Effects of bicarbonate on antioxidant, immune, osmolytic and metabolic capabilities of Scylla paramamosain

Aquaculture, Journal Year: 2024, Volume and Issue: 594, P. 741409 - 741409

Published: Aug. 2, 2024

Language: Английский

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Changes in Physiological Homeostasis in the Gills of Litopenaeus vannamei Under Carbonate Alkalinity Stress and Recovery Conditions

Fishes, Journal Year: 2024, Volume and Issue: 9(11), P. 463 - 463

Published: Nov. 15, 2024

Carbonate alkalinity (CA) is the major toxic factor that interferes with survival and growth of shrimp in saline–alkaline water. Gills are main entry organ for CA toxicity shrimp. In this study, low-salinity cultured Litopenaeus vannamei were exposed to 5 mmol/L stress 7 days then recovered explore physiological changes gills under recovery conditions at multiple biological levels. The results showed increased activities antioxidative biochemical indexes (T-AOC, T-SOD, POD) relative expression levels romo1, nrf2, gpx genes, while it decreased sod hsp70 genes. addition, also genes involved endoplasmic reticulum (ER) (bip, ire1, xbp1), immunity (alf, crus, pen-3, lys, propo), apoptosis (casp-3), detoxification metabolism (cyp450 gst), osmotic adjustment (ca, nka-α, nka-β, vatp, nhe, clc, aqp, tip4, ccp). Although some reversed after was relieved, they still could not effectively recover control level. These reveal has a negative impact on homeostasis by inducing oxidation ER interfering immunity, apoptosis, detoxification, adjustment.

Language: Английский

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Study on the physiological responses and tolerance mechanisms to subchronic carbonate alkalinity exposure in the gills of Paramisgurnus dabryanus

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117319 - 117319

Published: Nov. 1, 2024

Given the reduction of freshwater resources, saline-alkaline aquaculture has emerged as an effective approach to expand fishery's accessible space. High carbonate alkalinity (CA) is a major stressor for aquatic organisms in environments. Paramisgurnus dabryanus potential species culture water, making it ideal model investigating physiological responses and tolerance mechanisms CA exposure fishes. In current study, P. were exposed 15 30 mmol/L NaHCO

Language: Английский

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Physiological Adaptation of Fenneropenaeus chinensis in Response to Saline–Alkaline Stress Revealed by a Combined Proteomics and Metabolomics Method

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 488 - 488

Published: June 30, 2024

The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize abundant inland saline-alkaline water, we studied physiological effects high carbonate alkalinity stress and pH on

Language: Английский

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LC-MS untargeted metabolomics reveals metabolic disturbance and ferroptosis in MWCNTs-induced hepatotoxicity of Cyprinus carpio

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 275, P. 107078 - 107078

Published: Sept. 2, 2024

Language: Английский

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Effects of Tannin Supplementation in Diet on the Resistance to Ammonia Stress of Pacific White Shrimp Litopenaeus vannamei

Aquaculture Nutrition, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 16

Published: May 13, 2024

Tannin (TA), as a natural phenolic compound with strong antioxidant activity, has been used feed additive for various animals. In this study, we fed diet containing 800 mg/kg of tannin on

Language: Английский

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Integrated Analysis of Transcriptome and Metabolome Reveals Chronic Low Salinity Stress Responses in the Muscle of Exopalaemon Carinicauda

Published: Jan. 1, 2024

Language: Английский

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Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(7), P. e1012321 - e1012321

Published: July 11, 2024

Vibriosis is one of the most serious diseases that commonly occurs in aquatic animals, thus, shaping a steady inherited resistance trait organisms has received highest priority aquaculture. Whereas, mechanisms underlying development such are mostly elusive. In this study, we constructed vibriosis-resistant and susceptible families Pacific white shrimp Litopenaeus vannamei after four generations artificial selection. Microbiome sequencing indicated can successfully develop colonization against Vibrio infections. This was characterized by microbial community structure with specific enrichment single probiotic species (namely Shewanella algae ), notably, its formation inheritable might be memorized host epigenetic remodeling. Regardless infection status, group genes specifically activated resistant family through disruption complete methylation. Specifically, hypo-methylation hyper-expression related to lactate dehydrogenase (LDH) iron homeostasis provide rich sources carbon (lactate) ions for S . , which directly results reduction load shrimp. Lactate feeding increased survival shrimp, while knockdown LDH gene decreased when infected pathogens. addition, treatment methyltransferase inhibitor 5-azacytidine resulted upregulations some protein processing genes, significant simultaneous Our suggest as information host, played pivotal role vibriosis resistance. The findings study will aid disease control selection superior lines high

Language: Английский

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Effects of Carbonate Alkalinity on Antioxidants, Immunity and Intestinal Flora of Penaeus vannamei

Fishes, Journal Year: 2024, Volume and Issue: 10(1), P. 12 - 12

Published: Dec. 30, 2024

The purpose of this study was to investigate the physiological and biochemical changes hepatopancreas intestinal microbial structure Penaeus vannamei under various levels carbonate alkalinity stress. After (body length 14.24 ± 2.13 cm, body weight 26.31 3.26 g) subjected 96 h stress, stress were E8 (8 mmol/L), E18 (18 mmol/L) E28 (28 respectively. activity antioxidant enzymes determined by enzyme markers, then microorganisms analyzed high-throughput sequencing technology. results showed that, high alkalinity, mortality rate as 75%, cells obvious deformation, abnormal nuclear shapes, serious cell vacuolation. Under superoxide dismutase activity, catalase glutathione peroxidase in significantly lower than those control group (p < 0.05), malondialdehyde content that 0.05). Alkaline phosphatase experimental different from Moreover, 16SrDNA abundance Proteobacteria decreased 0.05) Bacteroides increased At genus level, Chrysobacteria functional prediction COG KEGG RNA polymerase sigma-70 factor is direct bacterial or plastid core specific promoter elements are situated 10 35 base-pairs upstream transcription-initiation points—in treatment group, higher group. signal transduction histidine kinase not only indicated would change increase culture environment, but they also This could provide theoretical reference for saline–alkali land culture.

Language: Английский

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Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Water, Journal Year: 2023, Volume and Issue: 15(19), P. 3519 - 3519

Published: Oct. 9, 2023

In agricultural and fishery production, the occurrence of cyanobacterial blooms contamination freshwater systems with microplastics have become increasingly important research focuses. However, individual combined toxic mechanisms these two pollutants are not yet fully understood. Therefore, in this study, we analyzed effects stressors, microcystins (MC) nanoplastics (NP), on transcriptome metabolome hepatopancreas river clams. RNA metabolites extracted from clams treated MC, NP, a combination MC NP were used to construct standardized cDNA libraries, which then subjected integrated analysis. Significant enrichment 49 pathways, 34 44 pathways was observed group compared control group, NP-MC respectively. three experimental groups, found that lysosome pathway, affects immune function cell apoptosis, enriched numerous differentially expressed genes metabolites. Changes ATP6N ADP may impair lysosomal acidification disrupt normal degradation processes, indicating interference hepatopancreatic metabolism pollutants. Interestingly, significant alterations cathepsin family, downregulation genes, along ATP6N, implies potential disruption proteolysis. purine expression levels suggests an impact system by NP-MC. conclusion, while there is some overlap damage caused MCs, MPs, NP-MC, further necessary understand their effects.

Language: Английский

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