Potential Neuroprotective Effect of Melatonin in the Hippocampus of Male BTBR Mice DOI Open Access
Matteo Bonetti, Lorena Giugno, Elisa Borsani

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(11), P. 1652 - 1652

Published: May 28, 2024

Autism spectrum disorder (ASD) is a neurodevelopmental identified by impairments in common social interactions and repetitive behaviors. In ASD patients, substantial morphological alterations have been observed the hippocampus, which represents an important region for development of skills. Melatonin, commonly found many foods plants, also produced pineal gland. This indolamine, known to regulate circadian rhythm, shows antioxidant anti-inflammatory properties. We therefore hypothesized that melatonin may reduce oxidative stress inflammation hippocampus patients. explored our hypothesis using BTBR mouse, well-regarded murine transgenic model ASD. Immediately after weaning, male C57BL/6 mice underwent 8-week treatment with or vehicle. Later, through immunohistochemistry immunoblotting analysis we evaluated overall expression cellular localization Nrf2 SOD1, two enzymes involved response. Similarly, NLRP3 NFkB, mediators inflammation, GAD67, enzyme responsible synthesis GABA. Ultimately, addressed melatonin’s potential iron metabolism DAB-enhanced Perls reaction assay. Results showed modulating analyzed markers mice, suggesting neuroprotective effect

Language: Английский

TRPA1-PI3K/Akt-OPA1-ferroptosis axis in ozone-induced bronchial epithelial cell and lung injury DOI Creative Commons

Jiali Weng,

Qi Liu, Chenfei Li

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170668 - 170668

Published: Feb. 5, 2024

Transient receptor potential (TRP) ankyrin 1 (TRPA1) could mediate ozone-induced lung injury. Optic Atrophy (OPA1) is one of the significant mitochondrial fusion proteins. Impaired fusion, resulting in dysfunction and ferroptosis, may drive onset progression In this study, we examined whether TRPA1 mediated bronchial epithelial cell injury by activating PI3K/Akt with involvement OPA1, leading to ferroptosis. Wild-type, TRPA1-knockout (KO) mice (C57BL/6 J background) ferrostatin-1 (Fer-1)-pretreated were exposed 2.5 ppm ozone for 3 h. Human (BEAS-2B) cells treated h presence inhibitor A967079 or TRPA1-knockdown (KD) as well pharmacological modulators PI3K/Akt-OPA1-ferroptosis. Transcriptome was used screen decipher differential gene expressions pathways. Oxidative stress, inflammation ferroptosis measured together morphology, function dynamics. Acute exposure induced airway hyperresponsiveness (AHR), reduced enhanced mice. Similarly, acute inflammatory responses, altered redox abnormal structure function, BEAS-2B cells. There increased decreased responses ozone-exposed TRPA1-KO Fer-1-pretreated TRPA1-KD OPA1 prevented through pathway These vitro results further confirmed modulator experiments. Exposure induces human mouse lungs TRPA1, which via a PI3K/Akt/OPA1 axis. This supports role blockade preventing deleterious effects ozone.

Language: Английский

Citations

12
Ginkgolide B binds to GPX4 and FSP1 to alleviate cerebral ischemia/reperfusion injury in rats DOI

Rong Zou,

Zhaoxia Liu, Peng Wang

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117237 - 117237

Published: Jan. 1, 2025

Language: Английский

Citations

1
Synergistic anti-oxidative/anti-inflammatory treatment for acute lung injury with selenium based chlorogenic acid nanoparticles through modulating Mapk8ip1/MAPK and Itga2b/PI3k-AKT axis DOI Creative Commons

Huizhen Xing,

Xueli Bai,

Xiaoying Pei

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
Potential Role of Natural Antioxidants in Countering Reperfusion Injury in Acute Myocardial Infarction and Ischemic Stroke DOI Creative Commons
Sofía Orellana-Urzúa, Camilo Briones-Valdivieso, Silvia Chichiarelli

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1760 - 1760

Published: Sept. 13, 2023

Stroke and acute myocardial infarction are leading causes of mortality worldwide. The latter accounts for approximately 9 million deaths annually. In turn, ischemic stroke is a significant contributor to adult physical disability globally. While reperfusion crucial tissue recovery, it can paradoxically exacerbate damage through oxidative stress (OS), inflammation, cell death. Therefore, imperative explore diverse approaches aimed at minimizing ischemia/reperfusion injury enhance clinical outcomes. OS primarily arises from an excessive generation reactive oxygen species (ROS) and/or decreased endogenous antioxidant potential. Natural compounds counteract the mechanisms linked ROS. promising preclinical results, based on monotherapies, account protective effects against by ROS, translating these models into human applications has yielded controversial evidence. However, since wide spectrum antioxidants having chemical characteristics offers varied biological actions signaling pathways, multitherapy emerged as valuable therapeutic resource. Moreover, combination in holds potential synergistic effects. This study was designed with aim providing updated overview natural suitable preventing cerebral injuries.

Language: Английский

Citations

22
Neuroprotective effect of Dl-3-n-butylphthalide against ischemia–reperfusion injury is mediated by ferroptosis regulation via the SLC7A11/GSH/GPX4 pathway and the attenuation of blood–brain barrier disruption DOI Creative Commons

Shuangli Xu,

Xuewei Li, Yutian Li

et al.

Frontiers in Aging Neuroscience, Journal Year: 2023, Volume and Issue: 15

Published: Feb. 23, 2023

Stroke is one of the most severe diseases worldwide, resulting in physical and mental problems. Dl-3-n-butylphthalide, a compound derived from celery seed, has been approved for treating ischemic stroke China. No study evaluated how Dl-3-n-butylphthalide affects ferroptosis SLC7A11/GSH/GPX4 signal pathway blood-brain barrier (BBB) PDGFRβ/PI3K/Akt pathways rat middle cerebral artery occlusion/reperfusion (MCAO/R) model stroke.Sprague-Dawley rats were used to develop MCAO/R model. Our three incremental doses (10, 20, 30) injected intraperitoneally 24 h after surgery. The neuroprotective effect success using neurofunction score, brain water content determination, triphenyl-tetrazolium chloride-determined infarction area changes. Pathological changes tissue degree apoptosis examined by hematoxylin eosin, Nissl, terminal deoxynucleotidyl transferase dUTP nick end labeling staining. In addition, proteins RNA expression levels studied verify effects Dl-3-n-butyphthalide on both pathways. At same time, commercial kits detect glutathione, reactive oxygen species, malondialdehyde, oxidative stress tissues.The dose not only improved MCAO-induced dysfunction alleviated pathological damage, inflammatory response, stress, but also protected against reduced BBB damage. These resulted neurological function cortex.We speculate that focal ischemia/reperfusion, which may be mediated through ferroptosis-dependent PDGFRβ/PI3/Akt pathway.

Language: Английский

Citations

21
Ferroptosis: a potential therapeutic target for stroke DOI Creative Commons
Chengli Liu, Guijun Wang, Wenrui Han

et al.

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(5), P. 988 - 997

Published: Sept. 22, 2023

Ferroptosis is a form of regulated cell death characterized by massive iron accumulation and iron-dependent lipid peroxidation, differing from apoptosis, necroptosis, autophagy in several aspects. regarded as critical mechanism series pathophysiological reactions after stroke because overload caused hemoglobin degradation metabolism imbalance. In this review, we discuss ferroptosis-related metabolisms, important molecules directly or indirectly targeting transcriptional regulation ferroptosis, revealing the role ferroptosis progression stroke. We present updated progress intervention therapeutic strategies for vivo vitro summarize effects inhibitors on Our review facilitates further understanding pathogenesis stroke, proposes new targets treatment suggests that more efforts should be made to investigate

Language: Английский

Citations

19
A single mitochondria-targetable fluorescent probe for visualizing cysteine and glutathione in ferroptosis of myocardial ischemia/reperfusion injury DOI
Jiayu Zeng,

Minhui Liu,

Ting Yang

et al.

Talanta, Journal Year: 2023, Volume and Issue: 270, P. 125610 - 125610

Published: Dec. 28, 2023

Language: Английский

Citations

18
Crosstalk between ferroptosis and necroptosis in cerebral ischemia/reperfusion injury and Naotaifang formula exerts neuroprotective effect via HSP90-GCN2-ATF4 pathway DOI
Yue Zhou, Ruining She, Zhigang Mei

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155399 - 155399

Published: Feb. 1, 2024

Language: Английский

Citations

7
Genetically Engineered Membrane‐Coated Nanoparticles for Enhanced Prostate‐Specific Membrane Antigen Targeting and Ferroptosis Treatment of Castration‐Resistant Prostate Cancer DOI Creative Commons
Yu Li, Hongji Li, Keying Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(33)

Published: July 1, 2024

Conventional androgen deprivation therapy (ADT) targets the receptor (AR) inhibiting prostate cancer (PCa) progression; however, it can eventually lead to recurrence as castration-resistant PCa (CRPC), which has high mortality rates and lacks effective treatment modalities. The study confirms presence of glutathione peroxidase 4 (GPX4) expression, a key regulator ferroptosis (i.e., iron-dependent program cell death) in CRPC cells. Therefore, inducing cells might be an therapeutic modality for CRPC. However, nonspecific uptake inducers result undesirable cytotoxicity major organs. Thus, precisely induce cells, genetic engineering strategy is proposed embed prostate-specific membrane antigen (PSMA)-targeting antibody fragment (gy1) macrophage membrane, then coated onto mesoporous polydopamine (MPDA) nanoparticles produce biomimetic nanoplatform. results indicate that membrane-coated (MNPs) exhibit specificity affinity toward On further encapsulation with RSL3 iron ions, MPDA/Fe/RSL3@M-gy1 demonstrates superior synergistic effects highly targeted eliciting significant efficacy against tumor growth bone metastasis without increased cytotoxicity. In conclusion, new reported PSMA-specific, CRPC-targeting platform induction safety.

Language: Английский

Citations

6
Downregulation of Nogo-B ameliorates cerebral ischemia/reperfusion injury in mice through regulating microglia polarization via TLR4/NF-kappaB pathway DOI
Peng Gong, Hui-Yu Jia, Rui Li

et al.

Neurochemistry International, Journal Year: 2023, Volume and Issue: 167, P. 105553 - 105553

Published: May 23, 2023

Language: Английский

Citations

15