Gastroenterology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 1, 2024
Language: Английский
Journal of Hepatology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
4
Gastroenterology, Journal Year: 2024, Volume and Issue: unknown
Published: April 1, 2024
Language: Английский
Citations
15
Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 118, P. 105557 - 105557
Published: Jan. 19, 2024
Human infections with Rocahepevirus ratti genotype C1 (HEV-C1) in Hong Kong of China, Canada, Spain, and France have drawn worldwide concern towards Rocahepevirus. This study conducted a global genetic analysis Rocahepevirus, aiming to furnish comprehensive molecular insights promote further research. We retrieved 817 sequences from the GenBank database through October 31, 2023, categorizing them according research, sample collection area date, genotype, host, sequence length. Subsequently, we descriptive epidemiological, phylogenetic evolutionary, protein polymorphism (in length identity) analyses on these sequences. genomes were identified across twenty-eight countries, predominantly Asia (71.73%, 586/817) Europe (26.44%, 216/817). The HEV-C1 dominates (77.2%, 631/817), while newly discovered genotypes (C3/C4/C5 other unclassified genotypes) primarily (25/120) China (91/120). Muridae animals (72.5%, 592/817) serve as primary hosts for encompassing species families Soricidae, Hominidae, Mustelidae, Cricetidae. Additionally, (C1 genotype) sewage samples recently. evolution exhibits considerable variation. Specifically, can be classified into at least six groups (G1 G6), human distributed multiple evolutionary clades. overall ORF1 ORF2 amino acid lengths significantly different (P < 0.001) genotypes. HEV-C1/C2/C3 HEV-C4/C5 displayed substantial differences identity (58.4%-59.6%). identification has expanded numerous particularly European Asian coinciding an expanding host range emergence new path is intricate, where globally internally forms exhibiting diverse variation within HEV-C1. Significant exist Given Rocahepevirus's shift animal virus zoonotic pathogen, cooperation monitoring vital.
Language: Английский
Citations
7
Virus Research, Journal Year: 2024, Volume and Issue: 344, P. 199364 - 199364
Published: March 28, 2024
The hepatitis E virus (HEV) can cause acute and chronic in humans. Whereas HEV genotypes 1–4 of species Paslahepevirus balayani are commonly found humans, infections with ratHEV (species Rocahepevirus ratti) were previously considered to be restricted rats. However, several cases human have been described recently. To investigate the zoonotic potential this virus, a genomic clone was constructed here based on sequence data strain pt2, originally identified patient from Hongkong. For comparison, clones R63 rat genotype 3 47832mc used. After transfection vitro-transcribed RNA into hepatoma cell line HuH-7-Lunet BLR, replication shown for all strains by increasing genome copy numbers culture supernatants. These cells developed persistent infections, particles supernatant as well viral antigen within demonstrated. All three generated successfully infected fresh BLR cells. In contrast, lines HuH-7 PLC/PRF/5 could only lesser extent R63. Infection rat-derived 9, MH1C1 H-4-II-E did not result efficient either strain. results indicate that rats humans infect efficiency is strongly dependent investigated other should tested future identify permissive represent useful tool research investigating pathogenicity ratHEV.
Language: Английский
Citations
2
Pathogens, Journal Year: 2024, Volume and Issue: 13(8), P. 633 - 633
Published: July 29, 2024
Hepatitis E virus belonging to the Rocahepevirus ratti species, genotype HEV-C1, has been extensively reported in rats Europe, Asia and North America. Recently, human cases of hepatitis associated with HEV-C1 infection have reported, but zoonotic nature rat-HEV remains controversial. The transmission route is unidentified requires further investigation. HEV strains Paslahepevirus balayani same Hepeviridae family, including HEV-3 usually found pigs, also sporadically identified rats. We sampled 115 (liver, lung, feces) between 2020 2023 Northeast Italy detection was carried out by using Reverse Transcription PCR. RNA detected 3/115 (2.6%) who tested positive for paired intestinal contents liver samples. Overall, none strains. In conclusion, our results confirm presence HEV-rat a prevalence similar previous studies show that there wide heterogeneity circulation. species some acute suggests may be an underestimated source infections. This finding, geographically widespread rats, raises questions about role as hosts both possibility transmission.
Language: Английский
Citations
2
Hepatology Communications, Journal Year: 2024, Volume and Issue: 8(11)
Published: Oct. 17, 2024
Background: HEV is a positive-sense, single-stranded RNA virus of the Hepeviridae family. Although accounts for more than 3 million symptomatic cases viral hepatitis per year, specific anti-HEV therapy and knowledge about pathogenesis are scarce. Methods: To gain deeper understanding infectious cycle guide development novel antiviral strategies, we here used an RNAi mini screen targeting selection kinases, including mitogen-activated protein receptor tyrosine Src-family kinases. Further, state-of-the-art infection models, primary human hepatocytes athymic nude rats. Results: Upon knockdown kinase Yes1, significant reduction susceptibility could be observed, suggesting important role Yes1 in cycle. Selective inhibition activity resulted hepatoma cells hepatocytes, as well rat vivo model system. Subsequent analysis Y1KI during life indicated early onset infection. Conclusions: We identified dependence on signaling, which may contribute to so far scarce HEV’s future. Moreover, provide drug candidate specifically host factor.
Language: Английский
Citations
2
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(51)
Published: Dec. 9, 2024
Hepatitis E virus (HEV; family
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 28, 2024
Abstract Hepatitis E virus (HEV) is an emerging pathogen responsible for acute viral hepatitis globally. There no specific antiviral and non-universal vaccine, partly due to limitations in its vitro cultivation. Although HEV mainly fecal-oral transmitted excreted the feces high titers, role of gut HEV-induced disease remains unexplored. Little known about how spreads from liver, or potentially acting as reservoir. To address this, we developed infection model using human intestinal enteroids (HIEs). was done using: a) differentiated 3D-HIEs; b) 2D-HIEs transwells; c) electroporation HIEs with capped RNA. 3D HEV-3 strains resulted a limited increase Infection yielded similar replication levels, shedding apical side epithelial layer. Importantly, RNA sustained load over time supernatant, detection infectious particles. Likewise, HEV-1 3 luciferase replicon RNA, 5-fold 20-fold signal observed. Treatment current off-label ribavirin effectively inhibited subgenomic full-length genomic electroporated HIEs. Moreover, here investigate cellular tropism Abundant ORF2 antigen detected infected HIEs, including proliferating cells, enterocytes enteroendocrine increased mRNA expression latter cell type. Overall, established discovered that fast turnover intestine important feature enables efficient replication. Furthermore, promotes differentiation towards which can infect.
Language: Английский
Citations
0
European Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: 3(2), P. 1 - 5
Published: March 7, 2024
Background: Sudan, as a developing country, suffers from most of the risk factors related to transmission hepatitis E virus (HEV). Objective: This study aimed determine sero-frequency HEV in cancer patients and healthcare workers its associated factors. Methods: A descriptive cross-sectional design was followed, recruited populations were 120 workers. Sociodemographic data subject collected using questionnaire patient records. HEV-specific anti-HEV IgM IgG antibodies screened by competitive ELISA technique. Chi-square test determined for association between sero-positivity andsubject information. Results: The percentage positive 50% (60/120) 6.7% (8/120) while group 25.8% (31/120) 4.2% (5/120) respectively. documented among consumption raw meat 94.2% (113/120), contact with animals 88.3% (106/120), history blood transfusion 81.7% (98/120) commonly found (113/120) group. significant observed sero-positive (p-value = 0.005). Conclusion: at high rate both groups, which requires further studies on prevalence, epidemiology, prevention.
Language: Английский
Citations
0
Gastroenterology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 1, 2024
Language: Английский
Citations
0