BioSolutions for Green Agriculture: Unveiling the Diverse Roles of Plant Growth‐Promoting Rhizobacteria

International Journal of Microbiology, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The extensive use of chemical pesticides and fertilizers in conventional agriculture has raised significant environmental health issues, including the emergence resistant pests pathogens. Plant growth-promoting rhizobacteria (PGPR) present a sustainable alternative, offering dual benefits as biofertilizers biocontrol agents. This review delves into mechanisms by which PGPR enhance plant growth, nutrient solubilization, phytohormone production, pathogen suppression. PGPR's commercial viability application, particularly under abiotic stress conditions, are also examined. improves growth directly enhancing uptake producing substances indirectly inhibiting phytopathogens through such siderophore production secretion lytic enzymes. Despite their potential, commercialization faces challenges, strain specificity, formulation stability, regulatory barriers. highlights need for ongoing research to deepen our understanding plant-microbe interactions develop more robust formulations. Addressing these challenges will be crucial integrating mainstream agricultural practices reducing reliance on synthetic agrochemicals. successful adoption could lead practices, promoting healthier crops ecosystems.

Language: Английский

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A novel biocontrol agent Bacillus velezensis K01 for management of gray mold caused by Botrytis cinerea

AMB Express, Journal Year: 2023, Volume and Issue: 13(1)

Published: Aug. 29, 2023

Gray mold is a destructive plant disease caused by fungal pathogen Botrytis cinerea. The use of growth promoting rhizobacteria (PGPR) has proven to be promising method control this disease. Bacillus velezensis K01 was isolated from the rhizosphere planting tomatoes. Strain range roles, including ability solubilize phytate phosphorus, stimulate resistant response, and produce indoleacetic acid (IAA), protease, cellulase, antimicrobial substances. found inhibit 12 phytopathogenic fungi 5 bacteria. Specially, strain demonstrated biocontrol efficiency over 78% against gray B. cinerea on leaves fruits tomato pepper. Additionally, promote maize seedlings. Further genomic analysis revealed that belongs velezensis, which consistent with phylogenetic analysis, average nucleotide polymorphism (ANI), digital DNA-DNA hybridization (dDDH). genome had size 3,927,799 bp deduced 3866 predicted genes, an guanine-cytosine (GC) content 46.5%. Based analyses secondary metabolites, 18.4% annotated gene clusters related metabolite synthesis. annotation comparative genomics identified several genes associated promotion environmental adaption. These findings suggest potential serve as new agent for management

Language: Английский

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Emulsifying Properties of Rhamnolipids and Their In Vitro Antifungal Activity against Plant Pathogenic Fungi

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7746 - 7746

Published: Nov. 10, 2022

Rhamnolipids have significant emulsifying activity and the potential to become a component of pesticide emulsifier. are usually composed two main components: mono-rhamnolipids (Rha-C10-C10) di-rhamnolipids (Rha2-C10-C10). The proportion in products ranged between 15% 90%, affected by production strains fermentation process. In this paper, three kinds rhamnolipid containing proportions, 25.45, 46.46 89.52%, were used test their ability toward conventional solvents (S-200, xylene, cyclohexanone) antifungal activities against five plant pathogenic fungi (Phytophthora capsici, Phytophthora parasitica var.nicotianae, Colletotrichum destructivum, sublineolum, Fusarium oxysporum). results indicated that although CMC rhamnolipids significantly different, emulsification properties had no remarkable differences, at concentration 10 g/L. However, different: more di-rhamnolipids, stronger activity. This work helps promote application as pesticides adjuvants.

Language: Английский

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Enhancing plant defense using rhizobacteria in processing tomatoes: a bioprospecting approach to overcoming Early Blight and Alternaria toxins

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 4, 2023

Plant growth-promoting rhizobacteria (PGPR) with antagonistic activity toward plant pathogenic fungi are valuable candidates for the development of novel protection products based on biocontrol activity. The very first step in formulation such is to screen potential effectiveness selected microorganism(s). In this study, non-pathogenic were isolated from rhizosphere tomato plants and evaluated their against three species mycotoxin-producing Alternaria. assessment involved investigating both fungal biomass Alternaria toxin reduction. A ranking system developed allowed identification 12 best-performing strains among initial 85 isolates. Several showed a significant reduction (up 76%) and/or mycotoxin production 99.7%). Moreover, same isolates also demonstrated (PGP) traits as siderophore or IAA production, inorganic phosphate solubilization, nitrogen fixation, confirming multifaceted properties PGPRs. Bacillus species, particularly B. amyloliquefaciens two subtilis, highest efficacy reducing effective lowering production. Isolates Enterobacter ludwigii, asburiae, Serratia nematodiphila, Pantoea agglomerans, Kosakonia cowanii moderate efficacy. Results suggest that by leveraging diverse capabilities different microbial strains, consortium-based approach would provide broader spectrum effectiveness, thereby signaling more encouraging resolution sustainable agriculture addressing nature crop-related biotic challenges.

Language: Английский

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Novel indan-1,3-dione derivatives: Design, green synthesis, effect against tomato damping-off disease caused by Fusarium oxysporum and in silico molecular docking study

Arabian Journal of Chemistry, Journal Year: 2022, Volume and Issue: 15(5), P. 103731 - 103731

Published: Feb. 3, 2022

An effective method for synthesizing series of twenty-two new compounds 1, 2a,b, 3, 4a,b, 5a-e, 7, 8, 9, 10, 12, 13a-d, 15a,b was performed starting from reaction 1,2,3-indenetrione thiourea, and ethyl cyanoacetate under microwave irradiation / or 2-(1,3-dioxo-1H-inden-2(3H)-ylidene) hydrazine carbothioamide with acetic anhydride. Chemical structure the obtained products has been established by spectroscopic techniques including FTIR, 1H NMR, 13C DEPT-135, mass spectroscopy. The designed have successfully examined in-vitro their antifungal activities. relation between synthesized activity against tomato damping-off disease caused Fusarium oxysporum fungi studied favourable results were obtained. studies indicated that 4a 5a-d exerted highest activities, while 3 4b recorded lowest effect. confirmed possibility application 6′-amino-1,3-dioxo-2′-thioxo-1,2′,3,3′-tetrahydro-1′H-spiro[indene-2,4′-pyrimidine]-5′-carboxylate 1 as a regulator vegetative growth tomatoes. molecular docking analysis within succinate dehydrogenase (SDH) target enzyme in order to rationalize promising findings active 5a-d, 13a.

Language: Английский

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Integrated management of tomato gray mold disease using selective plant extracts and rhizobacteria

Plant Protection, Journal Year: 2024, Volume and Issue: 8(4), P. 781 - 787

Published: Dec. 29, 2024

Botrytis cinerea is one of the most significant plant pathogens globally, causing extensive damage to fruits and vegetables. Botrycides remain a widely used method protection; however, introduction plant-beneficial bacteria offers promising agroecologically sustainable alternative. The current study evaluated effectiveness extracts rhizobacteria in controlling diseases caused by B. tomatoes. Neem (Azadirachta indica), garlic (Allium sativum), turmeric (Curcuma longa) were tested alongside rhizobacterial strains (Bacillus subtilis, Pseudomonas fluorescens, Trichoderma harzianum). In vitro experiments showed that, at 15% extract concentration, neem inhibited fungal growth 78% 72%, respectively, while subtilis suppressed mycelial 62%. Synergistic treatments, particularly combined with exhibited highest inhibition zone (22 mm) an 85% synergy effect. Greenhouse trials demonstrated that significantly reduced disease severity, achieving severity index (DSI) 1.3 75% reduction compared controls, which had DSI 4.8. alone 52.1% 58.3%, respectively. combination proved more effective managing grey mold, promoting healthier plants, providing potential chemical-free alternative fungicides. Further field are recommended develop scalable, eco-friendly solutions for tomato management.

Language: Английский

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Fungicidal and plant growth-promoting traits of Lasiodiplodia pseudotheobromae, an endophyte from Andrographis paniculata

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: June 14, 2023

Fungal endophytes are widespread and dwell inside plant cells for at least part of their life without producing any symptoms infection. Distinct host plants may have different fungal endophyte rates community compositions. Despite this, the endophytic fungi connected with hostile behaviors, remain unknown.The objective current research was to isolate identify species from root Andrographis paniculata. The effects APR5 on mycelial growth phytopathogens production plant-promoting traits were assessed.Endophytic showed higher inhibitory efficacy in dual double plate assay against tested phytopathogenic fungi. scanning electron microscope analysis demonstrated that hyphae coiled by which makes them shrink disintegrate. Further, an ethyl acetate crude extract effectively suppressed mycelium Rhizoctonia solani 75 ± 0.1% agar well diffusion assay. identified as Lasiodiplodia pseudotheobromae using nuclear ribosomal DNA internal transcribed spacer (ITS) region qualitatively evaluated capacity produce growth-promoting hormones. Gas chromatography-mass spectrometry implemented acquire a preliminary understanding secondary metabolic profile extract. 1-octadecene, erythritol, niacin, oleic acid, phenol, pantolactone, phenyl alcohol, p-cresol, tbutyl hydroquinone metabolites analyzed reported antimicrobial properties.

Language: Английский

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Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato (Solanum lycopersicum var. cerasiforme)

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 7, 2023

Gray mold, caused by Botrytis cinerea is a major cause of post-harvest rot fresh fruits and vegetables. The utilization selected microorganisms as biocontrol agents promising alternative to effectively control gray mold on tomatoes. current study was conducted explore potential mechanisms the Pseudomonas strain infections Among 8 tested bacterial isolates, protegens ML15 demonstrated antagonistic activity . Moreover, P. exhibited production siderophores, hydrogen cyanide, ammonia, exopolysaccharides, lipase, biosurfactant, 2,4-diacetylphloroglucinol, several other antifungal compounds, such 1-tetradecanol, cyclododecane, 2,4-di-tert-butylphenol, 2-methyl-1-hexadecanol. A comprehensive genomic analysis unravels 18 distinct genetic regions with for biosynthesizing secondary metabolites, known their pivotal role in responses against plant pathogens. In vivo , experiments showed that both culture suspension cell-free supernatant significantly reduced fungal growth (53.0 ± 0.63%) mitigated disease development (52.8 1.5%) cherry tomatoes at four days post- B inoculation. During infection, application resulted augmentation total antioxidant, phenolic content, ascorbic acids content. Thus, our results suggested ML15’s agent B. -induced postharvest tomato decay achieved through secretion substances, induction defense responses, inhibition mycelial These findings provide significant contribution ongoing search alternative, eco-friendly methods controlling products. could help reduce reliance chemical fungicides promote sustainable agriculture practices.

Language: Английский

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Efficiency and mechanisms of action of pelletized compost loaded with Bacillus velezensis CE 100 for controlling tomato Fusarium wilt

Biological Control, Journal Year: 2022, Volume and Issue: 176, P. 105088 - 105088

Published: Oct. 19, 2022

Language: Английский

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The Effect of Plant Growth Promoting Rhizobacteria and Chemical Fertilizers on Tomato Cultivation

Tekirdağ Ziraat Fakültesi Dergisi, Journal Year: 2024, Volume and Issue: 21(2), P. 416 - 428

Published: March 3, 2024

Mevcut çalışmada, örtü altı domates yetiştiriciliğinde bitki gelişimini teşvik eden kök bakterileri ve kimyasal gübre uygulamalarının domatesin bazı verim kalite özelliklerine etkisi araştırılmıştır. Çalışmada yer alan bakteriler bakteri I (Stenotrophomonas maltophilia strain BY-44, Bacillus licheniformis HK-13 Pseudomonas putida NK-12) II (Pseudomonas fluorescens FC-42, subtilis SK-26 Rhizobium radiobacter SK-63) uygulaması olmak üzere iki farklı grup olarak uygulanmıştır. Kimyasal programına paralel bitkilere azot içerikli (NPK) verildiğinde uygulamaları için fikse edebilme özelliğine sahip NK-12 strainleri, fosfor saf gübreler çözebilme özellikleri pozitif olan BY-44 SK-63 potasyum oranı yüksek NPK gübrelerin uygulandığı dönemde ise potasyumu çözebilen FC-42 strainleri karıştırılmadan tek inokule edilmiştir. Deneme, 2017 yılında Hasyurt’da (Finike-Antalya) bulunan plastik serada, tesadüf parselleri deneme desenine göre kurulmuş 3 tekerrürlü yürütülmüştür. uygulamaların domateste salkım sayısı, birikimli meyve boyu, çapı, sertliği, ortalama ağırlığı, erkenci verim, kuru toplam suda çözünebilir madde, suyunun pH değeri titre edilebilir asit miktarına değerlendirilmiştir. Yapılan uygulamalar sonucunda değeri, ağırlığı sertliği açısından ile arasında istatistiki önemli bir farklılık olmadığı belirlenmiştir. Bakteri uygulamasının boyuna etkisinin aynı olduğu, de uygulamasıyla grupta aldığı görülmüştür. Birikimli parametrelerinde en değerlerin uygulamasından elde edildiği Ayrıca uygulamalarda kullanılan stainlerinin IAA, siderofor, ACC-deaminaz katalaz gibi mekanizmaları En IAA HK-13’de (61.24µg ml-1), siderofor üretimi NK-12’de (36 mm) bulunmuştur. Strainlerin hepsinin ACC- deaminaz enzimine olduğu tespit birçok parametrede veya yakın sonuç vermesi uygulamalarına verilmesinin kullanımının azaltılmasında etkili olacağını göstermiştir.

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