Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin‐Activated Platelet‐Rich Plasma Self‐Assembly

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Platelet‐rich plasma (PRP) is characterized by elevated concentrations of growth factors that facilitate bone repair. Nonetheless, the effective integration PRP with implants and sustained release its active constituents pose significant challenges. In this study, thrombin grafted onto surface polyetheretherketone (PEEK) via an N,N'‐Disuccinimidyl Carbonate (DSC) linker retained enzymatic activity enables controlled activation self‐assembly, resulting in formation a functional bio‐gel layer. The optimal concentration to be 100 U/ mL −1 determined, at which point both grafting amount reaches their peak, no further increases observed higher concentrations. solutions varying platelet enrichment ratios are subsequently activated on thrombin‐grafted PEEK surface, yielding self‐assembled bio‐gels capable factor for up 16 days. thrombin‐activated not only enhances vitro cell adhesion, proliferation, osteogenic differentiation, vascularization specific polarization macrophages, but also effectively facilitates vivo angiogenesis, immunomodulation dose‐dependent manner. Consequently, gel presents promising strategy biological functionalization orthopedic applications.

Language: Английский

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Electrospun Biomimetic Periosteum Promotes Diabetic Bone Defect Regeneration through Regulating Macrophage Polarization and Sequential Drug Release

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The inadequate vascularization and abnormal immune microenvironment in the diabetic bone defect region present a significant challenge to osteogenic regulation. Inspired by distinctive characteristics of healing staged defects structure–function relationship natural periosteum, we fabricated an electrospun bilayer biomimetic periosteum (Bilayer@E) promote regeneration defects. Here, inner layer was using coaxial electrospinning fibers, with shell incorporating zinc oxide nanoparticles (ZnO NPs) core containing silicon dioxide (SiO2 mimicking cambium periosteum; outer consisted randomly aligned fibers loaded deferoxamine (DFO), simulating fibrous finally, epigallocatechin-3-gallate (EGCG) coated onto membrane obtain Bilayer@E. presence EGCG on Bilayer@E surface efficiently triggers phenotypic transition macrophages, shifting them from M1 proinflammatory state M2 anti-inflammatory state. Moreover, sequential release ZnO NPs, DFO, SiO2 NPs exhibits antimicrobial while coordinating angiogenesis promoting mineralization cells. Importantly, shows strong vivo tissue periosteal properties rats. integration drug immunomodulation, tailored meet specific requirements during regeneration, offers new insights for advancing application biomaterials this field.

Language: Английский

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Addressing the challenges of infectious bone defects: a review of recent advances in bifunctional biomaterials

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 29, 2025

Infectious bone defects present a substantial clinical challenge due to the complex interplay between infection control and regeneration. These often result from trauma, autoimmune diseases, infections, or tumors, requiring nuanced approach that simultaneously addresses promotes tissue repair. Recent advances in engineering materials science, particularly nanomaterials nano-drug formulations, have led development of bifunctional biomaterials with combined osteogenic antibacterial properties. offer an alternative traditional grafts, minimizing complications such as multiple surgeries, high antibiotic dosages, lengthy recovery periods. This review examines repair mechanisms infectious microenvironment highlights various foster both anti-infective processes. Emerging design strategies are also discussed provide forward-looking perspective on treating clinically significant outcomes.

Language: Английский

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Jahn–Teller-Driven Electronic Modulation of Bio-Heterojunction for Wound Regeneration after Postoperative Tumor Resection

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Abundant ·OH, 1O2, and ·O2- provide an efficient methodology for rapid tumor bacteria killing, whereas a limitation focuses on the catalytic efficiency. Thus, Jahn-Teller-driven electronic modulation of bioheterojunction (bioHJ) platform is developed remedy in diabetic infectious wound regeneration after postoperative resection. The bioHJ composed MoTe2/MnO2 glucose oxidase (GOx). GOx depletes to H2O2, which intercepts their metabolism. H2O2 can be further converted into highly lethal ·OH owing peroxidase-mimetic activity via Jahn-Teller effect, while GSH consumed due its GPx-mimetic activity. Both amplified upon NIR irradiation as NIR-activatable enzyme-mimetic activities. In vivo studies subcutaneous model authenticate ability kill tumor, defeat bacterial infection, accelerate regeneration. This work enlightens powerful resection using engineered bioHJ.

Language: Английский

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2A-biohydrogels accelerate diabetic wound healing by promoting M2 macrophage polarization and functionalized mitochondrial transfer to endothelial cells

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163130 - 163130

Published: April 1, 2025

Language: Английский

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Overcoming biological inertness: multifaceted strategies to optimize PEEK bioactivity for interdisciplinary clinical applications

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Polyether ether ketone (PEEK), characterized by a comparable elastic modulus to human bone with high wear resistance, radiolucency, and biocompatibility, demonstrates considerable promise for clinical applications.

Language: Английский

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Zinc‐doped bioactive glass‐functionalized polyetheretherketone to enhance the biological response in bone regeneration

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: 112(9), P. 1565 - 1577

Published: March 21, 2024

Abstract Polyether ether ketone (PEEK) is gaining recognition as a highly promising polymer for orthopedic implants, attributed to its exceptional biocompatibility, ease of processing, and radiation resistance. However, long‐term in vivo application faces challenges, primarily due suboptimal osseointegration from postimplantation inflammation immune reactions. Consequently, biofunctionalization PEEK implant surfaces emerges strategic approach enhance increase the overall success rates these implants. In our research, we engineered multifaceted through situ integration chitosan‐coated zinc‐doped bioactive glass nanoparticles (Zn‐BGNs). This novel fabrication imbues with immunomodulatory capabilities while bolstering potential. The biofunctionalized composite elicited several advantageous responses; it facilitated M2 macrophage polarization, curtailed production inflammatory mediators, augmented osteogenic differentiation bone marrow mesenchymal stem cells. experimental findings underscore vital intricate role implants preserving normal immunity metabolism. study posits that utilizing chitosan‐BGNs represents direct effective method creating multifunctional These are designed facilitate biomineralization immunomodulation, making them especially apt applications.

Language: Английский

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Additive manufacturing of degradable metallic scaffolds for material-structure-driven diabetic maxillofacial bone regeneration

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 413 - 426

Published: June 1, 2024

The regeneration of maxillofacial bone defects associated with diabetes mellitus remains challenging due to the occlusal loading and hyperglycemia microenvironment. Herein, we propose a material-structure-driven strategy through additive manufacturing degradable Zn–Mg–Cu gradient scaffolds. in situ alloying Mg Cu endows Zn alloy admirable compressive strength for mechanical support uniform degradation mode preventing localized rupture. scaffolds manifest favorable antibacterial, angiogenic, osteogenic modulation capacity mimicked hyperglycemic microenvironment, promote differentiation early late stages, respectively. In addition, expedite diabetic ingrowth by combining metabolic regulation effect divalent metal cations hyperboloid suitable permeability structure. RNA sequencing further reveals that RAC1 might be involved formation regulating transport uptake glucose related GLUT1 osteoblasts, contributing cell function recovery. Inspired healing structural cues, this study offers an essential understanding designation underlying mechanisms regeneration.

Language: Английский

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GAPDH-Silence Microsphere via Reprogramming Macrophage Metabolism and eradicating Bacteria for Diabetic infection bone regeneration

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 29, 2024

Macrophage metabolism dysregulation, which is exacerbated by persistent stimulation in infectious and inflammatory diseases, such as diabetic bone defects (DIBD), eventually leads to the failure of repair. Here, we have developed an injectable, macrophage-modulated GAPDH-Silence drug delivery system. This microsphere comprises chondroitin sulfate methacrylate (CM) methacrylated gelatin (GM), while dimethyl fumarate (DMF)-loaded liposome (D-lip) encapsulated within (CM@GM), named D-lip/CM@GM. Triggered over-expressed collagenase DIBD, microspheres degrade release D-lip. D-lip could modulate inhibiting GAPDH, suppresses over-activation glycolysis, thus preventing response macrophages vitro. While beneficial for macrophages, D-lip/CM@GM harmful bacteria. crucial glycolysis staphylococcal species (S. aureus), can be effectively countered We are utilizing existing drugs innovative ways target central effective eradication In DIBD model, our results confirmed that enhanced bacteria clearance reprogrammed dysregulated metabolism, thereby significantly improving regeneration. conclusion, this system may provide a viable strategy promote infection

Language: Английский

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Diabetic Microenvironment‐Unlocked BioHJzyme with H₂S Evolution for Robust Anti‐Pathogens and Hyperinflammatory Wound Regeneration Through TGF‐β/Smad Pathway

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(48)

Published: Sept. 30, 2024

Abstract Deferred diabetic skin healing is an ever‐growing complication owing to the hyperglycemic microenvironment, which accelerates generation of advanced glycated end products (AGEs) and provides a hotbed for pathogenic infection. Here, H 2 S‐evolving bio‐heterojunction enzyme (BioHJzyme), consisted by MXene/FeS glucose oxidase (GOx) devised. It presents glutathione peroxidase (GPx)‐ (POD)‐mimetic antibacterial activity anti‐pathogens wound regeneration AGEs depression. The GOx catalyzes glucose, resulting in reducing bacterial nutrient supplying O . POD‐mimetic BioHJzyme hydroxyl radical (•OH) with turnover number 4.45 × 10 −1 s , while GPx‐mimetic it consumes further •OH accumulation. can be enhanced near infrared laser (NIR) irradiation efficient separation electron‐hole pairs originated from heterostructure, NIR‐activatable 1 production. Moreover, evolves S acidic environment, acting as donor, protects cells around oxidative damage AGEs, rescues mitochondrial respiration, improves extracellular matrix deposition ameliorates dysfunction fibroblasts through TGF‐β/Smad pathway. work proof‐of‐concept bacteria‐invaded via BioHJzyme.

Language: Английский

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