Journal of Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 74(2)
Published: March 27, 2024
Language: Английский
Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)
Published: May 31, 2024
Abstract The immune response holds a pivotal role in cardiovascular disease development. As multifunctional cells of the innate system, macrophages play an essential initial inflammatory that occurs following injury, thereby inducing subsequent damage while also facilitating recovery. Meanwhile, diverse phenotypes and phenotypic alterations strongly associate with distinct types severity diseases, including coronary heart disease, valvular myocarditis, cardiomyopathy, failure, atherosclerosis aneurysm, which underscores importance investigating macrophage regulatory mechanisms within context specific diseases. Besides, recent strides single-cell sequencing technologies have revealed heterogeneity, cell–cell interactions, downstream therapeutic targets at higher resolution, brings new perspectives into macrophage-mediated potential Remarkably, myocardial fibrosis, prevalent characteristic most cardiac remains formidable clinical challenge, necessitating profound investigation impact on fibrosis In this review, we systematically summarize functional plasticity diseases unprecedented insights introduced by technologies, focus different causes characteristics especially relationship between inflammation (myocardial infarction, pressure overload, dilated diabetic cardiomyopathy aging) vascular injury (atherosclerosis aneurysm). Finally, highlight preclinical/clinical targeting strategies translational implications.
Language: Английский
Citations
53
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: Jan. 23, 2025
Language: Английский
Citations
3
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2553 - 2571
Published: March 1, 2024
Purpose: Accumulating evidence indicates that mesenchymal stem cells (MSCs)-derived exosomes hold significant potential for the treatment of atherosclerosis. However, large-scale production and organ-specific targeting are still challenges further clinical applications. This study aims to explore targeted efficiency therapeutic biomimetic platelet membrane-coated exosome-mimetic nanovesicles (P-ENVs) in Methods: To produce (ENVs), MSCs were successively extruded through polycarbonate porous membranes. P-ENVs engineered by fusing MSC-derived ENVs with membranes characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), Western blot. The stability safety also assessed. efficacy was evaluated an vivo imaging system (IVIS) spectrum immunofluorescence. Histological analyses, Oil Red O (ORO) staining, blot used investigate anti-atherosclerotic effectiveness P-ENVs. Results: Both exhibited similar characteristics exosomes. Subsequent miRNA sequencing revealed their mitigate atherosclerosis influencing biological processes related cholesterol metabolism. In ApoE −/− mice model, intravenous administration enhanced atherosclerotic plaques, resulting a reduction lipid deposition necrotic core area. Our vitro experiments showed promoted efflux reduced total content foam cells. Further attenuated intracellular accumulation upregulating expression critical transporters ABCA1 ABCG1. Conclusion: highlighted as novel nano-drug delivery platform enhancing drug while concurrently mitigating adverse reactions therapy. Keywords: nanovesicles, biomimicry, delivery,
Language: Английский
Citations
9
Cell Transplantation, Journal Year: 2025, Volume and Issue: 34
Published: Jan. 1, 2025
Mesenchymal stem cells (MSCs) are pluripotent with self-renewal. They play a critical role in cell therapy due to their powerful immunomodulatory and regenerative effects. Recent studies suggest that one of the key therapeutic mechanisms MSCs seems derive from paracrine product, called extracellular vesicles (EVs). The EVs contain much DNA, messenger RNA (mRNA), microRNA, protein components, which can exert intracellular communication target cells. In clinical applications, MSC-EVs have been widely used tissue repair immune disorder diseases. However, there serval issues need be considered such as how accomplish large-scale production verify exact mechanism EVs. this review, we summarize current progress discuss challenges future MSC-EVs.
Language: Английский
Citations
1
Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: unknown, P. 103517 - 103517
Published: April 1, 2025
Language: Английский
Citations
1
Molecules, Journal Year: 2023, Volume and Issue: 28(15), P. 5904 - 5904
Published: Aug. 5, 2023
Tectorigenin is a well-known natural flavonoid aglycone and an active component that exists in numerous plants. Growing evidence suggests tectorigenin has multiple pharmacological effects, such as anticancer, antidiabetic, hepatoprotective, anti-inflammatory, antioxidative, antimicrobial, cardioprotective, neuroprotective. These properties provide the basis for treatment of many kinds illnesses, including several types cancer, diabetes, hepatic fibrosis, osteoarthritis, Alzheimer’s disease, etc. The purpose this paper to comprehensive summary review sources, extraction synthesis, toxicity, pharmacokinetics, delivery strategy aspects tectorigenin. may exert certain cytotoxicity, which related administration time concentration. Pharmacokinetic studies have demonstrated main metabolic pathways rats are glucuronidation, sulfation, demethylation methoxylation, but it exhibits poor bioavailability. From our perspective, further research on should cover: exploring targets mechanisms action; finding appropriate concentration balance effects toxicity; attempting diversified strategies improve bioavailability; structural modification obtain derivatives with higher activity.
Language: Английский
Citations
18
Cells, Journal Year: 2024, Volume and Issue: 13(10), P. 855 - 855
Published: May 17, 2024
Cardiovascular diseases continue to challenge global health, demanding innovative therapeutic solutions. This review delves into the transformative role of mesenchymal stem cells (MSCs) in advancing cardiovascular therapeutics. Beginning with a historical perspective, we trace development cell research related diseases, highlighting foundational approaches and evolution cell-based treatments. Recognizing inherent challenges MSC-based therapeutics, which range from understanding pro-reparative activity MSCs tailoring patient-specific treatments, emphasize need refine pro-regenerative capacity these cells. Crucially, our focus then shifts strategies fourth generation therapies: leveraging secretomic prowess MSCs, particularly extracellular vesicles; integrating biocompatible scaffolds artificial sheets amplify MSCs’ potential; adopting three-dimensional ex vivo propagation tailored specific tissue niches; harnessing promise genetic modifications for targeted repair; institutionalizing good manufacturing practice protocols ensure safety efficacy. We conclude reflections on advancements, envisaging future landscape redefined by regeneration. offers both consolidation current view toward imminent horizons.
Language: Английский
Citations
6
Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)
Published: Aug. 30, 2023
Abstract In the last decade, human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM)-based cell therapy has drawn broad attention as a potential for treating injured hearts. However, mass production of hiPSC-CMs remains challenging, limiting their translational in regenerative medicine. Therefore, multiple strategies including cycle regulators, small molecules, co-culture systems, and epigenetic modifiers have been used to improve proliferation hiPSC-CMs. On other hand, immaturity these proliferative could lead lethal arrhythmias due limited ability functionally couple with resident cardiomyocytes. To achieve functional maturity, numerous methods such prolonged culture, biochemical or biophysical stimulation, vivo transplantation, 3D culture approaches employed. this review, we summarize recent promote hiPSC-CM proliferation, thoroughly review advances promoting maturation, which will serve foundation large-scale mature future clinical applications.
Language: Английский
Citations
14
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3778 - 3778
Published: March 28, 2024
Ischemic heart disease, which is one of the top killers worldwide, encompasses a series problems stemming from compromised coronary blood supply to myocardium. The severity disease ranges an unstable manifestation ischemic symptoms, such as angina, myocardial death, that is, immediate life-threatening condition infarction. Even though patients may survive infarction, resulting ischemia-reperfusion injury triggers cascade inflammatory reactions and oxidative stress poses significant threat function following successful revascularization. Moreover, despite evidence suggesting presence cardiac stem cells, fact cardiomyocytes are terminally differentiated cannot significantly regenerate after accounts for subsequent progression cardiomyopathy failure, current advancements in medicine. In last two decades, researchers have realized possibility utilizing cell plasticity therapeutic purposes. Indeed, cells different origin, bone-marrow- adipose-derived mesenchymal circulation-derived progenitor induced pluripotent all been shown play roles disease. addition, discovery stem-cell-associated paracrine effects has triggered intense investigations into actions exosomes. Notwithstanding seemingly promising outcomes both experimental clinical studies regarding use against positive results fraud or false data interpretation need be taken consideration. review aimed at overviewing application categories including relevant outcomes, well proposed mechanisms underpinning observations.
Language: Английский
Citations
5
Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: 20(7), P. 1671 - 1691
Published: July 18, 2024
Language: Английский
Citations
5