Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems DOI Creative Commons
Roland Wohlgemuth

Metabolites, Journal Year: 2023, Volume and Issue: 13(10), P. 1097 - 1097

Published: Oct. 19, 2023

Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis, structural identification, involved in numerous life science milestones. The renewed focus on small molecule domain biological cells has also created an increasing awareness rising gap between metabolites identified prepared as pure compounds. design engineering resource-efficient straightforward synthetic methodologies production diverse metabolite-like compounds attracted much interest. variety metabolic pathways provides a wonderful blueprint designing simplified routes to desired metabolites. Therefore, biocatalytic systems become key enabling tools number can then be utilized standards, enzyme substrates, inhibitors, or other products, discovery novel functions.

Language: Английский

Exploring nanocarriers as innovative materials for advanced drug delivery strategies in onco-immunotherapies DOI
Pratiksha Tiwari, Ravi Prakash Shukla, Krishna Yadav

et al.

Journal of Molecular Graphics and Modelling, Journal Year: 2024, Volume and Issue: 128, P. 108702 - 108702

Published: Jan. 3, 2024

Language: Английский

Citations

20
Etiopathology, Epidemiology, Diagnosis, and Treatment of Fungal Keratitis DOI
Amol Chhatrapati Bisen, Sachin Nashik Sanap, Sristi Agrawal

et al.

ACS Infectious Diseases, Journal Year: 2024, Volume and Issue: 10(7), P. 2356 - 2380

Published: June 7, 2024

Fungal keratitis (FK) is a severe ocular condition resulting from corneal infection that prevalent in tropical countries, particularly developing regions of Asia and Africa. Factors like lens misuse, inappropriate steroid use, diagnostic challenges have provoked the epidemic. FK causes significant vision impairment, scarring, deformities. Accurate pathological diagnosis crucial for effective therapeutic intervention. Topical antifungal therapy with surface healing medications proves preventing fungal-borne ulcers. Managing requires comprehensive understanding fungal pathogenesis, guiding formulation strategies preventive measures to curb global blindness. This review provides in-depth insights into FK, covering etiology, epidemiology, interventions, resistance, limitations, prevention, future perspectives on disease management.

Language: Английский

Citations

9
Ocular Bioanalysis of Moxifloxacin and Ketorolac Tromethamine in Rabbit Lacrimal Matrix Using Liquid Chromatography–Tandem Mass Spectrometry DOI
Amol Chhatrapati Bisen, Rabi Sankar Bhatta

Bioanalysis, Journal Year: 2024, Volume and Issue: 16(4), P. 219 - 232

Published: Jan. 10, 2024

Aim: The fixed-dose combination of moxifloxacin (MOXI) and ketorolac tromethamine (KTR) is widely used for the treatment bacterial keratitis. Thus, a new LC–MS/MS method was developed to determine MOXI KTR in lacrimal fluid. Methods: Bioanalysis performed using Shimadzu 8050 electrospray ionization-positive mode validated per US FDA guidelines. Isocratic separation with Waters Symmetry C18 column methanol 0.1% formic acid containing deionized water (85:15, v/v). Results & conclusion: An easy, quick selective established applied assess ocular pharmacokinetic profile commercially available formulation KTR. Based on data, this work describes pharmacokinetics-based dosage regimen calculations their clinical significance.

Language: Английский

Citations

8
A review on polymers in ocular drug delivery systems DOI Creative Commons
Amol Chhatrapati Bisen, Arpon Biswas, Ayush Dubey

et al.

MedComm – Biomaterials and Applications, Journal Year: 2024, Volume and Issue: 3(2)

Published: April 23, 2024

Abstract Amid the escalating prevalence of eye diseases and intricate nature as a crucial target organ for drug delivery, researchers face significant challenges in developing delivery systems tailored specifically ocular complications. Addressing gaps current conventional system (ODDS) is this can be achieved by incorporating polymers while designing newer ODDS. This review aims to offer concise overview diverse utilized development ODDS, designed address various conditions disorders, enhance treatment outcomes, ensure patient adherence. Introducing anatomy different routes administration, alongside barriers encountered, presents polymer‐based renowned their unique properties facilitating engineering specialized devices enhanced delivery. Further discussions delve into applications ophthalmology. Emphasis placed on emerging technologies available market treating diseases, underscoring potential revolutionizing healthcare. The also addresses translating these advancements clinical practice, highlighting versatility disorders through customizable sustained

Language: Английский

Citations

7
Extracellular vesicles-powered immunotherapy: Unleashing the potential for safer and more effective cancer treatment DOI
Pratiksha Tiwari, Krishna Yadav, Ravi Prakash Shukla

et al.

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 756, P. 110022 - 110022

Published: April 30, 2024

Language: Английский

Citations

7
Ophthalmic nano-bioconjugates: Critical Challenges and Technological Advances DOI
Sachin Nashik Sanap, Amol Chhatrapati Bisen, Sristi Agrawal

et al.

Therapeutic Delivery, Journal Year: 2023, Volume and Issue: 14(7), P. 419 - 441

Published: July 1, 2023

Ophthalmic disease can cause permanent loss of vision and blindness. Easy-to-administer topical systemic treatments are preferred for treating sight-threatening disorders. Typical ocular anatomy makes ophthalmic drug delivery challenging. Various novel nano-drug approaches developed to attain the desired bioavailability in eye by increasing residence time improved permeability across cornea. The review focuses on methods that biocompatible, safe highly therapeutic. Novelty nanocarrier design modification overcome their drawbacks make them potential carriers disorders both anterior posterior segments. This briefly discussed technologies, patented developments, clinical trial data support nanocarriers' use delivery.

Language: Английский

Citations

13
Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study DOI
Amol Chhatrapati Bisen,

Anjali Mishra,

Sristi Agrawal

et al.

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(6)

Published: May 10, 2024

Abstract Managing ocular microbial infections typically requires pharmacotherapy using antibiotic eye drops, such as moxifloxacin hydrochloride (MFX), combined with an antifungal agent like amphotericin B (AB). We carried out and validated LC–MS/MS assay to quantify these compounds in rabbit tear fluid order look into the pharmacokinetics of two drugs. employed a protein precipitation technique for extraction drugs under examination. A Waters Symmetry C 18 column was used separate analytes internal standard. The composition mobile phase (A) 0.1% v/v formic acid water (B) methanol. detection MFX AB accomplished through utilization positive ion electrospray ionization multiple reaction monitoring mode. linearity curves both exhibited acceptable trendline across concentration range 2.34–300 ng/mL 7.81–1000 surrogate fluid. lower limit quantitation 2.34 ng/mL, while AB, it 7.81 ng/mL. approach strictly validated, encompassing tests selectivity, (with r 2 > 0.99), precision, accuracy, matrix effects, stability. Consequently, we this method evaluate profiles following single topical doses.

Language: Английский

Citations

5
Evaluation of dimedone-derived compounds as inhibitors against human colon cancer: Insights from 2D-QSAR, ADMET prediction, Osiris, Molinspiration, and molecular modeling DOI Creative Commons
Khaoula Mkhayar,

Kaouakeb ELKHATTABI,

Rachida Elkhalabi

et al.

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Journal Year: 2023, Volume and Issue: 51(11), P. 100330 - 100330

Published: Oct. 18, 2023

For this research, we performed a 2D-QSAR analysis on set of 34 molecules derived from dimedone with inhibitory activity against human colon cancer (HT-29). The investigation incorporated principal component (PCA), multiple linear regression (MLR), non-linear (MNLR), and artificial neural network (ANN). evaluations the QSAR models demonstrated high predictive power (R2MLR=0.884; R2CV (MLR)=0.86), (R2MNLR=0.810; (MNLR)=0.879), (R2ANN=0.9; R2CV(ANN)=0.89). reliability was validated through internal, external, Y-randomization, validation domain applicability assessments. Utilizing model predictions, designed four new molecular structures that exhibited superior HT-29 cell line compared to previously tested compounds. Subsequently, examined ADMET molinspiration, Osiris properties results revealed excellent predictions molinspiration for all compounds, while only one compound fulfilled properties. Molecular docking employed investigate bindings between newly molecule C c-Met protein. findings indicated stability in c-Met. Finally, MD simulations were assess binding modes C. Based results, shows promise as potential agonist candidate. Overall, our suggest investigated has serve novel inhibitor cancer.

Language: Английский

Citations

10
Prediction of ADMET profile and anti-inflammatory potential of chamuangone DOI Creative Commons
Sompot Jantarawong,

Puripat Wathanaphanit,

Pharkphoom Panichayupakaranant

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 23, 2025

Chamuangone, a compound extracted from the leaves of Garcinia cowa, exhibits various biological activities. Yet, its absorption, distribution, metabolism, excretion, and toxicity (ADMET) profile as well anti-inflammatory effects in macrophages remain unexplored. In this study, we employed computational tool to predict ADMET chamuangone performed molecular docking simulations assess interactions with key proteins involved inflammatory pathways. Additionally, vitro experiments using lipopolysaccharide (LPS)-induced RAW264.7 were evaluate effects. The present findings suggest that has favorable properties, interacts strongly major inflammation, activity LPS-induced macrophages, making it promising agent.

Language: Английский

Citations

0
Comparative Analysis of Blood MMP-9 Concentration in Alcohol- and Opioid-Addicted Patients DOI Creative Commons

Tamar Kartvelishvili,

Nelly Sapojnikova, Nino Asatiani

et al.

Diseases, Journal Year: 2025, Volume and Issue: 13(2), P. 30 - 30

Published: Jan. 24, 2025

Background/Objectives: In brain physiology and disease, MMP-9 is a significant apparently peculiar factor. Numerous studies have implicated neuroinflammatory processes involving in the pathophysiology of addiction. This study aims to evaluate plasma level as biomarker for stages alcohol opioid Methods: The case subjects were patients with quantitative assessment concentration was performed using monoclonal antibodies against human MMP-9. Results: levels dependence differ from concentrations healthy donors. During intoxication stage, individuals are similar higher than control group. While close after withdrawal, it stays increased during withdrawal. When measured three distinct intoxicated states (light, moderate, heavy) cases addiction, results all similar. Two (methadone buprenorphine) withdrawals—following methadone, buprenorphine, heroin abuse—were associated high levels.

Language: Английский

Citations

0