Effects of Exosomes on Neurological Function Recovery for Ischemic Stroke in Pre-clinical Studies: A Meta-analysis DOI Creative Commons

Mudan Huang,

Zhongqiu Hong,

Chongjun Xiao

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: Nov. 26, 2020

Background: Exosomes, especially stem cell-derived exosomes, have been widely studied in pre-clinical research of ischemic stroke. However, their pooled effects remain inconclusive. Methods: Relevant literature concerning the exosomes on neurological performance a rodent model stroke was identified via searching electronic databases, including PubMed, Embase, and Web Science. The primary outcomes included function scores (NFS) infarct volume (IV), secondary were several pro-inflammatory factors terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling-positive cells. Subgroup analyses regarding potentially influencing NFS IV also conducted. Results: We 21 experiments from 18 studies meta-analysis. Pooled showed positive significant (standardized mean difference −2.79; 95% confidence interval −3.81 to −1.76) −3.16; −4.18 −2.15). Our data revealed that models might be related routes administration sources. In addition, there attenuation factors, interleukin-6, tumor necrosis factor-α interleukin-1β, cells when undergoing treatment. Conclusion: Cell-derived treatment demonstrated statistically improvements structural recovery animal results provide relatively robust evidence supporting as promising therapy promote individuals.

Language: Английский

Stem Cell-Derived Exosomes: a New Strategy of Neurodegenerative Disease Treatment DOI Open Access
Nashmin Fayazi Hosseini, Mohsen Sheykhhasan, Sara Soleimani Asl

et al.

Molecular Neurobiology, Journal Year: 2021, Volume and Issue: 58(7), P. 3494 - 3514

Published: March 21, 2021

Language: Английский

Citations

110
Exosome-mediated delivery and regulation in neurological disease progression DOI Creative Commons
Gurpreet Singh,

Ankit Mehra,

Sanchit Arora

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130728 - 130728

Published: March 11, 2024

Exosomes (EXOs), minute membranous structures originating from diverse biological sources, have recently seized the attention of researchers due to their theranostic potential for neurological diseases. Released actively by various cells, including stem adipose tissue, and immune EXOs wield substantial regulatory influence over intricate landscape complications, exhibiting both positive negative modulatory effects. In AD, play a pivotal role in disseminating breaking down amyloid-β protein. Moreover, derived mesenchymal cells showcase remarkable capacity mitigate pro-inflammatory phenotypes regulating miRNAs neurodegenerative These vesicles possess unique ability traverse blood-brain barrier, governing aggregation mutant huntingtin Understanding exosomal functions within CNS holds significant promise enhancing treatment efficacy This review intricately examines mechanisms involving disease development, highlighting therapeutic prospects exploring utility exosome-based nanomedicine complications. Additionally, delves into challenges associated with drug delivery brain, emphasizing complexities inherent this critical aspect neurotherapeutics.

Language: Английский

Citations

32
Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux DOI Creative Commons
Qing Zeng,

Yuqing Zhou,

Donghui Liang

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: July 17, 2020

Background: Cerebral ischemia-reperfusion (I/R) injury can lead to severe dysfunction, and its treatment is difficult. It reported that nucleotide-binding domain leucine-rich repeat family protein 3 (NLRP3) inflammasome-mediated cell pyroptosis an important part of cerebral I/R the activation autophagy inhibit in some tissue injury. Our previous study found protective effects bone marrow mesenchymal stem cells (BMSCs) may be associated with regulation autophagy. Recent studies have demonstrated exosomes secreted from BMSCs (BMSC-Exos) play essential role effective biological performance mechanism BMSC-Exos remission inflammation, but it has not been We aimed investigate on determine if autophagic flux. Method: PC12 were subjected oxygen-glucose deprivation/reoxygenation (OGD/R) induce vitro cocultured BMSC-Exos. Cell viability was determined CCK-8 lactate dehydrogenase (LDH) detection kits. Scanning electron microscopy (SEM), Hoechst 33342/propidium iodide (PI) double staining, 2',7'-dichlorodihydrofluorescein diacetate assay, immunofluorescence, Western blot, Enzyme-linked immunosorbent assay (ELISA) used detect pyroptosis. Furthermore, transmission (TEM), GFP-RFP-LC3 adenovirus transfection, blot flux influence Finally, coimmunoprecipitation binding interaction between NLRP3 LC3. Results: increased OGD/R. The inhibitory effect comparable inhibitor MCC950 reversed by overexpression. promoted through AMP-activated kinase (AMPK)/mammalian target rapamycin pathway, whereas chloroquine, AMPK silencing, compound C blocked Conclusions: protect against OGD/R via attenuation promoting AMPK-dependent

Language: Английский

Citations

74
Bone Marrow Mesenchymal Stem Cell-Derived Exosomal KLF4 Alleviated Ischemic Stroke Through Inhibiting N6-Methyladenosine Modification Level of Drp1 by Targeting lncRNA-ZFAS1 DOI

Qingsong Wang,

Rongjun Xiao,

Jun Peng

et al.

Molecular Neurobiology, Journal Year: 2023, Volume and Issue: 60(7), P. 3945 - 3962

Published: March 31, 2023

Language: Английский

Citations

26
Engineered extracellular vesicles as brain therapeutics DOI
Miguel Lino, Susana Simões, Francesca Tomatis

et al.

Journal of Controlled Release, Journal Year: 2021, Volume and Issue: 338, P. 472 - 485

Published: Aug. 21, 2021

Language: Английский

Citations

50
Challenges and Improvements of Novel Therapies for Ischemic Stroke DOI Creative Commons

Lijun Yang,

Jing Qian, Bo Yang

et al.

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Sept. 30, 2021

Stroke is the third most common disease all over world, which regarded as a hotspot in medical research because of its high mortality and morbidity. Stroke, especially ischemic stroke, causes severe neural cell death, no effective therapy currently available for neuroregeneration after stroke. Although many therapies have been shown to be preclinical studies almost none them passed clinical trials, reasons failures not well identified. In this review, we focus on several novel methods, such traditional Chinese medicine, stem therapy, exosomes that used stroke till recent decades. We summarize proposed basic mechanisms underlying these related results, discussing advantages current limitations each emphatically. Based side effects, narrow therapeutic window, less accumulation at injury region, structure transformation drug combination are subsequently applied, providing deep understanding develop treatment strategies near future.

Language: Английский

Citations

36
Dysfunction of the Neurovascular Unit in Ischemic Stroke: Highlights on microRNAs and Exosomes as Potential Biomarkers and Therapy DOI Open Access
Timea Forró, Zoltán Bajkó,

Adrian Bălașa

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(11), P. 5621 - 5621

Published: May 25, 2021

Ischemic stroke is a damaging cerebral vascular disease associated with high disability and mortality rates worldwide. In spite of the continuous development new diagnostic prognostic methods, early detection outcome prediction are often very difficult. The neurovascular unit (NVU) complex multicellular entity linking interactions between neurons, glial cells, brain vessels. Novel research has revealed that exosome-mediated transfer microRNAs plays an important role in cell-to-cell communication and, thus, integral crosstalk within NVU. After stroke, NVU homeostasis altered, which induces release several potential biomarkers into blood addition biological data representing all constituents to clinical neuroradiological findings can significantly advance evaluation prognosis. this review, we present current literature regarding possible beneficial roles exosomes derived from components multipotent mesenchymal stem cells preclinical studies ischemic stroke. We also discuss most relevant trials on patients.

Language: Английский

Citations

34
Extracellular Vesicles: Novel Roles in Neurological Disorders DOI Creative Commons
Qian Jin, Peipei Wu, Xinru Zhou

et al.

Stem Cells International, Journal Year: 2021, Volume and Issue: 2021, P. 1 - 16

Published: Feb. 17, 2021

Exosomes are small extracellular vesicles (EVs) secreted by almost all cells, which have been recognized as a novel platform for intercellular communication in the central nervous system (CNS). capable of transferring proteins, nucleic acids, lipids, and metabolites between neurons glial contributing to CNS development maintenance homeostasis. Evidence shows that exosomes originating from cells act suppressors or promoters initiation progression neurological disorders. Moreover, these shown transfer molecules associated with diseases through blood-brain barrier (BBB) thus can be detected blood. This unique feature enables potential diagnostic biomarkers In addition, substantial number researches indicated derived mesenchymal stem (MSCs) repair effects on Herein, we briefly introduce roles under physiological pathological conditions. particular, therapeutic tools disorders highlighted.

Language: Английский

Citations

33
Focus on the role of mitochondria in NLRP3 inflammasome activation: A prospective target for the treatment of ischemic stroke (Review) DOI Creative Commons
Xiaolu Zhang, Wenyun Zeng, Yue Zhang

et al.

International Journal of Molecular Medicine, Journal Year: 2022, Volume and Issue: 49(6)

Published: April 7, 2022

Post‑ischemic neuroinflammation induced by the innate local immune response is a major pathophysiological feature of cerebral ischemic stroke, which remains leading cause mortality and disability worldwide. NLR family pyrin domain containing (NLRP)3 inflammasome crucially mediates post‑ischemic inflammatory responses via its priming, activation interleukin‑1β release during hypoxic‑ischemic brain damage. Mitochondrial dysfunctions are among main hallmarks several diseases, including stroke. In present review, focus was addressed on role mitochondria in stroke while keeping NLRP3 as link. Under ischemia hypoxia, capable controlling inflammasome‑mediated through mitochondrial released contents, localization related proteins. Thus, may be attractive targets to treat well drugs that target process function At present, certain have already been studied clinical trials.

Language: Английский

Citations

26
Combination of Stem Cells and Rehabilitation Therapies for Ischemic Stroke DOI Creative Commons

Reed Berlet,

Stefan Anthony,

Beverly Brooks

et al.

Biomolecules, Journal Year: 2021, Volume and Issue: 11(9), P. 1316 - 1316

Published: Sept. 6, 2021

Stem cell transplantation with rehabilitation therapy presents an effective stroke treatment. Here, we discuss current breakthroughs in stem research along strategies that may have a synergistic outcome when combined together after stroke. Indeed, offers promising new approach and add to therapies. By reviewing the pathophysiology of mechanisms by which cells attenuate this inflammatory process, hypothesize will provide better functional outcomes for patients. Using preclinical data, explore prominent types cells, existing theories repair, treatments inside brain, modalities outside evidence pertaining benefits therapy. In review article, assess advantages disadvantages using mitigate devastating effects

Language: Английский

Citations

32