Horticulturae,
Journal Year:
2023,
Volume and Issue:
9(8), P. 902 - 902
Published: Aug. 8, 2023
Micropropagation
has
an
important
role
in
the
large-scale
production
of
blackberry
plant
material,
given
high
proliferation
rates
this
species.
The
aim
present
study
was
to
evaluate
proliferative
capacity
grown
vitro
on
wheat
starch-gelled
culture
medium
compared
classical
agar-gelled
and
assess
genetic
fidelity
between
proliferated
shoots
their
mother
plants.
Six
varieties
(‘Čačanska
Bestrna’,
‘Chester
Thornless’,
‘Driscoll’s
Victoria’,
‘Loch
Ness’,
‘Polar’,
‘Karaka
Black’)
were
tested.
For
proliferation,
Murashige
Skoog
(MS)
supplemented
with
0.5
mg
dm−3
6-benzyladenine
(BA)
used.
conventional
gelled
0.5%
agar,
starch
used
as
alternative
gelling
agent
a
concentration
5%.
results
showed
that
for
all
cultivars,
highest
number
shoots/inoculum
obtained
medium,
maximum
value
54.42
±
4.18
presented
by
Black’.
Considering
length
shoots,
tested
cultivars
outstanding
5%
starch.
values
regarding
observed
Thornless’
followed
‘Čačanska
Ness’
5.55
0.04
cm,
5.46
0.06
5.37
0.09
respectively.
uniformity
micropropagated
relation
plants
confirmed
sequence-related
amplified
polymorphism
(SRAP)
start
codon
targeted
(SCoT)
molecular
markers.
Agronomy,
Journal Year:
2023,
Volume and Issue:
13(3), P. 730 - 730
Published: Feb. 28, 2023
The
instability
of
in
vitro
cultures
may
cause
genetic
and
epigenetic
changes
crops
called
somaclonal
variations.
Sometimes,
these
produce
beneficial
effects;
for
example,
they
can
be
used
breeding
programs
to
generate
new
cultivars
with
desirable
characteristics.
In
this
article,
we
present
a
systematic
review
designed
answer
the
following
question:
How
does
variation
contribute
plant
improvement?
Five
electronic
databases
were
searched
articles
based
on
pre-established
inclusion
exclusion
criteria
standardized
search
string.
technique
has
been
most
frequently
applied
ornamental
plants,
49
species
cited
48
articles,
main
agricultural
crops,
including
sugarcane,
rice,
banana,
potato
wheat,
different
countries
worldwide.
69
studies,
was
evaluate
diversity
generated
between
clones,
and,
63
agronomic
performance
characteristics
evaluated.
Other
studies
are
related
resistance
pathogens,
abiotic
stresses.
application
growth
regulators
(PGRs)
benzylaminopurine
(BAP)
dichlorophenoxyacetic
acid
(2,4-D)
common
method
generating
somaclones,
randomly
amplified
polymorphic
DNA
(RAPD)
molecular
markers
commonly
identification
characterization.
Somaclonal
improvement
economically
important
world,
supporting
launch
genotypes
resistant
diseases,
pests
However,
much
remains
explored,
such
as
mechanisms
from
which
is
derived.
Nanotechnology Science and Applications,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 35 - 56
Published: Feb. 1, 2025
Studying
the
role
of
nanoparticles
in
plant
cryopreservation
is
essential
for
developing
innovative
methods
to
conserve
genetic
resources
amid
environmental
challenges.
This
research
investigated
effects
gold
(AuNPs),
silver
(AgNPs),
and
zinc
oxide
(ZnONPs)
on
structural
integrity,
stability,
metabolic
activity
cryopreserved
materials
with
medicinal
properties.
Shoot
tips
from
two
bleeding
heart
(Lamprocapnos
spectabilis
(L).
Fukuhara)
cultivars,
'Gold
Heart'
'Valentine',
were
using
encapsulation-vitrification
technique,
added
at
concentrations
5
or
15
ppm
during
either
preculture
phase
alginate
bead
matrix
formation.
Post-recovery,
plants
underwent
histological,
molecular,
biochemical
analyses.
Electron
microscopy
observations
LN-derived
material
confirmed
production
micro-morpho-structurally
stable
cells.
It
was
found
that
could
penetrate
cell
accumulate
its
various
compartments,
including
nucleus.
As
analysis,
SCoT
markers
identified
polymorphisms
11.5%
plants,
while
RAPDs
detected
mutations
1.9%
'Valentine'
specimens.
Analysis
Molecular
Variance
(AMOVA)
indicated
cultivar,
all
variation
within
populations
not
significantly
affected
by
nanoparticle
treatments.
In
Heart',
majority
(94%)
populations,
6%
attributed
treatments
(mostly
application
ZnONPs).
The
influenced
profile
particularly
affecting
synthesis
phenolic
acids
aldehydes,
as
well
antioxidant
mechanisms
both
cultivars.
content
proteins
altered
but
'Valentine'.
results
suggest
different
types
NPs
have
varying
specific
metabolites,
which
be
harnessed
modulate
secondary
metabolism
desired
pharmacological
outcomes.
Phytomedicine,
Journal Year:
2024,
Volume and Issue:
129, P. 155667 - 155667
Published: April 22, 2024
It
has
been
a
current
research
hospots
using
fingerprinting
technology
for
quality
control
of
Chinese
herbal
medicines
(CHMs),
which
provides
scientific
basis
establishment
overall
in
accordance
with
the
characteristics
CHMs.
The
CHMs
is
diverse,
and
field
covers
many
disciplines,
such
as
analytical
chemistry,
pharmacology,
pharmaceutics,
biochemistry,
molecular
biology.
To
effectively
understand
key
areas
future
directions
regarding
fingerprint
adulteration
this
paper
analyzed
879
articles
Web
Science
Core
Collection
from
2000
to
2023
CiteSpace
VOSviewer,
systematically
assessed
process,
hotspots,
topic
distribution
among
etc.
most
prominent
contributors
are
mainly
China,
India,
United
States,
England,
Brazil.
knowledge
domains
focus
on
topics
authentication,
DNA
barcoding,
HPLC,
near-infrared
spectroscopy,
manage
data,
chemometrics,
electrochemical
fingerprinting.
Most
countries
have
recognized
pharmaceutical
potential
natural
products,
paid
more
attention
past
decade.
Future
tends
identification
chromatographic
controlling
This
valuable
reference
scholars
related
fields
analyze
existing
results,
development
trend,
explore
new
directions.
BMC Plant Biology,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: April 3, 2024
Apple
is
an
important
fruit
crop
that
always
in
demand
due
to
its
commercial
and
nutraceutical
value.
Also,
the
requirement
for
quality
planting
material
this
new
plantations
increasing
continuously.
In-vitro
propagation
alternative
approach,
which
may
help
produce
genetically
identical
high
grade
material.
In
study,
first
time,
efficient
reproducible
protocol
has
been
established
apple
root
stock
MM
104
via
axillary
bud.
Culturing
buds
on
Murashige
Skoog
rootstock
(MM
104)
resulted
better
in-vitro
propagation.
(MS)
basal
medium
supplemented
with
3.0%
(w/v)
sucrose
0.8%
agar.
The
were
MS
BA
(5.0
µM),
NAA
(1.0
µM)
further
used
establish
invitro
protocol.
Plant
Growth
Regulators
(PGRs),
combination
was
found
most
shoot
multiplication
(100%)
produced
9.8
shoots/explants
average
length
of
(2.4
±
cm).
All
shoots
roots
0.1
µM
IBA
a
5-day
dark
period.
Acclimatization
raised
plantlets
obtained
vermiculite:
perlite:
sand:
soil
(2:2:1:1)
resulting
76%
survival
under
field
conditions.
study
showed
use
bud
multiple-shoot
production
104).
This
comprehensive
report
growth
assessment
genetic
stability
using
DNA
fingerprinting
profiles
based
Inter
Simple
Sequence
Repeats
(ISSR)
Start
Codon
Targeted
(SCoT).
in-vitro-produced
plants,
as
determined
by
SCoT
ISSR
primers,
demonstrated
closeness
mother
plant.
PeerJ,
Journal Year:
2025,
Volume and Issue:
13, P. e18814 - e18814
Published: Jan. 6, 2025
Pot
marigold
is
an
ornamental
plant
of
great
importance
in
pharmacy
and
cosmetology.
However,
there
limited
published
information
on
the
diversity
species
at
both
morphological
genetic
levels.
This
paper
aimed
to
determine
selected
varieties.
The
research
focused
varieties
with
different
flower
colours,
most
important
features
flowers
height
were
analysed.
Genetic
analyses
performed
using
start
codon
target
(SCoT)
markers.
correlation
coefficients
between
analysed
studied
showed
a
significant
positive
relationship
height,
number
inflorescences
per
inflorescence
diameter.
grouped
according
colour
their
flowers.
results
obtained
our
work
indicate
that,
despite
variability
observed
features,
gene
pool
limited.
can
be
valuable
for
breeders,
particularly
selecting
suitable
crossbreeding
breeding
programs.
Additionally,
it
offers
insights
into
resources
highlights
bioconservation.
