Advances in tissue engineering of cancer microenvironment-from three-dimensional culture to three-dimensional printing DOI Creative Commons
Joana Marques, Patricia González‐Alva, Ruby Yu‐Tong Lin

et al.

SLAS TECHNOLOGY, Journal Year: 2023, Volume and Issue: 28(3), P. 152 - 164

Published: April 3, 2023

Cancer treatment development is a complex process, with tumor heterogeneity and inter-patient variations limiting the success of therapeutic intervention. Traditional two-dimensional cell culture has been used to study cancer metabolism, but it fails capture physiologically relevant cell-cell cell-environment interactions required mimic tumor-specific architecture. Over past three decades, research efforts in field 3D model fabrication using tissue engineering have addressed this unmet need. The self-organized scaffold-based shown potential microenvironment eventually bridge gap between 2D animal models. Recently, three-dimensional (3D) bioprinting emerged as an exciting novel biofabrication strategy aimed at developing compartmentalized hierarchical organization precise positioning biomolecules, including living cells. In review, we discuss advancements techniques for models, well their benefits limitations. We also highlight future directions associated technological advances, detailed applicative research, patient compliance, regulatory challenges achieve successful bed-to-bench transition.

Language: Английский

Parsing patterns: Emerging roles of tissue self-organization in health and disease DOI Creative Commons
Raúl Ramos, Benjamin Swedlund, Anand K. Ganesan

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(13), P. 3165 - 3186

Published: June 1, 2024

Patterned morphologies, such as segments, spirals, stripes, and spots, frequently emerge during embryogenesis through self-organized coordination between cells. Yet, complex patterns also in adults, suggesting that the capacity for spontaneous self-organization is a ubiquitous property of biological tissues. We review current knowledge on principles mechanisms patterning embryonic tissues explore how these apply to adult exhibit features patterning. discuss why pattern generation integral homeostasis healing tissues, illustrating it with examples from regenerative biology. examine aberrant underlies diverse pathological states, including inflammatory skin disorders tumors. Lastly, we posit based blueprints, targeted engineering pattern-driving molecular circuits can be leveraged synthetic biology organoids intricate patterns.

Language: Английский

Citations

7
Advancing cancer research through organoid technology DOI Creative Commons
Zeng Ge, Yifan Yu, Meiting Wang

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 8, 2024

The complexity of tumors and the challenges associated with treatment often stem from limitations existing models in accurately replicating authentic tumors. Recently, organoid technology has emerged as an innovative platform for tumor research. This bioengineering approach enables researchers to simulate, vitro, interactions between their microenvironment, thereby enhancing intricate interplay cells surroundings. Organoids also integrate multidimensional data, providing a novel paradigm understanding development progression while facilitating precision therapy. Furthermore, advancements imaging genetic editing techniques have significantly augmented potential organoids review explores application more precise simulations its specific contributions cancer research advancements. Additionally, we discuss evolving trends developing comprehensive utilizing technology.

Language: Английский

Citations

6
Integrated biosensors for monitoring microphysiological systems DOI
Lei Mou, Kalpana Mandal, Marvin Mecwan

et al.

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(20), P. 3801 - 3816

Published: Jan. 1, 2022

Microphysiological systems (MPSs), also known as organ-on-a-chip models, aim to recapitulate the functional components of human tissues or organs

Language: Английский

Citations

22
Lung Cancer Organoids: The Rough Path to Personalized Medicine DOI Open Access
Rachele Rossi, Maria Laura De Angelis,

Eljona Xhelili

et al.

Cancers, Journal Year: 2022, Volume and Issue: 14(15), P. 3703 - 3703

Published: July 29, 2022

Lung cancer is the leading cause of death worldwide. Despite significant advances in research and therapy, a dismal 5-year survival rate only 10–20% urges development reliable preclinical models effective therapeutic tools. characterized by high degree heterogeneity its histology, genomic landscape, response to therapies that has been traditionally difficult reproduce models. However, advent three-dimensional culture technologies opened new perspectives recapitulate vitro individualized tumor features anticipate treatment efficacy. The generation lung organoids (LCOs) encountered greater challenges as compared derived from other tumors. In last two years, many efforts have dedicated optimizing LCO-based platforms, resulting improved rates LCO production, purity, timing, long-term expansion. due complexity cancer, further are required order meet clinical needs. Here, we discuss evolution technology use LCOs basic translational research. Although field still infancy, prospective will likely lead strategies for drug testing biomarker identification, thus allowing more personalized approach patients.

Language: Английский

Citations

19
Oxygen-generating microparticles downregulate HIF-1α expression, increase cardiac contractility, and mitigate ischemic injury DOI Creative Commons
Kalpana Mandal,

Sivakoti Sangabathuni,

Reihaneh Haghniaz

et al.

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 159, P. 211 - 225

Published: Jan. 18, 2023

Language: Английский

Citations

11
3D tumor cultures for drug resistance and screening development in clinical applications DOI Creative Commons
Zheng Peng,

Xiaolan Lv,

Hao Sun

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 21, 2025

Tumor drug resistance presents a growing challenge in medical practice, particularly during anti-cancer therapies, where the emergence of drug-resistant cancer cells significantly complicates clinical treatment. In recent years, three-dimensional (3D) tumor culture technology, which more effectively simulates vivo physiological environment, has gained increasing attention research and applications. By mimicking cellular microenvironment, 3D technology not only recapitulates cell-cell interactions but also faithfully reproduces biological effects therapeutic agents. Consequently, is emerging as crucial tool biomedical research. We summarize benefits models organoid explore their application realm resistance, screening, personalized therapy, discuss potential prospects challenges transformation, with aim providing insights for optimizing treatment strategies advancing precision therapy.

Language: Английский

Citations

0
Bioprinted Patient‐Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine DOI Creative Commons

Jonghyeuk Han,

Hye‐Jin Jeong, Junyoung Choi

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Heterogeneity and the absence of a tumor microenvironment (TME) in traditional patient‐derived organoid (PDO) cultures limit their effectiveness for clinical use. Here, Embedded Bioprinting‐enabled Arrayed PDOs (Eba‐PDOs) featuring uniformly arrayed colorectal cancer (CRC) within recreated TME is presented. This model faithfully reproduces critical attributes, including elevated matrix stiffness (≈7.5 kPa) hypoxic conditions found CRC. Transcriptomic immunofluorescence microscopy analysis reveal that Eba‐PDOs more accurately represent actual tissues compared to PDOs. Furthermore, Eba‐PDO effectively capture variability CEACAM5 expression—a CRC marker—across different patients, correlating with patient classification differential responses 5‐fluorouracil treatment. method achieves an uniform size shape from same while preserving distinct morphological features among those individuals. These enable efficient development label‐free, morphology‐based predictive using supervised learning, enhancing its suitability applications. Thus, this approach PDO bioprinting promising tool generating personalized models advancing precision medicine.

Language: Английский

Citations

0
Breast cancer brain metastasis: evaluating the effectiveness of alginate-based organoids in metastasis modeling to replace matrigel DOI Creative Commons
Sepehr Zamani,

Fateme Sadat Bitaraf,

Mohammad Kamalabadi‐Farahani

et al.

BMC Research Notes, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 31, 2025

One of the most important and devastating side effects breast cancer is brain metastasis. Our understanding heterogeneity revolutionized by tumoral organoids seems promising for personalized medicine. This study aimed to generate a hydrogel-based metastasis organoid. Mouse metastatic tumor cells (4T1B) were isolated cultured from lesions mice with cancer. Different hydrogels, including alginate, carboxymethylcellulose, gelatin, collagen, matrigel, prepared. Pre-coated hydrogels in 96-well plates treated 4T1B cells. The morphology viability analyzed after 7 days. According our results, formed semi-regular cluster structures alginate hydrogel. In this group, cell survival rate formation three-dimensional significantly higher than other groups. For organoid cultures, there's lot research on natural synthetic scaffolds that are chemically or mechanically well-designed. present study, we used highly detected hydrogel best choice modeling.

Language: Английский

Citations

0
Microfabricated Organ-Specific Models of Tumor Microenvironments DOI

Jeong Min Oh,

Yongkuk Park, Jungwoo Lee

et al.

Annual Review of Biomedical Engineering, Journal Year: 2025, Volume and Issue: 27(1), P. 307 - 333

Published: May 1, 2025

Despite the advances in detection, diagnosis, and treatments, cancer remains a lethal disease, claiming lives of more than 600,000 people United States alone 2024. To accelerate development new therapeutic strategies with improved responses, significant efforts have been made to develop microfabricated vitro models tumor microenvironments (TMEs) that address limitations animal-based models. These incorporate several advanced tissue engineering techniques better reflect organ- patient-specific TMEs. Additionally, integrated next-generation single-cell omics technologies provide unprecedented insights into patient's cellular molecular heterogeneity complexity. This review provides an overview recent understanding outlines key TME elements can be captured enhance their physiological relevance. We highlight unique characteristics organs origin or sites dissemination.

Language: Английский

Citations

0
The Patient-Derived Cancer Organoids: Promises and Challenges as Platforms for Cancer Discovery DOI Open Access

JuneSung Bae,

Yun Sik Choi,

Gun-Sik Cho

et al.

Cancers, Journal Year: 2022, Volume and Issue: 14(9), P. 2144 - 2144

Published: April 25, 2022

The cancer burden is rapidly increasing in most countries, and thus, new anticancer drugs for effective therapy must be developed. Cancer model systems that recapitulate the biological processes of human cancers are one cores drug development process. PDCO has emerged as a unique preserves genetic, physiological, histologic characteristics original cancer, including inter- intratumoral heterogeneities. Due to these advantages, PCDO increasingly investigated screening efficacy testing, preclinical patient stratification, precision medicine selecting patients. Here, we review prospects limitations compared conventional models. With advances culture success rates, co-culture with tumor microenvironment, organoid-on-a-chip technology, automation will become promising develop medicine.

Language: Английский

Citations

15