Magnesium-based biomaterials as emerging agents for bone repair and regeneration: from mechanism to application

Journal of Magnesium and Alloys, Journal Year: 2021, Volume and Issue: 9(3), P. 779 - 804

Published: April 6, 2021

Magnesium (Mg) is the fourth most abundant element in human body and important terms of specific osteogenesis functions. Here, we provide a comprehensive review use magnesium-based biomaterials (MBs) bone reconstruction. We history MBs their excellent biocompatibility, biodegradability osteopromotive properties, highlighting them as candidates for new generation biodegradable orthopedic implants. In particular, results reported field-specific literature (280 articles) recent decades are dissected with respect to extensive variety applications, including Mg/Mg alloys, bioglasses, bioceramics, polymer materials. also summarize osteogenic mechanism MBs, detailed section on physiological process, namely, enhanced osteogenesis, promotion osteoblast adhesion motility, immunomodulation, angiogenesis. Moreover, merits limitations current grafts substitutes compared. The objective this reveal strong potential agents repair regeneration highlight issues that impede clinical translation. Finally, development challenges transplanted materials discussed.

Language: Английский

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Recent advances in PLGA-based biomaterials for bone tissue regeneration

Acta Biomaterialia, Journal Year: 2021, Volume and Issue: 127, P. 56 - 79

Published: April 6, 2021

Language: Английский

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Magnetic Hyperthermia–Synergistic H₂O₂ Self‐Sufficient Catalytic Suppression of Osteosarcoma with Enhanced Bone‐Regeneration Bioactivity by 3D‐Printing Composite Scaffolds

Advanced Functional Materials, Journal Year: 2019, Volume and Issue: 30(4)

Published: Nov. 4, 2019

Abstract Chemotherapy resistance and bone defects caused by surgical excision of osteosarcoma have been formidable challenges for clinical treatment. Although recently developed nanocatalysts based on Fenton‐like reactions catalytic therapy demonstrate high potential to eliminate chemotherapeutic‐insensitive tumors, insufficient concentration intrinsic hydrogen peroxide (H 2 O ) low intratumoral penetrability hinder their applications therapeutic efficiency. The synchronous enriching intratumor H amount or nanoagents promoting osteogenesis are intriguing strategies solve the dilemma in therapy. Herein, a multifunctional “all‐in‐one” biomaterial platform is constructed co‐loading calcium (CaO iron oxide (Fe 3 4 nanoparticles into three‐dimensional (3D) printing akermanite scaffold (AKT‐Fe ‐CaO ). loaded CaO act as sources achieve self‐sufficient nanocatalytic catalyzed coloaded Fe nanoagents, well provide ion (Ca 2+ pools enhance regeneration. synergistic osteosarcoma‐therapeutic effect achieved from both magnetic hyperthermia as‐enabled under alternative fields hyperthermia‐enhanced reaction producing highly toxic hydroxyl radicals. Importantly, 3D AKT‐Fe composite scaffolds featured with favorable bone‐regeneration activity, providing worthy base positive enlightenment future treatment platforms.

Language: Английский

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Injectable GelMA Cryogel Microspheres for Modularized Cell Delivery and Potential Vascularized Bone Regeneration

Small, Journal Year: 2021, Volume and Issue: 17(11)

Published: Feb. 23, 2021

Abstract Cell therapeutics hold tremendous regenerative potential and the therapeutic effect depends on effective delivery of cells. However, current cell carriers with unsuitable cytocompatibility topological structure demonstrate poor viability during injection. Therefore, porous shape‐memory cryogel microspheres (CMS) are prepared from methacrylated gelatin (GelMA) by combining an emulsion technique gradient‐cooling cryogelation. Pore sizes CMS adjusted via procedure, optimized pore size (15.5 ± 6.0 µm) being achieved 30‐min gradient‐cooled variant (CMS‐30). Unlike hydrogel (HMS), promotes human bone marrow stromal (hBMSC) umbilical vein endothelial (HUVEC) adhesion, proliferated high levels stemness for 7 d, protects cells injection process using a 26G syringe needle. Moreover, CMS‐30 enhances osteogenic differentiation hBMSCs in osteoinductive media. can serve as building blocks delivering multiple types. Here, hBMSC‐loaded HUVEC‐loaded CMS‐30, mixed at 1:1 ratio, injected subcutaneously into nude mice 2 months. Results show development vascularized bone‐like tissue OCN CD31. These findings indicate that GelMA certain effectively deliver to achieve functional regeneration.

Language: Английский

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Metal-Based Nanostructures/PLGA Nanocomposites: Antimicrobial Activity, Cytotoxicity, and Their Biomedical Applications

ACS Applied Materials & Interfaces, Journal Year: 2019, Volume and Issue: 12(3), P. 3279 - 3300

Published: Dec. 24, 2019

Among the different synthetic polymers developed for biomedical applications, poly(lactic-co-glycolic acid) (PLGA) has attracted considerable attention because of its excellent biocompatibility and biodegradability. Nanocomposites based on PLGA metal-based nanostructures (MNSs) have been employed extensively as an efficient strategy to improve structural functional properties polymer. The MNSs used impart new PLGA, such antimicrobial labeling. In present review, strategies available fabrication MNS/PLGA nanocomposites their applications in field will be discussed, beginning with a description preparation routes, activity, cytotoxicity concerns nanocomposites. these nanocomposites, carriers scaffolds tissue regeneration other therapies are subsequently reviewed. addition, potential advantages using treatment illnesses analyzed vitro vivo studies, support future research field.

Language: Английский

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Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 14(6), P. 7592 - 7608

Published: Feb. 4, 2022

Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP magnesium NPs circumvent these drawbacks of chitosan-based hydrogels. Water-soluble deviate was first by grafting methacrylic anhydride phosphocreatine chain one-step lyophilization process. The this not only provides sites combine with binding but also serves as the reservoir control Mg2+ release. As result, lyophilized CSMP-MgO presented porous structure some small holes pore wall, diameters ranged from 50 100 μm. restricted swelling DI (lowest ratio 16.0 ± 1.1 g/g) no brittle failure during compression even at strain above 85% (maximum compressive strength 195.0 kPa) versus (28.0 41.3 kPa (0.5) hydrogels), regulated release stable sustained manner. promoted vitro calcium (hydroxyapatite (HA) tetracalcium (TTCP)) deposition supersaturated cytotoxicity MC3T3-E1 cells; cell osteogenic differentiation upregulation BSP, OPN, Osterix gene expression mineralization HUVEC tube formation. Among them, (5) most properties. Moreover, (CSMP-MgO (5)) showed excellent ability promote new formation calvarial defects rats. Thus, shows promise regeneration.

Language: Английский

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Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering

ACS Biomaterials Science & Engineering, Journal Year: 2022, Volume and Issue: 8(6), P. 2321 - 2335

Published: May 31, 2022

Large-sized bone defects are a great challenge in clinics and considerably impair the quality of patients' daily life. Tissue engineering strategies using cells, scaffolds, bioactive molecules to regulate microenvironment regeneration is promising approach. Zinc, magnesium, iron ions natural elements tissue participate many physiological processes metabolism therefore have potential for regeneration. In this review, we performed systematic analysis on effects zinc, engineering. We focus role these properties scaffolds (mechanical strength, degradation, osteogenesis, antibacterial properties, etc.). hope that our summary current research achievements notifications improve repair will find new inspiration breakthroughs inspire future research.

Language: Английский

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Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration

Bioactive Materials, Journal Year: 2022, Volume and Issue: 14, P. 377 - 388

Published: Feb. 1, 2022

Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, corresponding (H-Exo) exhibited superior in promoting cellular angiogenesis osteogenesis significant up-regulation focal adhesion, VEGF signaling pathway, thyroid hormone synthesis. Then, developed a platform technology enabling effective with sustained release kinetics to irregular-shaped defects injection. This is based on simple adsorbing technique, where adsorbed onto surface injectable porous poly(lactide-co-glycolide) (PLGA) microspheres bioinspired polydopamine (PDA) coating (PMS-PDA microspheres). The PMS-PDA effectively adsorb exosomes, show H-Exo 21 days high bioactivity, vascularized regeneration 5-mm rat calvarial defect. These findings indicate microsphere-based exosome efficient inducing regeneration, hence facilitating clinical translation exosome-based therapy.

Language: Английский

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Functional microspheres for tissue regeneration

Bioactive Materials, Journal Year: 2022, Volume and Issue: 25, P. 485 - 499

Published: Aug. 9, 2022

As a new type of injectable biomaterials, functional microspheres have attracted increasing attention in tissue regeneration because they possess some advantageous properties compared to other including hydrogels. A variety bio-inspired with unique structures and been developed as cellular carriers drug delivery vehicles recent years. In this review, we provide comprehensive summary the progress biodegradable that used for over last two decades. First, briefly introduce biomaterials general methods microsphere fabrication. Next, focus on newly technologies preparing microspheres, macroporous nanofibrous hollow core-shell structured surface-modified microspheres. After that, discuss application regeneration, specifically bone, cartilage, dental, neural, cardiac, skin regeneration. Last, present our perspectives future directions advancement

Language: Английский

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Magnesium Gradient‐Based Hierarchical Scaffold for Dual‐Lineage Regeneration of Osteochondral Defect

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(43)

Published: July 2, 2023

Abstract Osteochondral regeneration remains a great challenge due to the limited self‐healing ability and complexity of its hierarchical structure composition. Mg 2+ hypoxia are two effective modulators in boosting chondrogenesis. To this end, double‐layered scaffold (D) consisting hydrogel layer on porous cryogel is fabricated mimic osteochondral tissue. An gradient incorporated into with hypoxia‐mimicking deferoxamine (DFO) embedded (D‐Mg‐DFO), which remarkably augments dual‐lineage both cartilage subchondral bone. The higher supplementation from upper hydrogel, associated situation small pore size, exhibits promotive effects chondrogenic differentiation. lower bottom cryogel, interconnected macroporous structure, achieves multiple contributions stem cell migration bone marrow cavity, matrix mineralization, osteogenesis. Furthermore, rabbits’ trochlea defects established evaluate regenerative outcome. Compared control scaffolds containing only or DFO, D‐Mg‐DFO presents best effect under synergistic contribution factors. Overall, work provides new design toward an repair defect.

Language: Английский

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