Biodiversitas Journal of Biological Diversity,
Journal Year:
2023,
Volume and Issue:
24(8)
Published: Aug. 25, 2023
Abstract.
Oktiarni
D,
Hermansyah,
Ibrahim
E,
Marsi,
Hasanudin,
Miksusanti,
Yanto
DHY,
Rahmani
N,
Kasmiarti
G.
2023.
Short
Communication:
The
isolation
of
Klebsiella
variicola’s
cellulase
from
Macrotermes
gilvus
gut
in
Indralaya
Peatlands,
Indonesia.
Biodiversitas
24:
4218-4222.
Termites
are
organisms
that
can
inflict
damage
on
agricultural,
forest,
and
furniture
materials.
Furthermore,
they
positively
affect
the
ecosystem
due
to
their
ability
break
down
lignocellulose-containing
According
previous
findings,
cellulolytic
bacteria
termite
obtained
Peatlands
showed
activity
hydrolyzed
cellulose
a
CMC
agar
medium.
In
this
study,
enzymes
variicola
isolated
higher
indexes
after
staining
with
Congo
red.
enzyme
was
purified
characterized
using
ammonium
sulfate
precipitation
dialysis.
SDS
PAGE
zymogram
analysis
reported
molecular
mass
20
kDa,
optimal
at
pH
6
temperature
50°C.
Engineering Microbiology,
Journal Year:
2024,
Volume and Issue:
4(2), P. 100139 - 100139
Published: Feb. 2, 2024
Lignocellulosic
biomass
is
an
abundant
and
renewable
bioresource
for
the
production
of
biofuels
biochemical
products.
The
classical
biorefinery
process
lignocellulosic
degradation
conversion
comprises
three
stages,
i.e.,
pretreatment,
enzymatic
saccharification,
fermentation.
However,
complicated
pretreatment
process,
high
cost
cellulase
production,
insufficient
performance
fermentation
strains
have
restricted
industrialization
biorefinery.
Consolidated
bioprocessing
(CBP)
technology
combines
enzyme
in
a
single
bioreactor
using
specific
microorganism
or
consortium
microbes
represents
another
approach
worth
exploring
chemicals
from
biomass.
present
review
summarizes
progress
made
research
CBP
conversion.
In
this
review,
different
strategies
lignocellulose
are
reviewed,
including
with
natural
lignocellulose-degrading
microorganisms
as
chassis,
biosynthetic
microbial
co-culturing
systems.
This
provides
new
perspectives
insights
on
utilization
low-cost
feedstock
biochemicals.
Molecules,
Journal Year:
2021,
Volume and Issue:
26(17), P. 5411 - 5411
Published: Sept. 6, 2021
Lignocellulose
is
a
kind
of
renewable
bioresource
containing
abundant
polysaccharides,
which
can
be
used
for
biochemicals
and
biofuels
production.
However,
the
complex
structure
hinders
final
efficiency
lignocellulosic
biorefinery.
This
review
comprehensively
summarizes
hydrolases
typical
microorganisms
degradation.
Moreover,
commonly
bioprocesses
biorefinery
are
also
discussed,
including
separated
hydrolysis
fermentation,
simultaneous
saccharification
fermentation
consolidated
bioprocessing.
Among
these
methods,
construction
microbial
co-culturing
systems
via
bioprocessing
regarded
as
potential
strategy
to
efficiently
produce
biofuels,
providing
theoretical
direction
constructing
efficient
stable
process
system
in
future.
E3S Web of Conferences,
Journal Year:
2025,
Volume and Issue:
606, P. 05007 - 05007
Published: Jan. 1, 2025
Biomass
refers
to
the
biodegradable
portion
of
products
and
residues
from
agriculture,
forestry,
related
industries,
including
municipal
industrial
waste.
It
encompasses
molecular
macromolecular
compounds
derived
plants
other
organic
sources.
energy,
considered
sustainable
eco-friendly,
can
reduce
greenhouse
gas
emissions
enhance
energy
security,
contributing
significantly
net-zero
emission
goals.
Various
methods
for
biomass
utilization
include
thermochemical
biochemical
conversions,
as
well
co-production.
Thermochemical
conversion
like
pyrolysis,
gasification,
torrefaction
transform
into
fuels
chemicals,
offering
solutions
waste
management
renewable
energy.
Biochemical
methods,
such
anaerobic
digestion
fermentation,
use
biological
processes
produce
biogas
biofuels.
Co-production,
combining
with
sources
(e.g.,
geothermal
or
coal),
optimizes
resource
increases
efficiency.
While
challenges
remain,
cost
technical
barriers,
provides
an
effective,
carbon-neutral
path
reducing
fossil
fuel
dependence.
The
paper
highlights
energy’s
diverse
technologies
its
importance
in
development
future
landscape.
Environmental Technology & Innovation,
Journal Year:
2022,
Volume and Issue:
27, P. 102805 - 102805
Published: July 4, 2022
The
study
aims
to
optimize
cellulase
(CMCase)
production
by
Aspergillus
flavus
using
wheat
straw,
an
abundantly
available
lignocellulosic
waste,
as
a
substrate.
Three
parameters,
i.e.,
nitrogen
content
(0.25
1%),
fungal
inoculum
and
duration
(3
12
days),
were
optimized
for
maximum
CMCase
Response
surface
methodology-Box
Behnken
design
(RSM-BBD).
quadratic
response
was
suitable,
the
model
significant.
However,
higher-order
machine
learning
(ML)
models
applied
RSM-BBD
had
low
R2
value
(0.85)
negative
predicted
(−0.82).
supervised
ML
regression
models,
Artificial
neural
network
(ANN)
with
Bayesian
Regularization
Neural
Network
(BRNN)
Radial
Basis
function
(RBFNN),
Support
vector
(SVM)
Polynomial
kernel
(SPK),
Gaussian
(SGK),
process
learner
(GPL)
exponential
(GEK)
squared
(GSEK)
applied.
RBFNN
best
performing
mean
error
(MSE)
of
0.0025
0.98.
13.89
U/gds
at
yeast
extract
0.25%,
0.625%,
days.
There
almost
threefold
increase
in
after
optimization
compared
screening
experiments
(4.7
U/gds).
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: Aug. 19, 2023
Lignocellulosic
biomass
is
the
most
abundant
and
renewable
terrestrial
raw
material
for
conversion
into
bioproducts
biofuels.
However,
low
utilization
efficiency
of
lignocellulose
causes
environmental
pollution
resource
waste,
which
limits
large-scale
application
bioconversion.
The
degradation
by
microorganisms
an
efficient
cost-effective
way
to
overcome
challenge
utilizing
plant
resources.
This
work
aimed
screen
valuable
cellulolytic
bacteria,
explore
its
molecular
mechanism
from
genomic
insights,
investigate
ability
strain
biodegrade
wheat
straw.
Asia-Pacific Journal of Molecular Biology and Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 41 - 53
Published: April 10, 2025
The
symbiotic
relationship
between
termites
and
their
gut
microbes
is
one
of
nature's
most
fascinating
mutualistic
interactions.
Termites,
renowned
for
ability
to
decompose
lignocellulosic
materials
such
as
wood,
rely
heavily
on
microbiota,
which
includes
bacteria,
archaea,
protozoa,
each
playing
specific
roles
in
breaking
down
cellulose,
hemicellulose,
lignin.
This
complex
partnership
not
only
enables
thrive
low-nutrient
environments
but
also
supports
nitrogen
metabolism
provides
essential
nutrients
survival.
termite
highly
compartmentalized,
creating
various
microenvironments
that
support
diverse
microbial
communities
facilitate
metabolic
processes,
including
fermentation
anoxic
conditions.
Shaped
by
evolutionary
pressures,
this
has
resulted
specialized
adaptations
optimize
lignocellulose
degradation
cycling.
review
explores
the
mechanisms
termite-microbe
symbiosis,
functional
across
different
taxa,
structural
compartmentalization
gut,
dynamics
driving
relationship.
In
addition,
highlights
ecological
biotechnological
significance
offering
valuable
insights
into
efficient
nutrient
recycling
could
inspire
future
applications
sustainable
bioenergy
waste
management.
