Aptamer-enabled electrochemical bioplatform utilizing surface-modified g-C3N4/MoS2/PANI nanocomposite for detection of CA125 biomarker

Sensing and Bio-Sensing Research, Journal Year: 2024, Volume and Issue: 45, P. 100669 - 100669

Published: June 28, 2024

Detecting ovarian cancer at an early stage is crucial for enhancing patient outcomes, underscoring the demand efficient and non-invasive detection method. Cancer antigen 125 (CA125) a vital biomarker in detection, there pressing to develop quick, sensitive, simple Nanobiosensors are increasingly being used by scientists due their high selectivity sensitivity, allowing swift precise of wide range biomarkers. This study aimed design fabricate electrochemical nanobiosensor that could accurately selectively detect CA125. The employed graphitic carbon nitrides, molybdenum disulfide, polyaniline (g-C3N4/MoS2/PANI) stabilize aptamer strands on modified glassy electrode. aptasensor was perform labeled CA125, utilizing methylene blue label-free ferrocyanide methods. Ferrocyanide limits were determined be 0.196 U.mL−1 blue, with linear 2–10 detection. results showed electrode exhibited towards CA125 superior stability compared other biomolecules. also displayed impressive performance when analyzing serum patients healthy people. These findings hold significant promise future investigation diagnosis. novel may aid management cancer, ultimately leading better outcomes.

Language: Английский

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Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 746 - 746

Published: Feb. 6, 2025

Homogeneous aptasensors that eliminate the need for probe labeling or immobilization hold significant potential rapid detection of tumor biomarkers. Herein, a homogeneous aptasensor with electrochemical (EC) and electrochemiluminescence (ECL) dual channels was developed by integrating nanochannel-based enrichment DNase I cleavage selective biomarker, carbohydrate antigen 125 (CA125). A two-dimensional (2D) composite prepared assembling CA125-specific aptamer cationic tris(2,2′-bipyridyl)Ru(II) (Ru(bpy)32+), which exhibited both EC ECL properties, onto graphene oxide (GO) nanosheets (Ru(bpy)32+/Apt@GO). vertically ordered mesoporous silica film (VMSF) ultrasmall, uniform, aligned nanochannel arrays rapidly grown on inexpensive disposable indium tin (ITO) electrode, forming interface. Due to size exclusion effect ultrasmall nanochannels in VMSF, Ru(bpy)32+/Apt@GO unable penetrate nanochannels, resulting no detectable Ru(bpy)32+ signal electrode. Upon specific recognition CA125 aptamer, an aptamer-CA125 complex formed subsequently detached from GO. then cleaved complex, releasing allowing dissociate into solution. This enzymatic enabled re-enter binding cycle, amplifying release The electrostatic adsorption VMSF significantly enhanced signals. constructed linear range 0.1 U/mL 100 ng/mL, limit (LOD) 91 mU/mL. For detection, detected over 0.001 U/mL, LOD as low 0.4 demonstrated excellent selectivity successfully applied dual-mode EC/ECL fetal bovine serum samples.

Language: Английский

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Photonic crystal based biosensors: Emerging inverse opals for biomarker detection

Talanta, Journal Year: 2020, Volume and Issue: 221, P. 121615 - 121615

Published: Sept. 2, 2020

Language: Английский

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Label-Free Electrochemical Immunosensor Based on One-Step Electrochemical Deposition of AuNP-RGO Nanocomposites for Detection of Endometriosis Marker CA 125

ACS Applied Bio Materials, Journal Year: 2020, Volume and Issue: 3(11), P. 7620 - 7630

Published: Sept. 28, 2020

Endometriosis is the third most prominent gynecological disorder. Cancer antigen 125 (CA 125) primary serum marker used for late-stage endometriosis diagnosis and management. Herein, we developed a label-free immunosensor electrochemical detection of CA blood samples. The sensor was fabricated by one-step deposition highly conductive gold nanoparticles (AuNPs) reduced graphene oxide (RGO) nanocomposite, via deposition. This method involved in situ reduction HAuCl3 increased electrocatalytic performance. Different analytical techniques confirmed morphology structure AuNP/RGO nanocomposite. In addition, antibody (Ab) immobilized on modified electrode surface through self-assembly monolayer. square wave voltammetry has been utilized to measure interaction Ab (Ag). as-fabricated demonstrates dynamic linear range 0.0001 → 300 U mL-1 lower limit 0.000042 toward CA125 detection. provides acceptable stability, high selectivity, reproducibility. proposed applied patient samples, results confirm reliability that further associated with standard ELISA analysis. AuNP/RGO-based can be as an excellent tool future prospective clinical diagnostics applications.

Language: Английский

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Recent advances on immunosensors for mycotoxins in foods and other commodities

TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 136, P. 116193 - 116193

Published: Jan. 19, 2021

Language: Английский

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A novel electrochemical kidney injury molecule-1 (KIM-1) immunosensor based covalent organic frameworks-gold nanoparticles composite and porous NiCo2S4@CeO2 microspheres: The monitoring of acute kidney injury

Applied Surface Science, Journal Year: 2021, Volume and Issue: 578, P. 152093 - 152093

Published: Nov. 30, 2021

Language: Английский

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Voltammetric biosensors for analytical detection of cardiac troponin biomarkers in acute myocardial infarction

TrAC Trends in Analytical Chemistry, Journal Year: 2020, Volume and Issue: 134, P. 116123 - 116123

Published: Nov. 20, 2020

Language: Английский

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Versatile carbon nanoplatforms for cancer treatment and diagnosis: strategies, applications and future perspectives

Theranostics, Journal Year: 2022, Volume and Issue: 12(5), P. 2290 - 2321

Published: Jan. 1, 2022

Despite the encouraging breakthroughs in medical development, cancer remains one of principle causes death and threatens human health around world.Conventional treatment strategies often kill cells at expense serious adverse effects or great pain, which yet is not able to achieve an effective cure.Therefore, it urgent seek for other novel anticancer approaches improve survival rate life quality patients.During past decades, nanotechnology has made tremendous progress therapy due many advantages such as targeted drug delivery, decreased dosage-related prolonged circulation time.In context nanomedicine, carbon nanomaterials occupy very significant positions.Owing their innate outstanding optical, thermal, electronic, mechanic features, easy functionalization possibility large surface loading, serve only carriers, but also multifunctional platforms combine with diverse diagnosis modalities against cancer.Therefore, developing more carbon-based nanoplatforms plays a critical role theranostics update overview that summarizes recent achievement nanomaterial-mediated theranostic necessity.In this review, five typical widely investigated including graphene, graphdiyne, fullerene, nanotubes quantum dots are introduced detail from aspect based on both tumor microenvironment-involved therapeutic targets.Meanwhile, modern diagnostic methods clinical translatability will be highlighted well.

Language: Английский

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Label-free electrochemical microfluidic biosensors: futuristic point-of-care analytical devices for monitoring diseases

Microchimica Acta, Journal Year: 2022, Volume and Issue: 189(7)

Published: June 10, 2022

Language: Английский

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Simultaneous Detection of Ovarian Cancer-Concerned HE4 and CA125 Markers Based on Cu Single-Atom-Triggered CdS QDs and Eu MOF@Isoluminol ECL

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(10), P. 4795 - 4802

Published: March 3, 2023

Simultaneous detection of different disease markers is significant for clinical diagnosis. In this work, a dual-signal electrochemiluminescence (ECL) immunosensor was constructed the simultaneous carbohydrate antigen 125 (CA125) and human epithelial protein 4 (HE4) ovarian cancer. The results showed that Eu metal–organic framework-loaded isoluminol–Au nanoparticles (Eu MOF@Isolu–Au NPs) could generate strong anodic ECL signal through synergistic interaction; as cathodic luminophore, composite carboxyl-functionalized CdS quantum dots N-doped porous carbon-anchored Cu single-atom catalyst catalyze H2O2 co-reactant to produce large amount •OH O2•–, making signals significantly increase become stable. Based on enhancement strategy, sandwich cancer-associated CA125 HE4 by combining antigen–antibody specific recognition magnetic separation technique. resulting displayed high sensitivity, wide linear response range 0.005∼500 ng mL–1, low limits 0.37 1.58 pg mL–1 HE4, respectively. Furthermore, it had excellent selectivity, stability, practicability in real serum samples. This work establishes framework in-depth design application catalysis sensing.

Language: Английский

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