The Regulatory Role of MicroRNAs in Breast Cancer

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(19), P. 4940 - 4940

Published: Oct. 6, 2019

MicroRNAs (miRNAs) are small non-coding RNA molecules which function as critical post-transcriptional gene regulators of various biological functions. Generally, miRNAs negatively regulate expression by binding to their selective messenger RNAs (mRNAs), thereby leading either mRNA degradation or translational repression, depending on the degree complementarity with target sequences. Aberrant these has been linked etiologically human diseases including breast cancer. Different cellular pathways cancer development such cell proliferation, apoptotic response, metastasis, recurrence and chemoresistance regulated oncogenic miRNA (oncomiR) tumor suppressor (tsmiR). In this review, we highlight current state research into involved in cancer, particular attention articles published between years 2000 2019, using detailed searches databases PubMed, Google Scholar, Scopus. The regulatory roles dysregulated potential therapeutic targets also discussed.

Language: Английский

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Roles and mechanisms of exosomal non-coding RNAs in human health and diseases

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: Nov. 10, 2021

Exosomes play a role as mediators of cell-to-cell communication, thus exhibiting pleiotropic activities to homeostasis regulation. Exosomal non-coding RNAs (ncRNAs), mainly microRNAs (miRNAs), long (lncRNAs), and circular (circRNAs), are closely related variety biological functional aspects human health. When the exosomal ncRNAs undergo tissue-specific changes due diverse internal or external disorders, they can cause tissue dysfunction, aging, diseases. In this review, we comprehensively discuss underlying regulatory mechanisms exosomes in addition, explore current knowledge on roles miRNAs, lncRNAs, circRNAs health diseases, including cancers, metabolic neurodegenerative cardiovascular autoimmune infectious determine their potential implication biomarker identification therapeutic exploration.

Language: Английский

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Traditional herbal medicine: Therapeutic potential in rheumatoid arthritis

Journal of Ethnopharmacology, Journal Year: 2021, Volume and Issue: 279, P. 114368 - 114368

Published: June 29, 2021

Language: Английский

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Exosomes as Rheumatoid Arthritis Diagnostic Biomarkers and Therapeutic Agents

Vaccines, Journal Year: 2023, Volume and Issue: 11(3), P. 687 - 687

Published: March 17, 2023

Rheumatoid arthritis (RA) is a chronic inflammatory joint disorder that causes systemic inflammation, autoimmunity, and abnormalities result in permanent disability. Exosomes are nanosized extracellular particles found mammals (40–100 nm). They transporter of lipids, proteins, genetic material involved mammalian cell–cell signaling, biological processes, cell signaling. have been identified as playing role rheumatoid arthritis-related inflammation (RA). Uniquely functioning vesicles (EVs) responsible for the transport autoantigens mediators between distant cells. In addition, paracrine factors, such exosomes, modulate immunomodulatory function mesenchymal stem cells (MSCs). addition to transporting information, exosomes convey miRNAs studied drug delivery vehicles. animal models, it has observed MSCs secrete EVs with properties, promising results this area. By understanding diversity exosomal contents their corresponding targets, may be possible diagnose autoimmune diseases. can employed diagnostic biomarkers immunological disorders. We here discuss most recent findings regarding diagnostic, prognostic, therapeutic potential these nanoparticles provide an overview evidence pertaining biology RA.

Language: Английский

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MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression

Neural Regeneration Research, Journal Year: 2019, Volume and Issue: 15(4), P. 606 - 606

Published: Oct. 18, 2019

Multiple sclerosis is a chronic autoimmune disease of the central nervous system. It main cause non-traumatic neurological disability in young adults. mostly affects people aged 20-50 years; however, it can occur children and much older Factors identified distribution MS include age, gender, genetics, environment, ethnic background. usually associated with progressive degrees disability. The involves demyelination axons system causes brain spinal cord neuronal loss atrophy. Diagnosing multiple based on patient's medical history including symptoms, physical examination, various tests such as magnetic resonance imaging, cerebrospinal fluid blood tests, electrophysiology. course not well correlated biomarkers presently used clinical practice. Blood-derived that detect distinguish different phenotypes may be advantageous personalized treatment disease-modifying drugs to predict response treatment. studies reviewed have shown expression levels large number miRNAs peripheral blood, serum, exosomes isolated from are altered each other. Further warranted independently validate these findings so individual or pairs serum potential diagnostic markers for adult pediatric monitoring progression therapy.

Language: Английский

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Circulating microRNAs in Medicine

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(7), P. 3996 - 3996

Published: April 3, 2022

Circulating microRNAs (c-microRNAs, c-miRNAs), which are present in almost all biological fluids, promising sensitive biomarkers for various diseases (oncological and cardiovascular diseases, neurodegenerative pathologies, etc.), their signatures accurately reflect the state of body. Studies expression microRNA markers show that they can enable a wide range to be diagnosed before clinical symptoms manifested, help assess patient’s response therapy order correct personalize treatments. This review discusses latest trends uses miRNAs diagnosing treating viral non-viral. It is concluded exogenous used as high-precision therapeutic agents these purposes.

Language: Английский

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miR-155 as an Important Regulator of Multiple Sclerosis Pathogenesis. A Review

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(9), P. 4332 - 4332

Published: April 21, 2021

Multiple sclerosis (MS) is a chronic, immune-mediated disease and the leading cause of disability among young adults. MicroRNAs (miRNAs) are involved in post-transcriptional regulation gene expression. Of them, miR-155 crucial regulator inflammation plays role modulating autoimmune response MS. blood-brain barrier (BBB) disruption via down-regulation key junctional proteins under inflammatory conditions. It drives demyelination processes by contributing to, e.g., microglial activation, polarization astrocytes, CD47 protein affecting transcription factors. has huge impact on development neuropathic pain indirectly influences regulatory T (Treg) cell differentiation alleviation hypersensitivity. This review also focused neuropsychiatric symptoms appearing as result disease-associated stressors, brain atrophy, pro-inflammatory Recent studies revealed regulating anxiety, stress, hippocampus, treatment-resistant depression. Inhibition expression was demonstrated to be effective preventing pathophysiology aimed support better understanding great dysregulation various aspects MS highlight future perspectives for this molecule.

Language: Английский

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Serum miRNA Signature in Rheumatoid Arthritis and “At-Risk Individuals”

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12

Published: March 3, 2021

Background MicroRNAs (miRNAs) are small non-coding RNAs which have been implicated as potential biomarkers or therapeutic targets in autoimmune diseases. This study examines circulatory miRNAs RA patients and further investigates if a serum miRNA signature precedes clinical manifestations of disease arthralgia “at-risk individuals”. Methods Serum was collected from HC subjects (N = 20), 50), 10), addition to subgroup the post-methotrexate (MTX) 18). The FirePlex Immunology-V2 panel selected for multiplex analysis 68 each sample. DNA intelligent (DIANA)-mirPath Ingenuity Pathway Analysis (IPA) software were used predict pathways targeted by dysregulated miRNAs. Results 8 (miR-126-3p, let-7d-5p, miR-431-3p, miR-221-3p, miR-24-3p, miR-130a-3p, miR-339-5p, let-7i-5p) significantly elevated compared (all p < 0.01) 1 (miR-17-5p) lower (p 0.01). High specificity sensitivity determined receiver operating characteristic (ROC) curve analysis. Both miR-339-5p let-7i-5p reduced post-MTX (both MiR-126-3p, miR-130a-3p also “at risk” developing 0.05) HC. IPA this identified downstream including key transcription factors NF-κB, STAT-1, STAT-3, cytokines IL-1β, TNF-α, matrix-metalloproteases all importantly associated with pathogenesis. Conclusion six that altered both individuals,” potentially regulate involved regulating inflammation. These may predictive onset early progression.

Language: Английский

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Light-activated CRISPR-Cas12a for amplified imaging of microRNA in cell cycle phases at single-cell levels

Science Advances, Journal Year: 2024, Volume and Issue: 10(30)

Published: July 24, 2024

An ortho-nitrobenzyl phosphate ester-caged nucleic acid hairpin structure coupled to the CRISPR-Cas12a complex is introduced as a functional reaction module for light-induced activation of (LAC12a) machinery toward amplified fluorescence detection microRNA-21 (miRNA-21). The LAC12a applied selective, in vitro sensing miRNA-21 and intracellular imaging different cell lines. system used image cycle phases MCF-7 cells. Moreover, integrated cells enables two-photon laser confocal microscopy-assisted, light-stimulated spatiotemporal, selective at single-cell level evaluation relative expression levels distinct phases. method implemented map distribution an array single

Language: Английский

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A comprehensive review on miR-146a molecular mechanisms in a wide spectrum of immune and non-immune inflammatory diseases

Immunology Letters, Journal Year: 2020, Volume and Issue: 227, P. 8 - 27

Published: Aug. 15, 2020

Language: Английский

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