The Role of DNA Methylation in Zebrafish Models of CNS Diseases DOI

L. V. Yushko,

A. D. Shevlyakov,

M. A. Romazeva

et al.

Российский физиологический журнал им  И  М  Сеченова, Journal Year: 2024, Volume and Issue: 110(5), P. 661 - 679

Published: Oct. 19, 2024

DNA methylation plays an important role in the regulation of gene expression. Disturbances this process brain cause various neurological diseases, including autism, schizophrenia and mood disorders. Zebrafish (Danio rerio) are a promising model organism biomedicine. Given high genetic physiological homology with humans, studying genome deficits zebrafish can help to clarify molecular processes underlying etiology pathogenesis as well develop novel therapies. Here, we discuss mechanisms diseases associated its dysregulation their pharmacological models zebrafish. We also evaluate limitations possible directions for further research field. Mounting evidence summarized here supports effective elucidating pathologies impaired methylation.

Language: Английский

Interacted toxic mechanisms of ochratoxin A and tricyclazole on the zebrafish (Danio rerio) DOI Creative Commons
Yanhua Wang,

Meihua Deng,

Chen Chen

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 326, P. 138429 - 138429

Published: March 16, 2023

Language: Английский

Citations

11
Experimental PTSD Models in Zebrafish: A Systematic Review of Behavioral, Neurochemical, and Molecular Outcomes DOI Creative Commons
Alexey Sarapultsev, Evgeni Gusev, Desheng Hu

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 456 - 456

Published: April 23, 2025

Post-traumatic stress disorder (PTSD) is a complex psychiatric condition characterized by persistent behavioral and neurobiological alterations following trauma. Although rodent models are commonly used to study PTSD, zebrafish (Danio rerio) have emerged as promising alternative due their genetic similarity humans, conserved response systems, high-throughput capabilities. This systematic review evaluates 33 experimental studies on PTSD models, focusing behavioral, neurochemical, molecular outcomes. Chronic unpredictable (CUS/UCS) paradigms of 14–15 days were identified the most reliable for inducing PTSD-like phenotypes, consistently resulting in anxiety-like behaviors, cortisol dysregulation, gene expression changes. In contrast, acute produced transient effects, social defeat showed methodological variability. frequently demonstrated neurotransmitter imbalances, oxidative stress, upregulation inflammatory neuroplasticity-related genes. However, literature revealed challenges, including protocol heterogeneity, limited sex-specific analyses, constraints longitudinal biomarker tracking. Future directions include epigenetic profiling, environmental standardization, cross-species validation. When with rigor, offer powerful translationally relevant platform mechanisms screen novel interventions.

Language: Английский

Citations

0
Zebrafish Model in Illuminating the Complexities of Post-Traumatic Stress Disorders: A Unique Research Tool DOI Open Access
Raed M. Al‐Zoubi, Haya Abuhijleh, Ahmad Zarour

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4895 - 4895

Published: April 30, 2024

Post-traumatic stress disorder (PTSD) is a debilitating psychological condition that may develop in certain individuals following exposure to life-threatening or traumatic events. Distressing symptoms, including flashbacks, are characterized by disrupted responses, fear, anxiety, avoidance tendencies, and disturbances sleep patterns. The enduring effects of PTSD can profoundly impact personal familial relationships, as well social, medical, financial stability. prevalence varies among different populations influenced the nature event. Recently, zebrafish have emerged valuable model organism studying various conditions disorders. Zebrafish display robust behavioral patterns be effectively quantified using advanced video-tracking tools. Due their relatively simple nervous system compared humans, particularly suited for investigations. These unique characteristics make an appealing exploring underlying molecular genetic mechanisms govern behavior, thus offering powerful comparative platform gaining deeper insights into PTSD. This review article aims provide updates on pathophysiology responses associated with stress. Additionally, it highlights significance behavior tool comprehending better. By leveraging organism, researchers potentially uncover novel therapeutic interventions treatment contribute more comprehensive understanding this complex condition.

Language: Английский

Citations

3
Combined toxicity of aflatoxin B1 and tebuconazole to the embryo development of zebrafish (Danio rerio) DOI

Xuehua An,

Shanshan Di, Xinquan Wang

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 346, P. 140612 - 140612

Published: Nov. 4, 2023

Language: Английский

Citations

7
Impacts of co-exposure to zearalenone and trifloxystrobin on the enzymatic activity and gene expression in zebrafish DOI Creative Commons
Tao Cang,

Changxing Wu,

Chen Chen

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 256, P. 114860 - 114860

Published: April 1, 2023

Although humans and animals are usually exposed to combinations of toxic substances, little is known about the interactive toxicity mycotoxins farm chemicals. Therefore, we can not precisely evaluate health risks combined exposure. In present work, using different approaches, examined impacts zearalenone trifloxystrobin on zebrafish (Danio rerio). Our findings showed that lethal embryonic fish with a 10-day LC50 0.59 mg L-1 was lower than (0.037 L-1). Besides, mixture triggered acute synergetic fish. Moreover, contents CAT, CYP450, VTG were distinctly altered in most single exposures. Transcriptional levels 23 genes involved oxidative response, apoptosis, immune, endocrine systems determined. results implied eight (cas9, apaf-1, bcl-2, il-8, trb, vtg1, erβ1, tg) displayed greater changes when compared corresponding individual indicated performing risk assessment based impact rather dosage response these chemicals more accurate. Nevertheless, further investigations still necessary reveal modes action mycotoxin pesticide alleviate their effects human health.

Language: Английский

Citations

4
Exposure effects of synthetic glucocorticoid drugs on skeletal developmental and immune cell function in zebrafish DOI Creative Commons
Charles M. Hamilton, Matthew J. Winter, Jonathan S. Ball

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176781 - 176781

Published: Oct. 10, 2024

Synthetic glucocorticoids (GCs) are used to treat a wide range of human health conditions and as such frequently detected in the aquatic environment. This, together with highly conserved nature glucocorticoid system across vertebrates means that potential for biological effects GCs fish is relatively high. Here, we found exposure zebrafish (Danio rerio) environmentally relevant concentrations 4 most widely synthetic (beclomethasone dipropionate, budesonide, fluticasone propionate, prednisolone), from 0 days post fertilisation (dpf), resulted no on embryo-larval development or bone cartilage formation. However, after equivalents therapeutic plasma levels, developmental abnormalities were observed included pericardial oedema, blood pooling alterations jaw cartilage. Furthermore, using double transgenic osteoblast chondrocyte reporter line, up 10 dpf lower all compounds at, above, concentrations. In case beclomethasone reduction intercranial distance was at concentration 0.1 μg/L. Using further lines fluorescently tagged neutrophils macrophages, also show embryo-larvae (0-4 dpf) tested altered immune cell migration, but only high Collectively, our findings GC impacts development, function, skeletal formation, predominantly greater than those currently reported Despite this, however, it suggested studies longer times, mixtures multiple (many act via same mechanism action) warranted before can confidently assert these commonly contaminants do not pose risk wild.

Language: Английский

Citations

1
The Role of DNA Methylation in Zebrafish Models of CNS Diseases DOI Creative Commons

L. V. Yushko,

A. D. Shevlyakov,

M. A. Romazeva

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 60(3), P. 973 - 987

Published: May 1, 2024

Abstract DNA methylation plays an important role in the regulation of gene expression. Its deficits brain cause various neurological diseases, including autism, schizophrenia and mood disorders. The zebrafish ( Danio rerio ) is a promising model organism biomedicine. Given its high genetic physiological homology with humans, studying genome can help elucidate molecular processes underlying etiology pathogenesis as well develop novel therapies. Here, we discuss mechanisms diseases associated dysregulation their pharmacological models zebrafish. We also evaluate limitations possible directions for further research this field. Mounting evidence summarized here supports effective elucidating pathologies compromised methylation.

Language: Английский

Citations

0
The Role of DNA Methylation in Zebrafish Models of CNS Diseases DOI

L. V. Yushko,

A. D. Shevlyakov,

M. A. Romazeva

et al.

Российский физиологический журнал им  И  М  Сеченова, Journal Year: 2024, Volume and Issue: 110(5), P. 661 - 679

Published: Oct. 19, 2024

DNA methylation plays an important role in the regulation of gene expression. Disturbances this process brain cause various neurological diseases, including autism, schizophrenia and mood disorders. Zebrafish (Danio rerio) are a promising model organism biomedicine. Given high genetic physiological homology with humans, studying genome deficits zebrafish can help to clarify molecular processes underlying etiology pathogenesis as well develop novel therapies. Here, we discuss mechanisms diseases associated its dysregulation their pharmacological models zebrafish. We also evaluate limitations possible directions for further research field. Mounting evidence summarized here supports effective elucidating pathologies impaired methylation.

Language: Английский

Citations

0