MicroRNA-targeting nanomedicines for the treatment of intervertebral disc degeneration

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115214 - 115214

Published: Feb. 22, 2024

Language: Английский

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Injectable Hydrogel Based on Enzymatic Initiation of Keratin Methacrylate for Controlled Exosome Release in Intervertebral Disc Degeneration Therapy

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(32)

Published: April 5, 2024

Abstract The treatment of intervertebral disc degeneration (IVDD) using bone marrow mesenchymal stem cell‐derived exosomes has shown success in alleviating inflammation and restoring the extracellular matrix (ECM), however, challenges persist due to deficiency mechanical support controlled release. Herein, a carbon‐carbon double bond modified keratin (KeMA) is synthesized by 2‐isocyanatoethyl modification for wrapping. This injectable KeMA hydrogel, initiated biocompatible glucose/ glucose oxidase/ horse radish peroxidase enzymatic cascade reaction with acetylacetone N‐vinylpyrrolidone, displayed rapid gelation, resembling nucleus pulposus (NP) elasticity, excellent cytocompatibility. In vitro studies showcased that exosomes‐loaded hydrogel (Exo@KeMA) enhanced exosome release kinetics, suppressed inflammation, fostered (ECM) regeneration, reinstated NP biomechanics. RNA‐seq analysis indicated Exo@KeMA's effects involved PI3K‐Akt signaling regeneration NF‐κB inhibition anti‐inflammation. vivo IVDD rat models demonstrated Exo@KeMA attenuated maintained water content, preserved height, promoted structural regeneration. research introduces an as promising therapy IVDD, facilitating biomechanics restoration, anti‐inflammatory response, ECM

Language: Английский

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Hydrogel-Based Strategies for Intervertebral Disc Regeneration: Advances, Challenges and Clinical Prospects

Gels, Journal Year: 2024, Volume and Issue: 10(1), P. 62 - 62

Published: Jan. 15, 2024

Millions of people worldwide suffer from low back pain and disability associated with intervertebral disc (IVD) degeneration. IVD degeneration is highly correlated aging, as the nucleus pulposus (NP) dehydrates annulus fibrosus (AF) fissures form, which often results in herniation or space collapse related clinical symptoms. Currently available options for treating are symptoms control therapy modalities, and/or medication, surgical resection without spinal fusion. As such, there an urgent demand more effective disease-modifying treatments this ubiquitous disorder, rather than current paradigms focused only on symptom control. Hydrogels unique biomaterials that have a variety distinctive qualities, including (but not limited to) biocompatibility, adjustable mechanical characteristics, most importantly, capacity to absorb retain water manner like native human tissue. In recent years, various hydrogels been investigated vitro vivo repair discs, some ready testing. review, we summarize latest findings developments application hydrogel technology regeneration discs.

Language: Английский

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Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.

Language: Английский

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Designing hydrogel for application in spinal surgery

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101536 - 101536

Published: Feb. 3, 2025

Language: Английский

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Injectable mesoporous bioactive glass/sodium alginate hydrogel loaded with melatonin for intervertebral disc regeneration

Materials Today Bio, Journal Year: 2023, Volume and Issue: 22, P. 100731 - 100731

Published: July 17, 2023

Intervertebral disc degeneration (IDD) is a major contributing factor to both lower back and neck pain. As IDD progresses, the intervertebral (IVD) loses its ability maintain height when subjected axial loading. This failure in weight-bearing capacity of IVD characteristic feature degeneration. Natural polymer-based hydrogel, derived from biological polymers, possesses biocompatibility able mimic structure extracellular matrix, enabling them support cellular behavior. However, their mechanical performance relatively poor, thus limiting application regeneration. In this study, we developed an injectable composite namely, Mel-MBG/SA, which similar natural IVD. Mesoporous bioactive glasses not only enhance hydrogels, but also act as carriers for melatonin (Mel) suppress inflammation during IDD. The Mel-MBG/SA hydrogel further provides mixed system with sustained Mel release alleviate IL-1β-induced oxidative stress relieve associated pathology. Furthermore, our study shows that delivery can effectively rat tail model, expected promote approach presents novel strategy promoting tissue regeneration by modulating inflammatory environment while harnessing properties material.

Language: Английский

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Fish Collagen Cross‐Linking Strategies to Improve Mechanical and Bioactive Capabilities for Tissue Engineering and Regenerative Medicine

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(45)

Published: July 31, 2024

Abstract Collagen is the most abundant protein found in humans and fundamental to tissue structure function. products used biomedical research are primarily derived from mammals, despite being mainly responsible for providing strength native connective tissue, collagen hydrogels have comparatively low mechanical properties without use of additional cross‐linking strategies. Alternative sources collagen, like fish emerging as key biomaterials engineering regenerative medicine (TERM). By addressing cultural/religious concerns, ease extraction, absence mammalian‐derived allergens, retention functional motifs, has many promising characteristics that make it a suitable alternative mammalian collagen. Several physical chemical strategies explored create more stable resilient scaffolds variety TERM applications. This comprehensive review explores how these modifications optimized hydrogel systems. Herein, their reported research, well types treatments (including sterilization) alter structures functions, presented date.

Language: Английский

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From drugs to biomaterials: a review of emerging therapeutic strategies for intervertebral disc inflammation

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 30, 2024

Chronic low back pain (LBP) is an increasingly prevalent issue, especially among aging populations. A major underlying cause of LBP intervertebral disc degeneration (IDD), often triggered by (IVD) inflammation. Inflammation the IVD divided into Septic and Aseptic Conservative therapy surgical treatment fail to address root IDD. Recent advances in infection inflammation range from antibiotics small-molecule drugs cellular therapies, biological agents, innovative biomaterials. This review sheds light on complex mechanisms physiological biochemical processes Furthermore, it provides overview recent research developments this area, intending identify novel therapeutic targets guide future clinical strategies for effectively treating IVD-related conditions.

Language: Английский

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A natural hydrogel complex improves intervertebral disc degeneration by correcting fatty acid metabolism and inhibiting nucleus pulposus cell pyroptosis

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101081 - 101081

Published: May 3, 2024

The degeneration of intervertebral discs is strongly associated with the occurrence pyroptosis in nucleus pulposus (NP) cells. This characterized by abnormal metabolism fatty acids degenerative pathological state, which further exacerbated inflammatory microenvironment and degradation extracellular matrix. In order to address this issue, we have developed a fibrin hydrogel complex (FG@PEV). intricate formulation amalgamates beneficial attributes platelet extravasation vesicles, contributing tissue repair regeneration. Furthermore, showcases exceptional stability, gradual-release capabilities, high degree biocompatibility. substantiate biological significance FG@PEV disc (IVDD), conducted comprehensive investigation into its potential mechanism action through integration RNA-seq sequencing metabolomics analysis. these findings were subsequently validated experimentation both vivo vitro models. experimental results revealed that intervention possesses capability reshape within disc. It also addresses irregularities acid cells, consequently hindering cellular slowing down regulation matrix synthesis degradation. As result, injectable gel system represents promising innovative therapeutic approach for mitigating degeneration.

Language: Английский

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Application and development of hydrogel biomaterials for the treatment of intervertebral disc degeneration: a literature review

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 7, 2023

Low back pain caused by disc herniation and spinal stenosis imposes an enormous medical burden on society due to its high prevalence refractory nature. This is mainly the long-term inflammation degradation of extracellular matrix in process intervertebral degeneration (IVDD), which manifests as loss water nucleus pulposus (NP) formation fibrous fissures. Biomaterial repair strategies involving hydrogels play important role treatment degeneration. Excellent biocompatibility, tunable mechanical properties, easy modification, injectability, ability encapsulate drugs, cells, genes, etc. make good candidates scaffolds cell/drug carriers for treating NP other aspects IVDD. review first briefly describes anatomy, pathology, current treatments IVDD, then introduces different types addresses "smart hydrogels". Finally, we discuss feasibility prospects using treat

Language: Английский

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