Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,
Journal Year:
2024,
Volume and Issue:
43(6), P. 153 - 159
Published: Dec. 1, 2024
Congenital
hypothyroidism
(CH)
is
a
major
health
issue
that
can
lead
to
intellectual
disability
if
not
detected
and
treated
earlier.
The
preliminary
screening
program
for
neonatal
CH
in
Indonesia
gave
provisional
incidence
of
1:2513.
Newborn
using
dried
blood
spot
sample
the
standard
method
detection,
but
it
has
limitations.
Despite
proven
benefits
screening,
still
faces
significant
challenges
implementing
nationwide
program.
This
study
aimed
develop
more
sensitive
accessible
by
creating
monoclonal
antibodies
(mAbs)
against
thyroid-stimulating
hormone
(TSH).TSH
protein
was
isolated
from
newborn
cord
confirmed
Western
blot
analysis.
Mice
were
immunized
with
purified
TSH,
hybridoma
cell
lines
generated
through
fusion.
Hybridoma
supernatants
screened
TSH-specific
ELISA.
mAb
highest
titer
dialysis.
analysis
presence
TSH
fraction
at
28
kDa.
Immunized
mice
showed
increase
antibody
compared
control
group.
clones
secreting
high-titer
identified.
research
successfully
produced
mAbs
it.
They
enable
development
rapid,
point-of-care
diagnostic
tests,
such
as
lateral
flow
immunoassays,
which
provide
results
within
minutes.
It
will
lay
groundwork
innovative
tools
significantly
improve
early
diagnosis
treatment
this
condition,
particularly
resource-limited
settings.
Lab on a Chip,
Journal Year:
2024,
Volume and Issue:
24(15), P. 3626 - 3650
Published: Jan. 1, 2024
This
review
covers
recent
advances
in
sample
preparation
and
detection
methods
for
point-of-care
testing,
aiming
to
accelerate
progress
towards
at-home
health
monitoring
personalized
healthcare
the
future.
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(16), P. 19368 - 19376
Published: Aug. 1, 2024
With
the
increasing
misuse
of
antibiotic
drugs,
convenient
and
ultrasensitive
detection
chloramphenicol
(CAP)
is
essential
for
ensuring
food
safety
human
health.
In
this
study,
a
comprehensive
platform
was
developed
integrating
discrimination
capabilities
by
combining
surface-enhanced
Raman
scattering
(SERS)
with
lateral
flow
immunoassay
(LFIA)
technology,
along
support
vector
machine
(SVM)
model
data
analysis,
to
detect
CAP
residues
in
aquatic
products.
The
CAP-specific
antibody-modified
gold
nanostars
(Au
NSs)
were
synthesized
as
SERS–LFIA
nanoprobes,
achieving
an
ultralow
limit
(LOD)
10
pg/mL
theoretical
LOD
1.6
simulated
samples.
This
sensitivity
surpasses
that
currently
available
commercial
strips
demonstrates
high
specificity.
Additionally,
automated
exhibited
(95.83%)
specificity
(96.43%)
well
accuracy
96%
on
test
set,
marking
8%
improvement
over
traditional
intensity
methods.
simple,
fast,
technique
eliminates
need
sample
pretreatment,
offering
promising
approach
trace
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(8), P. 786 - 786
Published: Aug. 3, 2023
The
COVID-19
pandemic
has
spurred
demand
for
efficient
and
rapid
diagnostic
tools
that
can
be
deployed
at
point
of
care
to
quickly
identify
infected
individuals.
Existing
detection
methods
are
time
consuming
they
lack
sensitivity.
Point-of-care
testing
(POCT)
emerged
as
a
promising
alternative
due
its
user-friendliness,
rapidity,
high
specificity
Such
tests
conveniently
conducted
the
patient’s
bedside.
Immunodiagnostic
offer
identification
positive
cases
urgently
required.
Quantum
dots
(QDs),
known
their
multimodal
properties,
have
shown
potential
in
terms
combating
or
inhibiting
virus.
When
coupled
with
specific
antibodies,
QDs
enable
highly
sensitive
viral
antigens
patient
samples.
Conventional
lateral
flow
immunoassays
(LFAs)
been
widely
used
simplicity,
low
cost,
portability.
However,
often
sensitivity
required
accurately
detect
loads.
dot
(QD)-based
alternative,
offering
significant
advancements
specificity.
Moreover,
immunoassay
(LFIA)
method,
which
fulfils
POCT
standards,
gained
popularity
diagnosing
COVID-19.
This
review
focuses
on
recent
QD-based
LFIA
diagnosis.
Strategies
enhance
using
explored,
underlying
principles
elucidated.
benefits
method
highlighted,
published
performance
diagnostics
is
examined.
Overall,
integration
quantum
holds
immense
promise
revolutionizing
detection,
treatment,
prevention,
convenient
effective
approach
combat
pandemic.
Colorimetric
lateral
flow
assays
(LFAs)
are
widely
used
for
detecting
analytes
through
color
changes
displayed
by
conjugates
on
a
test
strip,
typically
made
of
materials
such
as
nitrocellulose
(NC)
membranes.
However,
significant
number
conjugate
nanoparticles
become
trapped
within
the
membrane's
porous
structure,
preventing
full
display
information.
Here,
gelation
NC
membrane
is
achieved
introducing
dimethyl
sulfoxide
(DMSO)
into
an
LFA
resulting
in
structural
and
high
transparency
membrane,
exposing
red-colored
Au
transparent
portable
DMSO/NC
membrane.
Under
optimized
conditions
(16
μL
50
vol
%
DMSO
water
heating
strip
at
80
°C
8
min),
becomes
highly
transparent,
achieving
77.42%
transmittance
while
maintaining
its
capability
to
effectively
perform
LFAs
cardiac
troponin
I
(cTnI)
both
buffer
solutions
clinical
samples.
We
anticipate
that
this
method
holds
promise
advancing
fundamental
studies
has
potential
applications
healthcare
environmental
monitoring.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(2), P. 648 - 648
Published: Jan. 14, 2025
Osteocalcin
is
a
useful
biomarker
for
bone
formation
and
bone-related
diseases.
KTM219
an
anti-osteocalcin
C-terminal
peptide
antibody.
The
single-chain
variable
region
(scFv)
antigen-binding
fragment
(Fab)
of
are
applicable
to
the
Quenchbody
(Q-body)
immunoassay.
Q-body
new
type
fluorescent
immunosensor,
which
scFv
or
Fab
labeled
with
dye.
When
binds
its
antigen,
fluorescence
intensity
increases.
highly
sensitive
detection
antigens
by
changes
in
performed
single
step
mixing
sample
reagent.
In
this
study,
reveal
recognition
mechanism
antibody
discuss
structural
basis
Q-body,
we
solved
crystal
structures
complex
antigen
osteocalcin
(BGP-C7).
Also,
structure
grown
presence
dye,
carboxytetramethylrhodamine
(TAMRA);
however,
tightly
bound
TAMRA
was
not
found
electron
density
map.
We
predicted
binding
sites
pocket
docking
simulations.
These
results
support
proposed
mechanism.
would
be
further
development
improvement
immunosensors.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 197 - 197
Published: March 20, 2025
This
article
explores
the
development
and
application
of
innovative
piezoelectric
sensors
in
point-of-care
diagnostics.
It
highlights
significance
bedside
tests,
such
as
lateral
flow
electrochemical
providing
rapid
accurate
results
directly
at
patient’s
location.
paper
delves
into
principles
assays,
emphasizing
their
ability
to
detect
disease-related
biomarkers
through
mechanical
stress-induced
electrical
signals.
Various
applications
chemosensors
biosensors
are
discussed,
including
use
detection
cancer
biomarkers,
pathogens,
other
health-related
analytes.
also
addresses
integration
materials
with
advanced
sensing
technologies
improve
diagnostic
accuracy
efficiency,
offering
a
comprehensive
overview
current
advances
future
directions
medical
Nano Letters,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 18, 2025
The
application
of
conventional
lateral
flow
immunoassay
(LFIA)
remains
challenging
due
to
its
single
signal
readout
and
poor
sensitivity.
In
this
work,
we
developed
J-aggregated
fluorescence
nanoparticles
(JNPs)
with
multichromatic
performance
employed
them
as
reporters
for
the
simultaneous
sensitive
detection
T-2
AFB1
toxins
on
LFIA
platform.
Upon
strong
confinement
effect
nanoparticles,
emission
region
JNPs
was
red-shifted
from
∼545
∼620
nm,
exhibiting
a
J-aggregation
phenomenon.
Subsequently,
two
green
red
were
development
multiplexed
platform
(JNPs-LFIA)
on-site
quantitative
toxins,
limits
0.645
ng
mL-1
0.0035
mL-1,
which
3.5-fold
12.2-fold
lower
than
those
AuNPs-LFIA,
respectively.
Overall,
work
provides
an
efficient
strategy
design
immunoprobes
promotes
in
biosensing
application.
