Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Advanced Healthcare Materials, Journal Year: 2020, Volume and Issue: 9(8)

Published: Feb. 25, 2020

Abstract The vascular system is integral for maintaining organ‐specific functions and homeostasis. Dysregulation in architecture function can lead to various chronic or acute disorders. Investigation of the role health disease has been accelerated through development tissue‐engineered constructs microphysiological on‐chip platforms. These vitro systems permit studies biochemical regulation networks parenchymal tissue provide mechanistic insights into biophysical hemodynamic forces acting niches. Detailed understanding these mechanotransductory pathways involved necessary develop preventative therapeutic strategies targeting system. This review describes structure function, homeostasis, measurement approaches cell level mechanical properties influencing phenomena. State‐of‐the‐art techniques fabricating microvascular systems, with varying degrees biological engineering complexity, are summarized. Finally, mechanobiology niches pathophysiological states, efforts recapitulate events using explored. It hoped that this will help readers appreciate important, but understudied, vascular‐parenchymal mechanotransduction toward developing mechanotherapeutics treatment strategies.

Language: Английский

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Pyroptosis: A New Insight Into Eye Disease Therapy

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 3, 2021

Pyroptosis is a lytic form of programmed cell death mediated by gasdermins (GSDMs) with pore-forming activity in response to certain exogenous and endogenous stimuli. The inflammasomes are intracellular multiprotein complexes consisting pattern recognition receptors, an adaptor protein ASC (apoptosis speck-like protein), caspase-1 cause autocatalytic activation caspase-1, which cleaves gasdermin D (GSDMD), inducing pyroptosis accompanied cytokine release. In recent years, the pathogenic roles multiple eye diseases, including keratitis, dry eyes, cataracts, glaucoma, uveitis, age-related macular degeneration, diabetic retinopathy, have been continuously confirmed. Inhibiting inflammasome abnormal eyes generally attenuates inflammation benefits prognosis. Therefore, insight into pathogenesis underlying development various types diseases may provide new therapeutic strategies for ocular disorders. Inhibitors pyroptosis, such as NLRP3, GSDMD inhibitors, proven be effective many diseases. purpose this article illuminate mechanism emphasize its crucial role addition, we review application modulators

Language: Английский

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IDO1 and inflammatory neovascularization: bringing new blood to tumor-promoting inflammation

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: April 27, 2023

In parallel with the genetic and epigenetic changes that accumulate in tumor cells, chronic tumor-promoting inflammation establishes a local microenvironment fosters development of malignancy. While knowledge specific factors distinguish from non-tumor-promoting remains inchoate, nevertheless, as highlighted this series on ‘Hallmarks Cancer’, it is clear essential to neoplasia metastatic progression making identification critical. Studies immunometabolism inflamometabolism have revealed role for tryptophan catabolizing enzyme IDO1 core element inflammation. At one level, expression promotes immune tolerance antigens, thereby helping tumors evade adaptive control. Additionally, recent findings indicate also neovascularization by subverting innate immunity. This newly recognized function mediated unique myeloid cell population termed IDVCs (IDO1-dependent vascularizing cells). Initially identified lesions, may exert broader effects pathologic various disease settings. Mechanistically, induction inflammatory cytokine IFNγ blocks antagonistic effect stimulating IL6, powerful pro-angiogenic cytokine. By contributing vascular access, ascribed aligns its involvement other cancer hallmark functionalities, (tumor-promoting inflammation, escape, altered cellular metabolism, metastasis), which stem an underlying normal physiological functions such wound healing pregnancy. Understanding nuances how these functionalities varies between different settings will be crucial future successful IDO1-directed therapies.

Language: Английский

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Circulating immune cell phenotyping is potentially relevant for diabetic retinopathy risk assessment

Diabetes Research and Clinical Practice, Journal Year: 2024, Volume and Issue: 211, P. 111667 - 111667

Published: April 10, 2024

Language: Английский

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Advancing Ophthalmic Delivery of Flurbiprofen via Synergistic Chiral Resolution and Ion-pairing Strategies

Asian Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 19(3), P. 100928 - 100928

Published: May 22, 2024

Flurbiprofen (FB), a nonsteroidal anti-inflammatory drug, is widely employed in treating ocular inflammation owing to its remarkable effects. However, the racemic nature of commercially available formulation (Ocufen®) limits full potential therapeutic activity, as (

Language: Английский

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