Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification DOI Creative Commons
Lisa U. Teufel, Jéssica Cristina dos Santos

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(4), P. 103468 - 103468

Published: Dec. 1, 2024

Lipophosphoglycan (LPG) is a macromolecule on the surface of Leishmania spp. parasites. The biochemical structure LPG varies throughout parasites' life cycle between proliferative (procyclic) and infective (metacyclic) stages, as well species strains. Here, we outline protocol for growing parasites in vitro to harvest LPG. We describe steps parasite differentiation extraction purification. has applications medical research, such trained immunity immunotherapy cancer. For complete details use execution this protocol, please refer dos Santos et al.1.

Language: Английский

Trained immunity in chronic inflammatory diseases and cancer DOI
George Hajishengallis, Mihai G. Netea, Triantafyllos Chavakis

et al.

Nature reviews. Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

4
Differential structure and immunomodulatory functions of lipophosphoglycan between Leishmania spp DOI Creative Commons
Lisa U. Teufel, Leo A. B. Joosten, Jéssica Cristina dos Santos

et al.

Immunology Letters, Journal Year: 2024, Volume and Issue: 268, P. 106885 - 106885

Published: June 18, 2024

Leishmaniasis is a collective term for several tropical, neglected diseases caused by protozoans of the species Leishmania, 20 which causing disease in humans ranging from localised self-healing lesions to chronic manifestations affect skin or inner organs. Although millions infections are accounted annually, treatment options scarce and limited medication associated with heavy side-effects increasing antibiotic resistance. Case studies point towards immunotherapy as effective alternative relying on immunomodulatory properties e.g., Bacillus Calmette-Guérin vaccine. Leishmania parasites also known modulate immune system, yet underlying macromolecules surface molecules remain widely under characterised. With this short review, we aim provide complete summary existing literature describing one most expressed molecule spp, lipophosphoglycan (LPG), shows great variability between different lifecycle stages spp. Complete characterisation LPG may aid improve development vaccination strategies, open new avenues exploit unrelated conditions.

Language: Английский

Citations

5
M28 family peptidase derived from Peribacillus frigoritolerans initiates trained immunity to prevent MRSA via the complosome-phosphatidylcholine axis DOI Creative Commons
Cheng‐Kai Zhou, Zhenzhen Liu,

Zi-Ran Peng

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: March 30, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) represents a major global health threat due to its resistance conventional antibiotics. The commensal microbiota maintains symbiotic relationship with the host, playing essential roles in metabolism, energy regulation, immune modulation, and pathogen control. Mammals harbor wide range of bacteria capable producing unique metabolites potential therapeutic properties. This study demonstrated that M28 family peptidase (M28), derived from Peribacillus frigoritolerans (P. f), provided protective effects against MRSA-induced pneumonia. enhanced phagocytosis bactericidal activity macrophages by inducing trained immunity. RNA sequencing metabolomic analyses identified CFB-C3a-C3aR-HIF-1α axis-mediated phosphatidylcholine accumulation as key mechanism for M28-induced Phosphatidylcholine, like M28, also induced To enhance M28-mediated potential, it was encapsulated liposomes (M28-LNPs), which exhibited superior immune-stimulating properties compared alone. In vivo experiments revealed M28-LNPs significantly reduced bacterial loads lung damage following MRSA infection, protection Klebsiella pneumoniae Candida albicans. We first confirmed link between complement activation immunity, offering valuable insights into treatment prevention complement-related autoimmune diseases.

Language: Английский

Citations

0
Salmonella LVR01 triggers antagonistic two-armed innate immune memory that impacts on antitumor efficacy DOI Creative Commons
Sofía Chilibroste, Jéssica Cristina dos Santos, Amy Mónaco

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 30, 2025

The current understanding of innate immune memory encompasses both trained immunity and tolerance, where cells can exhibit enhanced responsiveness or paralysis upon subsequent stimuli, respectively. Various agents induce either these responses, including β-glucan, Leishmania, BCG LPS. is a clinically approved immunotherapy for bladder cancer BCG-induced important in driving anti-tumor adaptive immunity. Salmonella also shows promise treatment, eliciting potent but with transitory effects. This led us to investigate whether LVR01, like BCG, triggers its impact on responses. Herein, we report that induces an response bone marrow cells, characterized by robust cytokine second stimulus, fashion resembles Coherently that, administration tumor implanted later time, resulting slow growth extended survival. However, vitro stimulation human monocytes murine bone-marrow derived myeloid-enriched results decreased production cytokines resembling paralysis. Overall, our suggest LVR01 responses memory, as well monocytes. These two antagonistic effects could be the basis effect treatment further investigation phenomena shed light how improve Salmonella-based immunotherapies cancer.

Language: Английский

Citations

0
Trained immunity-based Adjuvated vaccines (TIbAV) approach: β-glucans as example DOI
Miriam Angulo, Abel Ramos‐Vega, Carlos Angulo

et al.

Vaccine, Journal Year: 2025, Volume and Issue: 57, P. 127240 - 127240

Published: May 1, 2025

Language: Английский

Citations

0
Structural characteristics and anti-tumor activity of alcohol-precipitated polysaccharides from Cordyceps militaris under different ethanol concentrations DOI
Keyao Dai,

Ruo-Han Wang,

Lan Mu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134784 - 134784

Published: Aug. 14, 2024

Language: Английский

Citations

2
Therapeutic potential of trained immunity for malignant disease DOI Creative Commons
Hiroyuki Takahashi, Daibo Kojima, M. Watanabe

et al.

Immunological Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Dec. 5, 2024

Trained immunity (TI) is functional memory displayed by innate immune cells (IICs). TI facilitates rapid, non-specific responses to pathogens upon secondary challenge. It driven immunological signaling and metabolic rewriting via epigenetic alteration, triggered recognition of certain stimuli. Recently, checkpoint inhibitors have come into common use in clinical oncology settings, genetically engineered cytotoxic T comprise a potent cancer treatment strategy. However, the contributions tumor microenvironment (TME) are only beginning be uncovered. Accumulating evidence that various microorganisms vaccines convey tumoricidal ability suggest may become useful anti-cancer tool. The expected roles therapy 1) promotion proinflammatory cytokine section, 2) enhancement phagocytosis, 3) quick expansion recruitment cancer-specific TME through neoantigen presentation, 4) reversal immunosuppression TME, 5) removal associated with carcinogenesis or development. Medium- long-term durability reduce risk Recent findings on usher new aspirations for treatment.

Language: Английский

Citations

1
Determination of key hub genes in Leishmaniasis as potential factors in diagnosis and treatment based on a bioinformatics study DOI Creative Commons
Mohsen Safaei, Arash Goodarzi, Zahra Abpeikar

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 28, 2024

Language: Английский

Citations

0
Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification DOI Creative Commons
Lisa U. Teufel, Jéssica Cristina dos Santos

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(4), P. 103468 - 103468

Published: Dec. 1, 2024

Lipophosphoglycan (LPG) is a macromolecule on the surface of Leishmania spp. parasites. The biochemical structure LPG varies throughout parasites' life cycle between proliferative (procyclic) and infective (metacyclic) stages, as well species strains. Here, we outline protocol for growing parasites in vitro to harvest LPG. We describe steps parasite differentiation extraction purification. has applications medical research, such trained immunity immunotherapy cancer. For complete details use execution this protocol, please refer dos Santos et al.1.

Language: Английский

Citations

0