomonas aeruginosa virulence

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Feb. 19, 2024

Abstract P seud omon as aeruginosa is a common opportunistic pathogen with growing resistance and presents heightened treatment challenges. Quorum sensing (QS) cell-to-cell communication system that contributes to the production of variety virulence factors also related biofilm formation P. . Compared traditional antibiotics which kill bacteria directly, anti-virulence strategy by targeting QS promising for combating pseudomonal infections. In this study, inhibition potential compounds derived from Traditional Chinese Medicines was evaluated using in silico, vitro, vivo analyses. The results showed psoralen, natural furocoumarin compound Psoralea corylifolia L., capable simultaneously inhibiting three main regulators, LasR, RhlR, PqsR aeruginosa. Psoralen had no bactericidal activity but could widely inhibit extracellular proteases, pyocyanin, biofilm, cell motilities model clinical strains. RNA-sequencing quantitative PCR analyses further demonstrated majority QS-activated genes were suppressed psoralen. supplementation psoralen protect Caenorhabditis elegans challenge, especially hypervirulent strain PA14. Moreover, synergistic antibacterial effects polymyxin B, levofloxacin, kanamycin. conclusions, study identifies anti-QS antibiofilm against strains sheds light on discovery anti-pseudomonal drugs among Medicines. Key points • L. inhibits virulence-related phenotypes targets core regulators expression large part downstream genes. protects C. challenge enhances susceptibility antibiotics.

Language: Английский

---

3D bioprinted poly(lactic acid) scaffolds infused with curcumin-loaded nanostructured lipid carriers: a promising approach for skin regeneration

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

3D bioprinted PLA scaffolds with curcumin-loaded lipid carriers show antimicrobial activity and promote skin regeneration, offering promise for wound healing.

Language: Английский

---

Repurposing phytochemicals as anti‐virulent agents to attenuate quorum sensing‐regulated virulence factors and biofilm formation inPseudomonas aeruginosa

Microbial Biotechnology, Journal Year: 2021, Volume and Issue: 15(6), P. 1695 - 1718

Published: Nov. 29, 2021

Summary Unregulated consumption and overexploitation of antibiotics have paved the way for emergence antibiotic‐resistant strains ‘superbugs’. Pseudomonas aeruginosa is among opportunistic nosocomial pathogens causing devastating infections in clinical set‐ups globally. Its artillery equipped with diversified virulence elements, extensive antibiotic resistance biofilms has made it a ‘hard‐to‐treat’ pathogen. The pathogenicity P. modulated by an intricate cell density‐dependent mechanism called quorum sensing (QS). firmly controlled QS genes, their expression drives aggressiveness infection. Attempts to identify develop novel antimicrobials seen sharp rise past decade. Among different proposed mechanisms, anti‐virulence approach target pseudomonal virtue anti‐QS anti‐biofilm drugs appears occupy centre stage. In this respect, bioactive phytochemicals gained prominence scientific community owing significant quenching (QQ) properties. Recent studies shed light on QQ activities various other perturbing QS‐dependent . This review highlights recent evidences that reinforce application plant bioactives combating infections, advantages shortcomings therapy.

Language: Английский

---

Quercetin: a promising virulence inhibitor of Pseudomonas aeruginosa LasB in vitro

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Jan. 5, 2024

Abstract With the inappropriate use of antibiotics, antibiotic resistance has emerged as a major dilemma for patients infected with Pseudomonas aeruginosa . Elastase B (LasB), crucial extracellular virulence factor secreted by P. , been identified key target antivirulence therapy. Quercetin, natural flavonoid, exhibits promising potential an agent. We aim to evaluate impact quercetin on LasB and elucidate underlying mechanism. Molecular docking molecular dynamics simulation revealed rather favorable intermolecular interaction between LasB. At sub-MICs ≤256 μg/ml, was found effectively inhibit production activity elastase, well downregulate transcription level lasB gene in both PAO1 clinical strains Through correlation analysis, significant positive correlations were shown QS system regulatory genes lasI lasR rhlI rhlR Then, we expression significantly deficient ΔlasI ΔlasIΔrhlI mutants. In addition, downregulated levels regulated rhlI, pqsA pqsR attenuated synthesis signaling molecules 3-oxo-C12-HSL C4-HSL PAO1. Quercetin also able compete ligands OdDHL, BHL, PQS binding receptor proteins LasR, RhlR, PqsR, respectively, resulting formation more stabilized complexes. Taken together, enormous combating disrupting vitro thereby offering alternative approach therapy infections. Key points • diminished content elastase aeruginosa. inhibited acted based system.

Language: Английский

---

A comprehensive review on the pharmacological prospects of Terpinen-4-ol: From nature to medicine and beyond

Fitoterapia, Journal Year: 2024, Volume and Issue: 176, P. 106051 - 106051

Published: June 4, 2024

Language: Английский

---

Gentamicin Augments the Quorum Quenching Potential of Cinnamaldehyde In Vitro and Protects Caenorhabditis elegans From Pseudomonas aeruginosa Infection

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: June 15, 2022

The quorum sensing (QS) circuitry of Pseudomonas aeruginosa represents an attractive target to attenuate bacterial virulence and antibiotic resistance. In this context, phytochemicals harboring anti-virulent properties have emerged as alternative medicine combat pseudomonal infections. Hence, study was undertaken investigate the synergistic effects quenching (QQ) potential cinnamaldehyde (CiNN) in combination with gentamicin (GeN) against P. . QQ activity novel evaluated using a QS reporter strain synergism studied chequerboard assays. Further, genotypic phenotypic expression factors examined alongside biofilm formation. CiNN GeN exhibited synergy promising anti-QS activity. This drug shown suppress AHL production downregulate critical genes PAO1. Molecular docking revealed strong interactions between receptors CiNN, asserting its potential. Bacterial motility compromised along significant reduction pyocyanin (72.3%), alginate (58.7%), rhamnolipid (33.6%), hemolysin (82.6%), protease (70.9%), elastase (63.9%) production. successfully eradicated preformed biofilms inhibited formation by abrogating EPS Our findings suggest that although alone could not QS, but able augment CiNN. To validate our results infection model, we quantified survival rates Caenorhabditis elegans following PAO1 challenge. significantly rescued C. from improved rate 54% at 96 h. summary, is first elucidate mechanism behind prospects (augmented presence GeN) increasing , thereby highlighting properties.

Language: Английский

---

Prevalence of Quorum Sensing and Virulence Factor Genes Among Pseudomonas aeruginosa Isolated from Patients Suffering from Different Infections and Their Association with Antimicrobial Resistance

Infection and Drug Resistance, Journal Year: 2023, Volume and Issue: Volume 16, P. 2371 - 2385

Published: April 1, 2023

Antimicrobial resistance and virulence genes play important roles in increasing the severity of Pseudomonas aeruginosa infections, especially hospitalized patients with high antibiotic pressure. Most that encode factors are controlled regulated by quorum sensing (QS) system. The aim this study was to investigate frequency some (rhlR, rhlI, lasR, lasI, lasB, toxA, aprA, algD, ExoS, plcH genes) their association resistance.Antimicrobial susceptibility determined Kirby-Bauer agar disk diffusion method. A total 125 clinical isolates P. were tested for using polymerase chain reaction (PCR).The highest observed against cefepime (92.8%). Multi-drug resistant (MDR) represented 63.2% distribution among wound (21/79, 26.3% MDR isolates). LasB most prevalent gene (89.6%) followed aprA (85.6%), exoS (84%), algD (80%), toxA (76.8%), (75.2). Furthermore, a significant (P < 0.05) found. presence more than 5 highly otitis media, respiratory tract infection isolates.The complex including QS system regulating indicates importance progression which is considered great challenge health-care team need specific studies each area having different profiles development effective treatment strategies such as anti-virulent inhibiting drugs infections.

Language: Английский

---

Antagonist Impact of Selenium-Based Nanoparticles Against Mycobacterium tuberculosis

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 195(6), P. 3606 - 3614

Published: Jan. 13, 2023

Language: Английский

---

A comprehensive status update on modification of foley catheter to combat catheter-associated urinary tract infections and microbial biofilms

Critical Reviews in Microbiology, Journal Year: 2023, Volume and Issue: 50(2), P. 168 - 195

Published: Jan. 18, 2023

Present-day healthcare employs several types of invasive devices, including urinary catheters, to improve medical wellness, the clinical outcome disease, and quality patient life. Among Foley catheter is most commonly used in patients for bladder drainage collection urine. Although such devices are very useful who cannot empty their various reasons, they also expose catheter-associated tract infections (CAUTIs). Catheter provides an ideal surface bacterial colonization biofilm formation, resulting persistent infection severe complications. Hence, rigorous efforts have been made develop catheters that harbour antimicrobial anti-fouling properties resist by pathogens. In this regard, modification functionalization, impregnation, blending, or coating with antibiotics, bioactive compounds, nanoformulations proved be effective controlling formation. This review attempts illustrate complications associated indwelling primarily focussing on challenges fighting CAUTI, colonization, review, we collate scientific literature using plant components, bacteriophages, nanoparticles, studies demonstrating efficacy through vitro vivo testing.

Language: Английский

---