Fluorescent probes - illuminate the interplay network between lipid droplets and other organelles

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 510, P. 215792 - 215792

Published: March 28, 2024

Language: Английский

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Biomedical applications of NIR-II organic small molecule fluorescent probes in different organs

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 519, P. 216122 - 216122

Published: Aug. 6, 2024

Language: Английский

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Precise subcellular targeting approaches for organelle-related disorders

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 212, P. 115411 - 115411

Published: July 19, 2024

Language: Английский

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Reversible Fluorescent Probes for Dynamic Imaging of Liver Ischemia-Reperfusion Injury

Accounts of Chemical Research, Journal Year: 2024, Volume and Issue: 57(17), P. 2594 - 2605

Published: Aug. 20, 2024

ConspectusHepatic ischemia-reperfusion injury (HIRI) is an inevitable complication of clinical surgeries such as liver resection or transplantation, often resulting in postoperative dysfunction, hepatic failure up to 13% postresection patients, and early graft 11-18% transplantation patients. HIRI involves a series biochemical events triggered by abnormal alterations multiple biomarkers, characterized short lifespans, dynamic changes, subcellular regional distribution, multicollaborative regulation. However, traditional diagnosis, including serology, imaging, puncture biopsy, suffers from low sensitivity, poor resolution, hysteresis, which hinder effective monitoring markers. Thus, address the unique properties markers, there pressing demand for developing novel detection strategies that are highly selective, transiently responsive, dynamically reversible, organelle-targeted, capable simultaneous multicomponent analysis.Optical probe-based fluorescence imaging powerful tool real-time biomarkers with advantages high noninvasiveness, rapid analysis, high-fidelity acquisition spatiotemporal information on signaling molecules compared conventional methods. Moreover, growing continuous probes reversible features receiving more attention. Importantly, can not only monitor fluctuations marker concentrations but also distinguish between transient bursts markers during physiological long-term sustained increases pathological levels. This effectively avoid false-positive test results, addition, be reutilized green economical features. Therefore, our team has employed various methods design optical HIRI. We proposed recognition based specific reactions interactions detect changes Given biomarkers' organelles synergistic regulatory HIRI, bifunctional exploited, organelle-targeted detection. With these strategies, we have tailored variety fluorescent reactive oxygen/nitrogen species (O

Language: Английский

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Clog P‐Guided Development of Multi‐Colored Buffering Fluorescent Probes for Super‐Resolution Imaging of Lipid Droplet Dynamics

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Super‐resolution fluorescence imaging of live cells increasingly demands fluorescent probes capable multi‐color and long‐term dynamic imaging. Understanding the mechanisms probe‐target recognition is essential for engineered development such probes. In this study, it discovered that molecular lipid solubility parameter, Clog P , determines staining performance dyes on droplets (LDs). Fluorescent with values between 2.5 4 can form buffering pools outside LDs, replacing photobleached within LDs to maintain constant intensity in thereby enabling super‐resolution LDs. Guided by four different colored LD spanning visible light spectrum have been developed. Using Structured Illumination Microscopy (SIM), role dynamics tracked during cellular ferroptosis secretion, storage, degradation overexpressed ACSL3 proteins. It found serve as storage sites these proteins through membrane fusion, further degrade via interactions organelles like lysosomes or lipophagy, maintaining homeostasis.

Language: Английский

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Spatiotemporal Dynamic Monitoring of Protein Conformation Changes by Super-resolution Imaging

Dyes and Pigments, Journal Year: 2025, Volume and Issue: 236, P. 112689 - 112689

Published: Feb. 3, 2025

Language: Английский

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A novel FRET-based fluorescent probe capable of simultaneously imaging lipid droplets and the endoplasmic reticulum with two distinct fluorescence signals in HeLa cells

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126262 - 126262

Published: April 1, 2025

Language: Английский

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Luminescent iridium(iii)–peptide bioconjugates for bioanalytical and biomedical applications

Inorganic Chemistry Frontiers, Journal Year: 2024, Volume and Issue: 11(12), P. 3400 - 3417

Published: Jan. 1, 2024

We describe the interactions between iridium( iii ) complexes and peptides emerging applications of )–peptide bioconjugates in biomedical fields, summarizing commenting on their widespread for bioimaging therapy.

Language: Английский

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Molecular probes for super-resolution imaging of drug dynamics

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 210, P. 115330 - 115330

Published: May 10, 2024

Language: Английский

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Unveiling intracellular phase separation: advances in optical imaging of biomolecular condensates

Trends in Biochemical Sciences, Journal Year: 2024, Volume and Issue: 49(10), P. 901 - 915

Published: July 20, 2024

Language: Английский

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