Causal Relationship Between Systemic Circulatory Inflammatory Regulators and Intervertebral Disc Degeneration: A Bidirectional Two-Sample Mendelian Randomization Study DOI Creative Commons
Zixuan Chen, Bo Xu,

Ze-Ling Huang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

Abstract Background In the context of development Intervertebral Disc Degeneration (IDD), inflammatory mediators play a pivotal role. Nevertheless, due to influence microenvironment, causal relationship between specific and IDD remains uncertain. Methods We utilized genetic data concerning systemic circulating regulators obtained from Genome-Wide Association Study (GWAS) analyzing 41 serum cytokines in cohort 8,293 individuals Finland. The for was derived most recent summary statistics conducted within FinnGen consortium, encompassing 37,636 cases 270,964 controls. Our analysis employed bidirectional two-sample Mendelian randomization (MR) techniques, which included several MR methods such as Egger, weighted median, inverse variance (IVW), mode, simple mode. Additionally, MR-PRESSO method identify horizontal pleiotropy, heterogeneity quantified using Cochran Q statistic, Egger intercept performed assess pleiotropy. Results established relationships three factors IDD. Elevated levels MIP-1β [OR = 0.956, 95% CI: -0.08 -0.006; P 0.02] IFN-G 0.915, -0.16 -0.02; 0.01] expression were associated with reduced risk Conversely, susceptibility linked decrease IL13 0.967, -0.063 -0.004; 0.03]. Conclusion this study, we have identified that exhibit onset progression IDD, supported by predictions.

Language: Английский

The Sanbi Decoction alleviates intervertebral disc degeneration in rats through intestinal flora and serum metabolic homeostasis modulation DOI
Nan Wang, Shuang Chen,

Yimin Xie

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 127, P. 155480 - 155480

Published: Feb. 24, 2024

Language: Английский

Citations

8
Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression DOI Creative Commons

Chia‐Kung Yen,

Hongbo Pan, Hsin‐I Chang

et al.

JOR Spine, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 20, 2025

ABSTRACT Background Resistin may connect obesity and intervertebral disc (IVD) degeneration (IDD) is linked with chronic inflammation. Furthermore, human IDD characterized by high expression of interleukin‐20 (IL‐20). The response nucleus pulposus (NP) cells to tensile forces depends on both the duration magnitude force applied. Nonetheless, interactions among resistin, IL‐20, in regulating function IVD NP remain yet be fully understood. Nuclear factor erythroid 2‐related 2 (NRF2) has been identified as a pleiotropic protein that enhances cellular resistance stress stimuli inflammatory challenges. role NRF2 not well defined, whether influences levels known. Aims This study investigated subjected low‐frequency cyclic stretch stimulation, underlying mechanisms involved. Materials Methods Human were cultured chambers, serum‐starved for 12 h, then 5% strain at 0.1 Hz bioreactor. Control chambers had no stretching. Cells lysed analysis post‐loading. Results stimulation induced IL‐20 dose‐ time‐dependent manner. activation p38 mitogen‐activated kinase, Akt signaling pathways, production toll‐like receptor 4, are necessary render resistin cause release IL‐20. In cells, transcription enzyme‐linked immunosorbent assays revealed led an increase nuclear (NF)‐κB‐DNA binding activities. Exposure inhibited this resistin‐induced NF‐κB expression. Discussion These findings elucidate molecular through which induces also demonstrate can protect against induction.

Language: Английский

Citations

1
Molecular structure of Fgfbp1 protein and its regulation of zebrafish cartilage development and homeostasis: Implications for Wnt signaling and ECM stability DOI
Qiong Wang, Ding Li, Yuming Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142340 - 142340

Published: March 1, 2025

Language: Английский

Citations

1
Causal associations between gut microbiota with intervertebral disk degeneration, low back pain, and sciatica: a Mendelian randomization study DOI

Miaojie Fang,

Wei Liu, Zhan Wang

et al.

European Spine Journal, Journal Year: 2024, Volume and Issue: 33(4), P. 1424 - 1439

Published: Jan. 29, 2024

Language: Английский

Citations

5
Spotlight on necroptosis: Role in pathogenesis and therapeutic potential of intervertebral disc degeneration DOI

Rui Ran,

Shun-Bai Zhang,

Yong-Qiang Shi

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 138, P. 112616 - 112616

Published: July 2, 2024

Language: Английский

Citations

5
Dysregulated lipid metabolism and intervertebral disc degeneration: the important role of ox-LDL/LOX-1 in endplate chondrocyte senescence and calcification DOI Creative Commons

Tan Bing,

Xiang Shanlin,

Jisheng Wang

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Aug. 9, 2024

Lipid metabolism disorders are associated with degeneration of multiple tissues and organs, but the mechanism crosstalk between lipid disorder intervertebral disc (IDD) has not been fully elucidated. In this study we aim to investigate regulatory abnormal signal on endplate chondrocyte (EPC) senescence calcification.

Language: Английский

Citations

5
Lipid metabolic disorders and their impact on cartilage endplate and nucleus pulposus function in intervertebral disk degeneration DOI Creative Commons

Ruixia Wu,

Xia Zhao, Yi Du

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: March 10, 2025

Lipid metabolism encompasses the processes of digestion, absorption, synthesis, and degradation fats within biological systems, playing a crucial role in sustaining normal physiological functions. Disorders lipid metabolism, characterized by abnormal blood levels dysregulated fatty acid have emerged as significant contributors to intervertebral disk degeneration (IDD). The pathogenesis IDD is multifaceted, encompassing genetic predispositions, nutritional metabolic factors, mechanical stressors, trauma, inflammatory responses, which collectively facilitate progression IDD. Although precise mechanisms underlying remain incompletely elucidated, there substantial consensus regarding close association between disorders its development. Intervertebral disks are essential for maintaining spinal alignment. Their primary functions encompass shock preservation curvature, facilitation movement, provision stability. elasticity thickness these effectively absorb daily impacts, safeguard spine, uphold natural curvature flexibility, while also creating space nerve roots prevent compression ensure transmission signals. Research indicates that such disturbances may compromise functionality cartilaginous endplates (CEP) nucleus pulposus (NP), thereby facilitating IDD’s onset progression. CEP integral internal material exchange absorption mitigating NP herniation under load conditions. As central component disks, height providing shock-absorbing capabilities; thus, damage critical structures accelerates Furthermore, contribute through including activation endoplasmic reticulum stress pathways, enhancement oxidative levels, induction cellular pyroptosis alongside inhibition autophagy processes—coupled with promotion inflammation-induced fibrosis fibroblast proliferation leading calcification disks. This review delineates intricate interplay IDD; it anticipated advancing our understanding this will pave way more effective preventive measures therapeutic strategies against future research.

Language: Английский

Citations

0
Guiqi Huoxue Capsule Alleviates Cervical Spondylosis in Rats: Insights from 16S rRNA Sequencing, Lipidomics, and Network Pharmacology DOI

Hongguang Tang,

Wenting Ni,

Dejun Niu

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: 347, P. 119752 - 119752

Published: April 7, 2025

Language: Английский

Citations

0
Identification and validation of endoplasmic reticulum stress-related genes in patients with steroid-induced osteonecrosis of the femoral head DOI Creative Commons
Tingyu Wu, Weipeng Shi,

Yinxue Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 16, 2024

Steroid-induced osteonecrosis of the femoral head (SONFH) is a debilitating condition caused by long-term corticosteroid use, leading to impaired blood flow and bone cell death. The disruption cellular processes promotion apoptosis endoplasmic reticulum stress (ERS) implicated in pathogenesis SONFH. We identified ERS-associated genes SONFH investigated their potential as therapeutic targets. analysed GSE123568 GEO dataset identify differentially expressed (DEGs) related ERS conducted Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses, hub protein-protein interaction (PPI) evaluated functions gene set analysis (GSEA). constructed mRNA-miRNA networks, therapeutics, assessed immune infiltration. performed cross-validation using GSE74089, qRT-PCR on clinical samples from patients with controls, receiver operating characteristic (ROC) curve assess diagnostic performance genes. 195 ERS-related SONFH, which were primarily involved oxidative stress, responses, metabolic pathways. PPI network suggested CXCL8, STAT3, IL1B, TLR4, PTGS2, TLR2, CASP1, CYBB, CAT, HOMX1 be key genes, shown GSEA biological pathways metabolism, modulation, integrity. also 261 microRNAs (miRNAs) well drugs such dibenziodolium N-acetyl-L-cysteine that modulated inflammatory responses Twenty-two subtypes showed significant correlations, positive correlation between activated mast cells Tregs, had fewer dendritic than controls. CYBB TLR4 correlations M1 macrophages CD8 T cells, respectively. Cross-validation confirmed upregulation CASP1 validating bioinformatics findings. An ROC top 10 show promise biomarkers for discovered miRNAs, including hsa-miR-23, influence these therapeutics simvastatin. Immune profiling indicated altered among types. Validation TLR2 potential. findings suggest markers targets

Language: Английский

Citations

3
Visualizing the bibliometrics of the inflammatory mechanisms in intervertebral disc degeneration DOI Creative Commons
Nan Wang,

Weihao Rong,

Yimin Xie

et al.

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 188, P. 112380 - 112380

Published: Feb. 22, 2024

Intervertebral disc degeneration (IVDD) constitutes a crucial pathological foundation for spinal degenerative diseases (SDD) and stands as primary contributor to both low back pain (LBP) disability. The progression of IVDD is linked structural functional alterations in tissues, where an imbalance the inflammatory microenvironment can induce extracellular matrix (ECM) degradation, senescence, apoptosis. This key pathomechanism disease's development, gaining considerable attention recent years. study aims conduct bibliometric analysis publications pertaining mechanisms quantitatively assess current research hotspots directions.

Language: Английский

Citations

2