Advances in tissue engineering of cancer microenvironment-from three-dimensional culture to three-dimensional printing DOI Creative Commons
Joana Marques, Patricia González‐Alva, Ruby Yu‐Tong Lin

et al.

SLAS TECHNOLOGY, Journal Year: 2023, Volume and Issue: 28(3), P. 152 - 164

Published: April 3, 2023

Cancer treatment development is a complex process, with tumor heterogeneity and inter-patient variations limiting the success of therapeutic intervention. Traditional two-dimensional cell culture has been used to study cancer metabolism, but it fails capture physiologically relevant cell-cell cell-environment interactions required mimic tumor-specific architecture. Over past three decades, research efforts in field 3D model fabrication using tissue engineering have addressed this unmet need. The self-organized scaffold-based shown potential microenvironment eventually bridge gap between 2D animal models. Recently, three-dimensional (3D) bioprinting emerged as an exciting novel biofabrication strategy aimed at developing compartmentalized hierarchical organization precise positioning biomolecules, including living cells. In review, we discuss advancements techniques for models, well their benefits limitations. We also highlight future directions associated technological advances, detailed applicative research, patient compliance, regulatory challenges achieve successful bed-to-bench transition.

Language: Английский

Mimicking Tumor Metastasis Using a Transwell‐Integrated Organoids‐On‐a‐Chip Platform DOI

Maike Chen,

Shan Han, Qian Tao

et al.

Small, Journal Year: 2024, Volume and Issue: 20(27)

Published: Feb. 2, 2024

Abstract The mortality rate among cancer patients is primarily attributed to tumor metastasis. evaluation of metastasis potential provides a powerful framework for personalized therapies. However, little work has so far been undertaken precisely model in vitro, hindering the development preventive and therapeutic interventions. In this work, tumor‐metastasis‐mimicked Transwell‐integrated organoids‐on‐a‐chip platform (TOP) evaluating metastatic developed. Unlike conventional Transwell device detecting cell migration, engineered facilitates assessment patient‐derived organoids (PDO). Furthermore, novel chamber with hexagon‐shaped structure developed mimic migration cells into surrounding tissues, allowing horizontal direction. As proof‐of‐concept demonstration, clusters are further evaluated at protein, genetic, phenotypic levels. addition, preliminary drug screening highlight using combat cancers. summary, TOP offers unique capabilities contributes

Language: Английский

Citations

2
Dot extrusion bioprinting of spatially controlled heterogenous tumor models DOI Creative Commons
Xiaoyun Wei,

Beisi Huang,

Keke Chen

et al.

Materials & Design, Journal Year: 2022, Volume and Issue: 223, P. 111152 - 111152

Published: Sept. 13, 2022

Bioprinting of cell-laden hydrogel constructs providing three-dimensional (3D) spatial pattern capacity and suitable cellular microenvironment have become essential tools in the field tissue engineering. For heterogeneous development, printing approaches permitting controllable deposition multicellular components a flexible manner are urgently needed. Here, facile dot-extrusion (DEP) system for one-step generation gelatin methacrylate (GelMa) beads (GHBs) is developed, which allows size-tunable GHBs programmable positioned into complex 3D constructs. The GelMa situ semi-gelled at printhead, thus enabling onto platform, as result improved simplicity, fidelity flexibility. size position adjustable by programming G-code parameters. Further, integrating multiple printheads, encapsulating different can be printed to fabricate constructs, maintain post-printed cell viability over 95%. As an example application, two tumor-stroma phases were developed through regionally tumor cells normal fibroblasts juxtapositional or overlapping microcapsule structures, giving access study interactions microenvironments. DEP holds promise creation tissues toward various biology studies.

Language: Английский

Citations

10
3D printed porous microgel for lung cancer cells culture in vitro DOI Creative Commons
Qipeng Hu, Xuan Liu,

Haofan Liu

et al.

Materials & Design, Journal Year: 2021, Volume and Issue: 210, P. 110079 - 110079

Published: Aug. 30, 2021

Currently, in vitro cancer cell culture technology is very important for research. Existing studies have shown that two-dimensional (2D) environment may cause significant differences from tumor cells vivo, resulting high failure rates when the therapies developed 2D models translated to clinic. And compared with culture, isotropic three-dimensional (3D) can construct an more similar body. Here, we utilized 3D printed porous microgel lung and also explored possible mechanism of how regulates actin cytoskeleton. Compared cultured cells, are those extracted existing gold standard: mouse transplanted tumors, terms ROCK-actin pathway. In addition, this study revealed ROCK pathway altered by played role regulating drug sensitivity cells. Hence, a promising method, which shows potentional application future research development.

Language: Английский

Citations

13
The use of organoids in food research DOI
Janelle E. Stanton, Andreas M. Grabrucker

Current Opinion in Food Science, Journal Year: 2022, Volume and Issue: 49, P. 100977 - 100977

Published: Dec. 9, 2022

Language: Английский

Citations

8
Advances in tissue engineering of cancer microenvironment-from three-dimensional culture to three-dimensional printing DOI Creative Commons
Joana Marques, Patricia González‐Alva, Ruby Yu‐Tong Lin

et al.

SLAS TECHNOLOGY, Journal Year: 2023, Volume and Issue: 28(3), P. 152 - 164

Published: April 3, 2023

Cancer treatment development is a complex process, with tumor heterogeneity and inter-patient variations limiting the success of therapeutic intervention. Traditional two-dimensional cell culture has been used to study cancer metabolism, but it fails capture physiologically relevant cell-cell cell-environment interactions required mimic tumor-specific architecture. Over past three decades, research efforts in field 3D model fabrication using tissue engineering have addressed this unmet need. The self-organized scaffold-based shown potential microenvironment eventually bridge gap between 2D animal models. Recently, three-dimensional (3D) bioprinting emerged as an exciting novel biofabrication strategy aimed at developing compartmentalized hierarchical organization precise positioning biomolecules, including living cells. In review, we discuss advancements techniques for models, well their benefits limitations. We also highlight future directions associated technological advances, detailed applicative research, patient compliance, regulatory challenges achieve successful bed-to-bench transition.

Language: Английский

Citations

4