Enhancing diabetic muscle repair through W-GA nanodots: a nanomedicinal approach to ameliorate myopathy in type 2 diabetes

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Abstract Objective Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder that significantly impairs muscle regeneration following injuries, contributing to numerous complications and reduced quality of life. There an urgent need for therapeutic strategies can enhance alleviate these pathological mechanisms. In this study, we evaluate the efficacy W-GA nanodots, which are composed gallic acid (GA) tungstate (W6+), on in type (T2D)-induced injury, with focus their anti-inflammatory antioxidative effects. Methods This study synthesized ultrasmall nanodots were optimized improved stability bioactivity under physiological conditions. vitro assessments included cell viability, apoptosis, reactive oxygen species (ROS) generation, myotube differentiation C2C12 myoblasts hyperglycemic vivo, T2D was induced C57BL/6 mice, followed by injury treatment W-GA. Muscle repair, fibrosis, functional recovery assessed through histological analysis gait using CatWalk system. Results The enhanced proliferation, decreased ROS, apoptosis vitro. compared control group, W-GA-treated group exhibited notably regeneration, recovery. modulated inflammatory response oxidative stress diabetic tissues, facilitating regenerative dynamics function. Conclusions effectively counter mechanisms myopathy enhancing capacity reducing inflammation. nanomedicine approach offers promising avenue improving health overall life individuals suffering from T2D. However, further studies needed explore clinical applications long-term preventing complications.

Language: Английский

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Exercise-induced IL-15 acted as a positive prognostic implication and tumor-suppressed role in pan-cancer

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 17, 2022

Objective: Exercise can produce a large number of cytokines that may benefit cancer patients, including Interleukin 15 (IL-15). IL-15 is cytokine has multiple functions in regulating the adaptive and innate immune systems tumorigenesis lung breast cancers. However, roles other types remain unknown. In this article, we try to systematically analyze if potential molecular biomarker for predicting patient prognosis pan-cancer its connection with anti-cancer effects exercise. Methods: The expression was detected by Cancer Genome Atlas (TCGA) database, Human protein (HPA), Genotype Tissue-Expression (GTEX) database. Analysis genomic alterations human organic tissues analyzed cBioPortal database HPA. correlations between survival outcomes, clinical features, immune-associated cell infiltration, ferroptosis/cuproptosis were using TCGA, ESTIMATE algorithm, TIMER databases. Gene Set Enrichment (GSEA) performed evaluate biological pan-cancer. Results: differential analysis suggested level mRNA significantly downregulated 12 tumor compared normal tissues, which similar most types. high could predict positive outcome patients LUAD (lung adenocarcinoma), COAD (colon COADREAD rectum ESCA (esophageal carcinoma), SKCM (skin cutaneous melanoma), UCS (uterine carcinosarcoma), READ (rectum adenocarcinoma). Moreover, amplification found be frequent mutation type genomic. Furthermore, correlated infiltration levels various cells assessed algorithm addition, positively ferroptosis/cuproptosis-related genes (ACSL4 LIPT1) Levels reported elevated humans 10-120 min following an acute Therefore, hypothesized better regular exercise achieved IL-15. Conclusion: Our results demonstrated prognosis, immunoreaction, partly explained

Language: Английский

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Mechanisms of exercise in the treatment of lung cancer – a mini-review

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 24, 2023

Lung cancer constitutes a formidable menace to global health and well-being, as its incidence mortality rate escalate at an alarming pace. In recent years, research has indicated that exercise potential roles in both the prevention treatment of lung cancer. However, exact mechanism coordinating effect on is unclear, limiting use clinical practice. The purpose this review explore mechanisms through which exerts anticancer effects against This will analyze biological basis exercise’s cancer, with focus aspects such tumor microenvironment, matrix regulation, apoptosis angiogenesis. Finally, we discuss future directions applications.

Language: Английский

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Role of transforming growth factor-β in peripheral nerve regeneration

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(2), P. 380 - 386

Published: May 25, 2023

Abstract Injuries caused by trauma and neurodegenerative diseases can damage the peripheral nervous system cause functional deficits. Unlike in central system, damaged axons nerves be induced to regenerate response intrinsic cues after reprogramming or a growth-promoting microenvironment created Schwann cells. However, axon regeneration repair do not automatically result restoration of function, which is ultimate therapeutic goal but also major clinical challenge. Transforming growth factor (TGF) multifunctional cytokine that regulates various biological processes including tissue repair, embryo development, cell differentiation. There accumulating evidence TGF-β family proteins participate nerve through factors signaling pathways regulating transformation cells; recruiting specific immune controlling permeability blood-nerve barrier, thereby stimulating growth; inhibiting remyelination regenerated axons. has been applied treatment injury animal models. In this context, we review functions potential applications.

Language: Английский

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Role of oxidative stress in the concurrent development of osteoporosis and tendinopathy: Emerging challenges and prospects for treatment modalities

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(13)

Published: July 1, 2024

Both osteoporosis and tendinopathy are widely prevalent disorders, encountered in diverse medical contexts. Whilst each condition has distinct pathophysiological characteristics, they share several risk factors underlying causes. Notably, oxidative stress emerges as a crucial intersecting factor, playing pivotal role the onset progression of both diseases. This imbalance arises from dysregulation generating neutralising reactive oxygen species (ROS), leading to an abnormal environment. Elevated levels ROS can induce multiple cellular disruptions, such cytotoxicity, apoptosis activation reduced cell function, contributing tissue deterioration weakening structural integrity bones tendons. Antioxidants substances that prevent or slow down oxidation process, including Vitamin C, melatonin, resveratrol, anthocyanins so on, demonstrating potential treating these overlapping disorders. comprehensive review aims elucidate complex within interlinked pathways comorbid conditions. By integrating contemporary research empirical findings, our objective is outline new conceptual models innovative treatment strategies for effectively managing underscores importance further in-depth validate efficacy antioxidants traditional Chinese medicine plans, well explore targeted interventions focused on promising areas future advancements.

Language: Английский

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Endothelial cells-derived exosomes-based hydrogel improved tendinous repair via anti-inflammatory and tissue regeneration-promoting properties

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 9, 2024

Tendon injuries are common orthopedic ailments with a challenging healing trajectory, especially in cases like the Achilles tendon afflictions. The trajectory of is often suboptimal, leading to scar formation and functional impairment due inherent low metabolic activity vascularization tissue. As pressing needed for effective interventions, efforts made explore biomaterials augment healing. However, tissue engineering approaches face hurdles optimizing scaffolds nanomedical strategies. To navigate these challenges, an injectable hydrogel amalgamated human umbilical vein endothelial cells-derived exosomes (HUVECs-Exos) was prepared named H-Exos-gel this study, aiming enhance repair. In our research involving model 60 rats, we investigated efficacy through histological assessments performed at 2 4 weeks behavioral conducted 4-week mark revealed its ability tendon's mechanical strength, regulate inflammation facilitate regeneration recovery. Mechanically, modulated cellular behaviors macrophages tendon-derived stem cells (TDSCs) by inhibiting inflammation-related pathways promoting proliferation-related pathways. Our findings delineate that epitomizes viable bioactive medium healing, heralding promising avenue clinical amelioration injuries.

Language: Английский

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Extracellular Vesicles from NMN Preconditioned Mesenchymal Stem Cells Ameliorated Myocardial Infarction via miR-210-3p Promoted Angiogenesis

Stem Cell Reviews and Reports, Journal Year: 2023, Volume and Issue: 19(4), P. 1051 - 1066

Published: Jan. 25, 2023

Mesenchymal stem cell-derived extracellular vesicles (MSCs-EVs) possess cardioprotection in acute myocardial infarction. Nevertheless, the therapeutic intervention potential and molecular mechanism of EVs from NMN (Nicotinamide mononucleotide) preconditioned hUCMSCs (N-EVs) infarction remains unknown. In present study, (M-EVs) N-EVs were identified by electron microscopy, immunoblotting nanoparticle tracking analysis. Compared with M-EVs, significantly increased proliferation, migration, angiogenesis HUVECs. Meanwhile, markedly reduced apoptosis cardiac fibrosis promoted peri-infarct region MI rats. A high-throughput miRNA sequencing qPCR methods analysis revealed that miR-210-3p was abundant expression obviously upregulated HUVECs after treated. Overexpression enhanced tube formation, migration proliferative capacities However, downregulation decreased Furthermore, bioinformatics luciferase assays EphrinA3 (EFNA3) a direct target miR-210-3p. Knockdown impaired its ability to protect heart Altogether, these results indicated infarct healing through improvement via targeting EFNA3. Created Biorender.com.

Language: Английский

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Recent advances of exosomes in soft tissue injuries in sports medicine: A critical review on biological and biomaterial applications

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 364, P. 90 - 108

Published: Oct. 27, 2023

Language: Английский

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Sja-let-7 suppresses the development of liver fibrosis via Schistosoma japonicum extracellular vesicles

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(4), P. e1012153 - e1012153

Published: April 10, 2024

Schistosomiasis is a fatal zoonotic parasitic disease that also threatens human health. The main pathological features of schistosomiasis are granulomatous inflammation and subsequent liver fibrosis, which complex, chronic, progressive disease. Extracellular vesicles (EVs) derived from schistosome eggs broadly involved in host-parasite communication act as important contributors to schistosome-induced fibrosis. However, it remains unclear whether substances secreted by the EVs Schistosoma japonicum , long-term “partner” hepatic portal vein host, participate Here, we report S . worms attenuated fibrosis delivering sja-let-7 into stellate cells (HSCs). Mechanistically, activation HSCs was reduced targeting collagen type I alpha 2 chain (Col1α2) downregulation TGF-β/Smad signaling pathway both vivo vitro Overall, these results contribute further understanding molecular mechanisms underlying interactions identified sja-let-7/Col1α2/TGF-β/Smad axis potential target for treatment schistosomiasis-related

Language: Английский

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HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 12, 2024

Abstract The current first-line treatment for repairing cartilage defects in clinical practice is the creation of microfractures (MF) to stimulate release mesenchymal stem cells (MSCs); however, this method has many limitations. Recent studies have found that MSC-derived extracellular vesicles (MSC-EVs) play an important role tissue regeneration. This study aimed verify whether MSC-EVs promote damage repair mediated by MFs and explore mechanisms. In vitro experiments showed human umbilical cord Wharton’s jelly (hWJMSC-EVs) promoted vitality chondrocytes proliferation differentiation ability bone marrow-derived MSCs. was mainly because hWJMSC-EVs carry integrin beta-1 (ITGB1), MSCs overexpress ITGB1 after absorbing EVs, thereby activating transforming growth factor-β/Smad2/3 axis. a rabbit knee joint model osteochondral defect repair, injection different concentrations into cavity concentration 50 µg/ml significantly improved formation transparent MF surgery. Extraction regenerated revealed changes ITGB1, factor-β, Smad2/3 were directly proportional cartilage. summary, Graphical abstract

Language: Английский

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