Eukaryotic core promoters and the functional basis of transcription initiation

Nature Reviews Molecular Cell Biology, Journal Year: 2018, Volume and Issue: 19(10), P. 621 - 637

Published: June 26, 2018

Language: Английский

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Spatial organization of chromatin domains and compartments in single chromosomes

Science, Journal Year: 2016, Volume and Issue: 353(6299), P. 598 - 602

Published: July 22, 2016

Spatial organization inside the nucleus In eukaryotic cells, DNA is packaged into a complex macromolecular structure called chromatin. Wang et al. have developed an imaging method to map position of multiple regions on individual chromosomes, and results confirm that chromatin organized large contact domains TADS (topologically associating domains). Unexpectedly, though, folding deviates from classical fractal-globule model at length scales. Science , this issue p. 598

Language: Английский

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Pioneer transcription factors in cell reprogramming

Genes & Development, Journal Year: 2014, Volume and Issue: 28(24), P. 2679 - 2692

Published: Dec. 15, 2014

A subset of eukaryotic transcription factors possesses the remarkable ability to reprogram one type cell into another. The that fate are invariably those crucial for initial programming in embryonic development. To elicit or reprogramming, must be able engage genes developmentally silenced and inappropriate expression original cell. Developmentally typically embedded "closed" chromatin is covered by nucleosomes not hypersensitive nuclease probes such as DNase I. Biochemical genomic studies have shown with highest reprogramming activity often special their target sites on nucleosomal DNA, thus behaving "pioneer factors" initiate events closed chromatin. Other appear dependent pioneer engaging However, certain domains which occluded higher-order structures, heterochromatin, resistant factor binding. Understanding means can how heterochromatin prevent binding promises advance our fates at will topic this review.

Language: Английский

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Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

Nature, Journal Year: 2019, Volume and Issue: 572(7770), P. 543 - 548

Published: Aug. 1, 2019

Language: Английский

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Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells

Science, Journal Year: 2015, Volume and Issue: 347(6225), P. 1010 - 1014

Published: Feb. 13, 2015

Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses. Binding sites for key lineage factors were overrepresented each system. Our data support highly generalizable model which enhancer event successive waves change during or activation.

Language: Английский

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Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin

Cell, Journal Year: 2020, Volume and Issue: 182(6), P. 1641 - 1659.e26

Published: Aug. 20, 2020

Language: Английский

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Enhancer–core-promoter specificity separates developmental and housekeeping gene regulation

Nature, Journal Year: 2014, Volume and Issue: 518(7540), P. 556 - 559

Published: Dec. 12, 2014

Language: Английский

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Dynamic interplay between enhancer–promoter topology and gene activity

Nature Genetics, Journal Year: 2018, Volume and Issue: 50(9), P. 1296 - 1303

Published: July 23, 2018

Language: Английский

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Insulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control

Cell, Journal Year: 2016, Volume and Issue: 167(5), P. 1188 - 1200

Published: Nov. 1, 2016

Language: Английский

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Visualizing DNA folding and RNA in embryos at single-cell resolution

Nature, Journal Year: 2019, Volume and Issue: 568(7750), P. 49 - 54

Published: March 18, 2019

Language: Английский

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