Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(25), P. 9623 - 9629
Published: June 12, 2023
The
high
isomeric
complexity
of
glycans
makes
them
particularly
difficult
to
analyze.
While
ultra-high-resolution
ion
mobility
spectrometry
(IMS)
can
offer
rapid
baseline
separation
many
glycan
isomers,
their
unambiguous
identification
remains
a
challenging
task.
One
approach
solving
this
problem
is
identify
mobility-separated
isomers
by
measuring
highly
resolved
cryogenic
vibrational
spectra.
To
be
able
apply
complex
mixtures
at
throughput,
we
have
recently
developed
Hadamard
transform
multiplexed
spectroscopic
technique
that
allows
spectra
all
species
separated
in
both
IMS
and
mass
dimensions
single
laser
scan.
In
the
current
work,
further
develop
multiplexing
using
traps
incorporated
directly
into
device
based
on
structures
for
lossless
manipulations
(SLIM).
We
also
show
spectroscopy
perfect
sequence
matrices
outperform
standard
Simplex
matrices.
Lastly,
increase
measurement
speed
throughput
running
multiple
schemes
several
SLIM
combination
with
simultaneous
measurements
segmented
trap.
Microbiology and Molecular Biology Reviews,
Journal Year:
2024,
Volume and Issue:
88(1)
Published: Jan. 11, 2024
SUMMARY
Human
milk
oligosaccharides
(HMOs)
are
complex,
multi-functional
glycans
present
in
human
breast
milk.
They
represent
an
intricate
mix
of
heterogeneous
structures
which
reach
the
infant
intestine
intact
form
as
they
resist
gastrointestinal
digestion.
Therefore,
confer
a
multitude
benefits,
directly
and/or
indirectly,
to
developing
neonate.
Certain
bifidobacterial
species,
being
among
earliest
gut
colonizers
breast-fed
infants,
have
adapted
functional
capacity
metabolize
various
HMO
structures.
This
ability
is
typically
observed
infant-associated
bifidobacteria,
opposed
bifidobacteria
associated
with
mature
microbiota.
In
recent
years,
information
has
been
gleaned
regarding
how
these
well
certain
other
taxa
able
assimilate
HMOs,
including
mechanistic
strategies
enabling
their
acquisition
and
consumption.
Additionally,
complex
metabolic
interactions
occur
between
microbes
facilitated
by
utilization
breakdown
products
released
from
degradation.
Interest
HMO-mediated
changes
microbial
composition
function
focal
point
numerous
studies,
times
fueled
availability
individual
biosynthetic
some
now
commonly
included
formula.
this
review,
we
outline
main
assimilatory
catabolic
employed
discuss
that
exhibit
glycan
degradation
capacity,
cover
HMO-supported
cross-feeding
related
metabolites
described
thus
far.
Nutrients,
Journal Year:
2023,
Volume and Issue:
15(7), P. 1701 - 1701
Published: March 30, 2023
Prebiotics
are
substrates
that
selectively
utilized
by
host
microorganisms,
thus
conferring
a
health
benefit.
There
is
growing
awareness
interpersonal
and
age-dependent
differences
in
gut
microbiota
composition
impact
prebiotic
effects.
Due
to
the
interest
using
human
milk
oligosaccharides
(HMOs)
beyond
infancy,
this
study
evaluated
how
HMOs
[2'Fucosyllactose
(2'FL),
Lacto-N-neotetraose
(LNnT),
3'Sialyllactose
(3'SL),
6'Sialyllactose
(6'SL)]
blends
thereof
affect
of
6-year-old
children
(
FEMS Microbiology Reviews,
Journal Year:
2023,
Volume and Issue:
47(6)
Published: Oct. 4, 2023
Abstract
A
number
of
bacterial
species
are
found
in
high
abundance
the
faeces
healthy
breast-fed
infants,
an
occurrence
that
is
understood
to
be,
at
least
part,
due
ability
these
bacteria
metabolize
human
milk
oligosaccharides
(HMOs).
HMOs
third
most
abundant
component
after
lactose
and
lipids,
represent
complex
sugars
which
possess
unique
structural
diversity
resistant
infant
gastrointestinal
digestion.
Thus,
reach
distal
intestine
intact,
thereby
serving
as
a
fermentable
substrate
for
specific
intestinal
microbes,
including
Firmicutes,
Proteobacteria,
especially
infant-associated
Bifidobacterium
spp.
help
shape
gut
microbiome.
Bacteria
utilising
equipped
with
genes
associated
their
degradation
carbohydrate-active
enzymes
known
glycoside
hydrolase
have
been
identified
gut,
supports
this
hypothesis.
The
resulting
degraded
can
also
be
used
growth
substrates
other
present
microbe-microbe
interaction
‘cross-feeding’.
This
review
describes
current
knowledge
on
HMO
metabolism
by
particular
gut-associated
bacteria,
many
currently
commercial
probiotics,
distinct
strategies
employed
individual
utilisation.
Synthetic and Systems Biotechnology,
Journal Year:
2023,
Volume and Issue:
8(3), P. 509 - 519
Published: July 7, 2023
-Acetylneuraminic
acid
(Neu5Ac),
the
most
common
type
of
Sia,
generally
acts
as
terminal
sugar
in
cell
surface
glycans,
glycoconjugates,
oligosaccharides,
lipo-oligosaccharides,
and
polysaccharides,
thus
exerting
numerous
physiological
functions.
The
extensive
applications
Neu5Ac
food,
cosmetic,
pharmaceutical
industries
make
large-scale
production
this
chemical
desirable.
Biosynthesis
which
is
associated
with
important
application
potential
environmental
friendliness
has
become
an
indispensable
approach
for
synthesis
Neu5Ac.
In
review,
roles
was
first
summarized
detail.
Second,
safety
evaluation,
regulatory
status,
were
discussed.
Third,
enzyme-catalyzed
preparation,
whole-cell
biocatalysis,
microbial
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 11, 2025
Enterocolitis
is
a
common
and
potentially
deadly
manifestation
of
Hirschsprung
disease
(HSCR)
but
mechanisms
remain
poorly
defined.
Unexpectedly,
we
discovered
that
diet
can
dramatically
affect
the
lifespan
HSCR
mouse
model
(
Piebald
lethal
,
sl/sl
)
where
affected
animals
die
from
HAEC
complications.
In
model,
alters
gut
microbes
metabolites,
leading
to
changes
in
colon
epithelial
gene
expression
oxygen
levels
known
influence
colitis
severity.
Our
findings
demonstrate
unrecognized
similarity
between
other
types
suggest
dietary
manipulation
could
be
valuable
therapeutic
strategy
for
people
with
HSCR.
birth
defect
enteric
nervous
system
(ENS)
absent
distal
bowel.
Bowel
lacking
ENS
fails
relax,
causing
partial
obstruction.
Affected
children
often
have
"Hirschsprung
associated
enterocolitis"
(HAEC),
which
predisposes
sepsis.
We
survival
mice,
well-established
HAEC,
markedly
altered
by
two
distinct
standard
chow
diets.
A
"Protective"
increased
fecal
butyrate/isobutyrate
enhanced
production
antimicrobial
peptides
proximal
colon.
contrast,
"Detrimental"
diet-fed
had
abnormal
appearing
epithelium
mitochondria,
reduced
mRNA
involved
oxidative
phosphorylation,
elevated
fostered
growth
inflammation-associated
Enterobacteriaceae
.
Accordingly,
selective
depletion
sodium
tungstate
prolonged
survival.
results
provide
first
strong
evidence
modifies
without
altering
length
ENS.
Two
different
diets
alter
disease,
impacting
extent
aganglionosis
(the
region
ENS).
mice
fed
many
transcriptome
including
decreased
genes
phosphorylation.
Detrimental
also
aberrant-appearing
mitochondria
epithelium,
drives
overgrowth
via
aerobic
respiration.
Elimination
antibiotics
or
improves
"Detrimental
diet".
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
73(9), P. 5376 - 5384
Published: Feb. 18, 2025
Biological
production
of
human
milk
oligosaccharides
(HMOs)
using
metabolically
engineered
strains
is
a
research
hotspot
in
food
biotechnology,
but
less
effort
has
been
made
on
the
biological
sialylated
complex
HMOs.
Sialyllacto-N-tetraose
c
only
monosialylated
HMO
top
15
In
this
study,
metabolic
pathway
LST
was
constructed
Escherichia
coli
BL21(DE3)
by
introducing
three
sequential
glycosyltransferases:
β1,3-N-acetylglucosaminyltransferase,
β1,4-galactosyltransferase,
and
α2,6-sialyltransferase.
The
cytidine
5′-monophospho
(CMP)-N-acetylneuraminic
acid
(Neu5Ac)
enhanced
to
improve
production.
β1,4-galactosyltransferase
from
Helicobacter
pylori
J99
(HpGalT)
α2,6-sialyltransferase
Vespertiliibacter
pulmonis
(ED6ST)
were
screened
as
pair
key
glycosyltransferases
for
enhancing
final
strain
could
produce
1.718
9.745
g/L
shake-flask
fed-batch
cultivation,
respectively,
indicating
feasibility
efficient
biosynthesis
