Advances in 3D bioprinting for regenerative medicine applications DOI Creative Commons

Konstantinos Loukelis,

Nikos Koutsomarkos,

Antonios G. Mikos

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

Abstract Biofabrication techniques allow for the construction of biocompatible and biofunctional structures composed from biomaterials, cells biomolecules. Bioprinting is an emerging 3D printing method which utilizes biomaterial-based mixtures with other biological constituents into printable suspensions known as bioinks. Coupled automated design protocols based on different modes droplet deposition, bioprinters are able to fabricate hydrogel-based objects specific architecture geometrical properties, providing necessary environment that promotes cell growth directs differentiation towards application-related lineages. For preparation such bioinks, various water-soluble biomaterials have been employed, including natural synthetic biopolymers, inorganic materials. Bioprinted constructs considered be one most promising avenues in regenerative medicine due their native organ biomimicry. a successful application, bioprinted should meet particular criteria optimal response, mechanical properties similar target tissue, high levels reproducibility fidelity, but also increased upscaling capability. In this review, we highlight recent advances bioprinting, focusing regeneration tissues bone, cartilage, cardiovascular, neural, skin organs liver, kidney, pancreas lungs. We discuss rapidly developing co-culture bioprinting systems used resemble complexity crosstalk between populations regeneration. Moreover, report basic physical principles governing ideal bioink biomaterials’ potential. examine critically present status regarding its applicability current limitations need overcome establish it at forefront artificial production transplantation.

Language: Английский

Polymeric biomaterials for 3D printing in medicine: An overview DOI Creative Commons
Raffaele Pugliese, Benedetta Beltrami, Stefano Regondi

et al.

Annals of 3D Printed Medicine, Journal Year: 2021, Volume and Issue: 2, P. 100011 - 100011

Published: April 9, 2021

Three-dimensional (3D) printing is becoming a booming technology to fabricate scaffolds, orthoses, and prosthetic devices for tissue engineering, regenerative medicine, rehabilitation patients with disabling neurological diseases (such as amyotrophic lateral sclerosis, traumatic brain injuries, spinal cord injuries). This due the potential of 3D provide patient-specific designs, high structural complexity, rapid on-demand fabrication at low-cost. However, one major bottlenecks that limits widespread acceptance biomedical manufacturing lack polymers, biomaterials, hydrogels, bioinks functional printing, biocompatible, more performing from biomechanical point view meet different needs. As matter fact field still struggling processing such materials into self-supporting tunable biomechanics, optimal structures, degradation, bioactivity. Here, will be highlighted all recent advances have been made in biomedicine, analyzing bioinks, according their printability, ease processability, cost, properties mechanics, biocompatibility, degradation rate. Finally, future considerations bio-fabrication discussed.

Language: Английский

Citations

141
On the progress of hydrogel-based 3D printing: Correlating rheological properties with printing behaviour DOI Creative Commons
Sara Bom, Ricardo Ribeiro, Helena Margarida Ribeiro

et al.

International Journal of Pharmaceutics, Journal Year: 2022, Volume and Issue: 615, P. 121506 - 121506

Published: Jan. 24, 2022

One of the exciting future directions in 3D printing field is development innovative personalized smart constructions for bio-applications, including drug delivery, namely high-throughput screening and customized topical/oral administration pharmaceuticals, as well tissue engineering. In this context, hydrogels have emerged a promising material that, when combined with extrusion printing, allow creation soft-material structures defined spatial locations, that can be printed at room temperature by tuning geometric design and/or formulation components. Thus, efficacy quality such vehicles dependent on formulation, design, process parameters. However, hydrogel inks are often designed characterized using different methods lack uniformity impairs. Characterization techniques usually arbitrary differ among research groups, challenging inference possible conclusions behaviour potential applications. Therefore, to properly analyse particular ink we review, first time, most frequently employed characterization procedures, from rheological approaches parameters settings, discuss their relevance, limitations drawbacks, highlight perspectives. Overall, accelerate high-quality constructs, comprehensive protocols both pre-printing assays should adopted. Furthermore, transversal adoption could serve boost terms requirements regulatory aspects.

Language: Английский

Citations

130
Hydrogels for Tissue Engineering: Addressing Key Design Needs Toward Clinical Translation DOI Creative Commons
Fei Xu, Chloe Dawson,

Makenzie Lamb

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: May 5, 2022

Graphical Abstract

Language: Английский

Citations

97
3D printing of bio-instructive materials: Toward directing the cell DOI Creative Commons
Piotr Stanisław Zieliński, Pavan Kumar Reddy Gudeti,

Timo Rikmanspoel

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 19, P. 292 - 327

Published: April 23, 2022

Fabrication of functional scaffolds for tissue engineering and regenerative medicine applications requires material systems with precise control over cellular performance. 3D printing is a powerful technique to create highly complex multicomponent structures well-defined architecture composition. In this review paper, we explore extrusion-based methods (EBP, i.e., Near Field Electrospinning (NFES), Melt Electrowriting (MEW), Fused Deposition Modeling (FDM), extrusion bioprinting) in terms their ability produce bio-instructive properties. These provide spatio-temporal guidance cells, allowing controlled regeneration maturation. Multiple physical biochemical cues introduced the EBP are evaluated direct cell alignment, proliferation, differentiation, specific ECM production, We indicate that have different impacts depending on system, type used, or coexistence multiple cues. Therefore, they must be carefully chosen based targeted application. propose future directions materials development, including such concepts as metamaterials, hybrid living materials, 4D printing. The gathers knowledge essential designing new response, fabrication advanced engineered tissue, developing better understanding biology, especially response biomaterial.

Language: Английский

Citations

81
Progress of Microfluidic Hydrogel‐Based Scaffolds and Organ‐on‐Chips for the Cartilage Tissue Engineering DOI
Hamidreza Tolabi, Niyousha Davari, Mehran Khajehmohammadi

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(26)

Published: Jan. 12, 2023

Abstract Cartilage degeneration is among the fundamental reasons behind disability and pain across globe. Numerous approaches have been employed to treat cartilage diseases. Nevertheless, none shown acceptable outcomes in long run. In this regard, convergence of tissue engineering microfabrication principles can allow developing more advanced microfluidic technologies, thus offering attractive alternatives current treatments traditional constructs used applications. Herein, developments involving hydrogel‐based scaffolds, promising structures for regeneration, ranging from hydrogels with channels prepared by devices, that enable therapeutic delivery cells, drugs, growth factors, as well cartilage‐related organ‐on‐chips are reviewed. Thereafter, anatomy types damages, present treatment options briefly overviewed. Various introduced, advantages scaffolds over thoroughly discussed. Furthermore, available technologies fabricating chips presented. The preclinical clinical applications regeneration development time further explained. developments, recent key challenges, prospects should be considered so develop systems repair highlighted.

Language: Английский

Citations

72
Natural and Synthetic Polymeric Biomaterials for Application in Wound Management DOI Creative Commons

Sabrina Prete,

Marco Dattilo, Francesco Patitucci

et al.

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(9), P. 455 - 455

Published: Sept. 3, 2023

Biomaterials are at the forefront of future, finding a variety applications in biomedical field, especially wound healing, thanks to their biocompatible and biodegradable properties. Wounds spontaneously try heal through series interconnected processes involving several initiators mediators such as cytokines, macrophages, fibroblasts. The combination biopolymers with healing properties may provide opportunities synthesize matrices that stimulate trigger target cell responses crucial process. This review outlines optimal management care required for treatment special focus on biopolymers, drug-delivery systems, nanotechnologies used enhanced applications. Researchers have utilized range techniques produce dressings, leading products different characteristics. Each method comes its unique strengths limitations, which important consider. future trajectory dressing advancement should prioritize economical eco-friendly methodologies, along improving efficacy constituent materials. aim this work is give researchers possibility evaluate proper materials preparation better understand synthesis conditions well most effective bioactive molecules load.

Language: Английский

Citations

65
Microbial cellulase production and its potential application for textile industries DOI Creative Commons
Gamachis Korsa, Rocktotpal Konwarh, Chandran Masi

et al.

Annals of Microbiology, Journal Year: 2023, Volume and Issue: 73(1)

Published: April 10, 2023

Abstract Purpose The textile industry’s previous chemical use resulted in thousands of practical particulate emissions, such as machine component damage and drainage system blockage, both which have implications. Enzyme-based processing is cost-effective, environmentally friendly, non-hazardous, water-saving. purpose this review to give evidence on the potential activity microbial cellulase industry, mostly confined realm research. Methods This was progressive by considering peer-reviewed papers linked production, its prospective application for industries appraised produced develop assessment. Articles were divided into two categories based results trustworthy educational journals: methods used produce diversity microorganisms through fermentation processes approaches microbes fermentation. Submerged (SMF) solid-state (SSF) techniques are currently being meet industrial demand production bio industry. Results Microbial vital increasing day due no side effect environment human health becoming increasingly important. In conventional processing, gray cloth subjected a series treatments that involved breaking dye molecule’s amino group with Cl − , started accelerated dye(-resistant) bond cracking. A enzyme primarily derived from variety species found various ecological settings biotextile/bio-based product technology future needs applications. Conclusion Cellulase has been advantages cellulose-based textiles, well quality enhancement fabric maintenance over traditional approaches. Cellulase’s role industry chosen an appropriate sound solution long healthy lifestyle.

Language: Английский

Citations

60
Advancing 3D bioprinting through machine learning and artificial intelligence DOI
Srikanthan Ramesh, Akash Deep, Ali Tamayol

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 38, P. e00331 - e00331

Published: Jan. 28, 2024

Language: Английский

Citations

35
Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications DOI
Sushila Maharjan,

Chenshuo Ma,

Bibhor Singh

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

Citations

30
Volumetric additive manufacturing: A new frontier in layer-less 3D printing DOI Creative Commons
Daniel J. Whyte, Egan H. Doeven, Alessandra Sutti

et al.

Additive manufacturing, Journal Year: 2024, Volume and Issue: 84, P. 104094 - 104094

Published: March 1, 2024

Volumetric Additive Manufacturing (VAM) is an emerging 3D printing technology that operates by fabricating objects from all points within a medium's volume. This technique capable of producing parts without supporting structures and overprinting around existing structures. Notably, the approach VAM utilises to print time efficient compared traditional additive manufacturing methods with times being measured in seconds minutes instead hours. As this there little comparison or synthesis reported so far literature, thus primary objective review address issue providing comprehensive analysis VAM, delving into its applications, challenges it faces, research advancements made area. also investigates how new are investigated on, literature. In order achieve goal structured literature was conducted thoroughly examine current state VAM. found 30 papers, which were used categorise different methods, explore potential various fields, formulate definition for differentiate other technologies. A key finding while offers rapid fabrication capabilities, currently faces several constraints. These include limited availability commercial printers, complex methodologies, restricted range compatible materials, need specialised equipment. Collectively, these factors could serve as barriers broader adoption technology. addition, lack homogeneity parameters investigate report makes difficult compare contrast works against published field progresses, addressing will be essential unlock applications increase one first explicitly focus on entirety offering valuable insights present directions future research.

Language: Английский

Citations

21