Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 5, 2024
Abstract
Reproductive
health
concerns
like
Mayer‐Rokitansky‐Küster‐Hauser
(MRKH)
syndrome
are
prevalent
in
today's
society.
MRKH
is
a
condition
that
severely
affects
women's
sexual
life,
fertility,
and
mental
has
high
prevalence
of
one
out
5000
female
births.
Vaginoplasty
the
primary
method
to
regain
patients’
reproductive
health.
However,
conventional
vaginoplasty
faces
various
challenges,
including
complex
non‐customized
treatment
procedures
causing
intense
pains
complications.
To
bring
new
advances
vaginoplasty,
3D‐printed
hydrogel
scaffold
developed
provide
satisfactory
mechanical
support
bioactivity
for
accelerating
defect
repair
after
surgery.
The
consisting
gelatin
methacryloyl
(gelMA)
carrageenan
(Car)
custom
using
an
ambient
temperature
printing
system.
Furthermore,
undergoes
dual‐crosslinking
through
chemical
crosslinking
gelMA
ionic
Car
with
magnesium
ions
(Mg
2+
).
This
strategy
substantially
improves
overall
properties
introduces
bioactive
Mg
.
sustained
release
plus
extracts
from
dual‐crosslinked
significantly
promotes
cell
proliferation,
migration
angiogenesis.
In
preclinical
rat
model
penetrating
genital
tract
defects
mimicking
implantation
repairs
wounds
near‐normal
levels
within
week,
showing
potential
as
alternative
better
regaining
Microsystems & Nanoengineering,
Journal Year:
2023,
Volume and Issue:
9(1)
Published: Aug. 15, 2023
Conventional
manufacturing
techniques
to
fabricate
microfluidic
chips,
such
as
soft
lithography
and
hot
embossing
process,
have
limitations
that
include
difficulty
in
preparing
multiple-layered
structures,
cost-
labor-consuming
fabrication
low
productivity.
Digital
light
processing
(DLP)
technology
has
recently
emerged
a
cost-efficient
microfabrication
approach
for
the
3D
printing
of
chips;
however,
resolution
microchannels
is
still
limited
sub-100
microns
at
best.
Here,
we
developed
an
innovative
DLP
strategy
high
scalable
microchannel
by
dosing-
zoning-controlled
vat
photopolymerization
(DZC-VPP).
Specifically,
proposed
modified
mathematical
model
precisely
predict
accumulated
UV
irradiance
resin
photopolymerization,
thereby
providing
guidance
with
enhanced
resolution.
By
fine-tuning
parameters,
including
optical
irradiance,
exposure
time,
projection
region,
step
distance,
can
tailor
penetration
stemming
from
neighboring
layers,
preventing
channel
blockage
due
overexposure
or
compromised
bonding
stability
owing
insufficient
curing.
Remarkably,
this
allow
preparation
cross-sectional
dimensions
20
μm
×
using
commercial
printer
pixel
size
10
μm;
significantly
higher
than
previous
reports.
In
addition,
method
enable
biocompatible
drop-maker
units
be
used
cell
encapsulation.
general,
current
DZC-VPP
major
advances
precise
represents
significant
forward
widespread
applications
microfluidics-based
biomedical
fields.
Materials & Design,
Journal Year:
2023,
Volume and Issue:
227, P. 111705 - 111705
Published: Feb. 6, 2023
The
reconstruction
of
large
bone
defects
by
bioactive
materials
without
exogenous
cells,
cytokines
or
growth
factors
remains
a
substantial
clinical
challenge.
Herein,
we
present
the
rational
design
injectable
and
adhesive
(GMAD/LP)
hydrogels
composed
gelatin-methacryloyl
(GelMA),
dopamine-grafted
alginate
(AD),
polydopamine-functionalized
Laponite
([email
protected])
nanosheets
that
can
act
as
osteoimmune
regulators
to
engineer
pro-regenerative
microenvironment
for
personalized
regeneration.
as-fabricated
GMAD/LP
hydrogel
with
excellent
tissue
adhesion,
self-healing,
injectability,
improved
mechanical
strength
fill
irregular
in
minimally
invasive
manner.
Moreover,
exhibited
outstanding
osteogenic
potential
osteoimmunomodulatory
functions
vitro,
significantly
promoting
proliferation,
spreading,
differentiation
mesenchymal
stem
cells
(BMSCs),
which
was
further
augmented
hydrogel-induced
macrophage
M2-phenotype
polarization
effective
M2
macrophage-BMSC
crosstalk.
In
vivo
experiments
cranial
defect
model
demonstrated
induced
reparative
similar
normal
cranium,
characterized
an
increased
percentage
anti-inflammatory
macrophages
endogenous
high-level
neovascularization,
consequently
accelerating
healing.
This
strategy
not
only
achieves
but
also
provides
feasible
methodology
recover
injured
through
integrated
diverse
functions.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(33)
Published: March 29, 2024
Abstract
Collagen
crosslinking
employing
ultraviolet
A
rays
and
riboflavin
(UVA/R)
has
emerged
as
a
pivotal
technique
in
clinical
therapies,
especially
ophthalmology
since
the
1990s.
Despite
its
adoption,
lack
of
clarity
detailed
mechanism
imperative
for
refined
manufacturing
process
necessitates
further
investigation.
This
study
advances
understanding
UVA/R
crosslinked
collagen,
concentrating
on
identifying
primary
sites
using
seven
synthetic
peptides
exploring
pathways
riboflavin‐mediated
crosslinking.
The
results
demonstrate
that
tyrosine
residues
are
key
sites,
plays
dual
role
both
catalyst
competitive
inhibitor
process.
Furthermore,
collagen
matrix
exhibits
more
harmonious
balance
between
stability
degradability
compared
with
chemically
matrices,
coupled
superior
mechanical
properties
augmented
biocompatibility.
In
vivo
experiments
validate
excellent
biocompatibility,
reduced
tissue
inflammation,
promotion
regeneration.
research
provides
crucial
insights
into
mechanisms,
paving
way
development
sophisticated
collagen‐based
biomaterials
tailored
biomedical
applications.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 5, 2024
Abstract
Reproductive
health
concerns
like
Mayer‐Rokitansky‐Küster‐Hauser
(MRKH)
syndrome
are
prevalent
in
today's
society.
MRKH
is
a
condition
that
severely
affects
women's
sexual
life,
fertility,
and
mental
has
high
prevalence
of
one
out
5000
female
births.
Vaginoplasty
the
primary
method
to
regain
patients’
reproductive
health.
However,
conventional
vaginoplasty
faces
various
challenges,
including
complex
non‐customized
treatment
procedures
causing
intense
pains
complications.
To
bring
new
advances
vaginoplasty,
3D‐printed
hydrogel
scaffold
developed
provide
satisfactory
mechanical
support
bioactivity
for
accelerating
defect
repair
after
surgery.
The
consisting
gelatin
methacryloyl
(gelMA)
carrageenan
(Car)
custom
using
an
ambient
temperature
printing
system.
Furthermore,
undergoes
dual‐crosslinking
through
chemical
crosslinking
gelMA
ionic
Car
with
magnesium
ions
(Mg
2+
).
This
strategy
substantially
improves
overall
properties
introduces
bioactive
Mg
.
sustained
release
plus
extracts
from
dual‐crosslinked
significantly
promotes
cell
proliferation,
migration
angiogenesis.
In
preclinical
rat
model
penetrating
genital
tract
defects
mimicking
implantation
repairs
wounds
near‐normal
levels
within
week,
showing
potential
as
alternative
better
regaining
