Published: Aug. 7, 2024
Language: Английский
Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)
Published: Jan. 13, 2025
The tumor microenvironment (TME) is integral to cancer progression, impacting metastasis and treatment response. It consists of diverse cell types, extracellular matrix components, signaling molecules that interact promote growth therapeutic resistance. Elucidating the intricate interactions between cells TME crucial in understanding progression challenges. A critical process induced by epithelial-mesenchymal transition (EMT), wherein epithelial acquire mesenchymal traits, which enhance their motility invasiveness progression. By targeting various components TME, novel investigational strategies aim disrupt TME's contribution EMT, thereby improving efficacy, addressing resistance, offering a nuanced approach therapy. This review scrutinizes key players emphasizing avenues therapeutically components. Moreover, article discusses implications for resistance mechanisms highlights current toward modulation along with potential caveats.
Language: Английский
Citations
16
Hepatology International, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 20, 2025
Language: Английский
Citations
1
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(5)
Published: March 1, 2025
Triple-negative breast cancer (TNBC) is a highly aggressive form of with poor prognosis and high mortality. The chemotherapeutic regimen remains the predominant treatment modality for TNBC in current clinical practice. However, chemotherapy resistance significantly complicates development an effective regimen. Furthermore, immunosuppressive microenvironment contributes to enhanced tumour aggressiveness. Consequently, understanding its mechanisms progression finding therapeutic interventions crucial. Recent evidence has identified extracellular vesicles (EVs) as key mediators cell-to-cell communication immune regulation. In view remarkable ability EVs transfer active molecules, such proteins nucleic acids, from parental recipient cells, they are regarded promising biomarker novel drug delivery system. this review, we provide overview how derived cells play role regulating progression. We also discuss potential regulation their application strategies markers TNBC. knowledge gained studying EV-mediated could lead targeted therapies improve patient outcomes.
Language: Английский
Citations
0
Pharmacological Research, Journal Year: 2024, Volume and Issue: 208, P. 107352 - 107352
Published: Aug. 13, 2024
A cutting-edge approach in cell-based immunotherapy for combating resistant cancer involves genetically engineered chimeric antigen receptor T (CAR-T) lymphocytes. In recent years, these therapies have demonstrated effectiveness, leading to their commercialization and clinical application against certain types of cancer. However, CAR-T therapy faces limitations, such as the immunosuppressive tumour microenvironment (TME) that can render cells ineffective, adverse side effects therapy, including cytokine release syndrome (CRS). Extracellular vesicles (EVs) are a diverse group membrane-bound particles released into extracellular environment by virtually all cell types. They essential intercellular communication, transferring cargoes proteins, lipids, various RNAs, DNA fragments target cells, traversing biological barriers both locally systemically. EVs play roles numerous physiological processes, with those from immune non-immune capable modulating system through activation or suppression. Leveraging this capability enhance could represent significant advancement overcoming its current limitations. This review examines landscape explores potential role augmenting therapeutic efficacy.
Language: Английский
Citations
3
PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18428 - e18428
Published: Nov. 11, 2024
Background Cancer-associated fibroblasts (CAFs) and hepatocellular carcinoma (HCC) cells interact to promote HCC progression, but the underlying mechanisms remain unclear. Serpin family E member 1 (SERPINE1) has conflicting roles in HCC, microRNAs (miRNAs) are known regulate tumor progression through intercellular communication. Therefore, we investigated potential involvement of miRNA/SERPINE1 axis crosstalk between CAFs cells. Methods In this study, candidate miRNAs targeting SERPINE1 3′ UTR were predicted using multiple miRNA databases. The mRNA expression Huh7 was assessed after co-culture with RT-qPCR. cell proliferation invasion detected siRNA. functions CAF-derived miR-642a-3p/SERPINE1 examined CCK-8, wound healing, transwell assays, western blot, dual-luciferase reporter assays. Moreover, a orthotopic xenograft model used investigate contribution miR-642a-3p knockdown HCC. Results decreased, while increased co-cultured CAFs. enhanced as well expression. overexpression promoted migration, invasion, epithelial-mesenchymal transition (EMT) by SERPINE1, yielded opposite effect. Rescue experiments confirmed that attenuated inhibitory effects on EMT Importantly, suppressed growth liver tumors. Conclusion facilitated cells, suggesting can be therapeutic target for
Language: Английский
Citations
3
MedComm, Journal Year: 2024, Volume and Issue: 5(8)
Published: July 15, 2024
Exosomes are nanoscale vesicles of cellular origin. One the main characteristics exosomes is their ability to carry a wide range biomolecules from parental cells, which important mediators intercellular communication and play an role in physiological pathological processes. have advantages biocompatibility, low immunogenicity, biodistribution. As researchers' understanding has increased, various strategies been proposed for use diagnosing treating diseases. Here, we provide overview biogenesis composition exosomes, describe relationship between disease progression, focus on as biomarkers early screening, monitoring, guiding therapy refractory diseases such tumors neurodegenerative We also summarize current applications especially engineered efficient drug delivery, targeted therapies, gene immune vaccines. Finally, challenges potential research directions clinical application discussed. In conclusion, emerging molecule that can be used diagnosis treatment diseases, combined with multidisciplinary innovative solutions, will applications.
Language: Английский
Citations
2
Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Journal Year: 2024, Volume and Issue: 33(1), P. 133 - 148
Published: Sept. 13, 2024
Lung cancer is a life-threatening disease that occurs worldwide, but especially common in China. The crucial role of the tumour microenvironment (TME) non-small cell lung (NSCLC) has attracted recent attention. Cancer-associated fibroblasts (CAFs) are main factors contribute to TME function, and CAF exosomes closely linked NSCLC. expression levels miR-3124-5p Toll-interacting protein (TOLLIP) were analysed by bioinformatics prediction combined with RT-qPCR/Western Blot detection. Fibroblasts isolated identified from clinical NSCLC tissues. Transmission electron microscopy Western used identify these cells. Changes proliferation (CCK-8 clone formation), migration (wound healing), invasion (transwell) cells measured. Luciferase reporter test was applied clarify binding TOLLIP. TOLLIP/TLR4/MyD88/NF-κB pathway proteins determined using blot analysis. MiR-3124-5p overexpressed tissues dramatically enriched CAF-derived exosomes. Cellular experiments revealed CAFs delivered into via exosomes, stimulating progression. acted as sponge negatively regulate TOLLIP expression, which activated TLR4/MyD88/NF-κB axis promote occurrence development Functional salvage tests performed determine whether CAF-exosome-derived plays pro-cancer affecting signalling pathway. These results provide an interesting direction for diagnosis therapy
Language: Английский
Citations
1
Medical Oncology, Journal Year: 2024, Volume and Issue: 42(1)
Published: Dec. 27, 2024
Language: Английский
Citations
1
Journal of Cancer Metastasis and Treatment, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 27, 2024
The tumor microenvironment (TME) of breast cancer (BC) is depicted as an immunosuppressive dwelling that comprises a myriad cell types embedded in the extracellular matrix. As one most abundant populations within TME, cancer-associated fibroblasts (CAFs) play indispensable roles increasing aggressiveness and promoting resistance to standard-of-care therapies. Extracellular vesicles (EVs) represent diverse array biological nanoparticles, encompassing exosomes, microvesicles, apoptotic bodies. In recent years, these cell-derived membranous structures have raised great interest they can encapsulate numerous cellular cargo, such proteins, lipids, miRNAs. By transmitting bioactive content recipient cells, EVs pivotal intercellular communication between CAFs cells. secreted from cells typically activate resident acquire myofibroblastic phenotype, while diffused by CAFs, turn, substantially increase progression BC. This review summarizes latest findings highlight functional role EV especially miRNAs, regulatory network. A better understanding EV-mediated cell-cell interactions crucial achieving effective treatment patients with
Language: Английский
Citations
0
Published: Aug. 7, 2024
Language: Английский
Citations
0