Trends in Plant Science, Journal Year: 2025, Volume and Issue: 30(3), P. 232 - 234
Published: Jan. 2, 2025
Language: Английский
Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(9), P. 603 - 622
Published: April 24, 2024
Language: Английский
Citations
66
Genome biology, Journal Year: 2023, Volume and Issue: 24(1)
Published: June 29, 2023
Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor hexaploid wheat, ePPEplus, introducing a V223A substitution into reverse transcriptase the ePPEmax* architecture. ePPEplus enhances average 33.0-fold and 6.4-fold compared to original PPE ePPE, respectively. Importantly, robust multiplex platform established for simultaneous of four ten genes protoplasts up eight regenerated wheat plants at frequencies 74.5%, thus expanding applicability editors stacking multiple agronomic traits.
Language: Английский
Citations
52
Cell, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
5
Nature Plants, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Language: Английский
Citations
4
Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Language: Английский
Citations
4
Genome biology, Journal Year: 2024, Volume and Issue: 25(1)
Published: May 21, 2024
Abstract Background High-efficiency prime editing (PE) is desirable for precise genome manipulation. The activity of mammalian PE systems can be largely improved by inhibiting DNA mismatch repair coexpressing a dominant-negative variant MLH1. However, this strategy has not been widely used optimization in plants, possibly because its less conspicuous effects and inconsistent performance at different sites. Results We show that direct RNAi knockdown OsMLH1 an ePE5c system increases the efficiency our most recently updated tool 1.30- to 2.11-fold stably transformed rice cells, resulting as many 85.42% homozygous mutants T 0 generation. high specificity revealed whole-genome sequencing. To overcome partial sterility induced ePE5c, conditional excision introduced remove module Cre-mediated site-specific recombination. Using simple approach enriching events, we generate 100% module-free plants increase due maintained excised whose fertility impaired. Conclusions This study provides safe reliable plant improving without disturbing development via transient MMR inhibition with excisable
Language: Английский
Citations
13
Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 77 - 77
Published: Jan. 18, 2025
This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.
Language: Английский
Citations
2
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 16, 2025
High crop yields require adequate nutrients, particularly nitrate (N) and phosphorus (P). Identifying regulators for efficient N-P utilization is critical in wheat. To explore interactions, we analyze root transcriptomes under varying supplies identify TaTCP6 as a potential regulator. Nitrate-stimulated directly triggers the expression of genes related to nitrogen utilization. competes with TaSPX1/4 release TaPHR2, also interacts TaPHR2 enhance transactivation capacity downstream genes. Thus, through dual roles TaTCP6, TCP6-SPX-PHR2 module activates starvation response (PSR) Inhibiting reduces N P absorption, negatively impacting yield, while overexpressing increases grain yield. Notably, overexpression TaSPX1 suppresses genes, especially low conditions. In conclusion, our findings highlight role coordinating propose strategy reduce fertilizer inputs sustainable agriculture. A nitrate-induced transcription factor identified central regulator balanced phosphorus, moderate increase its promotes wheat
Language: Английский
Citations
1
Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7
Published: March 10, 2025
Climate change threatens global crop yield and food security due to rising temperatures, erratic rainfall, increased abiotic stresses like drought, heat, salinity. Gene editing technologies, including CRISPR/Cas9, base editors, prime offer precise tools for enhancing resilience. This review explores the mechanisms of these technologies their applications in developing climate-resilient crops address future challenges. While CRISPR/enables targeted modifications plant DNA, editors allow direct conversion without inducing double-stranded breaks, enable insertions, deletions, substitutions. By understanding manipulating key regulator genes involved stress responses, such as DREB, HSP, SOS, ERECTA, HsfA1, NHX; tolerance can be enhanced against salt stress. improve traits related root development, water use efficiency, response pathways, heat shock response, photosynthesis, membrane stability, ion homeostasis, osmotic adjustment, oxidative response. Advancements gene integration with genomics, phenomics, artificial intelligence (AI)/machine learning (ML) hold great promise. However, challenges off-target effects, delivery methods, regulatory barriers must addressed. highlights potential develop crops, contributing sustainable agriculture.
Language: Английский
Citations
1
The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)
Published: March 31, 2025
SUMMARY We identified a mutant of hexaploid wheat ( Triticum aestivum ) with impaired responses to gravity. The mutant, named Twisted Sister1 TS1 ), had agravitropic roots that were often twisted along altered shoot phenotypes. Roots insensitive externally applied auxin, the genetics and physiology suggestive mutated AUX/IAA transcription factor gene. Hexaploid possesses over 80 genes, sequence information did not identify an obvious candidate. Bulked segregant analysis F 2 population mapped mutation chromosome 5A, subsequent mapping located 41 Mbp region. RNA‐seq TraesCS5A03G0149800 gene encoding TaAUX/IAA protein be in highly conserved domain II motif. confirmed as underlying phenotype by generating transgenic Arabidopsis thaliana . Analysis data suggested broad similarities between for role genes gravity responses, although there marked differences. Here we show sequenced genome, previous knowledge from other plant species, mapping, RNA‐seq, expression have enabled cloning key defines architecture.
Language: Английский
Citations
1