Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(3), P. 1284 - 1292

Published: Jan. 9, 2024

In this work, a novel nanozyme (Cu@Zr) with all-in-one dual enzyme and fluorescence properties is designed by simple self-assembly. A cascade sensor disodium phenyl phosphate (PPDS) as substrate was first established exploiting the enzymatic activities of phosphatase laccase. Specifically, cleaves P–O bond PPDS to produce colorless phenol, which then oxidized laccase complexed chromogenic agent 4-aminoantipyrine (4-AP) red quinoneimine (QI). Strikingly, NH3 produced urease hydrolysis urea can interact Cu@Zr, accelerating electron transfer rate ultimately leading significantly improved performance reaction. Moreover, at 440 nm Cu@Zr further quenched inner filter effect (IFE) QI. Thus, colorimetric dual-mode strategy for sensitive analysis LODs 3.56 1.83 U/L proposed sensor. Notably, portable swab loaded also prepared in situ detection aid smartphone RGB readout. It provides potentially viable analytical avenue environmental biological analysis.

Language: Английский

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Kinetics Difference-driven Organophosphorus Hydrolase-like Nanozyme-Coded Pattern for Identifying p-Nitrophenyl Pesticides

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Organophosphorus pesticides (OPs) are widely used in agricultural production, posing a great threat to human health and the environment. Given that different OPs present toxicology toxicities, identifying individual pesticide residues becomes important for assessing food safety environmental implications. In this work, kinetics difference-driven analyte hydrolysis strategy is proposed first time validated identify p-nitrophenyl by developing an organophosphorus hydrolase-like nanozyme-coded sensor array. Ultrasmall bare CeO2 nanoparticles were synthesized employed as only sensing unit catalyze of multiple analytes. With catalytic preferences differences under identical reaction conditions, five common analogues (methyl-paraoxon, paraoxon, methyl-parathion, parathion, fenitrothion) offered discriminable colors. By coupling color fingerprints with pattern recognition, accurate identification their mixtures at variety concentrations ratios was verified laboratory practical scenarios. Attractively, apart from excellent performance convenient operation, hydrolytic presents strong resistance against redox substances often cause interference previous oxidoreductase-based arrays. Our study provides new paradigm discriminating specific precisely, showing promising applications multitarget analysis complex matrices.

Language: Английский

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Ultrasmall Ce-based metal–organic frameworks nanozyme with hydrolytic activity for boosting antibiofilm therapy

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 480, P. 148246 - 148246

Published: Dec. 22, 2023

Language: Английский

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Single-atom nanozymes with peroxidase-like activity: A review

Chemosphere, Journal Year: 2023, Volume and Issue: 346, P. 140557 - 140557

Published: Oct. 28, 2023

Language: Английский

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Optical and Catalytic Properties of Nanozymes for Colorimetric Biosensors: Advantages, Limitations, and Perspectives

Advanced Optical Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Abstract Detection of colorimetric signals is commonly used in various analytical methods and for testing non‐laboratory resource‐limited settings. The performance assays largely based on nanoparticles their unique optical properties. Multifunctional combining enzyme‐like catalytic properties—known as nanozymes—hold great promise applications signal‐generating labels. However, the extensive focus properties leaves overlooked. In this article, use nanozymes reviewed relying inherent nanozymes, detection a catalytically‐formed product, changes caused by product. impact extinction coefficient reaction products, well kinetic parameters sensitivity limit assays, are quantitatively evaluated. Finally, existing limitations prospects biosensors summarized.

Language: Английский

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Computer-aided nanodrug discovery: recent progress and future prospects

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(18), P. 9059 - 9132

Published: Jan. 1, 2024

Nanodrugs, which utilise nanomaterials in disease prevention and therapy, have attracted considerable interest since their initial conceptualisation the 1990s. Substantial efforts been made to develop nanodrugs for overcoming limitations of conventional drugs, such as low targeting efficacy, high dosage toxicity, potential drug resistance. Despite significant progress that has nanodrug discovery, precise design or screening with desired biomedical functions prior experimentation remains a challenge. This is particularly case regard personalised precision nanodrugs, require simultaneous optimisation structures, compositions, surface functionalities nanodrugs. The development powerful computer clusters algorithms it possible overcome this challenge through

Language: Английский

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Multifamily Nanozymes for Sustainable and Eco-Friendly Marine Antifouling

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Marine pollution poses a serious threat to the ecological environment, destroys habitats, reduces biodiversity, and negatively impacts fisheries human health. Therefore, development of efficient marine antifouling strategies is great significance. This study introduces manganese selenide nanoflowers (MnSe NFs) as multifamily nanozymes, exhibiting phosphoesterase-, oxidase-, peroxidase-like activities. The catalytic mechanism this nanozyme was elucidated using density functional theory calculations spectroscopic analyses. Laboratory tests demonstrated that MnSe NFs exhibit strong efficacy against biofilms formed by Staphylococcus aureus Pseudomonas aeruginosa, achieving an antibacterial rate exceeding 99.999%. In scenarios, ship hulls coated with NF-based paints significantly inhibited biofilm formation for over 90 days, offering advantages in environmental friendliness, sustainability, cost-effectiveness. provides novel approach controlling highlights potential nanozymes sustainable eco-friendly solution technologies.

Language: Английский

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Tuning the Electronic Configuration of Oxygen Atom in Engineering Non‐Self‐Limited Nanozyme for Portable Immunosensor

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(10)

Published: Nov. 22, 2023

Abstract Nanozymes are conceived to replace natural enzymes avoid expensive processing techniques and fragile reservation conditions. Unfortunately, the self‐limited reaction has run counter desire for sustainable catalysis as enzymes. Herein, universal mechanism overcome self‐limitation of nanozymes caused by absorption ions on active sites is explored. Therefore, dual noble metals (Au/Pt) introduced center synthesize two kinds with different types central metal elements (Cu Ce) also compounds (metal‐organic framework metallic oxide), showing a similar bond between oxygen atom. The morphology characterization density functional theory calculations demonstrate that improvement in electronic environment neighboring atoms inducing charge redistribution via crucial enhancing catalytic activity avoiding adsorption nontarget substrate. This work not only experimentally proves adjustment adjacent feasible approach reaction, but achieves application self‐powered portable immunosensor broaden sensitive detection illegal addition food.

Language: Английский

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A Nucleophilicity‐Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract During fractionated radiotherapy, DNA damage repair intensifies in tumor cells, culminating cancer radioresistance and subsequent radiotherapy failure. Despite the recent development of nanoradiosensitizers targeting specific pathways, persistence mechanisms involving multiple pathways remains inevitable. To address this challenge, a nucleophilicity‐engineered ligation blockade nanoradiosensitizer (DLBN) comprising Au/CeO 2 heteronanostructure modified with trans‐acting activator transcription peptides is reported, which targets inhibits inside cell nuclei via heterointerface‐mediated dephosphorylation DNA, crucial step overcoming radioresistance. First, Schottky‐type nucleus‐targeting DLBN effectively radiation‐induced catalase‐mimetic activity radiation‐triggered catalytic reactions. Notably, by leveraging heterointerface, spontaneously dissociates H O to hydroxide, nucleophile higher nucleophilicity, thereby exhibiting remarkable capability at nicks through facilitated nucleophilic attack. This enables ligation, pivotal all interrupting process. Consequently, resensitizes radioresistant cells therapy‐induced radioresistance, leading substantial accumulation unrepaired damage. These findings offer insight into within nuclei, underscore potential heteronanostructure‐based block against

Language: Английский

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A novel fluorescent sensor for highly sensitive detection of ascorbic acid in food based on inhibiting phosphatase-like activity of Zr-based MOF

Food Chemistry, Journal Year: 2025, Volume and Issue: 471, P. 142837 - 142837

Published: Jan. 9, 2025

Language: Английский

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