The Future Exploring of Gut Microbiome-Immunity Interactions: From In Vivo/Vitro Models to In Silico Innovations DOI Creative Commons
Sara Bertorello,

Francesco Cei,

Dorian Fink

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1828 - 1828

Published: Sept. 4, 2024

Investigating the complex interactions between microbiota and immunity is crucial for a fruitful understanding progress of human health disease. This review assesses animal models, next-generation in vitro silico approaches that are used to decipher microbiome-immunity axis, evaluating their strengths limitations. While models provide comprehensive biological context, they also raise ethical practical concerns. Conversely, modern reduce involvement but require specific costs materials. When considering environmental impact these emerge as promising resource reduction, robust experimental validation ongoing refinement. Their potential significant, paving way more sustainable future research.

Language: Английский

The Challenge of Applications of Probiotics in Gastrointestinal Diseases DOI Creative Commons

William Wolfe,

Ze Xiang, Xi Yu

et al.

Advanced Gut & Microbiome Research, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 10

Published: Jan. 18, 2023

Gastrointestinal disease is characterized by gastrointestinal dysfunction with dysbiosis of the microbiome. Probiotics may act as biological agents in treating diseases through modifying gut microbiota. However, several challenges, including safety, stress resistance, postcolonization quantification, and evaluation models, hinder application probiotics diseases. This review introduces emerging methods for delivering well available materials. Furthermore, we elucidated bacteriocins their role helping obtain a competitive advantage over other strains challenges large-scale application. Bacteriocins produced also showed promising efficacy capacity immune stimulation, intestinal barrier protection, cytotoxicity against tumorigenesis. For quantification complex microbiomes probiotic encapsulated delivery systems, recent fluorescent labeling technology various vitro vivo models were reviewed. Given widespread use microecological therapy diseases, further understanding multiple updated to improve colonization system great significance live biotherapeutics.

Language: Английский

Citations

45
iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies DOI Creative Commons
Victoria Palasantzas, Isabel Tamargo-Rubio, Kieu T. T. Le

et al.

Trends in Genetics, Journal Year: 2023, Volume and Issue: 39(4), P. 268 - 284

Published: Feb. 5, 2023

Genome-wide association studies (GWAS) have now correlated hundreds of genetic variants with complex diseases and drug efficacy. Functional characterization these factors remains challenging, particularly because the lack human model systems. Molecular nanotechnological advances, in particular ability to generate patient-specific PSC lines, differentiate them into diverse cell types, seed combine on microfluidic chips, led establishment organ-on-a-chip (OoC) platforms that recapitulate organ biology. OoC technology thus provides unique personalized for studying effects host genetics environmental physiology. In this review we describe provide examples how OoCs may be used disease modeling pharmacogenetic research.

Language: Английский

Citations

41
Microfluidic Gut-on-a-Chip: Fundamentals and Challenges DOI Creative Commons

Dimple Palanilkunnathil Thomas,

Jun Zhang, Nam‐Trung Nguyen

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(1), P. 136 - 136

Published: Jan. 13, 2023

The human gut is responsible for food digestion and absorption. Recently, growing evidence has shown its vital role in the proper functioning of other organs. Advances microfluidic technologies have made a significant impact on biomedical field. Specifically, organ-on-a-chip technology (OoC), which become popular substitute animal models, capable imitating complex systems vitro been used to study pathology pharmacology. Over past decade, reviews published focused more applications prospects gut-on-a-chip (GOC) technology, but challenges solutions these limitations were often overlooked. In this review, we cover physiology review engineering approaches GOC. Fundamentals GOC models including materials fabrication, cell types, stimuli microbiota are thoroughly reviewed. We discuss present model applications, challenges, possible technology.

Language: Английский

Citations

36
In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations DOI Creative Commons

Yuli Qi,

Leilei Yu, Fengwei Tian

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 270, P. 127336 - 127336

Published: Feb. 16, 2023

Language: Английский

Citations

31
Gut-on-a-Chip Models: Current and Future Perspectives for Host–Microbial Interactions Research DOI Creative Commons
Moran Morelli, Dorota Kurek, Chee Ping Ng

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(2), P. 619 - 619

Published: Feb. 18, 2023

The intestine contains the largest microbial community in human body, gut microbiome. Increasing evidence suggests that it plays a crucial role maintaining overall health. However, while many studies have found correlation between certain diseases and changes microbiome, impact of different compositions on mechanisms by which they contribute to disease are not well understood. Traditional pre-clinical models, such as cell culture or animal limited their ability mimic complexity physiology. New mechanistic organ-on-a-chip, being developed address this issue. These models provide more accurate representation physiology could help bridge gap clinical studies. Gut-on-chip allow researchers better understand underlying effect gut. They can move field from causation accelerate development new treatments for associated with This review will discuss current future perspectives gut-on-chip study host-microbial interactions.

Language: Английский

Citations

25
Light-activated nanoclusters with tunable ROS for wound infection treatment DOI Creative Commons

Xin Wang,

Jianing Ding,

Xiao Chen

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 41, P. 385 - 399

Published: July 30, 2024

Infected wounds pose a significant clinical challenge due to bacterial resistance, recurrent infections, and impaired healing. Reactive oxygen species (ROS)-based strategies have shown promise in eradicating infections. However, the excess ROS infection site after treatments may cause irreversible damage healthy tissues. To address this issue, we developed bovine serum albumin-iridium oxide nanoclusters (BSA-IrO

Language: Английский

Citations

10
Gut microbiota in health and disease: advances and future prospects DOI Creative Commons
Y J Zhang, Hong Wang, Yingpeng Sang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Nov. 20, 2024

Abstract The gut microbiota plays a critical role in maintaining human health, influencing wide range of physiological processes, including immune regulation, metabolism, and neurological function. Recent studies have shown that imbalances composition can contribute to the onset progression various diseases, such as metabolic disorders (e.g., obesity diabetes) neurodegenerative conditions Alzheimer's Parkinson's). These are often accompanied by chronic inflammation dysregulated responses, which closely linked specific forms cell death, pyroptosis ferroptosis. Pathogenic bacteria trigger these death pathways through toxin release, while probiotics been found mitigate effects modulating responses. Despite insights, precise mechanisms influences diseases remain insufficiently understood. This review consolidates recent findings on impact immune‐mediated inflammation‐associated conditions. It also identifies gaps current research explores potential advanced technologies, organ‐on‐chip models microbiome–gut–organ axis, for deepening our understanding. Emerging tools, single‐bacterium omics spatial metabolomics, discussed their promise elucidating microbiota's disease development.

Language: Английский

Citations

10
Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease DOI Creative Commons
Alexandra Wheeler, Verena Stoeger, Róisı́n M. Owens

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1266 - 1292

Published: Jan. 1, 2024

Further development of lab-on-chip platforms is required to create an environment capable hosting more complex microbiota and immune cells.

Language: Английский

Citations

9
3D Bioprinting for Engineered Tissue Constructs and Patient‐Specific Models: Current Progress and Prospects in Clinical Applications DOI
Sang Jin Lee, Wonwoo Jeong, Anthony Atala

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Advancements in bioprinting technology are driving the creation of complex, functional tissue constructs for use engineering and regenerative medicine. Various methods, including extrusion, jetting, light-based bioprinting, have their unique advantages drawbacks. Over years, researchers industry leaders made significant progress enhancing techniques materials, resulting production increasingly sophisticated constructs. Despite this progress, challenges still need to be addressed achieving clinically relevant, human-scale constructs, presenting a hurdle widespread clinical translation. However, with ongoing interdisciplinary research collaboration, field is rapidly evolving holds promise personalized medical interventions. Continued development refinement technologies potential address complex needs, enabling functional, transplantable tissues organs, as well advanced vitro models.

Language: Английский

Citations

9
Modelling host–microbiome interactions in organ-on-a-chip platforms DOI
Yong Cheol Shin, Nam Than, So‐Youn Min

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 2(2), P. 175 - 191

Published: Nov. 17, 2023

Language: Английский

Citations

22