Lipid nanoparticle-based mRNA delivery systems for cancer immunotherapy

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Aug. 7, 2023

Abstract Cancer immunotherapy, which harnesses the power of immune system, has shown immense promise in fight against malignancies. Messenger RNA (mRNA) stands as a versatile instrument this context, with its capacity to encode tumor-associated antigens (TAAs), cell receptors, cytokines, and antibodies. Nevertheless, inherent structural instability mRNA requires development effective delivery systems. Lipid nanoparticles (LNPs) have emerged significant candidates for cancer providing both protection enhanced intracellular efficiency. In review, we offer comprehensive summary recent advancements LNP-based systems, focus on strategies optimizing design mRNA-encoded therapeutics treatment. Furthermore, delve into challenges encountered field contemplate future perspectives, aiming improve safety efficacy immunotherapies. Graphical

Language: Английский

---

Cell Surface Engineering Tools for Programming Living Assemblies

Advanced Science, Journal Year: 2023, Volume and Issue: 10(34)

Published: Oct. 12, 2023

Breakthroughs in precision cell surface engineering tools are supporting the rapid development of programmable living assemblies with valuable features for tackling complex biological problems. Herein, authors overview most recent technological advances chemically- and biologically-driven toolboxes mammalian surfaces triggering their assembly into architectures. A particular focus is given to technologies enabling biomimetic cell-cell social interactions multicellular cell-sorting events. Further advancements modification may expand currently available bioengineering toolset unlock a new generation personalized therapeutics clinically relevant biofunctionalities. The combination state-of-the-art modifications advanced biofabrication envisioned contribute toward generating materials increasing tissue/organ-mimetic bioactivities therapeutic potential.

Language: Английский

---

Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 333, P. 103301 - 103301

Published: Sept. 6, 2024

Language: Английский

---

Lipid anchor-mediated NK cell surface engineering for enhanced cancer immunotherapy

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 473, P. 145211 - 145211

Published: Aug. 6, 2023

Language: Английский

---

Surface Engineering of Natural Killer Cells with CD44‐targeting Ligands for Augmented Cancer Immunotherapy

Small, Journal Year: 2023, Volume and Issue: 20(24)

Published: Dec. 31, 2023

Abstract Adoptive immunotherapy utilizing natural killer (NK) cells has demonstrated remarkable efficacy in treating hematologic malignancies. However, its clinical intervention for solid tumors is hindered by the limited expression of tumor‐specific antigens. Herein, lipid‐PEG conjugated hyaluronic acid (HA) materials (HA‐PEG‐Lipid) simple ex‐vivo surface coating NK developed 1) lipid‐mediated cellular membrane anchoring via hydrophobic interaction and thereby 2) sufficient presentation CD44 ligand (i.e., HA) onto cancer targeting, without need genetic manipulation. Membrane‐engineered can selectively recognize CD44‐overexpressing through HA‐CD44 affinity subsequently induce situ activation elimination. Therefore, surface‐engineered using HA‐PEG‐Lipid (HANK cells) establish an immune synapse with MIA PaCa‐2 pancreatic cells, triggering “recognition‐activation” mechanism, ultimately eliminating cells. Moreover, mouse xenograft tumor models, administrated HANK demonstrate significant infiltration into tumors, resulting apoptosis/necrosis effective suppression progression metastasis, as compared to gemcitabine. Taken together, biomaterials expedite treatment facilitating a sequential recognition‐activation mechanism suggesting promising approach cell‐mediated immunotherapy.

Language: Английский

---

Polymeric biomaterial-inspired cell surface modulation for the development of novel anticancer therapeutics

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Immune cell-based therapies are a rapidly emerging class of new medicines that directly treat and prevent targeted cancer. However multiple biological barriers impede the activity live immune cells, therefore necessitate use surface-modified cells for cancer prevention. Synthetic and/or natural biomaterials represent leading approach cell surface modulation. Different types can be applied to membranes through hydrophobic insertion, layer-by-layer attachment, covalent conjugations acquire modification in mammalian cells. These generate reciprocity enable cell–cell interactions. In this review, we highlight different (lipidic polymeric)-based advanced applications cell–surface modulation, few recognition moieties, how their interplay interaction. We discuss cancer-killing efficacy NK followed by engineering treatment. Ultimately, review connects biologically active play key roles immunotherapy applications.

Language: Английский

---

Optimized Design of Hyaluronic Acid–Lipid Conjugate Biomaterial for Augmenting CD44 Recognition of Surface-Engineered NK Cells

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(3), P. 1959 - 1971

Published: Feb. 20, 2024

Triple-negative breast cancer (TNBC) presents treatment challenges due to a lack of detectable surface receptors. Natural killer (NK) cell-based adaptive immunotherapy is promising because the characteristic anticancer effects killing malignant cells directly by secreting cytokines and lytic granules. To maximize recognition ability NK cells, biomaterial-mediated ex vivo cell engineering has been developed for sufficient membrane immobilization tumor-targeting ligands via hydrophobic anchoring. In this study, we optimized amphiphilic balances coating materials composed CD44-targeting hyaluronic acid (HA)-poly(ethylene glycol) (PEG)-lipid improve TNBC effect. Changes in modular design our material differentiating hydrophilic PEG length incorporating lipid amount into HA backbones precisely regulated nature HA-PEG-lipid conjugates. The biomaterial demonstrated improved anchoring membranes facilitating presentation level onto surfaces. This led enhanced targeting increasing formation immune synapse, thereby augmenting capability specifically toward CD44-positive cells. Our approach addresses solid tumors with deficiency tumor-specific antigens while offering valuable strategy using balance techniques.

Language: Английский

---

Engineered inulin-based hybrid biomaterials for augmented immunomodulatory responses

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 340, P. 122311 - 122311

Published: May 23, 2024

Language: Английский

---

Tailoring tumor-recognizable hyaluronic acid–lipid conjugates to enhance anticancer efficacies of surface-engineered natural killer cells

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Dec. 14, 2023

Abstract Natural killer (NK) cells have clinical advantages in adoptive cell therapy owing to their inherent anticancer efficacy and ability identify eliminate malignant tumors. However, insufficient cancer-targeting ligands on NK surfaces often inhibit immunotherapeutic performance, especially immunosuppressive tumor microenvironment. To facilitate recognition subsequent function of cells, we developed hyaluronic acid (HA, target CD44 overexpressed onto cancer cells)-poly(ethylene glycol) (PEG, cytoplasmic penetration blocker)-Lipid (molecular anchor for membrane decoration through hydrophobic interaction) conjugates biomaterial-mediated ex vivo surface engineering. Among these major compartments (i.e., Lipid, PEG HA), optimization lipid anchors (in terms chemical structure intrinsic amphiphilicity) is the most important design parameter modulate interaction with dynamic membranes. Here, three different types including 1,2-dimyristoyl-sn-glycero-3-phosphati-dylethanolamine (C14:0), 1,2-distearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE, C18:0), cholesterol were evaluated maximize coating associated performance surface-engineered (HALipid-NK cells). Our results demonstrated that coated HA-PEG-DSPE exhibited significantly enhanced efficacies toward MDA-MB-231 breast without an off-target effect human fibroblasts specifically via increased prolonged duration HA surfaces, thereby improving HA-CD44 recognition. These suggest our HALipid-NK tumor-recognizable could be further utilized various immunotherapies. Graphical

Language: Английский

---

Biomaterial-Mediated Exogenous Facile Coating of Natural Killer Cells for Enhancing Anticancer Efficacy toward Hepatocellular Carcinoma

Bioconjugate Chemistry, Journal Year: 2023, Volume and Issue: 34(10), P. 1789 - 1801

Published: Sept. 20, 2023

Natural killer (NK) cells exhibit a good therapeutic efficacy against various malignant cancer cells. However, the of plain NK is relatively low due to inadequate selectivity for Therefore, enhance targeting and anticancer cells, we have rationally designed biomaterial-mediated ex vivo surface engineering technique membrane decoration recognition ligands onto Our lipid conjugate biomaterial contains three major functional moieties: (1) 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) cell anchoring, (2) polyethylene glycol intracellular penetration blocker, (3) lactobionic acid (LBA) recognition. The was successfully applied surfaces (LBA-NK) functionalities, especially toward asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma. Highly efficient homogeneous editing achieved with simple coating process while maintaining intrinsic properties LBA-NK showed potential ASGPR-mediated tumor binding (through LBA-ASGPR interaction) thereby significantly augmented efficacies HepG2 liver Thus, can be novel strategy treatment cancers via facilitated immune synapse interactions in comparison currently available therapies.

Language: Английский

---