A metamaterial scaffold beyond modulus limits: enhanced osteogenesis and angiogenesis of critical bone defects

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Metallic scaffolds have shown promise in regenerating critical bone defects. However, limitations persist achieving a modulus below 100 MPa due to insufficient strength. Consequently, the osteogenic impact of lower and greater tissue strain ( > 1%) remains unclear. Here, we introduce metamaterial scaffold that decouples strength through two-stage deformation. The facilitates an effective only 13 MPa, ensuring adaptability during regeneration. Followed by stiff stage, it provides necessary for load-bearing requirements. In vivo, induces 2% callus strain, upregulating calcium channels HIF-1α enhance osteogenesis angiogenesis. 4-week histomorphology reveals 44% 498% increase new fraction versus classic with 500 modulus, respectively. This design transcends traditional modulus-matching paradigms, prioritizing Its tunable mechanical properties also present promising implications advancing mechanisms addressing clinical challenges.

Language: Английский

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Mild photothermal therapy assist in promoting bone repair: Related mechanism and materials

Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100834 - 100834

Published: Oct. 20, 2023

Achieving precision treatment in bone tissue engineering (BTE) remains a challenge. Photothermal therapy (PTT), as form of therapy, has been extensively investigated for its safety and efficacy. It demonstrated significant potential the orthopedic diseases such tumors, postoperative infections osteoarthritis. However, high temperatures associated with PTT can lead to certain limitations drawbacks. In recent years, researchers have explored use biomaterials mild photothermal (MPT), which offers promising approach addressing these limitations. This review provides comprehensive overview mechanisms underlying MPT presents compilation agents their utilization strategies repair. Additionally, paper discusses future prospects MPT-assisted regeneration, aiming provide insights recommendations optimizing material design this field.

Language: Английский

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Metal-organic frameworks functionalized biomaterials for promoting bone repair

Materials Today Bio, Journal Year: 2023, Volume and Issue: 21, P. 100717 - 100717

Published: June 27, 2023

Bone defects induced by bone trauma, tumors and osteoarthritis greatly affect the life quality health of patients. The biomaterials with numerous advantages are becoming most preferred options for repairing treating orthopedic diseases. However, their effects remains unsatisfactory, especially in suffering from tumor, inflammation, and/or bacterial infection. There several strategies to functionalize biomaterials, but a more general efficient method is essential accomplishing functionalization biomaterials. Possessing high specific surface, porosity, controlled degradability variable composition, metal-organic frameworks (MOFs) materials inherently advantageous functionalizing tremendous improvements having been achieved. This review summarizes recent progresses MOFs functionalized promoting repair therapeutic effects. In specific, utilizing various properties diverse materials, integrated achieve enhanced regeneration, antibacterial, anti-inflammatory anti-tumor functions. Finally, summary prospects on development MOFs-functionalized were discussed.

Language: Английский

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3D‐printed degradable hydroxyapatite bioactive ceramics for skull regeneration

MedComm – Biomaterials and Applications, Journal Year: 2023, Volume and Issue: 2(2)

Published: June 1, 2023

Abstract Hydroxyapatite (HA) bioceramics have been extensively employed as bone tissue scaffolds owing to their biodegradability and osteoinductivity. In our work, HA, a significant component of natural used the raw material produce porous employing three‐dimensional (3D)‐printing technology. Physical chemical properties, porosity, compression resistance were investigated in vitro. The scaffold was confirmed large number interconnected pore structures on surface inside HA showed good cell compatibility adhesion text. To analyze effect repair regeneration vivo, large‐size defect beagle skull repaired with 3D printing group an autologous (ABG) for 8 months. Images histological analysis indicated better integration adjacent tissues. However, there obvious gaps ABG, which indicates weak ability this due unmatched implant dimension. Immunohistochemistry immunofluorescence results that 3D‐printed had highly vascularized structure. This study are osteoinductivity biodegradable great potential maxillofacial regeneration.

Language: Английский

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Advanced Ti–Nb–Ta Alloys for Bone Implants with Improved Functionality

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(2), P. 46 - 46

Published: Feb. 17, 2024

The additive manufacturing of titanium–niobium–tantalum alloys with nominal chemical compositions Ti–xNb–6Ta (x = 20, 27, 35) by means laser beam powder bed fusion is reported, and their potential as implant materials elaborated mechanical biological characterization. properties dense specimens manufactured in different build orientations open porous Ti–20Nb–6Ta are evaluated. Compression tests indicate that strength elasticity influenced the composition orientation. minimum always observed 90° It lowest for (43.2 ± 2.7 GPa) can be further reduced to 8.1 1.0 GPa (p < 0.001). Furthermore, human osteoblasts cultivated 7 14 days on as-printed response compared Ti–6Al–4V. Build orientation cultivation time significantly affect gene expression profile osteogenic differentiation markers. Incomplete cell spreading 0° orientation, whereas widely stretched cells i.e., parallel direction. Compared Ti–6Al–4V, Ti–Nb–Ta promote improved osteogenesis reduce induction inflammation. Accordingly, have favorable great application orthopedic implants.

Language: Английский

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Constructing osteo-inductive bio-ink for 3D printing through hybridization of gelatin with maleic acid modified bacterial cellulose by regulating addition volumes of maleic acid solution

Journal of Bioresources and Bioproducts, Journal Year: 2024, Volume and Issue: 9(3), P. 336 - 350

Published: April 4, 2024

Bacterial cellulose (BC) is an exopolysaccharide with unique properties that has been applied in various fields. However, the dense and intertwined nature of BC fibers limits its use certain applications, including 3D printing scaffolds for bone regeneration. In this work, a controllable BC-based bio-ink was successfully prepared by modifying neat through maleic acid (MA) treatment, aiming to promote tissue To achieve homogeneous dispersions while preserving crystalline chemical properties, modified MA solution (60%, w/V) solid-liquid ratio from 1꞉5 1꞉50 (w/V) obtain MA-BC dispersions. The analysis results microstructure, group, crystallinity, wettability indicated BC/MA 1꞉30 demonstrated best pre-treatment performance MA-BC. Subsequently, combining gelatin, we formulated MA-BC-GEL gels favorable rheological compression modulus, which can be used as promising bio-inks bioprinting applications. vitro tests possessed excellent biocompatibility, significant ability express alkaline phosphatase gene osteogenic-related genes, facilitated formation mineralized nodules. utilization novel scaffold preparation regeneration highlights application engineering field.

Language: Английский

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Injectable hydrogels for bone regeneration with tunable degradability via peptide chirality modification

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(18), P. 4367 - 4377

Published: Jan. 1, 2024

The degradability of hydrogels plays a pivotal role in bone regeneration, yet its precise effects on the repair process remain poorly understood. Traditional studies have been limited by use with insufficient variation degradation properties for thorough comparative analysis. Addressing this gap, our study introduces development matrix metalloproteinase (MMP)-responsive engineered tunable rate, specifically designed regeneration applications. These innovative are synthesized integrating MMP-sensitive peptides, which exhibit chirality-transferred amino acids, norbornene (NB)-modified 8-arm polyethylene glycol (PEG) macromers to form hydrogel network. behavior these is manipulated through chirality incorporated resulting classification into L, LD, and D hydrogels. Remarkably, L variant shows significantly enhanced both

Language: Английский

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Design of Highly Adhesive Urchin‐Like Gold Nanostructures for Effective Topical Drug Administration and Symptomatic Relief of Corneal Dryness

Small Structures, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Topical instillation of cyclosporin A (an anti‐inflammatory drug) is clinically recommended as the first‐line treatment for dry eye disease. Nevertheless, it suffers from poor ocular drug retention and cannot comprehensively address corneal dryness‐related symptoms such oxidative stress, angiogenesis, neurodegeneration. Inspired by nanotechnology‐mediated material/biological interactions, in this study, a highly adhesive metallic nanoplatform with an urchin‐like structure designed topical quercetin administration therapy. The gold nanostructures most pronounced branch lengths exhibit strongest cytoadhesion bioadhesion capabilities, which significantly enhance nano‐urchins 150‐fold at 7 days post‐instillation compared smooth‐surfaced nanoparticles. In rabbit model dryness, single‐dose nanoformulation (high quercetin‐functionalized (NU‐Q(H))) demonstrated remarkable efficacy stimulating tear production (30‐fold improvement), inhibiting inflammatory IL‐6 expression (49‐fold attenuating pathological angiogenesis (32‐fold promoting nerve regeneration (18‐fold improvement) to high nanoparticles (NP‐Q(H)). particular, integrity surface, film, meibomian gland restored levels similar those healthy rabbits. These findings suggest promising potential nanobiomaterial structural engineering developing nanomedicines long‐acting eye‐drop formulations disease applications.

Language: Английский

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Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2512 - 2512

Published: Feb. 21, 2024

Bone differentiation is crucial for skeletal development and maintenance. Its dysfunction can cause various pathological conditions such as rickets, osteoporosis, osteogenesis imperfecta, or Paget's disease. Although traditional two-dimensional cell culture systems have contributed significantly to our understanding of bone biology, they fail replicate the intricate biotic environment tissue. Three-dimensional (3D) spheroid cultures gained widespread popularity addressing defects. This review highlights advantages employing 3D investigate differentiation. It their capacity mimic complex in vivo cellular interactions pivotal homeostasis. The exploration models research offers enhanced physiological relevance, improved predictive capabilities, reduced reliance on animal models, which advancement safer more effective strategies drug development. Studies highlighted transformative potential expanding biology developing targeted therapeutic interventions bone-related disorders. explores how demonstrated promise unraveling mechanisms governing homeostasis responses pharmacological agents.

Language: Английский

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Engineering Stimuli‐Responsive Materials for Precision Medicine

Small, Journal Year: 2024, Volume and Issue: 21(1)

Published: Oct. 23, 2024

Abstract Over the past decade, precision medicine has garnered increasing attention, making significant strides in discovering new therapeutic drugs and mechanisms, resulting notable achievements symptom alleviation, pain reduction, extended survival rates. However, limited target specificity of primary inter‐individual differences have often necessitated high‐dosage strategies, leading to challenges such as restricted deep tissue penetration rates systemic side effects. Material science advancements present a promising avenue for these issues. By leveraging distinct internal features diseased regions application specific external stimuli, responsive materials can be tailored achieve targeted delivery, controllable release, biochemical reactions. This review aims highlight latest stimuli‐responsive their potential medicine. Initially, we introduce disease‐related stimuli capable elucidating reaction principles functional groups. Subsequently, provide detailed analysis representative pre‐clinical across various clinical applications, including enhancements treatment cancers, injury diseases, inflammatory infection high‐throughput microfluidic biosensors. Finally, discuss some challenges, off‐target effects, long‐term impacts nano‐materials, ethical concerns, offer insights into future perspectives materials.

Language: Английский

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