Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: June 5, 2025
Cyanine
dyes
usually
serve
as
good
fluorescence
probes
but
not
efficient
photosensitizers
owing
to
the
spin-forbidden
intersystem
crossing
process
and
short
excited-state
lifetime.
Nevertheless,
structure-derived
energy
release
from
excited
state
could
enable
function
efficiently.
In
this
work,
we
developed
cyanine
dimers
(2o-Cy,
2m-Cy,
2p-Cy)
based
on
exciton
coupling
effects
by
connecting
two
Cy5.5
molecules
at
N-indole
site
with
nonconjugated
ortho/meta/para-bis(bromomethyl)benzene
linkers.
These
exhibited
significantly
enhanced
molar
extinction
coefficients
(32-45
×
104
M
cm-1)
well
satisfactory
triplet
quantum
yields
(32-44%)
lifetimes
(10.6-11.9
μs),
leading
a
substantially
reactive
oxygen
species
production
along
antitumor
activity
under
both
normoxic
hypoxic
conditions.
Furthermore,
2p-Cy
NPs
ablated
primary
tumors,
inhibited
distant
tumor
growth,
prevented
metastatic
regrowth
via
photoinduced
innate
immune
activation.
This
dimer-based
strategy
represents
powerful
approach
develop
high-performance
photosensitive
for
photodynamic
immunotherapy.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
The
two
contradictory
entities
in
nature
often
follow
the
principle
of
unity
opposites,
leading
to
optimal
overall
performance.
Particularly,
aggregation-induced
emission
luminogens
(AIEgens)
with
donor–acceptor
(D–A)
structures
exhibit
tunable
optical
properties
and
versatile
functionalities,
offering
significant
potential
revolutionize
cancer
treatment.
However,
trapped
by
low
molar
absorptivity
(ε)
owing
distorted
configurations,
ceilings
their
photon-harvesting
capability
corresponding
phototheranostic
performance
still
fall
short.
Therefore,
a
research
paradigm
from
twisted
configuration
near-planar
structure
featuring
high
ε
is
urgently
needed
for
AIEgens
development.
Herein,
introducing
strategy
"motion
stillness"
into
highly
planar
A–D–A
skeleton,
we
successfully
developed
near-infrared
(NIR)-II
AIEgen
Y5-2BO-2BTF,
which
boasts
an
impressive
1.06
×
105
M–1
cm–1
photothermal
conversion
efficiency
(PCE)
77.8%.
modification
steric
hindrance
on
benzene
ring
acceptor
unit
aggregation-caused
quenching
counterpart
Y5-2BO,
meta-CF3-substituted
naphthyl,
leads
reversely
staggered
packing
various
intermolecular
noncovalent
conformational
locks
Y5-2BO-2BTF
("stillness").
Furthermore,
−CF3
moiety
acted
as
flexible
motion
ultralow
energy
barrier,
significantly
facilitating
process
loose
aggregates
("motion").
Accordingly,
nanoparticles
enabled
tumor
eradication
pulmonary
metastasis
inhibition
through
NIR-II
fluorescence-photoacoustic-photothermal
imaging-navigated
type
I
photodynamic-photothermal
therapy.
This
work
provides
first
evidence
that
conformation
stacking
arrangement
could
serve
novel
molecular
design
direction
AIEgens,
shedding
new
light
constructing
superior
agents
bioimaging
Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(4), P. 491 - 491
Published: March 28, 2025
Background:
Endoplasmic
reticulum
(ER)-targeted
phototherapy
has
emerged
as
a
promising
approach
to
amplify
ER
stress,
induce
immunogenic
cell
death
(ICD),
and
enhance
anti-tumor
immunity.
However,
its
impact
on
the
antigenicity
of
dying
tumor
cells
remains
poorly
understood.
Methods:
Laser
activation
ER-targeted
photosensitizer
ER-Cy-poNO2
was
performed
investigate
effects
antigenicity.
Transcriptomic
analysis
carried
out
assess
gene
expression
changes.
Immunopeptidomics
profiling
used
identify
high-affinity
major
histocompatibility
complex
class
I
(MHC-I)
ligands.
In
vitro
functional
studies
were
conducted
evaluate
dendritic
maturation
T
lymphocyte
activation,
while
in
vivo
experiments
by
combining
identified
peptide
with
poly
IC
Results:
significantly
remodeled
antigenic
landscape
4T-1
cells,
enhancing
their
immunogenicity.
revealed
upregulation
antigen
processing
presentation
pathways.
multiple
MHC-I
ligands,
IF4G3986–994
(QGPKTIEQI)
showing
exceptional
vitro,
promoted
enhanced
lymphocytes
activation.
vivo,
combination
elicited
robust
immunity,
characterized
increased
CD8+
infiltration,
reduced
regulatory
(Tregs)
microenvironment,
elevated
systemic
Interferon-gamma
(IFN-γ)
levels,
significant
growth
inhibition
without
toxicity.
Conclusions:
These
findings
establish
mechanistic
link
between
stress-driven
ICD,
immunopeptidome
remodeling,
adaptive
immune
highlighting
potential
platform
for
identifying
peptides
advancing
peptide-based
cancer
vaccines.
ChemPhotoChem,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 10, 2025
Phototherapy,
encompassing
photodynamic
therapy
(PDT)
and
photothermal
(PTT),
presents
a
promising
cancer
treatment
strategy
with
high
spatial
specificity
minimal
invasiveness.
Optimizing
these
techniques
hinges
on
the
design
of
high‐performance
photosensitizers
(PSs)
agents
(PTAs).
Single‐atom
chalcogen
substitution,
at
both
endocyclic
exocyclic
positions
in
conventional
organic
chromophores,
has
proven
to
be
novel
for
enhancing
their
photophysical
photochemical
properties.
Ideal
PSs/PTAs
exhibit
biocompatibility,
visible‐to‐near‐infrared
(vis–NIR)
absorption,
efficient
triplet
state
harvesting,
feasible
heat
energy
transfer
efficiency.
Moreover,
chalcogen‐based
show
promise
synergistic
multimodal
therapies,
integrating
imaging
treatment.
This
review
comprehensively
examines
recent
advancements
(within
last
five
years)
utilizing
substitution
robust
PDT/PTT.
It
provides
holistic
perspective
spectroscopic
characterization
computational
analyses,
singlet‐oxygen/triplet
quantum
yields,
generation
efficiency,
vitro/in
vivo
biological
evaluations
chalcogen‐substituted
PSs/PTAs.
is
anticipated
that
this
will
stimulate
further
research
endeavors
area.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 28, 2025
Abstract
The
development
of
phototherapeutics
with
high
photothermal
conversion
efficiency
(PCE)
and
strong
ability
to
generate
reactive
oxygen
species
under
single
near-infrared
(NIR)
laser
irradiation
for
immuno-phototherapy
applications
remains
a
significant
challenge.
Herein,
we
optimally
selected
the
molecule
IT-4F
an
acceptor-donor-acceptor
(A-D-A)
structure
prepare
water-dispersible
nanoparticles
(NPs)
by
assembly
DSPE-PEG-NH2.
Such
NPs
have
NIR
absorption
fluorescence
peaks
at
728
817
nm,
respectively.
They
can
singlet
(1O2)
superoxide
anion
(O2-·)
irradiation,
1O2
generation
quantum
yield
31.5%.
also
effectively
convert
photon-energy
into
heat
PCE
42.8%.
outstanding
properties
enable
them
be
used
in
imaging
guided
therapy
(PTT),
photodynamic
(PDT).
Moreover,
PDT
PTT
triggered
immunogenic
cell
death
PANoptosis
tumor
cells,
which
not
only
inhibited
growth
metastasis
mice
model,
but
induced
robust
immune
response,
evidenced
increased
infiltration
CD8+
T
CD4+
dendritic
decreased
presence
immunosuppressive
cells
such
as
myeloid-derived
suppressor
regulatory
cells.
efficacy
organoid
human
breast
cancer
was
verified.
