MiR‐17‐5p‐engineered sEVs Encapsulated in GelMA Hydrogel Facilitated Diabetic Wound Healing by Targeting PTEN and p21

Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)

Published: Jan. 29, 2024

Abstract Delayed wound healing is a major complication of diabetes, and associated with impaired cellular functions. Current treatments are unsatisfactory. Based on the previous reports microRNA expression in small extracellular vesicles (sEVs), miR‐17‐5p‐engineered sEVs (sEVs 17‐OE ) encapsulated them gelatin methacryloyl (GelMA) hydrogel for diabetic wounds treatment fabricated. SEVs successfully fabricated 16‐fold increase miR‐17‐5p expression. inhibited senescence promoted proliferation, migration, tube formation high glucose‐induced human umbilical vein endothelial cells (HG‐HUVECs). Additionally, also performs promotive effect dermal fibroblasts (HG‐HDFs). Mechanism analysis showed expressions p21 phosphatase tensin homolog (PTEN), as target genes miR‐17‐5p, downregulated significantly by . Accordingly, downstream pathways PTEN, activated. Next, loaded GelMA to fabricate novel bioactive dressing evaluate their effects healing. Gel‐sEVs effectively accelerated promoting angiogenesis collagen deposition. The mechanism may be local cell proliferation. Therefore, loading hydrogel, offering an option chronic management

Language: Английский

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Neutrophil extracellular traps in homeostasis and disease

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 20, 2024

Language: Английский

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Regulation of inflammation during wound healing: the function of mesenchymal stem cells and strategies for therapeutic enhancement

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 15, 2024

A wound takes a long time to heal and involves several steps. Following tissue injury, inflammation is the primary cause of regeneration repair processes. As result, pathophysiological processes involving skin damage, healing, remodeling depend critically on control inflammation. The fact that it feasible target for improving prognosis healing has lately become clear. Mesenchymal stem cells (MSCs) are an innovative effective therapeutic option due their immunomodulatory paracrine properties. By controlling inflammatory milieu wounds through immunomodulation, transplanted MSCs have been shown speed up process. In addition other mechanisms, including handling neutrophil activity modifying macrophage polarization, there may be modifications activation T cells, natural killer (NK) dendritic (DCs). Furthermore, studies pretreating improves ability modulate immunity. this review, we summarize existing knowledge about how influence local in by influencing immunity facilitate We also provide overview optimizing techniques when used treat wounds.

Language: Английский

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Dandelion-derived vesicles-laden hydrogel dressings capable of neutralizing Staphylococcus aureus exotoxins for the care of invasive wounds

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 368, P. 355 - 371

Published: March 6, 2024

Language: Английский

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Small extracellular vesicles: Yields, functionalization and applications in diabetic wound management

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(4)

Published: Oct. 1, 2023

Abstract Diabetic wounds have imposed a significant burden on both patients and society with prolonged healing processes hindered by dysfunctional skin repair cells. Small extracellular vesicles (sEVs), as the important media for intercellular communications, show promising therapeutic potential in treating diabetic restoring cellular functions. However, low yields limited bio‐function of sEVs greatly challenge their large‐scale clinical use. Here, we briefly overview biogenesis uptake sEVs, emphasize current advances improving optimizing function engineering approaches, summarize applications engineered wound treatment. Furthermore, undissolved issues during transformation are also discussed. This critical review aims to provide meaningful guidance future management wounds.

Language: Английский

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Role and Therapeutic Targeting Strategies of Neutrophil Extracellular Traps in Inflammation

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 5265 - 5287

Published: Sept. 1, 2023

Abstract: Neutrophil extracellular traps (NETs) are large DNA reticular structures secreted by neutrophils and decorated with histones antimicrobial proteins. As a key mechanism for to resist microbial invasion, NETs play an important role in the killing of microorganisms (bacteria, fungi, viruses). Although mostly known mediating killing, increasing evidence suggests that excessive induced stimulation physical chemical components, microorganisms, pathological factors can exacerbate inflammation organ damage. This review summarizes induction focuses on strategies inhibiting NETosis mechanisms involved pathogen evasion NETs. Furthermore, herbal medicine inhibitors nanodelivery improve efficiency inhibition levels Graphical Keywords: neutrophil traps, inflammation, targeted inhibition, nanotherapy,

Language: Английский

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Multifunctional alginate/polydeoxyribonucleotide hydrogels for promoting diabetic wound healing

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 257, P. 128367 - 128367

Published: Nov. 27, 2023

Language: Английский

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circCDK13-loaded small extracellular vesicles accelerate healing in preclinical diabetic wound models

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 9, 2024

Abstract Chronic wounds are a major complication in patients with diabetes. Here, we identify therapeutic circRNA and load it into small extracellular vesicles (sEVs) to treat diabetic preclinical models. We show that circCDK13 can stimulate the proliferation migration of human dermal fibroblasts epidermal keratinocytes by interacting insulin-like growth factor 2 mRNA binding protein 3 an N6-Methyladenosine-dependent manner enhance CD44 c-MYC expression. engineered sEVs overexpress local subcutaneous injection male db/db mouse streptozotocin-induced type I rats could accelerate wound healing skin appendage regeneration. Our study demonstrates delivery may present option for treatment.

Language: Английский

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A multifunctional injectable hydrogel for boosted diabetic wound healing assisted by Quercetin-ZIF system

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 495, P. 153425 - 153425

Published: June 22, 2024

Language: Английский

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Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217

Published: Jan. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Language: Английский

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