Mussel inspired 3D elastomer enabled rapid calvarial bone regeneration through recruiting more osteoprogenitors from the dura mater DOI Creative Commons

Xuqiao Wang,

Chaoqun Ma, Xinchi Zhang

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

Abstract Currently, the successful healing of critical-sized calvarial bone defects remains a considerable challenge. The immune response plays key role in regulating regeneration after material grafting. Previous studies mainly focused on relationship between macrophages and marrow mesenchymal stem cells (BMSCs), while dural were recently found to play vital healing. In this study, series 3D elastomers with different proportions polycaprolactone (PCL) poly(glycerol sebacate) (PGS) fabricated, which further supplemented polydopamine (PDA) coating. physicochemical properties PCL/PGS PCL/PGS/PDA grafts measured, then they implanted as filling materials for 8 mm defects. results showed that matched effective PDA interface formed well-proportioned elastomer, effectively modulated polarization M2 promoted recruitment achieve full-thickness repair through both intramembranous endochondral ossification. Single-cell RNA sequencing analysis revealed predominance during their close macrophages. findings illustrated crosstalk determined vertical first time, may be new target designing

Language: Английский

Metformin enhances osteogenic differentiation of BMSC by modulating macrophage M2 polarization DOI Creative Commons

Siyu Fan,

Cunliang Zhang, Xin Sun

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 31, 2024

Language: Английский

Citations

3
Biomimetic Fabrication and Osteogenic Effects of E7BMP-2 Peptide Coassembly Microspheres Based α-Tricalcium Phosphate with Silk Fibroin DOI
Yan Zhu, Xin Yu,

Mingxuan Hao

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

The repair and reconstruction of bone defects remain a challenge in orthopedics. Inadequate mechanical qualities, poor biocompatibility, insufficient osteoconductivity are some the issues facing current healing materials. Better materials that can replicate composition functionality natural bone, promote quick full healing, reduce likelihood rejection infection desperately needed. Bone tissue engineering, combining biomaterial scaffolds pro-osteogenic drugs, provides support regeneration defects. development an effective scaffold for defect is urgent clinical need. present study investigates feasibility using microspheres based on α-tricalcium phosphate fibroin as osteoconductive matrix carrier controlled local delivery E7BMP-2 peptide, which E7 domain confers calcium chelation property, while BMP-2 mimicking peptide induces formation. We prepared phosphate/silk (α-TCP/SF) through high voltage electric field protocol α-TCP/SF cement slurry. This microspheres-based system was designed vehicles modified by to realize sustainable steady release peptide. In vitro cell tests experimental model cranial rats were used investigate benefits. results demonstrated peptide-bound functioned sustained enhanced osteogenic differentiation marrow mesenchymal stem cells rat calvarial Additionally, toxicity studies showed have good biocompatibility safety. Thus, these provide promising therapeutic strategy treatment

Language: Английский

Citations

0
Regulation of nanomaterials in peripheral nerve regeneration from a microenvironmental perspective: biological effects and mechanisms DOI Creative Commons

Xiner Tan,

Yiyuan Kang, Wei Du

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101808 - 101808

Published: April 29, 2025

Language: Английский

Citations

0
MicroRNA-21a-5p-modified macrophage exosomes as natural nanocarriers promote bone regeneration by targeting GATA2 DOI Creative Commons
Xin Luo,

Chun-Xiu Meng,

Yujue Zhang

et al.

Regenerative Biomaterials, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 1, 2023

Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities. In general, M2 macrophage facilitate promotion osteogenesis, as well, M1 play an important role early healing, confirmed by previous studies. However, it is not clear how involved response. MiR-21a-5p a highly expressed microRNA contrast to M2. Therefore, current work sought ascertain influence healing via exosomal miR-21a-5p and probable mechanism. We discovered that injecting exosomes overexpressing into defect locations enhanced regeneration vivo. Furthermore, directly targeting GATA2, accelerated MC3T3-E1 osteogenic differentiation. Our findings showed from may be transported osteoblasts target GATA2 enhance healing.

Language: Английский

Citations

8
Bioinspired zwitterionic lysine glycopolymers: Enhancing wound repair through microenvironment modulation for bacterial elimination and optimal immunoregulation DOI

Xingyou Wang,

Xiaoqing Sun, Jiahui Lei

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 57, P. 102354 - 102354

Published: June 14, 2024

Language: Английский

Citations

3
Research progresses on mitochondrial-targeted biomaterials for bone defect repair DOI Creative Commons

Shuze Wang,

Jialin Liu, Linxi Zhou

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

In recent years, the regulation of cell microenvironment has opened up new avenues for bone defect repair. Researchers have developed novel biomaterials to influence behavior osteoblasts and immune cells by regulating microenvironment, aiming achieve efficient Mitochondria, as crucial organelles involved in energy conversion, biosynthesis signal transduction, play a vital role maintaining integrity. Dysfunction mitochondria can detrimental effects on transformation differentiation stem cells, thereby hindering tissue regeneration. Consequently, targeted therapy strategies focusing emerged. This approach offers wide range applications reliable therapeutic effects, providing treatment option complex refractory diseases. studies, more been used restore mitochondrial function promote positive differentiation. The main directions are metabolism, biogenesis quality control. this review, we investigated mitochondria-targeted repair years from perspective progress strategies. We also summarized micro-molecular mechanisms affected them. Through discussions oxidative stress autophagy regulation, emphasized opportunities challenges faced biomaterials, clues developing generation materials.

Language: Английский

Citations

3
Bioceramic surface topography regulating immune osteogenesis DOI Creative Commons

Jianxin Hao,

Lin Du,

Yu He

et al.

BME Frontiers, Journal Year: 2024, Volume and Issue: 6

Published: Dec. 27, 2024

Objective: This study aims to clarify the effects of bioceramic interface cues on macrophages. Impact Statement: Recently, there have been many researches exploring topography macrophage polarization and cytokine secretion. However, underlying mechanisms macrophages still need exploring. provides insights into micro-groove surface structures Introduction: With development bone tissue engineering methods, bioceramics used for repair. After implantation bioceramics, innate immune response that occurs at materials can deeply influence subsequent inflammation regeneration progress. Therefore, exploration regulation will be beneficial promote effects. Methods: In this study, with are fabricated by digital light processing 3-dimensional printing technology. Then, different spacings (0, 25, 50, 75 μm) prepared separately explore Results: The large spacing structure M2 osteoinductive secretion macrophage. reason is induce directional arrangement so as change phenotype Further show osteogenic differentiation mesenchymal stem cells, which benefit osteogenesis osteointegration. Conclusion: offers an effective application potential method

Language: Английский

Citations

3
Effect of Er:YAG Pulsed Laser-Deposited Hydroxyapatite Film on Titanium Implants on M2 Macrophage Polarization In Vitro and Osteogenesis In Vivo DOI Open Access
Lin Ma, Min Li, Satoshi Komasa

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 349 - 349

Published: Dec. 26, 2023

In a previous study, we successfully coated hydroxyapatite (HAp) onto titanium (Ti) plates using the erbium-doped yttrium aluminum garnet pulsed-laser deposition (Er:YAG-PLD) method. this performed further experiments to validate in vitro osteogenic properties, macrophage polarization, and vivo osseointegration activity of HAp-coated Ti (HAp-Ti) screws. Briefly, HAp film surfaces screws via Er:YAG-PLD. The surface morphological, elemental, crystallographic analyses confirmed successful coating. polarization induction were evaluated macrophages rat bone marrow mesenchymal stem cells, properties studied. results showed that needle-shaped nano-HAp promoted early expression immunogenic genes induced excellent M2 properties. calcium osteocalcin production significantly higher HAp-Ti than uncoated Ti. implantation into femurs revealed materials had superior osteoinductive activities compared with Ti, as assessed by microcomputed tomography histology. Thus, on sandblasted Er:YAG-PLD enhances hard-tissue differentiation, new formation tissues surrounding implants both vivo.

Language: Английский

Citations

6
Efecto del precursor de calcio en las propiedades estructurales y microestructurales de la hidroxiapatita DOI Creative Commons
Claudia Fernanda Villaquirán Raigoza,

María Paula Durán Montoya,

Sonia Gaona Jurado

et al.

Revista EIA, Journal Year: 2024, Volume and Issue: 21(41)

Published: Jan. 1, 2024

Debido a que en la actualidad las afecciones óseas siguen siendo un desafío clínico significativo y soluciones son limitadas algunos casos poco efectivas, investigación alrededor de hidroxiapatita, principal componente mineral del hueso ha cobrado una importancia relevante. En este trabajo se analizó el efecto precursor calcio características estructurales microestructurales comparando los resultados obtenidos con hidroxiapatita extraída fuente natural. Mediante método reacción por combustión solución fueron sintetizados polvos utilizando como precursores carbonato extraído cáscara huevo nitrato comerciales. A su vez, natural fue bovino, sometió proceso lavado, fractura tratamiento térmico. Los grupos funcionales presentes muestras obtenidas determinados mediante espectroscopia infrarroja fases cristalinas difracción rayos-X. La microscopía electrónica transmisión permitió determinar morfología esférica partículas partir (de huevo) menor tamaño partícula (entre 20 50 nm); mientras que, comerciales presentaron no homogénea. mostraron seguido eficiente para obtención nanopartículas cuando obtiene temperatura 1100ºC. El proveniente obtener homogénea nanométrico.

Citations

1
The balance between helper T 17 and regulatory T cells in osteoimmunology and relevant research progress on bone tissue engineering DOI Creative Commons
Shuyu Zhu, Jing Zhou, Zhigang Xie

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(9)

Published: Sept. 1, 2024

Abstract Background Bone regeneration is a well‐regulated dynamic process, of which the prominent role immune system on bone homeostasis more and revealed by recent research. Before fully activation remodeling cells, needs to clean up microenvironment in facilitating repair initiation. Furthermore, this must be maintained properly various mechanisms over entire process. Objective This review aims summarize T‐helper 17/Regulatory T cell (Th17/Treg) balance discuss relevant progress tissue engineering. Results The response early stages crucial, especially impact Th17/Treg osteoclasts, mesenchymal stem cells (MSCs), osteoblasts activity. By virtue these knowledge advancements, innovative approaches engineering, such as nano‐structures, hydrogel, exosomes, are designed influence thereby augment regeneration. Conclusion Targeting promising strategy for developing new treatments enhance regeneration, thus offering potential breakthroughs injury clinics.

Language: Английский

Citations

1