Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Oct. 4, 2022
Abstract
Lead
(Pb)
is
a
corrosion-resistant,
heavy,
non-ferrous
metal
with
widespread
environmental
pollution.
Several
chelators
have
been
used
in
the
treatment
of
Pb
poisoning.
However,
effect
sodium
para-aminosalicylic
acid(PAS-Na)on
excretion
has
yet
to
be
reported.
To
investigate
effects
PAS-Na
on
excretion,
mouse
model
acute
lead
exposure
was
established.
Healthy
mice
(90)
received
abdominal
injection
(i.p.)
120
mg/kg
acetate,
and
4
h
later
80,
160,
240
PAS-Na,
or
edetate
calcium
disodium
(CaNa
2
EDTA)
were
injected
subcutaneously
(s.c.)
once
per
day
for
6
days
into
corresponding
groups,
respectively.
The
Control,
Pb-exposed,
CaNa
EDTA
groups
evaluated
simultaneously.
After
collecting
24
urine
samples,
animals
sacrificed
batches
2nd,
4th,
6th
day.
Levels
other
elements
[including
manganese
(Mn)
copper
(Cu)]
urine,
whole
blood
brain
tissues
analyzed
by
graphite
furnace
atomic
absorption
spectrometry
(AAS).
results
showed
that
increased
levels
mice,
afforded
antagonistic
against
Pb-induced
toxicity.
While
additional
studies
will
needed,
these
novel
establish
as
potential
efficacious
mitigating
Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
256, P. 114861 - 114861
Published: April 5, 2023
The
brain
barrier
is
an
important
structure
for
metal
ion
homeostasis.
According
to
studies,
lead
(Pb)
exposure
disrupts
the
transportation
of
copper
(Cu)
through
barrier,
which
may
cause
impairment
nervous
system;
however,
specific
mechanism
unknown.
previous
studies
suggested
X-linked
inhibitor
apoptosis
(XIAP)
a
sensor
cellular
Cu
level
mediate
degradation
MURR1
domain-containing
1
(COMMD1)
protein.
XIAP/COMMD1
axis
was
thought
be
regulator
in
metabolism
maintenance.
In
this
study,
role
XIAP-regulated
COMMD1
protein
Pb-induced
disorders
cells
investigated.
Pb
significantly
increased
levels
both
cell
types,
according
atomic
absorption
technology
testing.
Western
blotting
and
reverse
transcription
PCR
(RT-PCR)
showed
that
were
increased,
whereas
XIAP,
ATP7A,
ATP7B
decreased.
However,
there
no
significant
effects
at
messenger
RNA
(mRNA)
(XIAP,
ATP7B).
accumulation
expression
reduced
when
knocked
down
by
transient
small
interfering
(siRNA)
transfection.
addition,
plasmid
transfection
XIAP
before
accumulation,
levels,
decreased
levels.
conclusion,
can
reduce
expression,
increase
specifically
decrease
resulting
cells.
Metallomics,
Journal Year:
2022,
Volume and Issue:
14(12)
Published: Jan. 1, 2022
Metal
dyshomeostasis
is
a
well-established
consequence
of
neurodegenerative
diseases
and
traumatic
brain
injury.
While
the
significance
metals
continues
to
be
uncovered
in
many
neurological
disorders,
their
implication
repetitive
mild
injury
remains
uncharted.
To
address
this
gap,
we
characterized
spatial
distribution
metal
levels
(iron,
zinc,
copper)
using
laser
ablation-inductively
coupled
plasma-mass
spectrometry,
profile
metal-binding
proteins
via
size
exclusion
chromatography-inductively
spectrometry
expression
major
iron
storing
protein
ferritin
western
blotting.
Using
mouse
model
injury,
3-month-old
male
female
C57Bl6
mice
received
one
or
five
impacts
(48
h
apart).
At
1
month
following
5×
TBI
(traumatic
injury),
were
significantly
elevated
contralateral
cortex.
There
was
trend
toward
increased
entire
hemisphere
reduction
cortical
iron-binding
1×
TBI.
No
changes
zinc
seen
both
hemispheres
TBI,
although
there
ipsilateral
zinc-binding
increase
Copper
without
copper-binding
proteins.
This
study
shows
for
first
time
that
(r-mTBI)
leads
dyshomeostasis,
highlighting
its
potential
involvement
promoting
neurodegeneration,
which
provides
rationale
examining
benefit
metal-targeting
drugs,
have
shown
promising
results
conditions
single
but
yet
tested
r-mTBI.
Toxicology and Industrial Health,
Journal Year:
2023,
Volume and Issue:
39(8), P. 464 - 470
Published: July 5, 2023
Lead
(Pb)
is
a
corrosion-resistant,
heavy,
non-ferrous
metal.
Several
metal
chelators
have
been
used
for
the
treatment
of
Pb
poisoning.
However,
efficacy
sodium
para-aminosalicylic
acid
(PAS-Na)
in
enhancing
excretion
has
yet
to
be
fully
characterized.
Healthy
male
mice
(90)
were
divided
into
six
groups,
normal
control
group
was
intraperitoneally
(i.p.)
injected
with
saline
and
remaining
i.p.
120
mg/kg
acetate.
Four
hour
later,
subcutaneously
(back)
(s.c.)
(80,
160,
240
mg/kg)
PAS-Na
or
edetate
calcium
disodium
(CaNa2EDTA)
an
equivalent
amount
saline,
once
per
day
6
days.
After
24-h
urine
sample
collections,
animals
anesthetized
5%
chloral
hydrate
sacrificed
batches
on
2nd,
4th,
6th
day.
Levels
[including
manganese
(Mn)
copper
(Cu)]
urine,
whole
blood,
brain
tissues
analyzed
by
graphite
furnace
atomic
absorption
spectrometry.
The
results
showed
that
exposure
increased
its
levels
may
afford
antagonistic
effect
poisoning,
suggesting
potentially
effective
promote
Pb.
medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 23, 2023
Abstract
Background
Environmental
exposure
to
metal
mixtures
is
common
and
may
be
associated
with
increased
risk
for
neurodegenerative
disorders
including
Alzheimer’s
disease
Objective
This
study
examined
associations
of
mixed
exposures
medial
temporal
lobe
(MTL)
MRI
structural
metrics
neuropsychological
performance.
Methods
Metal
history,
whole
blood
metal,
tests
were
obtained
from
subjects
with/without
a
history
welding
fumes
(42
exposed
subjects;
31
controls).
MTL
structures
(hippocampus,
entorhinal
parahippocampal
cortices)
assessed
by
morphologic
(volume,
cortical
thickness)
diffusion
tensor
imaging
[mean
(MD),
axial
(AD),
radial
diffusivity
(RD),
fractional
anisotropy
(FA)]
metrics.
In
subjects,
correlation,
multiple
linear,
Bayesian
kernel
machine
regression,
mediation
analyses
employed
examine
effects
single-
or
mixed-metal
predictor(s)
their
interactions
on
metrics;
the
path
consequences.
Results
Compared
controls,
had
higher
Cu,
Fe,
K,
Mn,
Pb,
Se,
Zn
levels
(p’s<0.026)
poorer
performance
in
processing/psychomotor
speed,
executive,
visuospatial
domains
(p’s<0.046).
Exposed
displayed
MD,
AD,
RD
all
ROIs
(p’s<0.040)
lower
FA
cortices
(p’s<0.033),
but
not
morphological
differences.
Long-term
indirectly
predicted
processing
speed
via
(p=0.023).
Higher
Mn
Cu
(p’s<0.043)
Delayed
Story
Recall
(p=0.007)
without
overall
mixture
interaction
effects.
Discussion
Mixed
features
that
are
similar
at-risk
populations.
These
data
warrant
follow-up
as
they
illuminate
environmental
disease-related
health
outcomes.
Highlights
through
than
controls.
(DTI)
mean
(RD)
values
regions
interest
(ROI)
(FA)
significant
altered
DTI
(lower
hippocampal
RD).
Across
regression
analyses,
performance,
resembling
mediate,
at
least
partially,
cognitive
Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: July 20, 2022
Abstract
Lead
(Pb)
is
a
corrosion-resistant,
heavy,
non-ferrous
metal
with
widespread
environmental
pollution.
Several
chelators
have
been
used
in
the
treatment
of
Pb
poisoning.
However,
effect
sodium
para-aminosalicylic
acid（PAS-Na）on
excretion
has
yet
to
be
reported.
To
investigate
effects
PAS-Na
on
excretion,
mouse
model
acute
lead
exposure
was
established.
Healthy
mice
(90)
received
abdominal
injection
(i.p.)
120
mg/kg
acetate,
and
4
h
later
80,
160,
240
PAS-Na,
or
edetate
calcium
disodium
(CaNa
2
EDTA)
were
injected
subcutaneously
(s.c.)
once
per
day
for
6
days
into
corresponding
groups,
respectively.
The
Control,
Pb-exposed,
CaNa
EDTA
groups
evaluated
simultaneously.
After
collecting
24
urine
samples,
animals
sacrificed
batches
2nd,
4th,
6th
day.
Levels
other
elements
[including
manganese
(Mn)
copper
(Cu)]
urine,
whole
blood
brain
tissues
analyzed
by
graphite
furnace
atomic
absorption
spectrometry
(AAS).
results
showed
that
increased
levels
mice,
afforded
antagonistic
against
Pb-induced
toxicity.
While
additional
studies
will
needed,
these
novel
establish
as
potential
efficacious
mitigating
Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Oct. 4, 2022
Abstract
Lead
(Pb)
is
a
corrosion-resistant,
heavy,
non-ferrous
metal
with
widespread
environmental
pollution.
Several
chelators
have
been
used
in
the
treatment
of
Pb
poisoning.
However,
effect
sodium
para-aminosalicylic
acid(PAS-Na)on
excretion
has
yet
to
be
reported.
To
investigate
effects
PAS-Na
on
excretion,
mouse
model
acute
lead
exposure
was
established.
Healthy
mice
(90)
received
abdominal
injection
(i.p.)
120
mg/kg
acetate,
and
4
h
later
80,
160,
240
PAS-Na,
or
edetate
calcium
disodium
(CaNa
2
EDTA)
were
injected
subcutaneously
(s.c.)
once
per
day
for
6
days
into
corresponding
groups,
respectively.
The
Control,
Pb-exposed,
CaNa
EDTA
groups
evaluated
simultaneously.
After
collecting
24
urine
samples,
animals
sacrificed
batches
2nd,
4th,
6th
day.
Levels
other
elements
[including
manganese
(Mn)
copper
(Cu)]
urine,
whole
blood
brain
tissues
analyzed
by
graphite
furnace
atomic
absorption
spectrometry
(AAS).
results
showed
that
increased
levels
mice,
afforded
antagonistic
against
Pb-induced
toxicity.
While
additional
studies
will
needed,
these
novel
establish
as
potential
efficacious
mitigating
