Comprehensive Analysis of Subtypes and Identification of Key lncRNAs Based on Glutamine Metabolism-Related Long Noncoding RNAs DOI Open Access
Yuwei Feng, Xiao Wei Sun,

Tiangu Yang

et al.

Computational and Mathematical Methods in Medicine, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 16

Published: April 29, 2022

Long noncoding RNAs (lncRNAs) are becoming a critical class of metabolic regulate molecule in cancer. Glutamine is regulator that contributes to each the core tasks proliferating tumor cells. Thus, we aimed evaluate association lncRNAs with glutamine metabolism lung adenocarcinoma (LUAD).Using single-sample gene set enrichment analysis (ssGSEA), LUAD specimens were assigned scores based on metabolism-related genes, and shared common three different data cohorts identified. ConsensusClusterPlus was used perform unsupervised clustering patients LUAD. Key identified by first-order partial correlation analysis.A total 11 metabolism-associated cohorts, classified into subtypes expressions related genes. C1 exhibited shorter overall survival (OS), poor genomic instability, inadequate infiltration immune cell types microenvironment (TME) representative immunodeficiency phenotype. C2 represented immunosuppressive phenotype while C3 activation phenotype, exhibiting highest sensitivity immunotherapy. Nine localized nucleus. Finally, key lncRNAs, significantly enriched multiple pathways, screened found be remarkably OS LUAD.We LUAD, which reflected OS, genomic, TME features, may contribute further study cancer metabolism.

Language: Английский

A new ferroptosis-related signature model including messenger RNAs and long non-coding RNAs predicts the prognosis of gastric cancer patients DOI Creative Commons
Yang Liu, Yanqing Liu, Shujun Ye

et al.

Journal of Translational Internal Medicine, Journal Year: 2023, Volume and Issue: 11(2), P. 145 - 155

Published: June 1, 2023

Gastric cancer (GC) is among the most malignant tumor types, which causes heavy healthy and economic burden to people societies all around world. Establishment of an effective set prognostic marker will benefit a lot treatment GC patients clinically. Ferroptosis newly identified regulated cell death modality, with tight relevance development. However, its application in prognosis has not been studied detail. Deregulated messenger RNA (mRNA) long non-coding (lncRNA) expression profile can serve as novel for predicting survival relapse patients.We downloaded ferroptosis-related gene microarray data, clinicopathologic information list 259 genes from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), database, respectively. Then, correlation analysis, univariate multivariate Cox regression analysis were used construct model GC. we validated GEO datasets. Finally, evaluated differences immune microenvironment between high- low-risk groups.We utilized mRNA lncRNA successfully (incorporating 2 mRNAs 15 lncRNAs) Our model, integrating diverse clinical traits critical factors GC, showed desirable efficacy patients. This also manifested effectively validation by using external patients' data.Our study developed signature predict gastric had favorable predictive ability. may greatly boost practice.

Language: Английский

Citations

37
STAT3 signaling in prostate cancer progression and therapy resistance: An oncogenic pathway with diverse functions DOI Creative Commons
Mehrdokht Sadrkhanloo, Mahshid Deldar Abad Paskeh, Mehrdad Hashemi

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 158, P. 114168 - 114168

Published: Jan. 3, 2023

The categorization of cancers demonstrates that prostate cancer is the most common malignancy in men and it causes high death annually. Prostate patients are diagnosed mainly via biomarkers such as PSA test show poor prognosis. cells rapidly diffuse into different parts body their metastasis also a reason for death. Current therapies include chemotherapy, surgery radiotherapy well targeted therapy. progression regulated by factors STAT3 signaling among them. Growth cytokines IL-6 can induce shows carcinogenic impact. Activation occurs promotes malignant behavior tumor cells. Induction increases glycolysis proliferation prevents apoptosis. Furthermore, induces EMT mechanism increasing metastasis. stimulates drug resistance limitation current works lack experiment related to role radio-resistance tumor. Calcitriol, capsazepine β-elemonic compounds capable targeting its inhibition In addition natural products, small molecules have been developed

Language: Английский

Citations

30
Long noncoding RNA MAGI2-AS3 regulates the H2O2 level and cell senescence via HSPA8 DOI Creative Commons
Yingmin Zhang, Xinhua Qiao, Lihui Liu

et al.

Redox Biology, Journal Year: 2022, Volume and Issue: 54, P. 102383 - 102383

Published: June 30, 2022

The redox homeostasis system regulates many biological processes, intracellular antioxidant production and signaling. However, long noncoding RNAs (lncRNAs) involved in regulation have rarely been reported. Herein, we reported that downregulation of MAGI2-AS3 decreased the superoxide level Human fibroblasts (Fbs), a replicative aging model, as detected by fluorescent probes dihydroethidium (DHE) MitoSOX™ Red. RNA pulldown combined with mass spectrometry showed HSPA8 is novel interacting protein MAGI2-AS3, which was further confirmed photoactivatable ribonucleoside-enhanced crosslinking immunoprecipitation (PAR-CLIP). Downregulation hydrogen peroxide (H2O2) content stabilizing via inhibiting protesome degradation HSPA8. Further evidence interacted C-terminal domain (CTD) delayed cell senescence, while this antiaging effect abolished knockdown. underlying molecular mechanism knockdown inhibited senescence mediated suppression ROS/MAP2K6/p38 signaling pathway. Taken together, these findings revealed lncRNA H2O2 level, identifying potential application.

Language: Английский

Citations

27
lncRNA-mediated ceRNA network in bladder cancer DOI Creative Commons
Kun Li,

Tongyue Yao,

Ziqiang Wang

et al.

Non-coding RNA Research, Journal Year: 2022, Volume and Issue: 8(2), P. 135 - 145

Published: Dec. 14, 2022

Bladder cancer is a common disease associated with high rates of morbidity and mortality. Although immunotherapy approaches such as adoptive T-cell therapy immune checkpoint blockade have been investigated for the treatment bladder cancer, their off-target effects ability to affect only single targets led clinical outcomes that are far from satisfactory. Therefore, it important identify novel can effectively control tumor growth metastasis. It well known long noncoding RNAs (lncRNAs) powerful regulators gene expression. Increasing evidence has shown dysregulated lncRNAs in involved cell proliferation, migration, invasion, apoptosis, epithelial-mesenchymal transition (EMT). In this review, we focus on roles underlying mechanisms lncRNA-mediated competing endogenous RNA (ceRNA) networks regulation progression. addition, discuss potential targeting ceRNA overcome resistance its association clinicopathological features patients. We hope review will stimulate research develop more effective therapeutic treatment.

Language: Английский

Citations

24
Urine biomarkers for Alzheimer's disease: A new opportunity for wastewater-based epidemiology? DOI Creative Commons
Arnoldo Armenta-Castro,

Mónica T. Núñez-Soto,

Kassandra O. Rodríguez-Aguillón

et al.

Environment International, Journal Year: 2024, Volume and Issue: 184, P. 108462 - 108462

Published: Jan. 28, 2024

While Alzheimer's disease (AD) diagnosis, management, and care have become priorities for healthcare providers researcher's worldwide due to rapid population aging, epidemiologic surveillance efforts are currently limited by costly, invasive diagnostic procedures, particularly in low middle income countries (LMIC). In recent years, wastewater-based epidemiology (WBE) has emerged as a promising tool public health assessment through detection quantification of specific biomarkers wastewater, but applications non-infectious diseases such AD remain limited. This early review seeks summarize AD-related urine other peripheral biofluids discuss their potential integration WBE platforms guide the first prospective field. Promising results been reported clinical settings, indicating amyloid β, tau, neural thread protein, long non-coding RNAs, oxidative stress markers dysregulated metabolites questions regarding concentration stability wastewater correlation between levels sewage circulation must be addressed future studies before comprehensive systems can developed.

Language: Английский

Citations

6
The DNA damage repair-related lncRNAs signature predicts the prognosis and immunotherapy response in gastric cancer DOI Creative Commons

Zidan Zhao,

Tsz Kin Mak,

Yuntao Shi

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 29, 2023

Background Gastric cancer (GC) is one of the most prevalent cancers, and it has unsatisfactory overall treatment outcomes. DNA damage repair (DDR) a complicated process for signal transduction that causes cancer. lncRNAs can influence formation incidence cancers by influencing DDR-related mRNAs/miRNAs. A lncRNA prognostic model urgently needed to improve strategies. Methods The data GC samples were obtained from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) datasets. total 588 mRNAs involved in DDR selected MSigDB, 62 differentially expressed TCGA-STAD obtained, 137 correlated with these mRNAs. Univariate Cox regression least absolute shrinkage selection operator (LASSO) analyses used develop model. Based on risk model, gene signature A/B low-risk high-risk groups was identified further validation. Results prognosis 5 genes (AC145285.6, MAGI2-AS3, AL590705.3, AC007405.3, LINC00106) constructed classified into two groups. We found patients score had better OS than those score. group tended have higher TME scores. also proportion resting CD4 T cells, monocytes, M2 macrophages, dendritic mast whereas subgroup greater abundance activated follicular helper M0 M1 macrophages. observed significant differences T-cell exclusion score, dysfunction, MSI, TMB between In addition, we treated immunotherapy low-RS longer survival high-RS group. Conclusion role TME, clinicopathological characteristics, prognosis, drug sensitivity. discovered prognosis. This work provides foundation improving response among GC.

Language: Английский

Citations

10
The emerging landscape of small nucleolar RNA host gene 10 in cancer mechanistic insights and clinical relevance DOI
Jingyu Zhu, Zhongyu Jian,

Fangteng Liu

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: 127, P. 111590 - 111590

Published: Jan. 10, 2025

Language: Английский

Citations

0
Activation of JNK/p38 MAPK Signaling Pathway by lncRNA DGCR5 Promotes Nucleus Pulposus Cell Degeneration and Pyroptosis in Intervertebral Disk Degeneration DOI
Hua Zhong,

Lebin Guo,

Wei Guo

et al.

Neurosurgery, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Currently, the role of long noncoding RNA (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) in intervertebral disk degeneration (IDD) remains unclear. This study explored molecular mechanisms how lncRNA DGCR5 promoted human nucleus pulposus cells (NPCs) and pyroptosis during IDD. NPCs were identified by microscopic observation, flow cytometry, immunofluorescence. An vitro NPC model was induced using lipopolysaccharides treatment. SP600125 SB203580 used to inhibit c-Jun N-terminal kinase (JNK) p38 MAPK signaling, respectively. Quantitative real-time polymerase chain reaction or Western blot performed detect DGCR5, JNK, phospho-JNK, MAPK, phospho-p38 expressions tissues NPCs. Cell Counting Kit-8 assay conducted activity. expression levels extracellular matrix-associated proteins (including collagen II, aggrecan, matrix metalloproteinase 3 [MMP3]) pyroptosis-associated nucleotide oligomerization domain-like receptors family pyrin domain containing 3), cleaved caspase-1, lactate dehydrogenase, fragment Gasdermin D (GSDMD) Enzyme-linked immunosorbent interleukin (IL)-1beta (IL-1β) IL-18 supernatants. upregulated, JNK/p38 signaling activated both IDD patients lipopolysaccharides-induced Inhibition enhanced proliferation, II aggrecan expression, while inhibited MMP expression. Silencing suppressed activity, reduced but Similar findings also observed for 3, GSDMD, IL-1β, as well released dehydrogenase level. However, overexpression yielded opposite results. These data suggest that regulates promote through pathway.

Language: Английский

Citations

0
In vitro investigation of epigenetic regulators related to chemo-resistance and stemness of CD133+VE cells sorted from U87MG cell line DOI Creative Commons
Elham Abusharieh, Nazneen Aslam,

Malek Zihlif

et al.

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149432 - 149432

Published: March 1, 2025

Language: Английский

Citations

0
LncRNA MAGI2-AS3 Inhibits Prostate Cancer Progression by Targeting the miR-142-3p DOI
Renbao Hu, Pei Wu, Jianhui Liu

et al.

Hormone and Metabolic Research, Journal Year: 2022, Volume and Issue: 54(11), P. 754 - 759

Published: Aug. 9, 2022

Abstract Prostate cancer is a common male with high morbidity and mortality worldwide. According to current research, the integration of long non-coding RNA (lncRNAs) microRNA(miRNAs) can be expressed in variety cancers play an important role diagnosis. Based on this, this study explored clinical lncRNA MAGI2-AS3 (MAGI2-AS3) prostate cancer. By detecting expression levels miR-142-3p, correlation between characteristics data was analyzed. ROC curve analysis performed area under (AUC) used evaluate diagnostic value distinguishing patients from healthy controls. The function cells through CCK-8 Transwell assays, relationship miR-142-3p investigated by luciferase activity assay. has descended while ascendant one serum samples cells. revealed that AUC 0.953 for MAGI2-AS3, sensitivity 91.5% specificity 84.7%. Overexpression LNCaP PC3 suppressed biological cell including proliferation capacity, migration level, invasion. considered biomarker inhibited progression targeting miR-142-3p.

Language: Английский

Citations

15