Critical Reviews in Food Science and Nutrition,
Journal Year:
2023,
Volume and Issue:
unknown, P. 1 - 21
Published: Oct. 4, 2023
AbstractErythritol
is
a
four-carbon
sugar
alcohol
naturally
produced
by
microorganisms
as
an
osmoprotectant.
As
new
substitute,
erythritol
has
recently
been
popular
on
the
ingredient
market
because
of
its
unique
nutritional
characteristics.
Even
though
history
biosynthesis
dates
from
turn
twentieth
century,
scientific
advancement
lagged
behind
other
polyols
due
to
relative
complexity
making
it.
In
recent
years,
biosynthetic
methods
for
have
rapidly
developed
increase
in
demand,
better
understanding
metabolic
pathways,
and
rapid
development
genetic
engineering
tools.
This
paper
reviews
industrial
strain
focuses
underlying
mechanism
high
production
strains
gained
through
screening
or
mutagenesis.
Meanwhile,
we
highlight
pathway
knowledge
summarize
research
progress
critical
genes
involved
different
stages
synthetic
pathway.
Lastly,
talk
about
still-contentious
issues
promising
future
directions
that
will
help
break
bottleneck
make
greener
more
sustainable.Keywords:
Erythritolindustrial
biotechnologymetabolic
pathwayserythrose
reductasetransketolaseYarrowia
lipolytica
AcknowledgmentsWe
also
thank
PhD
student
Mingjing
Yao
her
selfless
submitting
manuscript
proofreading
formatting.Disclosure
statementNo
potential
conflict
interest
was
reported
author(s).Additional
informationFundingThe
author(s)
appreciate
financial
support
Natural
Science
Foundation
Shandong
Province
(C010303)
Major
Innovation
Pilot
Project
Integration
Science,
Education,
Industry
Qilu
University
Technology
(Shandong
Academy
Science)
(2022JBZ01-08).
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(14), P. 8052 - 8059
Published: April 2, 2024
d-Allulose,
a
functional
bulk
sweetener,
has
recently
attracted
increasing
attention
because
of
its
low-caloric-ness
properties
and
diverse
health
effects.
d-Allulose
is
industrially
produced
by
the
enzymatic
epimerization
d-fructose,
which
catalyzed
ketose
3-epimerase
(KEase).
In
this
study,
food-grade
expression
KEase
was
studied
using
Bacillus
subtills
as
host.
Clostridium
sp.
d-allulose
(Clsp-DAEase)
screened
from
nine
d-allulose-producing
KEases,
showing
better
potential
for
in
B.
WB600.
Promoter-based
transcriptional
regulation
N-terminal
coding
sequence
(NCS)-based
translational
were
to
enhance
DAEase
level.
addition,
synergistic
effect
promoter
NCS
on
Clsp-DAEase
studied.
Finally,
strain
with
combination
PHapII
gln
A-Up
selected
best
Clsp-DAEase-producing
strain.
It
efficiently
total
activity
333.2
1860.6
U/mL
shake-flask
fed-batch
cultivations,
respectively.
The ISME Journal,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Jan. 1, 2024
Host-microbe
interactions
underlie
the
development
and
fitness
of
many
macroorganisms,
including
bees.
Whereas
social
bees
benefit
from
vertically
transmitted
gut
bacteria,
current
data
suggests
that
solitary
bees,
which
comprise
vast
majority
species
diversity
within
lack
a
highly
specialized
microbiome.
Here,
we
examine
composition
abundance
bacteria
fungi
throughout
complete
life
cycle
ground-nesting
bee
Anthophora
bomboides
standfordiana.
In
contrast
to
expectations,
immature
stages
maintain
distinct
core
microbiome
consisting
Actinobacterial
genera
(Streptomyces,
Nocardiodes)
fungus
Moniliella
spathulata.
Dormant
(diapausing)
larval
hosted
most
abundant
distinctive
fungi,
attaining
33
52
times
their
initial
copy
number,
respectively.
We
tested
two
adaptive
hypotheses
regarding
microbial
functions
for
diapausing
First,
using
isolated
found
Streptomyces
brood
cells
inhibited
growth
multiple
pathogenic
filamentous
suggesting
role
in
pathogen
protection
during
overwintering,
when
face
high
pressure.
Second,
sugar
alcohol
changed
tandem
with
major
changes
fungal
abundance,
links
cold
tolerance
or
overwintering
biology.
find
A.
hosts
conserved
may
provide
key
advantages
through
diapause,
raises
question
how
this
is
maintained
faithfully
between
generations.
Our
results
suggest
focus
on
microbiomes
mature
active
insect
developmental
overlook
stage-specific
symbionts
contributions
host
dormancy.
Journal of Surfactants and Detergents,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 21, 2024
Abstract
This
comprehensive
and
critical
review
explores
the
synthesis
applications
of
carbohydrate‐based
surfactants
within
biorefinery
concept,
focusing
on
biobased
sugar‐head
molecules
suitable
for
use
across
several
manufacturing
sectors,
including
cosmetics,
pharmaceuticals,
household
products,
detergents,
foods.
The
main
focus
relies
sustainable
alternatives
to
conventional
surfactants,
which
could
reduce
final
carbon
footprint
industrial
feedstocks
products.
A
thorough
analysis
raw
materials,
highlighting
significance
feedstock
sources,
current
rhamnolipid
biosurfactants
production
trends,
is
presented.
Key
organic
reactions
sorbitan
esters,
sucrose
alkyl
polyglycosides,
fatty
acid
glucamines,
such
as
glycosidation,
acylation,
etherification,
well
rhamnolipids
through
fermentation
are
described.
Given
scarce
literature
characterization
these
surfactant
types
hydrophilic–lipophilic
deviation
(HLD)
framework,
contribution
parameter
(SCP)
in
HLD
equation
critically
assessed.
economic
landscape
also
discussed,
noting
significant
growth
biosurfactant
market,
driven
by
environmental
awareness
regulatory
changes,
with
projections
indicating
a
substantial
market
increase
forthcoming
years.
Finally,
promising
potential
generative
artificial
intelligence
(AI)
developing
customized
molecules,
optimized
properties
targeted
applications,
emphasized
avenue
future
research.
Nano Convergence,
Journal Year:
2023,
Volume and Issue:
10(1)
Published: Aug. 24, 2023
Abstract
In
recent
years,
low-temperature
plasma-assisted
processes,
featuring
high
reaction
efficiency
and
wide
application
scope,
have
emerged
as
a
promising
alternative
to
conventional
methods
for
biomass
valorization.
It
is
well
established
that
charged
species,
chemically
energetic
molecules
radicals,
highly
active
photons
playing
key
roles
during
processing.
This
review
presents
the
major
applications
of
plasma
conversion
in
terms
(i)
pretreatment
biomass,
(ii)
chemo
fractionation
into
value-added
chemicals,
(iii)
synthesis
heterogeneous
catalyst
further
chemo-catalytic
conversion.
The
first
foremost
step
upgrading
facilitate
raw
transformation,
which
reduces
crystallinity,
purification,
delignification.
involves
primary
reactions
various
kinds
target
products,
such
hydrolysis,
hydrogenation,
retro-aldol
condensation
so
on.
Finally,
researches
on
chemo-catalysis
catalysts
fabricated
via
at
relatively
mild
condition
were
introduced.
These
reported
with
comparable
performance
other
state-of-the-art
prepared
using
methods.
Graphical
Biotechnology and Bioengineering,
Journal Year:
2024,
Volume and Issue:
121(6), P. 1937 - 1949
Published: March 28, 2024
Abstract
This
study
aimed
to
investigate
the
effect
of
hyphal
formation
in
Yarrowia
lipolytica
and
biochar
addition
on
erythritol
production
by
submerged
fermentation.
Hyphal
significantly
inhibited
Y.
.
Transcriptome
analysis
suggested
that
impaired
synthesis
cells
was
associated
with
differential
expression
genes
involved
amino
acid
metabolism,
lipid
cell
wall
stability.
Deletion
RAS2
responsible
for
yeast‐to‐hypha
transition
EYD1
included
degradation
blocked
improved
production.
Biochar
prepared
from
corncob,
sugarcane
bagasse
(SB),
corn
straw,
peanut
shell,
coconut
walnut
shell
(WS)
had
a
positive
production,
which
WS
pyrolyzed
at
500°C
(WSc)
performed
best
flask
In
3.7
L
bioreactor,
220.20
±
10
g/L
productivity
2.30
0.10
g/L/h
obtained
presence
1.4%
(w/v)
WSc
0.7%
SBc
(SB
500°C)
within
96
h.
These
results
suggest
inhibition
together
is
an
efficient
way
promote
Molecules,
Journal Year:
2023,
Volume and Issue:
28(12), P. 4830 - 4830
Published: June 17, 2023
Sorbitol,
a
product
primarily
derived
from
glucose
hydrogenation,
has
extensive
applications
in
the
pharmaceutical,
chemical
and
other
industries.
Amino
styrene-co-maleic
anhydride
polymer
encapsulated
on
activated
carbon
(Ru/ASMA@AC)
catalysts
were
developed
for
efficient
hydrogenation
prepared
confined
Ru
by
coordination
with
(ASMA).
Through
single-factor
experiments,
optimal
conditions
determined
to
be
2.5
wt.%
ruthenium
loading
catalyst
usage
of
1.5
g,
20%
solution
at
130
°C,
reaction
pressure
4.0
MPa,
stirring
speed
600
rpm
3
h.
These
achieved
high
conversion
rate
99.68%
sorbitol
selectivity
93.04%.
Reaction
kinetics
testing
proved
that
catalyzed
Ru/ASMA@AC
was
first-order
reaction,
activation
energy
73.04
kJ/mol.
Furthermore,
catalytic
performance
Ru/AC
compared
characterized
various
detection
methods.
The
exhibited
excellent
stability
after
five
cycles,
whereas
traditional
suffered
10%
decrease
yield
three
cycles.
results
suggest
is
more
promising
candidate
high-concentration
due
its
superior
stability.
Applied and Environmental Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 26, 2025
ABSTRACT
Erythritol,
a
food
additive,
is
produced
on
an
industrial
scale
using
the
yeast
Yarrowia
lipolytica
.
Nevertheless,
key
performance
indicators
(KPIs)
have
been
found
to
be
unsatisfactory,
resulting
in
elevated
erythritol
production
cost.
This
study
demonstrated
that
KPIs
(titer,
productivity,
and
yield)
of
can
improved
by
synergistic
application
transporter
pathway
engineering
strategies
producing
strain.
The
engineered
Y.
strain
Ylxs48
exhibits
glucose
consumption
rate
310
g/L
within
46
h
during
batch
culture
3,
100,
200
L
bioreactors
as
compared
above
72
for
parental
Ylxs01.
yield
achieved
ranges
from
0.69
0.74
g/g
depending
conditions
0.55–0.57
productivity
surpasses
4.60
g/(L·h),
representing
1.91-fold
improvement
over
Ylxs01
or
bioreactors.
Under
fed-batch
bioreactor,
titer
355.81
was
achieved,
marking
highest
ever
reported.
increased
enabled
crystallization
at
4°C
directly
clear
supernatant,
eliminating
requirement
evaporation
concentration
steps.
A
comprehensive
techno-economic
analysis
entire
process
conclusively
implementing
based
led
significant
23%
reduction
approach
holds
potential
substantially
reduce
costs
provides
novel
insights
other
strains.
IMPORTANCE
expansion
market
attracted
excessive
capital
injection,
overcapacity,
operational
difficulties,
even
bankruptcy
manufacturers.
Technology
upgrades
industry
are
imminent.
However,
technology
existing
enterprises
seriously
homogenized,
there
lack
competitive
core-producing
In
this
study,
erythritol-producing
CGMCC7326
genetically
modified
integrating
substrate
transport
modification,
which
conversion
significantly
KPIs,
thereby
reducing