Comparison of Anticancer Activities and Biosafety Between Salmonella enterica Serovar Typhimurium ΔppGpp and VNP20009 in a Murine Cancer Model DOI Creative Commons
Xiaoqing Liu, Yanxia Guo, Yujie Sun

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 29, 2022

Salmonella Typhimurium defective in guanosine 5′-diphosphate-3′-diphosphate (ppGpp) synthesis (ΔppGpp) is an attenuated strain with good biosafety and excellent anticancer efficacy. It has been widely applied preclinical studies of therapy for various types solid cancer. VNP20009 another genetically modified auxotrophic 108-kb deletion, purI − , msbB many single nucleotide polymorphisms (SNPs); it shown promising therapeutic efficacy tumor models entered phase I clinical trials. Here, the invasion activities virulence ΔppGpp were obviously lower than those when tested cancer cells vitro . In addition, MC38 tumor-bearing mice showed comparable suppression treated or intravenously. However, ΔppGpp-treated 16.7% complete eradication prolonged survival mice, whereas higher toxicity to animals, even equal size individually. Moreover, we found decreased levels inflammatory cytokines circulation but strengthened immune boost microenvironments mice. Therefore, engineered high potential therapeutics, a option future therapy.

Language: Английский

Promising dawn in tumor microenvironment therapy: engineering oral bacteria DOI Creative Commons
Zifei Wang, Wansu Sun, Ruixue Hua

et al.

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: March 13, 2024

Abstract Despite decades of research, cancer continues to be a major global health concern. The human mouth appears multiplicity local environments communicating with other organs and causing diseases via microbes. Nowadays, the role oral microbes in development progression has received increasing scrutiny. At same time, bioengineering technology nanotechnology is growing rapidly, which physiological activities natural bacteria are modified improve therapeutic efficiency cancers. These engineered were transformed achieve directed genetic reprogramming, selective functional reorganization precise control. In contrast endotoxins produced by typical genetically bacteria, flora exhibits favorable biosafety characteristics. To outline current cognitions upon microbes, cancers, related literatures searched reviewed based on PubMed database. We focused number mechanisms associated tumor microenvironment, involve occurrence development. Whether engineering can possible application therapy worth consideration. A deeper understanding relationship between may enhance our knowledge pathogenesis thus providing new insights strategies for prevention treatment.

Language: Английский

Citations

69
Liposomes as Immunotherapeutic Carriers: A Game-Changer in Cancer Therapy DOI

Sepehr Hoseinzadeh Moghaddam,

Abulfazl Vatankhah,

Fatemeh Oroojalian

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106847 - 106847

Published: March 1, 2025

Language: Английский

Citations

3
Salmonella-mediated methionine deprivation drives immune activation and enhances immune checkpoint blockade therapy in melanoma DOI Creative Commons
Sujin Zhou, Shiwei Zhang, Kexin Zheng

et al.

Journal for ImmunoTherapy of Cancer, Journal Year: 2024, Volume and Issue: 12(2), P. e008238 - e008238

Published: Feb. 1, 2024

Background Although immune checkpoint inhibitor (ICI)-based therapy is advantageous for patients with advanced melanoma, resistance and relapse are frequent. Thus, it crucial to identify effective drug combinations develop new therapies the treatment of melanoma. SGN1, a genetically modified Salmonella typhimurium species that causes targeted deprivation methionine in tumor tissues, currently under investigation clinical trials. However, inhibitory effect SGN1 on melanoma benefits combination ICIs remain largely unexplored. Therefore, this study aims investigate antitumor potential its ability enhance efficacy antibody-based programmed cell death-ligand 1 (PD-L1) inhibitors murine Methods The activity PD-L1 was studied through models. Further, Cancer Genome Atlas-melanoma cohort clustered using ConsensusClusterPlus based deprivation-related genes, characterization performed xCell, Microenvironment Cell Populations-counter, Estimation Stromal Immune cells MAlignant Tumor tissues Expression data, immunophenoscore (IPS) analyses. messenger RNA data death-1 (PD-1) immunotherapy response were obtained from Gene Omnibus database. Set Enrichment Analysis deprivation-up gene set determine differences between pretreatment responders non-responders. Results This showed both, intratumoral intravenous administration subcutaneous B16-F10 melanomas, suppress growth, which associated an activated CD8+T-cell microenvironment. Combination systemic anti-PD-L1 resulted better than individual monotherapies, respectively, high therapeutic increase level tumor-specific CD8 + T cells. Two clusters consisting genes identified. Patients cluster 2 had higher expression methionine_deprivation_up outcomes, infiltration levels compared 1. Western blot, IPS analysis, revealed deficiency may show ICI Conclusions： reports -based as potent anticancer agent against lays groundwork synergistic brought about by improving microenvironment melanomas.

Language: Английский

Citations

11
Genetically Engineered Bacteria as a Promising Therapeutic Strategy Against Cancer: A Comprehensive Review DOI Open Access

Zahra Zahedifard,

Shirin Mahmoodi, Abdolmajid Ghasemian

et al.

Biotechnology and Applied Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

ABSTRACT As a significant cause of global mortality, the cancer has also economic impacts. In era therapy, mitigating side effects and costs overcoming drug resistance is crucial. Microbial species can grow inside tumor microenvironment inhibit growth through direct killing cells immunoregulatory effects. Although microbiota or their products have demonstrated anticancer effects, possibility acting as pathogens exerting in certain individuals risk. Hence, several genetically modified/engineered bacteria (GEB) been developed to this aim with ability diagnosing selective targeting destruction cancers. Additionally, GEB are expected be considerably more efficient, safer, permeable, less costly, invasive theranostic approaches compared wild types. Potential strains such Escherichia coli (Nissle 1917, MG1655), Salmonella typhimurium YB1 SL7207 ( aroA gene deletion), VNP20009 (∆msbB/∆purI) ΔppGpp (P Tet P BAD ), Listeria monocytogenes Lm at ‐LLO combat cells. When used tandem conventional treatments, substantially improve efficacy therapy outcomes. addition, public acceptance, optimal timing (s), duration dose identification, interactions other host cells, efficacy, safety quality, potential risks ethical dilemmas include major challenges.

Language: Английский

Citations

2
The role of bacteria and its derived biomaterials in cancer radiotherapy DOI Creative Commons
Yu Zhang,

Ruizhe Huang,

Yunchun Jiang

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2022, Volume and Issue: 13(10), P. 4149 - 4171

Published: Oct. 20, 2022

Bacteria-mediated anti-tumor therapy has received widespread attention due to its natural tumor-targeting ability and specific immune-activation characteristics. It made significant progress in breaking the limitations of monotherapy effectively eradicating tumors, especially when combined with traditional therapy, such as radiotherapy. According their different biological characteristics, bacteria derivatives can not only improve sensitivity tumor radiotherapy but also protect normal tissues. Moreover, genetically engineered bacteria-based biomaterials have further expanded scope applications In this review, we summarized relevant researches on application recent years, expounding that bacteria, bacterial directly enhance effect by improving microenvironment (TME) immune effects. Furthermore, some probiotics tissues organs intestines from radiation via anti-inflammatory, anti-oxidation apoptosis inhibition. conclusion, prospect will be very extensive, safety mechanism need evaluated studied.

Language: Английский

Citations

34
The potential use of bacteria and bacterial derivatives as drug delivery systems for viral infection DOI Creative Commons
Amirhosein Faghihkhorasani,

Hanan Hassan Ahmed,

Noor Muhammad Mashool

et al.

Virology Journal, Journal Year: 2023, Volume and Issue: 20(1)

Published: Oct. 3, 2023

Abstract Viral infections in humans are responsible for fatalities worldwide and contribute to the incidence of various human ailments. Controllable targeted medicine delivery against many illnesses, including viral infection, may be significantly aided by using bacteria bacteria-derived products. They accumulate diseased tissues despite physical obstacles, where they can launch antiviral immunity. The ability genetically chemically modify them means that vaccinations manufactured delivered affected more safely effectively. objective this study is provide an overview latest advancements field utilizing bacterial derivatives as carriers administering medication treat diseases such SARS-CoV-2, hepatitis B virus, C immunodeficiency papillomavirus, influenza, Ebola virus. Graphical

Language: Английский

Citations

20
A Review of the Use of Native and Engineered Probiotics for Colorectal Cancer Therapy DOI Open Access
Huawen Han, Yifan Zhang,

Haibo Tang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3896 - 3896

Published: March 31, 2024

Colorectal cancer (CRC) is a serious global health concern, and researchers have been investigating different strategies to prevent, treat, or support conventional therapies for CRC. This review article comprehensively covers CRC therapy involving wild-type bacteria, including probiotics oncolytic bacteria as well genetically modified bacteria. Given the close relationship between gut microbiota, it crucial compile present comprehensive overview of bacterial used in context colorectal cancer. It evident that use native engineered necessitates research focused on enhancing therapeutic properties probiotic strains.. Genetically might be designed produce particular molecules target cells more effectively cure patients.

Language: Английский

Citations

9
Recent advances in bacteria-mediated cancer therapy DOI Creative Commons

Shuya Liang,

Chao Wang,

Yingchun Shao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 12, 2022

Cancer is among the leading cause of deaths worldwide. Although conventional therapies have been applied in fight against cancer, poor oxygen, low extracellular pH, and high interstitial fluid pressure tumor microenvironment mean that these treatments fail to completely eradicate cancer cells. Recently, bacteria increasingly considered be a promising platform for therapy thanks their many unique properties, such as specific tumor-targeting ability, motility, immunogenicity, use gene or drug carriers. Several types already used solid metastatic therapies, with results. With development synthetic biology, engineered endowed controllable expression therapeutic proteins. Meanwhile, nanomaterials widely modify targeted delivery, photothermal therapy, magnetothermal photodynamic while promoting antitumor efficiency synergistic therapies. This review will provide brief introduction foundation bacterial biotherapy. We begin by summarizing recent advances different multiple then discuss future prospects bacteria-mediated

Language: Английский

Citations

29
Bacteria and Bacterial Components as Natural Bio-Nanocarriers for Drug and Gene Delivery Systems in Cancer Therapy DOI Creative Commons
Rui Zong,

Hainan Ruan,

Chan‐Min Liu

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2490 - 2490

Published: Oct. 19, 2023

Bacteria and bacterial components possess multifunctional properties, making them attractive natural bio-nanocarriers for cancer diagnosis targeted treatment. The inherent tropic motile nature of bacteria allows to grow colonize in hypoxic tumor microenvironments more readily than conventional therapeutic agents other nanomedicines. However, concerns over biosafety, limited antitumor efficiency, unclear tumor-targeting mechanisms have restricted the clinical translation application based on components. Fortunately, therapies combined with engineering strategies nanotechnology may be able reverse a number challenges bacterial/bacterial component-based biotherapies. Meanwhile, tend enhance versatility bionanoplasmic nanoplatforms improve biosafety inhibit tumorigenesis metastasis. This review summarizes advantages therapy, outlines combinatorial nanocarriers components, discusses their applications.

Language: Английский

Citations

16
Personalized Bacteria Loaded with Autoantigens for the Enhancement of Tumor Immunotherapy DOI

Chenghu Wu,

Wenjuan He, Yue Chen

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(11)

Published: Jan. 5, 2023

Abstract Currently, tumor immunotherapy is becoming a new revolution in treatment. Generated from cell lysate (TCL) or irradiated cells, the whole antigen‐based vaccines have possibility to enhance antitumor immune response without survival surveillance personalized immunotherapy. Here, polydopamine nanoparticles (PDA NPs) after self‐polymerization are covalently coated with TCL form PDA@CL. Engineered Salmonella (EnS) wrapped PDA@CL (EnS@PDA@CL) targeted localization of site by intravenous administration. EnS@PDA@CL delivered autologous antigen‐containing hypoxia regions through blood circulation. The particles promote maturation dendritic cells (DCs), thus eliciting infiltration antigens specific cytotoxic T lymphocytes, significantly triggering immunity. regression Panc02 mice confirms therapeutic potential clinical

Language: Английский

Citations

13