Recent advances in biosynthesis mechanisms and yield enhancement strategies of erythritol DOI
Wen‐Li Yang,

Xiangying Zhao,

Mo Han

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 21

Published: Oct. 4, 2023

AbstractErythritol is a four-carbon sugar alcohol naturally produced by microorganisms as an osmoprotectant. As new substitute, erythritol has recently been popular on the ingredient market because of its unique nutritional characteristics. Even though history biosynthesis dates from turn twentieth century, scientific advancement lagged behind other polyols due to relative complexity making it. In recent years, biosynthetic methods for have rapidly developed increase in demand, better understanding metabolic pathways, and rapid development genetic engineering tools. This paper reviews industrial strain focuses underlying mechanism high production strains gained through screening or mutagenesis. Meanwhile, we highlight pathway knowledge summarize research progress critical genes involved different stages synthetic pathway. Lastly, talk about still-contentious issues promising future directions that will help break bottleneck make greener more sustainable.Keywords: Erythritolindustrial biotechnologymetabolic pathwayserythrose reductasetransketolaseYarrowia lipolytica AcknowledgmentsWe also thank PhD student Mingjing Yao her selfless submitting manuscript proofreading formatting.Disclosure statementNo potential conflict interest was reported author(s).Additional informationFundingThe author(s) appreciate financial support Natural Science Foundation Shandong Province (C010303) Major Innovation Pilot Project Integration Science, Education, Industry Qilu University Technology (Shandong Academy Science) (2022JBZ01-08).

Language: Английский

Enhancement of the d-Allulose 3-Epimerase Expression in Bacillus subtilis through Both Transcriptional and Translational Regulations DOI
Wenli Zhang, Hu Ren, Jiajun Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(14), P. 8052 - 8059

Published: April 2, 2024

d-Allulose, a functional bulk sweetener, has recently attracted increasing attention because of its low-caloric-ness properties and diverse health effects. d-Allulose is industrially produced by the enzymatic epimerization d-fructose, which catalyzed ketose 3-epimerase (KEase). In this study, food-grade expression KEase was studied using Bacillus subtills as host. Clostridium sp. d-allulose (Clsp-DAEase) screened from nine d-allulose-producing KEases, showing better potential for in B. WB600. Promoter-based transcriptional regulation N-terminal coding sequence (NCS)-based translational were to enhance DAEase level. addition, synergistic effect promoter NCS on Clsp-DAEase studied. Finally, strain with combination PHapII gln A-Up selected best Clsp-DAEase-producing strain. It efficiently total activity 333.2 1860.6 U/mL shake-flask fed-batch cultivations, respectively.

Language: Английский

Citations

9

Symbiotic bacteria and fungi proliferate in diapause and may enhance overwintering survival in a solitary bee DOI Creative Commons
Shawn M. Christensen,

Sriram N Srinivas,

Quinn S. McFrederick

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Host-microbe interactions underlie the development and fitness of many macroorganisms, including bees. Whereas social bees benefit from vertically transmitted gut bacteria, current data suggests that solitary bees, which comprise vast majority species diversity within lack a highly specialized microbiome. Here, we examine composition abundance bacteria fungi throughout complete life cycle ground-nesting bee Anthophora bomboides standfordiana. In contrast to expectations, immature stages maintain distinct core microbiome consisting Actinobacterial genera (Streptomyces, Nocardiodes) fungus Moniliella spathulata. Dormant (diapausing) larval hosted most abundant distinctive fungi, attaining 33 52 times their initial copy number, respectively. We tested two adaptive hypotheses regarding microbial functions for diapausing First, using isolated found Streptomyces brood cells inhibited growth multiple pathogenic filamentous suggesting role in pathogen protection during overwintering, when face high pressure. Second, sugar alcohol changed tandem with major changes fungal abundance, links cold tolerance or overwintering biology. find A. hosts conserved may provide key advantages through diapause, raises question how this is maintained faithfully between generations. Our results suggest focus on microbiomes mature active insect developmental overlook stage-specific symbionts contributions host dormancy.

Language: Английский

Citations

7

Bifunctional organocatalyst-catalyzed dynamic kinetic resolution of hemiketals for synthesis of chiral ketals via hydrogen bonding control DOI

Meng Shan,

Yongmei Yu,

Mengli Sun

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: 36(1), P. 109781 - 109781

Published: March 15, 2024

Language: Английский

Citations

6

Surfactants produced from carbohydrate derivatives: Part 2. A review on the value chain, synthesis, and the potential role of artificial intelligence within the biorefinery concept DOI Creative Commons
Ronald Márquez, Maria Soledad Ortiz, Nelson Barrios

et al.

Journal of Surfactants and Detergents, Journal Year: 2024, Volume and Issue: unknown

Published: May 21, 2024

Abstract This comprehensive and critical review explores the synthesis applications of carbohydrate‐based surfactants within biorefinery concept, focusing on biobased sugar‐head molecules suitable for use across several manufacturing sectors, including cosmetics, pharmaceuticals, household products, detergents, foods. The main focus relies sustainable alternatives to conventional surfactants, which could reduce final carbon footprint industrial feedstocks products. A thorough analysis raw materials, highlighting significance feedstock sources, current rhamnolipid biosurfactants production trends, is presented. Key organic reactions sorbitan esters, sucrose alkyl polyglycosides, fatty acid glucamines, such as glycosidation, acylation, etherification, well rhamnolipids through fermentation are described. Given scarce literature characterization these surfactant types hydrophilic–lipophilic deviation (HLD) framework, contribution parameter (SCP) in HLD equation critically assessed. economic landscape also discussed, noting significant growth biosurfactant market, driven by environmental awareness regulatory changes, with projections indicating a substantial market increase forthcoming years. Finally, promising potential generative artificial intelligence (AI) developing customized molecules, optimized properties targeted applications, emphasized avenue future research.

Language: Английский

Citations

5

Recent progress of low-temperature plasma technology in biorefining process DOI Creative Commons

Lusha Qin,

Oi Lun Li

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Aug. 24, 2023

Abstract In recent years, low-temperature plasma-assisted processes, featuring high reaction efficiency and wide application scope, have emerged as a promising alternative to conventional methods for biomass valorization. It is well established that charged species, chemically energetic molecules radicals, highly active photons playing key roles during processing. This review presents the major applications of plasma conversion in terms (i) pretreatment biomass, (ii) chemo fractionation into value-added chemicals, (iii) synthesis heterogeneous catalyst further chemo-catalytic conversion. The first foremost step upgrading facilitate raw transformation, which reduces crystallinity, purification, delignification. involves primary reactions various kinds target products, such hydrolysis, hydrogenation, retro-aldol condensation so on. Finally, researches on chemo-catalysis catalysts fabricated via at relatively mild condition were introduced. These reported with comparable performance other state-of-the-art prepared using methods. Graphical

Language: Английский

Citations

12

Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica DOI
Fangmei Liu, Kai Xia, Yuqing Chen

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: 121(6), P. 1937 - 1949

Published: March 28, 2024

Abstract This study aimed to investigate the effect of hyphal formation in Yarrowia lipolytica and biochar addition on erythritol production by submerged fermentation. Hyphal significantly inhibited Y. . Transcriptome analysis suggested that impaired synthesis cells was associated with differential expression genes involved amino acid metabolism, lipid cell wall stability. Deletion RAS2 responsible for yeast‐to‐hypha transition EYD1 included degradation blocked improved production. Biochar prepared from corncob, sugarcane bagasse (SB), corn straw, peanut shell, coconut walnut shell (WS) had a positive production, which WS pyrolyzed at 500°C (WSc) performed best flask In 3.7 L bioreactor, 220.20 ± 10 g/L productivity 2.30 0.10 g/L/h obtained presence 1.4% (w/v) WSc 0.7% SBc (SB 500°C) within 96 h. These results suggest inhibition together is an efficient way promote

Language: Английский

Citations

4

Production of Sorbitol via Hydrogenation of Glucose over Ruthenium Coordinated with Amino Styrene-co-maleic Anhydride Polymer Encapsulated on Activated Carbon (Ru/ASMA@AC) Catalyst DOI Creative Commons

Xiaorui Yang,

Xiaotong Li,

Jing Zhao

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(12), P. 4830 - 4830

Published: June 17, 2023

Sorbitol, a product primarily derived from glucose hydrogenation, has extensive applications in the pharmaceutical, chemical and other industries. Amino styrene-co-maleic anhydride polymer encapsulated on activated carbon (Ru/ASMA@AC) catalysts were developed for efficient hydrogenation prepared confined Ru by coordination with (ASMA). Through single-factor experiments, optimal conditions determined to be 2.5 wt.% ruthenium loading catalyst usage of 1.5 g, 20% solution at 130 °C, reaction pressure 4.0 MPa, stirring speed 600 rpm 3 h. These achieved high conversion rate 99.68% sorbitol selectivity 93.04%. Reaction kinetics testing proved that catalyzed Ru/ASMA@AC was first-order reaction, activation energy 73.04 kJ/mol. Furthermore, catalytic performance Ru/AC compared characterized various detection methods. The exhibited excellent stability after five cycles, whereas traditional suffered 10% decrease yield three cycles. results suggest is more promising candidate high-concentration due its superior stability.

Language: Английский

Citations

10

Enhanced comprehensive thermal performance of ternary composite phase change materials with graphite powder hybridizing expanded graphite/erythritol DOI

Kuerbanjiang Wusiman,

Xiaoye Dai,

Lin Shi

et al.

International Journal of Green Energy, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: Feb. 4, 2025

Language: Английский

Citations

0

The effects of lecithin on the complexation between pea starch and aliphatic alcohols in aqueous medium DOI
Xiaoyang He, Yifan Wang,

Liyang Zhou

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 475, P. 143364 - 143364

Published: Feb. 12, 2025

Language: Английский

Citations

0

Synergistic effect of transporter and pathway engineering on the key performance indicators of erythritol synthesis by the yeast Yarrowia lipolytica DOI Creative Commons
Shuo Xu,

Qian Li,

Ye Li

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

ABSTRACT Erythritol, a food additive, is produced on an industrial scale using the yeast Yarrowia lipolytica . Nevertheless, key performance indicators (KPIs) have been found to be unsatisfactory, resulting in elevated erythritol production cost. This study demonstrated that KPIs (titer, productivity, and yield) of can improved by synergistic application transporter pathway engineering strategies producing strain. The engineered Y. strain Ylxs48 exhibits glucose consumption rate 310 g/L within 46 h during batch culture 3, 100, 200 L bioreactors as compared above 72 for parental Ylxs01. yield achieved ranges from 0.69 0.74 g/g depending conditions 0.55–0.57 productivity surpasses 4.60 g/(L·h), representing 1.91-fold improvement over Ylxs01 or bioreactors. Under fed-batch bioreactor, titer 355.81 was achieved, marking highest ever reported. increased enabled crystallization at 4°C directly clear supernatant, eliminating requirement evaporation concentration steps. A comprehensive techno-economic analysis entire process conclusively implementing based led significant 23% reduction approach holds potential substantially reduce costs provides novel insights other strains. IMPORTANCE expansion market attracted excessive capital injection, overcapacity, operational difficulties, even bankruptcy manufacturers. Technology upgrades industry are imminent. However, technology existing enterprises seriously homogenized, there lack competitive core-producing In this study, erythritol-producing CGMCC7326 genetically modified integrating substrate transport modification, which conversion significantly KPIs, thereby reducing

Language: Английский

Citations

0