Making Waves: New Developments in Toxicology With the Zebrafish

Toxicological Sciences, Journal Year: 2018, Volume and Issue: 163(1), P. 5 - 12

Published: Feb. 16, 2018

The laboratory zebrafish (Danio rerio) is now an accepted model in toxicologic research. fills a niche between vitro models and mammalian biomedical models. developmental characteristics of the small fish are strategically being used by scientists to study topics ranging from high-throughput toxicity screens multi- transgenerational studies. High-throughput technology has increased utility embryonic assays screening chemicals drugs for or effect. Additionally, advances behavioral characterization experimental methodology allow observation recognizable phenotypic changes after xenobiotic exposure. Future directions research predicted take advantage CRISPR-Cas9 genome editing methods creating disease interrogating mechanisms action with fluorescent reporters tagged proteins. Zebrafish can also origins health toxicity. many advantages as new methodologies areas continue expand usefulness application zebrafish.

Language: Английский

---

Metabolomic profiling reveals the intestinal toxicity of different length of microplastic fibers on zebrafish (Danio rerio)

Journal of Hazardous Materials, Journal Year: 2020, Volume and Issue: 403, P. 123663 - 123663

Published: Aug. 13, 2020

Language: Английский

---

Zebrafish: Development of a Vertebrate Model Organism

Current Protocols Essential Laboratory Techniques, Journal Year: 2018, Volume and Issue: 16(1)

Published: May 1, 2018

Abstract Zebrafish ( Danio rerio ) are a small‐bodied tropical, freshwater fish species originally from South Asia. The ease of care, year‐round prolific breeding, and transparent, external development have made these popular model vertebrate for many fields biology. Over the past 40 years, methods genetic developmental manipulation driven rapid rise in their use research. This review provides an orientation to zebrafish as system, its ecology life history early research adoption key development. Its current uses techniques that highlight future areas system also discussed. Finally, introduction essentials care presented, highlighting this teaching use. © 2018 by John Wiley & Sons, Inc.

Language: Английский

---

Zebrafish as a Successful Animal Model for Screening Toxicity of Medicinal Plants

Plants, Journal Year: 2020, Volume and Issue: 9(10), P. 1345 - 1345

Published: Oct. 12, 2020

The zebrafish (Danio rerio) is used as an embryonic and larval model to perform in vitro experiments developmental toxicity studies. Zebrafish may be determine the of samples early screening assays, often a high-throughput manner. embryotoxicity at leading edge toxicology research due short time required for analyses, transparency embryos, life cycle, high fertility, genetic data similarity. studies range from assessing bioactive compounds or crude extracts plants determining optimal process. Most studied were polar, such ethanol, methanol, aqueous solutions, which detect bioactivity. This review examines latest using study highlights its power tool detecting medicinal effectiveness enhancing understanding new drug generation. goal this was develop link ethnopharmacological that can by other researchers conduct future research.

Language: Английский

---

Adverse effects polystyrene microplastics exert on zebrafish heart – Molecular to individual level

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 416, P. 125969 - 125969

Published: April 29, 2021

Language: Английский

---

Polymer pollution and its solutions with special emphasis on Poly (butylene adipate terephthalate (PBAT))

Polymer Bulletin, Journal Year: 2022, Volume and Issue: 79(11), P. 9303 - 9330

Published: Jan. 6, 2022

Language: Английский

---

A mechanistic understanding of the effects of polyethylene terephthalate nanoplastics in the zebrafish (Danio rerio) embryo

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 2, 2023

Plastic pollution, especially by nanoplastics (NPs), has become an emerging topic due to the widespread existence and accumulation in environment. The research on bioaccumulation toxicity mechanism of NPs from polyethylene terephthalate (PET), which is widely used for packaging material, have been poorly investigated. Herein, we report first use high-resolution magic-angle spinning (HRMAS) NMR based metabolomics combination with assay behavioural end points get systems-level understanding PET intact zebrafish embryos. exhibited significant alterations hatching survival rate. Accumulation larvae were observed liver, intestine, kidney, coincide localization reactive oxygen species these areas. HRMAS data reveal that cause: (1) alteration metabolites related targeting liver pathways associated detoxification oxidative stress; (2) impairment mitochondrial membrane integrity as reflected elevated levels polar head groups phospholipids; (3) cellular bioenergetics evidenced changes numerous interrelated energy metabolism. Taken together, this work provides time a comprehensive system level exposure larvae.

Language: Английский

---

The posterity of Zebrafish in paradigm of in vivo molecular toxicological profiling

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 171, P. 116160 - 116160

Published: Jan. 21, 2024

The aggrandised advancement in utility of advanced day-to-day materials and nanomaterials has raised serious concern on their biocompatibility with human other biotic members. In last few decades, understanding toxicity these been given the centre stage research using many vitro vivo models. Zebrafish (Danio rerio), a freshwater fish member minnow family garnered much attention due to its distinct features, which make it an important frequently used animal model various fields embryology toxicological studies. Given that fertilization development zebrafish eggs take place externally, they serve as excellent organism for studying early developmental stages. Moreover, possess comparable genetic composition humans share almost 70% genes mammals. This particular become increasingly popular, especially research. serves link between studies analysis It is appealing choice vertebrate research, when employing high-throughput methods, small size, swift development, relatively affordable laboratory setup. enhanced comprehension pathobiology drug toxicity. review emphasizes recent developments screening assays, new insights gained about drugs through assays. Specifically, cardio, neural, and, hepatic toxicology inferred by applications nanoparticles have highlighted.

Language: Английский

---

Toxicity profiling of flame retardants in zebrafish embryos using a battery of assays for developmental toxicity, neurotoxicity, cardiotoxicity and hepatotoxicity toward human relevance

Neurotoxicology and Teratology, Journal Year: 2018, Volume and Issue: 70, P. 40 - 50

Published: Oct. 9, 2018

Following the voluntary phase-out of brominated flame retardants (BFRs) due to their environmental persistence and toxicity, organophosphorus (OPFRs) are emerging replacements. However, there is limited information on potential human health effects OPFRs. Zebrafish embryos a viable vertebrate model organism with many advantages for high throughput testing toward hazard assessment. We utilized zebrafish assess developmental neurotoxicity, cardiotoxicity hepatotoxicity, eight replacement OPFRs: (triphenyl phosphate [TPHP], isopropylated phenyl [IPP], 2-ethylhexyl diphenyl [EHDP], tert-butylated [BPDP], trimethyl [TMPP], isodecyl [IDDP], tris(1,3-dichloroisopropyl) [TDCIPP], tris(2-chloroethyl) [TCEP]) two BFRs (3,3',5,5'- tetrabromobisphenol A [TBBPA] 2,2'4,4'-brominated ether [BDE-47]). To determine teratogenicity, were exposed (FRs) at 4 h post fertilization (hpf) days (dpf) morphological alterations corresponding survival evaluated 2 dpf. Internal concentrations measured in larvae used this assay by liquid chromatography-mass spectrometry. Locomotor activity was assessed treated 48 (from 3 dpf 5 dpf), followed hepatotoxicity evaluation. Finally, heart rate rhythmicity hpf compounds h. Results suggest that several OPFRs (BPDP, EHDP; IPP, TMPP; TPHP TDCIPP) produced adverse multiple target organs comparable BFRs. As these have capacity disrupt an integrated model, they potentially affect mammalian biology. Then, we compared lowest effective levels (LEL) estimated or plasma using biomonitoring data (human plasma, breast milk, handwipe samples house dust) toxicokinetic (HTTK) model. indicate some compounds, nominal LELs within range exposures, while internal above exposures humans. These findings demonstrate value as relevant screening tool support need further characterization

Language: Английский

---

Building and Applying Quantitative Adverse Outcome Pathway Models for Chemical Hazard and Risk Assessment

Environmental Toxicology and Chemistry, Journal Year: 2019, Volume and Issue: 38(9), P. 1850 - 1865

Published: May 25, 2019

Abstract An important goal in toxicology is the development of new ways to increase speed, accuracy, and applicability chemical hazard risk assessment approaches. A promising route integration vitro assays with biological pathway information. We examined how adverse outcome (AOP) framework can be used develop pathway‐based quantitative models useful for regulatory safety assessment. By using AOPs as initial conceptual AOP knowledge base a source data on key event relationships, different methods applied computational (qAOPs) relevant decision making. qAOP model may not necessarily have same structure it based on. Useful modeling range from statistical, Bayesian networks, regression, ordinary differential equations individual‐based should chosen according questions being asked available. discuss need toxicokinetic provide linkages between exposure qAOPs, extrapolate vivo, across species. Finally, we identify best practices building necessity transparent comprehensive documentation gain confidence use ultimately their applications. Environ Toxicol Chem 2019;38:1850–1865. © 2019 The Authors. Environmental Toxicology Chemistry published by Wiley Periodicals, Inc. behalf SETAC.

Language: Английский

---