Infrapatellar fat pad-derived MSC response to inflammation and fibrosis induces an immunomodulatory phenotype involving CD10-mediated Substance P degradation DOI Creative Commons
Dimitrios Kouroupis,

Annie C. Bowles,

Melissa A. Willman

et al.

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: July 26, 2019

The infrapatellar fat pad (IFP) serves as a reservoir of Mesenchymal Stem Cells (MSC), and with adjacent synovium plays key roles in joint disease including the production Substance P (SP) affecting local inflammatory responses transmitting nociceptive signals. Here, we interrogate human IFP-derived MSC (IFP-MSC) reaction to pro-fibrotic environments (cell priming by TNFα/IFNγ TNFα/IFNγ/CTGF exposure respectively), compared bone marrow-derived (BM-MSC). Naïve IFP-MSC exhibit increased clonogenicity chondrogenic potential BM-MSC. Primed cells experienced dramatic phenotypic changes, sharp increase CD10, upregulation immunomodulatory transcripts, secreted growth factors/cytokines pathways (IL-10, TNF-α, MAPK, Ras PI3K-Akt). Naïve, more so primed (both) induced SP degradation vitro, reproduced their supernatants abrogated thiorphan, CD10 inhibitor. These findings were vivo rat model acute synovitis, where transiently engrafted reduction. Functionally, demonstrated sustained antagonism activated peripheral blood mononuclear (PBMC) proliferation, significantly outperforming declining dose-dependent effect naïve cohorts. Collectively, our vitro data supports cell way enhance immunoregulatory properties IFP-MSC, which selectively engraft areas active synovitis/IFP fibrosis inducing degradation, resulting cell-based product alternative BM-MSC potentially treat degenerative/inflammatory diseases.

Language: Английский

Chronic Pain: Structural and Functional Changes in Brain Structures and Associated Negative Affective States DOI Open Access
Seoyon Yang, Min Cheol Chang

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(13), P. 3130 - 3130

Published: June 26, 2019

Chronic pain is a condition in which progresses from an acute to chronic state and persists beyond the healing process. impairs function decreases patients’ quality of life. In recent years, efforts have been made deepen our understanding develop better treatments alleviate pain. this review, we summarize results previous studies, focusing on mechanisms underlying development identification neural areas related We review association between negative affective states. Further, describe structural functional changes brain structures that accompany chronification discuss various neurotransmitter families involved. Our aims provide guidance for future therapeutic approaches could be used management

Language: Английский

Citations

275
Atopic dermatitis: Role of the skin barrier, environment, microbiome, and therapeutic agents DOI
Thomas A. Luger, Masayuki Amagai, Brigitte Dréno

et al.

Journal of Dermatological Science, Journal Year: 2021, Volume and Issue: 102(3), P. 142 - 157

Published: May 2, 2021

Language: Английский

Citations

151
Somatosensory and autonomic neuronal regulation of the immune response DOI

Swalpa Udit,

Kimbria J. Blake,

Isaac M. Chiu

et al.

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(3), P. 157 - 171

Published: Jan. 7, 2022

Language: Английский

Citations

134
Low back pain and osteoarthritis pain: a perspective of estrogen DOI Creative Commons
Huiwen Pang, Shihui Chen, David M. Klyne

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Aug. 4, 2023

Abstract Low back pain (LBP) is the world’s leading cause of disability and increasing in prevalence more rapidly than any other condition. Intervertebral disc (IVD) degeneration facet joint osteoarthritis (FJOA) are two common causes LBP, both occur frequently elderly women populations. Moreover, (OA) OA pain, regardless joint, experienced by up to twice as many men, this difference amplified during menopause. Changes estrogen may be an important contributor these states. Receptors for have been found within IVD tissue nearby joints, highlighting potential roles surrounding IVDs joints. In addition, supplementation has shown effective at ameliorating progression, indicating its use a therapeutic agent people with LBP pain. This review comprehensively examines relationship between conditions summarizing recent preclinical clinical findings. The molecular mechanisms which relieve associated FJOA discussed.

Language: Английский

Citations

52
Tachykinins: Neuropeptides That Are Ancient, Diverse, Widespread and Functionally Pleiotropic DOI Creative Commons
Dick R. Nässel, Meet Zandawala, Tsuyoshi Kawada

et al.

Frontiers in Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: Nov. 20, 2019

Tachykinins (TKs) are ancient neuropeptides present throughout the bilaterians and are, with some exceptions, characterized by a conserved FX1GX2Ramide carboxy terminus among protostomes FXGLMamide in deuterostomes. The best-known TK is vertebrate substance P, which mammals, together other TKs, has been implicated health disease important roles pain, inflammation, cancer, depressive disorder, immune system, gut function, hematopoiesis, sensory processing, hormone regulation. invertebrate TKs also known to have multiple functions central nervous system intestine these investigated more detail fly Drosophila arthropods. Here, we review protostome deuterostome organization evolution of precursors, peptides their receptors, as well functions, appear be partly across Bilateria. We outline distribution brains representative organisms. In Drosophila, recent studies revealed early olfactory neuromodulation circuits controlling locomotion food search, nociception, aggression, metabolic stress, release. signaling regulates lipid metabolism intestine. crustaceans, an neuromodulator rhythm-generating motor stomatogastric presynaptic modulator photoreceptor cells. Several additional functional can inferred from various brain circuits. addition, there few interesting cases where injected into prey animals vasodilators salivary glands or paralyzing agents venom glands. cases, produced predator sequences mimicking TKs. Lastly, TK-signaling appears duplicated Panarthropoda (comprising arthropods, onychophores, tardigrades) give rise novel type peptides, natalisins, distinct receptor. natalisins general, it that widely distributed act at short range neuromodulators cotransmitters.

Language: Английский

Citations

107
Current herbal medicine as an alternative treatment in dentistry: In vitro, in vivo and clinical studies DOI
Ehsan Tafazoli Moghadam, Mohsen Yazdanian, Elahe Tahmasebi

et al.

European Journal of Pharmacology, Journal Year: 2020, Volume and Issue: 889, P. 173665 - 173665

Published: Oct. 22, 2020

Language: Английский

Citations

103
Intra-CSF AAV9 and AAVrh10 Administration in Nonhuman Primates: Promising Routes and Vectors for Which Neurological Diseases? DOI Creative Commons

Karim Bey,

Johan Deniaud,

Laurence Dubreil

et al.

Molecular Therapy — Methods & Clinical Development, Journal Year: 2020, Volume and Issue: 17, P. 771 - 784

Published: April 11, 2020

The identification of the most efficient method for whole central nervous system targeting that is translatable to humans and safest route adeno-associated virus (AAV) administration a major concern future applications in clinics. Additionally, as many AAV serotypes were identified gene introduction into brain spinal cord, another key human gene-therapy success determine serotype. In this study, we compared lumbar intrathecal through catheter implantation intracerebroventricular cynomolgus macaque. We also evaluated two are currently used clinical trials: AAV9 AAVrh10. demonstrated delivery using achieved consistent transgene expression motor neurons cord neurons/glial cells several regions, whereas led brain. contrast, AAVrh10 rare neuron targeting. Finally, found efficiently targets respiratory skeletal muscles after injection cerebrospinal fluid (CSF), which represents an outstanding new property can be useful treatment diseases affecting both muscle.

Language: Английский

Citations

76
Chronic Pain in Musculoskeletal Diseases: Do You Know Your Enemy? DOI Open Access
Roberto Bonanni, Ida Cariati, Virginia Tancredi

et al.

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(9), P. 2609 - 2609

Published: May 6, 2022

Musculoskeletal pain is a condition that characterises several diseases and represents constantly growing issue with enormous socio-economic burdens, highlighting the importance of developing treatment algorithms appropriate to patient’s needs effective management strategies. Indeed, algic must be assessed treated independently underlying pathological process since it has an extremely negative impact on emotional psychic aspects individual, leading isolation depression. A full understanding pathophysiological mechanisms involved in nociceptive stimulation central sensitization important step improving approaches musculoskeletal pain. In this context, bidirectional relationship between immune cells neurons nociception could represent key point these mechanisms. Therefore, we provide updated overview magnitude problem, terms prevalence costs, summarise role most molecular players development maintenance Finally, based mechanisms, propose model, called “musculoskeletal cycle”, which useful tool counteract resignation starting for algorithm patient

Language: Английский

Citations

52
Pharmacological Methods of Pain Management: Narrative Review of Medication Used DOI Creative Commons
Nasser M. Alorfi

International Journal of General Medicine, Journal Year: 2023, Volume and Issue: Volume 16, P. 3247 - 3256

Published: July 1, 2023

Background: Pain management is a critical aspect of healthcare, aimed at alleviating discomfort and improving the quality life for individuals experiencing acute or chronic pain. Pharmacological methods constitute primary approach to pain management, including diverse array drugs that work through different mechanisms. Aim: Identifying medications commonly employed in focusing on their mechanism actions, uses, efficacy pharmacological applications. Methods: The methodology involved systematic search scientific literature using various databases, PubMed, Scopus, Google Scholar. Relevant articles published between 2000 2023 were screened inclusion. selected studies encompassed original research, review articles, therapeutic guidelines randomized controlled trials. Results: findings this suggest multimodal combining analgesics can enhance relief while minimizing adverse effects. It emphasizes importance assessing intensity, determining underlying etiology, utilizing evidence-based optimize outcomes. Conclusion: are an essential component strategies achieve optimal article concludes with discussion emerging trends future directions novel drug targets advances delivery systems. Keywords: pain, pharmacology, neuroscience, analgesia, hypersensitivity

Language: Английский

Citations

40
Activation of Mast Cells by Neuropeptides: The Role of Pro-Inflammatory and Anti-Inflammatory Cytokines DOI Open Access
D Lauritano, F Mastrangelo, Cristian D’Ovidio

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4811 - 4811

Published: March 2, 2023

Mast cells (MCs) are tissue that derived from bone marrow stem contribute to allergic reactions, inflammatory diseases, innate and adaptive immunity, autoimmunity, mental disorders. MCs located near the meninges communicate with microglia through production of mediators such as histamine tryptase, but also secretion IL-1, IL-6 TNF, which can create pathological effects in brain. Preformed chemical inflammation tumor necrosis factor (TNF) rapidly released granules MCs, only immune capable storing cytokine although it be produced later mRNA. The role nervous system diseases has been extensively studied reported scientific literature; is great clinical interest. However, many published articles concern studies on animals (mainly rats or mice) not humans. known interact neuropeptides mediate endothelial cell activation, resulting central (CNS) In brain, neurons causing neuronal excitation release cytokines chemokines. This article explores current understanding MC activation by neuropeptide substance P (SP), corticotropin-releasing hormone (CRH), neurotensin, pro-inflammatory cytokines, suggesting a therapeutic effect anti-inflammatory IL-37 IL-38.

Language: Английский

Citations

31